IncP-1ε Plasmids are Important Vectors of Antibiotic Resistance Genes in Agricultural Systems: Diversification Driven by Class 1 Integron Gene Cassettes
Abstract
The role of broad-host range IncP-1ε plasmids in the dissemination of antibiotic resistance in agricultural systems has not yet been investigated. These plasmids were detected in total DNA from all of 16 manure samples and in arable soil based on a novel 5 -nuclease assay for real-time PCR. A correlation between IncP-1ε plasmid abundance and antibiotic usage was revealed. In a soil microcosm experiment the abundance of IncP-1ε plasmids was significantly increased even 127 days after application of manure containing the antibiotic compound sulfadiazine, compared to soil receiving only manure, only sulfadiazine, or water. Fifty IncP-1ε plasmids that were captured in E. coli CV601gfp from bacterial communities of manure and arable soil were characterized by PCR and hybridization. All plasmids carried class 1 integrons with highly varying sizes of the gene cassette region and the sul1 gene. Three IncP-1ε plasmids captured from soil bacteria and one from manure were completely sequenced. The backbones were nearly identical to that of the previously described IncP-1ε plasmid pKJK5.The plasmids differed mainly in the composition of aTn402-like transposon carrying a class 1 integron with varying gene cassettes, IS1326, and in three of the plasmids the tetracycline resistance transposonTn1721 with various truncations. Diverse Betaand Gammaproteobacteriamore »
- Authors:
-
- Julius Kühn-Institut, Braunschweig (Germany ). Inst. for Epidemiology and Pathogen Diagnostics. Federal Research Centre for Cultivated Plants
- Universidad Adolfo Ibañez, Santiago (Chile). Dept. de Ciencias Biologicas
- Univ. of Idaho, Moscow, ID (United States). Inst. for Bioinformatics and Evolutionary Studies. Dept. of Biological Sciences
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1628050
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 3; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology; IncP-1ε plasmid; exogenous isolation; complete sequence; gene cassette; qPCR; arable soil; pig manure
Citation Formats
Heuer, Holger, Binh, Chu T. T., Jechalke, Sven, Kopmann, Christoph, Zimmerling, Ute, Krögerrecklenfort, Ellen, Ledger, Thomas, González, Bernardo, Top, Eva, and Smalla, Kornelia. IncP-1ε Plasmids are Important Vectors of Antibiotic Resistance Genes in Agricultural Systems: Diversification Driven by Class 1 Integron Gene Cassettes. United States: N. p., 2012.
Web. doi:10.3389/fmicb.2012.00002.
Heuer, Holger, Binh, Chu T. T., Jechalke, Sven, Kopmann, Christoph, Zimmerling, Ute, Krögerrecklenfort, Ellen, Ledger, Thomas, González, Bernardo, Top, Eva, & Smalla, Kornelia. IncP-1ε Plasmids are Important Vectors of Antibiotic Resistance Genes in Agricultural Systems: Diversification Driven by Class 1 Integron Gene Cassettes. United States. https://doi.org/10.3389/fmicb.2012.00002
Heuer, Holger, Binh, Chu T. T., Jechalke, Sven, Kopmann, Christoph, Zimmerling, Ute, Krögerrecklenfort, Ellen, Ledger, Thomas, González, Bernardo, Top, Eva, and Smalla, Kornelia. Sun .
"IncP-1ε Plasmids are Important Vectors of Antibiotic Resistance Genes in Agricultural Systems: Diversification Driven by Class 1 Integron Gene Cassettes". United States. https://doi.org/10.3389/fmicb.2012.00002. https://www.osti.gov/servlets/purl/1628050.
@article{osti_1628050,
title = {IncP-1ε Plasmids are Important Vectors of Antibiotic Resistance Genes in Agricultural Systems: Diversification Driven by Class 1 Integron Gene Cassettes},
author = {Heuer, Holger and Binh, Chu T. T. and Jechalke, Sven and Kopmann, Christoph and Zimmerling, Ute and Krögerrecklenfort, Ellen and Ledger, Thomas and González, Bernardo and Top, Eva and Smalla, Kornelia},
abstractNote = {The role of broad-host range IncP-1ε plasmids in the dissemination of antibiotic resistance in agricultural systems has not yet been investigated. These plasmids were detected in total DNA from all of 16 manure samples and in arable soil based on a novel 5 -nuclease assay for real-time PCR. A correlation between IncP-1ε plasmid abundance and antibiotic usage was revealed. In a soil microcosm experiment the abundance of IncP-1ε plasmids was significantly increased even 127 days after application of manure containing the antibiotic compound sulfadiazine, compared to soil receiving only manure, only sulfadiazine, or water. Fifty IncP-1ε plasmids that were captured in E. coli CV601gfp from bacterial communities of manure and arable soil were characterized by PCR and hybridization. All plasmids carried class 1 integrons with highly varying sizes of the gene cassette region and the sul1 gene. Three IncP-1ε plasmids captured from soil bacteria and one from manure were completely sequenced. The backbones were nearly identical to that of the previously described IncP-1ε plasmid pKJK5.The plasmids differed mainly in the composition of aTn402-like transposon carrying a class 1 integron with varying gene cassettes, IS1326, and in three of the plasmids the tetracycline resistance transposonTn1721 with various truncations. Diverse Betaand Gammaproteobacteria were revealed as hosts of one of the IncP-1ε plasmids in soil microcosms. Our data suggest that IncP-1ε plasmids are important vectors for horizontal transfer of antibiotic resistance in agricultural systems.},
doi = {10.3389/fmicb.2012.00002},
journal = {Frontiers in Microbiology},
number = ,
volume = 3,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 2012},
month = {Sun Jan 01 00:00:00 EST 2012}
}
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