Dynamics at the serine loop underlie differential affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing
Abstract
Mammalian circadian rhythms are generated by a transcription-based feedback loop in which CLOCK:BMAL1 drives transcription of its repressors (PER1/2, CRY1/2), which ultimately interact with CLOCK:BMAL1 to close the feedback loop with ~24 hr periodicity. Here we pinpoint a key difference between CRY1 and CRY2 that underlies their differential strengths as transcriptional repressors. Both cryptochromes bind the BMAL1 transactivation domain similarly to sequester it from coactivators and repress CLOCK:BMAL1 activity. However, we find that CRY1 is recruited with much higher affinity to the PAS domain core of CLOCK:BMAL1, allowing it to serve as a stronger repressor that lengthens circadian period. We discovered a dynamic serine-rich loop adjacent to the secondary pocket in the photolyase homology region (PHR) domain that regulates differential binding of cryptochromes to the PAS domain core of CLOCK:BMAL1. Notably, binding of the co-repressor PER2 remodels the serine loop of CRY2, making it more CRY1-like and enhancing its affinity for CLOCK:BMAL1.
- Authors:
-
[2];
[3];
[5];
[1];
[3];
[2];
[8]
- Univ. of California, Santa Cruz, CA (United States)
- Nagoya Univ. (Japan)
- The Scripps Research Inst., La Jolla, CA (United States)
- Univ. of Washington, Seattle, WA (United States)
- Kyoto Univ. (Japan)
- Univ. of Washington, Seattle, WA (United States); Howard Hughes Medical Inst., Seattle, WA (United States)
- Nagoya Univ. (Japan); RIKEN Center for Computational Science, Kobe (Japan)
- Univ. of California, Santa Cruz, CA (United States); Univ. of California, San Diego, CA (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH); Cancer Research Coordinating Committee
- OSTI Identifier:
- 1644118
- Grant/Contract Number:
- R01 GM107069; DP2 EB020402; F31 CA189660; S10 OD021634; CRN-15–380548
- Resource Type:
- Accepted Manuscript
- Journal Name:
- eLife
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 02, 2020; Journal ID: ISSN 2050-084X
- Publisher:
- eLife Sciences Publications, Ltd.
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Fribourgh, Jennifer L., Srivastava, Ashutosh, Sandate, Colby R., Michael, Alicia K., Hsu, Peter L., Rakers, Christin, Nguyen, Leslee T., Torgrimson, Megan R., Parico, Gian Carlo G., Tripathi, Sarvind, Zheng, Ning, Lander, Gabriel C., Hirota, Tsuyoshi, Tama, Florence, and Partch, Carrie L. Dynamics at the serine loop underlie differential affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing. United States: N. p., 2020.
Web. doi:10.7554/elife.55275.
Fribourgh, Jennifer L., Srivastava, Ashutosh, Sandate, Colby R., Michael, Alicia K., Hsu, Peter L., Rakers, Christin, Nguyen, Leslee T., Torgrimson, Megan R., Parico, Gian Carlo G., Tripathi, Sarvind, Zheng, Ning, Lander, Gabriel C., Hirota, Tsuyoshi, Tama, Florence, & Partch, Carrie L. Dynamics at the serine loop underlie differential affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing. United States. https://doi.org/10.7554/elife.55275
Fribourgh, Jennifer L., Srivastava, Ashutosh, Sandate, Colby R., Michael, Alicia K., Hsu, Peter L., Rakers, Christin, Nguyen, Leslee T., Torgrimson, Megan R., Parico, Gian Carlo G., Tripathi, Sarvind, Zheng, Ning, Lander, Gabriel C., Hirota, Tsuyoshi, Tama, Florence, and Partch, Carrie L. Wed .
"Dynamics at the serine loop underlie differential affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing". United States. https://doi.org/10.7554/elife.55275. https://www.osti.gov/servlets/purl/1644118.
@article{osti_1644118,
title = {Dynamics at the serine loop underlie differential affinity of cryptochromes for CLOCK:BMAL1 to control circadian timing},
author = {Fribourgh, Jennifer L. and Srivastava, Ashutosh and Sandate, Colby R. and Michael, Alicia K. and Hsu, Peter L. and Rakers, Christin and Nguyen, Leslee T. and Torgrimson, Megan R. and Parico, Gian Carlo G. and Tripathi, Sarvind and Zheng, Ning and Lander, Gabriel C. and Hirota, Tsuyoshi and Tama, Florence and Partch, Carrie L.},
abstractNote = {Mammalian circadian rhythms are generated by a transcription-based feedback loop in which CLOCK:BMAL1 drives transcription of its repressors (PER1/2, CRY1/2), which ultimately interact with CLOCK:BMAL1 to close the feedback loop with ~24 hr periodicity. Here we pinpoint a key difference between CRY1 and CRY2 that underlies their differential strengths as transcriptional repressors. Both cryptochromes bind the BMAL1 transactivation domain similarly to sequester it from coactivators and repress CLOCK:BMAL1 activity. However, we find that CRY1 is recruited with much higher affinity to the PAS domain core of CLOCK:BMAL1, allowing it to serve as a stronger repressor that lengthens circadian period. We discovered a dynamic serine-rich loop adjacent to the secondary pocket in the photolyase homology region (PHR) domain that regulates differential binding of cryptochromes to the PAS domain core of CLOCK:BMAL1. Notably, binding of the co-repressor PER2 remodels the serine loop of CRY2, making it more CRY1-like and enhancing its affinity for CLOCK:BMAL1.},
doi = {10.7554/elife.55275},
journal = {eLife},
number = 02, 2020,
volume = 9,
place = {United States},
year = {Wed Feb 26 00:00:00 EST 2020},
month = {Wed Feb 26 00:00:00 EST 2020}
}
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