Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B
Abstract
Staphylococcus aureus is implicated in many opportunistic bacterial infections around the world. Rising antibiotic resistance and few alternative methods of treatment are just two looming problems associated with clinical management of S. aureus. Among numerous virulence factors produced by S. aureus, staphylococcal enterotoxin (SE) B is a secreted protein that binds T-cell receptor and major histocompatibility complex class II, potentially causing toxic shock mediated by pathological activation of T cells. Recombinant monoclonal antibodies that target SEB and block receptor interactions can be of therapeutic value. The inhibitory and biophysical properties of ten human monoclonal antibodies, isolated from a recombinant library by panning against SEB vaccine (STEBVax), were examined as bivalent Fabs and native full-length IgG (Mab). The best performing Fabs had binding affinities equal to polyclonal IgG, low nanomolar IC50s against SEB in cell culture assays, and protected mice from SEB-induced toxic shock. The orthologous staphylococcal proteins, SEC1 and SEC2, as well as streptococcal pyrogenic exotoxin C were recognized by several Fabs. Four Fabs against SEB, with the lowest IC50s, were converted into native full-length Mabs. Although SEB-binding kinetics were identical between each Fab and respective Mab, a 250-fold greater inhibition of SEB-induced T-cell activation was observed with two Mabs.more »
- Authors:
-
- Medical Research Institute of Infectious Diseases, Frederick, MD (United States); Hood College, Frederick, MD (United States)
- Medical Research Institute of Infectious Diseases, Frederick, MD (United States); Wilson College, Chambersburg, PA (United States)
- Publication Date:
- Research Org.:
- Medical Research Institute of Infectious Diseases, Frederick, MD (United States); Hood College, Frederick, MD (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); Medical Research and Materiel Command; Joint Science and Technology Office
- OSTI Identifier:
- 1627427
- Grant/Contract Number:
- SC0014664
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 10; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; enzyme-linked immunoassays; antibodies; T cells; toxins; monoclonal antibodies; staphylococcus aureus; toxic shock syndrome; antibody therapy
Citation Formats
Larkin, Eileen A., Stiles, Bradley G., and Ulrich, Robert G. Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B. United States: N. p., 2010.
Web. doi:10.1371/journal.pone.0013253.
Larkin, Eileen A., Stiles, Bradley G., & Ulrich, Robert G. Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B. United States. https://doi.org/10.1371/journal.pone.0013253
Larkin, Eileen A., Stiles, Bradley G., and Ulrich, Robert G. Mon .
"Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B". United States. https://doi.org/10.1371/journal.pone.0013253. https://www.osti.gov/servlets/purl/1627427.
@article{osti_1627427,
title = {Inhibition of Toxic Shock by Human Monoclonal Antibodies against Staphylococcal Enterotoxin B},
author = {Larkin, Eileen A. and Stiles, Bradley G. and Ulrich, Robert G.},
abstractNote = {Staphylococcus aureus is implicated in many opportunistic bacterial infections around the world. Rising antibiotic resistance and few alternative methods of treatment are just two looming problems associated with clinical management of S. aureus. Among numerous virulence factors produced by S. aureus, staphylococcal enterotoxin (SE) B is a secreted protein that binds T-cell receptor and major histocompatibility complex class II, potentially causing toxic shock mediated by pathological activation of T cells. Recombinant monoclonal antibodies that target SEB and block receptor interactions can be of therapeutic value. The inhibitory and biophysical properties of ten human monoclonal antibodies, isolated from a recombinant library by panning against SEB vaccine (STEBVax), were examined as bivalent Fabs and native full-length IgG (Mab). The best performing Fabs had binding affinities equal to polyclonal IgG, low nanomolar IC50s against SEB in cell culture assays, and protected mice from SEB-induced toxic shock. The orthologous staphylococcal proteins, SEC1 and SEC2, as well as streptococcal pyrogenic exotoxin C were recognized by several Fabs. Four Fabs against SEB, with the lowest IC50s, were converted into native full-length Mabs. Although SEB-binding kinetics were identical between each Fab and respective Mab, a 250-fold greater inhibition of SEB-induced T-cell activation was observed with two Mabs. Results suggest that these human monoclonal antibodies possess high affinity, target specificity, and toxin neutralization qualities essential for any therapeutic agent.},
doi = {10.1371/journal.pone.0013253},
journal = {PLoS ONE},
number = 10,
volume = 5,
place = {United States},
year = {Mon Oct 11 00:00:00 EDT 2010},
month = {Mon Oct 11 00:00:00 EDT 2010}
}
Works referenced in this record:
Long-term effects of intravenous immunoglobulin in CIDP
journal, September 2007
- Vucic, S.; Black, K.; Baldassari, L. E.
