Arabidopsis myosin XI sub-domains homologous to the yeast myo2p organelle inheritance sub-domain target subcellular structures in plant cells

Sattarzadeh, Amirali; Schmelzer, Elmon; Hanson, Maureen R.

doi:10.3389/fpls.2013.00407

Title: Arabidopsis myosin XI sub-domains homologous to the yeast myo2p organelle inheritance sub-domain target subcellular structures in plant cells

Abstract

Myosin XI motor proteins transport plant organelles on the actin cytoskeleton. The Arabidopsis gene family that encodes myosin XI has 13 members, 12 of which have sub-domains within the tail region that are homologous to well-characterized cargo-binding domains in the yeast myosin V myo2p. Little is presently known about the cargo-binding domains of plant myosin XIs. Prior experiments in which most or all of the tail regions of myosin XIs have been fused to yellow fluorescent protein (YFP) and transiently expressed have often not resulted in fluorescent labeling of plant organelles. We identified 42 amino-acid regions within 12 Arabidopsis myosin XIs that are homologous to the yeast myo2p tail region known to be essential for vacuole and mitochondrial inheritance. A YFP fusion of the yeast region expressed in plants did not label tonoplasts or mitochondria. We investigated whether the homologous Arabidopsis regions, termed by us the “PAL” sub-domain, could associate with subcellular structures following transient expression of fusions with YFP in Nicotiana benthamiana. Seven YFP::PAL sub-domain fusions decorated Golgi and six were localized to mitochondria. In general, the myosin XI PAL sub-domains labeled organelles whose motility had previously been observed to be affected by mutagenesis or dominant negative assays withmore »« less

Authors:

Sattarzadeh, Amirali ^[1]; Schmelzer, Elmon ^[2]; Hanson, Maureen R. ^[3]

Cornell Univ., Ithaca, NY (United States). Dept. of Molecular Biology and Genetics; Max-Planck-Inst. for Plant Breeding Research, Cologne (Germany). Central Microscopy
Max-Planck-Inst. for Plant Breeding Research, Cologne (Germany). Central Microscopy
Cornell Univ., Ithaca, NY (United States). Dept. of Molecular Biology and Genetics

Publication Date:: Tue Oct 22 00:00:00 EDT 2013

Research Org.:: Cornell Univ., Ithaca, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

OSTI Identifier:: 1628247

Grant/Contract Number:: FG02-09ER16070; SC0002628

Resource Type:: Accepted Manuscript

Journal Name:: Frontiers in Plant Science

Additional Journal Information:: Journal Volume: 4; Journal ID: ISSN 1664-462X

Publisher:: Frontiers Research Foundation

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; plant sciences; yeast myo2p; myosin V; transient expression; Golgi; mitochondria; vacuole; confocal microscopy; Nicotiana benthamiana

Citation Formats


                    Sattarzadeh, Amirali, Schmelzer, Elmon, and Hanson, Maureen R. Arabidopsis myosin XI sub-domains homologous to the yeast myo2p organelle inheritance sub-domain target subcellular structures in plant cells.  United States: N. p., 2013. 
Web.  doi:10.3389/fpls.2013.00407.

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                    Sattarzadeh, Amirali, Schmelzer, Elmon, & Hanson, Maureen R. Arabidopsis myosin XI sub-domains homologous to the yeast myo2p organelle inheritance sub-domain target subcellular structures in plant cells.  United States.  https://doi.org/10.3389/fpls.2013.00407

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                    Sattarzadeh, Amirali, Schmelzer, Elmon, and Hanson, Maureen R. Tue .  
"Arabidopsis myosin XI sub-domains homologous to the yeast myo2p organelle inheritance sub-domain target subcellular structures in plant cells".  United States.  https://doi.org/10.3389/fpls.2013.00407.  https://www.osti.gov/servlets/purl/1628247.

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@article{osti_1628247,

  title        = {Arabidopsis myosin XI sub-domains homologous to the yeast myo2p organelle inheritance sub-domain target subcellular structures in plant cells},

  author       = {Sattarzadeh, Amirali and Schmelzer, Elmon and Hanson, Maureen R.},

  abstractNote = {Myosin XI motor proteins transport plant organelles on the actin cytoskeleton. The Arabidopsis gene family that encodes myosin XI has 13 members, 12 of which have sub-domains within the tail region that are homologous to well-characterized cargo-binding domains in the yeast myosin V myo2p. Little is presently known about the cargo-binding domains of plant myosin XIs. Prior experiments in which most or all of the tail regions of myosin XIs have been fused to yellow fluorescent protein (YFP) and transiently expressed have often not resulted in fluorescent labeling of plant organelles. We identified 42 amino-acid regions within 12 Arabidopsis myosin XIs that are homologous to the yeast myo2p tail region known to be essential for vacuole and mitochondrial inheritance. A YFP fusion of the yeast region expressed in plants did not label tonoplasts or mitochondria. We investigated whether the homologous Arabidopsis regions, termed by us the “PAL” sub-domain, could associate with subcellular structures following transient expression of fusions with YFP in Nicotiana benthamiana. Seven YFP::PAL sub-domain fusions decorated Golgi and six were localized to mitochondria. In general, the myosin XI PAL sub-domains labeled organelles whose motility had previously been observed to be affected by mutagenesis or dominant negative assays with the respective myosins. Simultaneous transient expression of the PAL sub-domains of myosin XI-H, XI-I, and XI-K resulted in inhibition of movement of mitochondria and Golgi.},

  doi          = {10.3389/fpls.2013.00407},

  journal      = {Frontiers in Plant Science},

  number       = ,

  volume       = 4,

  place        = {United States},

  year         = {Tue Oct 22 00:00:00 EDT 2013},

  month        = {Tue Oct 22 00:00:00 EDT 2013}

}

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Title: Arabidopsis myosin XI sub-domains homologous to the yeast myo2p organelle inheritance sub-domain target subcellular structures in plant cells

Abstract

Citation Formats

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