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Title: Pore-scale view of microbial turnover: combining 14C imaging, μCT, and zymography after adding soluble carbon to soil pores of specific sizes

Abstract

The location of microorganisms and substrates within soil pore networks plays a crucial role for organic carbon (C) processing, its microbial utilization and turnover, and has direct consequences for C and nutrient cycling. An optimal approach to quantify responses to new C inputs from microorganisms residing in specific pores is the addition of new C to pores of target sizes in undisturbed soil cores. We used the matric potential approach to add 14C labeled glucose to small (< 40 μm, root free) or large (60-180 μm, potentially inhabited by roots) pores of undisturbed soil cores. Localization of glucose-derived C via 14C imaging was related to pore size distributions and connectivity, characterized via X-ray computed micro-tomography (μCT), and to β-glucosidase activity, characterized via zymography. After two-week incubations, 1.3 times more glucose was mineralized (14CO2) when it was added to the large pores, however more 14C remained in microbial biomass when glucose was added to the small pores. Consequently, while utilizing the same amounts of easily available C, the microorganisms localized in the large pores had faster turnover compared to microorganisms in small pores. Stronger associations between β-glucosidase activity and glucose-derived C were observed when glucose was added to the large pores.more » We conclude that 1) the matric potential approach allows placing, albeit not exactly, soluble substrates into pores of target diameter range, and 2) microorganisms localized in large pores respond to new C inputs with faster turnover, greater growth, and more intensive enzyme production compared to those inhabiting the small pores.« less

Authors:
ORCiD logo [1]; ORCiD logo [1];  [2];  [3];  [4]
+ Show Author Affiliations
  1. Michigan State Univ., East Lansing, MI (United States)
  2. Univ. of Kassel, Witzenhausen (Germany)
  3. Univ. of Göttingen (Germany)
  4. Univ. of Göttingen (Germany); Russian Academy of Sciences, Pushchino (Russia)
Publication Date:
Research Org.:
Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1637473
Alternate Identifier(s):
OSTI ID: 1646921
Grant/Contract Number:  
SC0018409; FC02-07ER64494
Resource Type:
Accepted Manuscript
Journal Name:
European Journal of Soil Science
Additional Journal Information:
Journal Volume: 72; Journal Issue: 2; Journal ID: ISSN 1351-0754
Publisher:
Wiley - British Society of Soil Science
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; microbial habitats; enzyme activity and localization; soil pore structure

Citation Formats

Kravchenko, A. N., Guber, A. K., Gunina, A., Dippold, M. A., and Kuzyakov, Y. Pore-scale view of microbial turnover: combining 14C imaging, μCT, and zymography after adding soluble carbon to soil pores of specific sizes. United States: N. p., 2020. Web. doi:10.1111/ejss.13001.
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Kravchenko, A. N., Guber, A. K., Gunina, A., Dippold, M. A., & Kuzyakov, Y. Pore-scale view of microbial turnover: combining 14C imaging, μCT, and zymography after adding soluble carbon to soil pores of specific sizes. United States. https://doi.org/10.1111/ejss.13001
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Kravchenko, A. N., Guber, A. K., Gunina, A., Dippold, M. A., and Kuzyakov, Y. Sun . "Pore-scale view of microbial turnover: combining 14C imaging, μCT, and zymography after adding soluble carbon to soil pores of specific sizes". United States. https://doi.org/10.1111/ejss.13001. https://www.osti.gov/servlets/purl/1637473.
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@article{osti_1637473,
title = {Pore-scale view of microbial turnover: combining 14C imaging, μCT, and zymography after adding soluble carbon to soil pores of specific sizes},
author = {Kravchenko, A. N. and Guber, A. K. and Gunina, A. and Dippold, M. A. and Kuzyakov, Y.},
abstractNote = {The location of microorganisms and substrates within soil pore networks plays a crucial role for organic carbon (C) processing, its microbial utilization and turnover, and has direct consequences for C and nutrient cycling. An optimal approach to quantify responses to new C inputs from microorganisms residing in specific pores is the addition of new C to pores of target sizes in undisturbed soil cores. We used the matric potential approach to add 14C labeled glucose to small (< 40 μm, root free) or large (60-180 μm, potentially inhabited by roots) pores of undisturbed soil cores. Localization of glucose-derived C via 14C imaging was related to pore size distributions and connectivity, characterized via X-ray computed micro-tomography (μCT), and to β-glucosidase activity, characterized via zymography. After two-week incubations, 1.3 times more glucose was mineralized (14CO2) when it was added to the large pores, however more 14C remained in microbial biomass when glucose was added to the small pores. Consequently, while utilizing the same amounts of easily available C, the microorganisms localized in the large pores had faster turnover compared to microorganisms in small pores. Stronger associations between β-glucosidase activity and glucose-derived C were observed when glucose was added to the large pores. We conclude that 1) the matric potential approach allows placing, albeit not exactly, soluble substrates into pores of target diameter range, and 2) microorganisms localized in large pores respond to new C inputs with faster turnover, greater growth, and more intensive enzyme production compared to those inhabiting the small pores.},
doi = {10.1111/ejss.13001},
journal = {European Journal of Soil Science},
number = 2,
volume = 72,
place = {United States},
year = {Sun May 24 00:00:00 EDT 2020},
month = {Sun May 24 00:00:00 EDT 2020}
}
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Publisher's Version of Record
https://doi.org/10.1111/ejss.13001
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