- Clinical Neurophysiology, Vol. 118, Issue 9
Human Immunoglobulin G Recognizing Fibrinogen-Binding Surface Proteins Is Protective against both Staphylococcus aureus and Staphylococcus epidermidis Infections In Vivo
journal, February 2006
- Vernachio, John H.; Bayer, Arnold S.; Ames, Brenda
- Antimicrobial Agents and Chemotherapy, Vol. 50, Issue 2
Clinical Trial of Safety and Efficacy of IHN-A21 for the Prevention of Nosocomial Staphylococcal Bloodstream Infection in Premature Infants
journal, September 2007
- DeJonge, Mitchell; Burchfield, David; Bloom, Barry
- The Journal of Pediatrics, Vol. 151, Issue 3
Community-associated Methicillin-resistant Staphylococcus aureus Isolates and Healthcare-Associated Infections1
journal, February 2007
- Maree, Cynthia L.; Daum, Robert S.; Boyle-Vavra, Susan
- Emerging Infectious Diseases, Vol. 13, Issue 2
Potent neutralization of botulinum neurotoxin by recombinant oligoclonal antibody
journal, August 2002
- Nowakowski, A.; Wang, C.; Powers, D. B.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 17
egc , A Highly Prevalent Operon of Enterotoxin Gene, Forms a Putative Nursery of Superantigens in Staphylococcus aureus
journal, January 2001
- Jarraud, Sophie; Peyrat, Marie Alix; Lim, Annick
- The Journal of Immunology, Vol. 166, Issue 1
Clinical Usefulness of Intravenous Human Immunoglobulins in Invasive Group A Streptococcal Infections: Case Report and Review
journal, December 1995
- Lamothe, F.; D'Amico, P.; Ghosn, P.
- Clinical Infectious Diseases, Vol. 21, Issue 6
The staphylococcal enterotoxins and their relatives
journal, May 1990
- Marrack, P.; Kappler, J.
- Science, Vol. 248, Issue 4956
Differences in Potency of Intravenous Polyspecific Immunoglobulin G against Streptococcal and Staphylococcal Superantigens: Implications for Therapy of Toxic Shock Syndrome
journal, March 2004
- Darenberg, Jessica; Soderquist, Bo; Henriques Normark, Birgitta
- Clinical Infectious Diseases, Vol. 38, Issue 6
Staphylococcal enterotoxin B mutants (N23K and F44S): biological effects and vaccine potential in a mouse model
journal, February 1997
- Woody, Mary Alice; Krakauer, Teresa; Stiles, Bradley G.
- Vaccine, Vol. 15, Issue 2
Staphylococcal enterotoxins: A purging experience in review, Part I
journal, December 2005
- Stiles, Bradley G.; Krakauer, Teresa
- Clinical Microbiology Newsletter, Vol. 27, Issue 23
Staphylococcal enterotoxins A and B share a common structural motif for binding class II major histocompatibility complex molecules
journal, July 1995
- Ulrich, Robert G.; Bavari, Sina; Olson, Mark A.
- Nature Structural Biology, Vol. 2, Issue 7
Specificity, cross-reactivity and competition profile of monoclonal antibodies to staphylococcal enterotoxins B and C1 detected by indirect enzyme-linked immunosorbent assays
journal, April 1989
- Kienle, E.; Buschmann, H. G.
- Medical Microbiology and Immunology, Vol. 178, Issue 3
Costs of skin and skin structure infections due to S taphylococcus aureus : an analysis of managed- care claims
journal, August 2008
- Marton, Jeno P.; Jackel, Juliana L.; Carson, Robyn T.
- Current Medical Research and Opinion, Vol. 24, Issue 10
Development of engineered vaccines effective against structurally related bacterial superantigens
journal, November 1998
- Ulrich, R.
- Vaccine, Vol. 16, Issue 19
Neutralization of toxic shock syndrome toxin-1 by monoclonal antibodies in vitro and in vivo.
journal, January 1988
- Bonventre, P. F.; Thompson, M. R.; Adinolfi, L. E.
- Infection and Immunity, Vol. 56, Issue 1
Biological activity of toxic shock syndrome toxin 1 and a site-directed mutant, H135A, in a lipopolysaccharide-potentiated mouse lethality model.
journal, January 1995
- Stiles, B. G.; Krakauer, T.; Bonventre, P. F.
- Infection and immunity, Vol. 63, Issue 4
Correlation of Temperature and Toxicity in Murine Studies of Staphylococcal Enterotoxins and Toxic Shock Syndrome Toxin 1
journal, March 1999
- Stiles, Bradley G.; Campbell, Yvette G.; Castle, Robert M.
- Infection and Immunity, Vol. 67, Issue 3
Progress Towards Recombinant Anti-Infective Antibodies
journal, January 2009
- Pai, Jennifer; Sutherland, Jamie; Maynard, Jennifer
- Recent Patents on Anti-Infective Drug Discovery, Vol. 4, Issue 1
Influence of impaired T- and B-cell compartments on efficacy of IVIg in dysimmune neuropathies
journal, October 2007
- Matà, S.; Corzani, S.; Biagiotti, R.
- European Journal of Neurology, Vol. 14, Issue 10
Epidemiology of Methicillin‐Resistant Staphylococcus aureus
journal, June 2008
- Boucher, Helen W.; Corey, G. Ralph
- Clinical Infectious Diseases, Vol. 46, Issue S5
Use of intravenous immunoglobulin in the treatment of staphylococcal and streptococcal toxic shock syndromes and related illnesses☆☆☆
journal, October 2001
- Schlievert, Patrick M.
- Journal of Allergy and Clinical Immunology, Vol. 108, Issue 4
Clinical Usefulness of Intravenous Human Immunoglobulins in Invasive Group A Streptococcal Infections: Case Report and Review
journal, December 1995
- Lamothe, F.; D'Amico, P.; Ghosn, P.
- Clinical Infectious Diseases, Vol. 21, Issue 6
Pathogenesis of Methicillin‐Resistant Staphylococcus aureus Infection
journal, June 2008
- Gordon, Rachel J.; Lowy, Franklin D.
- Clinical Infectious Diseases, Vol. 46, Issue S5
Potent Neutralization of Staphylococcal Enterotoxin B by Synergistic Action of Chimeric Antibodies
journal, March 2010
- Tilahun, Mulualem E.; Rajagopalan, Govindarajan; Shah-Mahoney, Nalini
- Infection and Immunity, Vol. 78, Issue 6
Lack of Benefit of Intravenous Immune Globulin in a Murine Model of Group A Streptococcal Necrotizing Fasciitis
journal, January 2000
- Patel, Robin; Rouse, Mark S.; Florez, Monica V.
- The Journal of Infectious Diseases, Vol. 181, Issue 1
Immunotherapeutic strategies to combat staphylococcal infections
journal, August 2010
- Ohlsen, Knut; Lorenz, Udo
- International Journal of Medical Microbiology, Vol. 300, Issue 6
Vaccination and passive immunisation against Staphylococcus aureus
journal, November 2008
- Schaffer, Adam C.; Lee, Jean C.
- International Journal of Antimicrobial Agents, Vol. 32
A randomized prospective study on the use of 2 g-IVIG or 1 g-IVIG as therapy for Kawasaki disease
journal, November 2006
- Sakata, Koichi; Hamaoka, Kenji; Ozawa, Sei-ichiro
- European Journal of Pediatrics, Vol. 166, Issue 6
The Staphylococcal Enterotoxins and Their Relatives
journal, June 1990
- Marrack, P.; Kappler, J.
- Science, Vol. 248, Issue 4959
Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates
journal, September 2008
- Hu, Dong-Liang; Omoe, Katsuhiko; Inoue, Fumio
- Journal of Medical Microbiology, Vol. 57, Issue 9
Isolation of a Human Monoclonal Antibody with Strong Neutralizing Activity against Diphtheria Toxin
journal, June 2006
- Kakita, Mai; Takahashi, Tsuyoshi; Komiya, Takako
- Infection and Immunity, Vol. 74, Issue 6
Monoclonal antibodies to staphylococcal enterotoxins B and C: cross-reactivity and localization of epitopes on tryptic fragments.
journal, January 1984
- Thompson, N. E.; Ketterhagen, M. J.; Bergdoll, M. S.
- Infection and Immunity, Vol. 45, Issue 1
Gamma-globulin Inhibits Superantigen-induced Lymphocyte Proliferation and Cytokine Production
journal, January 2007
- Kato, Kumiko; Sakamoto, Tatsuo; Ito, Komei
- Allergology International, Vol. 56, Issue 4
Generation of protective immunity by inactivated recombinant staphylococcal enterotoxin B vaccine in nonhuman primates and identification of correlates of immunity
journal, July 2003
- Boles, James W.; M. Pitt, M. Louise; LeClaire, Ross D.
- Clinical Immunology, Vol. 108, Issue 1
Long-term effects of intravenous immunoglobulin in CIDP
journal, September 2007
- Vucic, S.; Black, K.; Baldassari, L. E.
- Clinical Neurophysiology, Vol. 118, Issue 9
egc , A Highly Prevalent Operon of Enterotoxin Gene, Forms a Putative Nursery of Superantigens in Staphylococcus aureus
journal, January 2001
- Jarraud, Sophie; Peyrat, Marie Alix; Lim, Annick
- The Journal of Immunology, Vol. 166, Issue 1
Characterization of novel type C staphylococcal enterotoxins: biological and evolutionary implications.
journal, January 1993
- Marr, J. C.; Lyon, J. D.; Roberson, J. R.
- Infection and Immunity, Vol. 61, Issue 10
Adjunctive Treatment of Streptococcal Toxic Shock Syndrome Using Intravenous Immunoglobulin: Case Report and Review
journal, January 1997
- Perez, Carlos M.; Kubak, Bernard M.; Cryer, Henry G.
- The American Journal of Medicine, Vol. 102, Issue 1
Cross‐Reactive Antibodies Prevent the Lethal Effects of Staphylococcus aureus Superantigens
journal, October 1999
- Bavari, Sina; Ulrich, Robert G.; LeClaire, Ross D.
- The Journal of Infectious Diseases, Vol. 180, Issue 4
Phase II, Randomized, Double-Blind, Multicenter Study Comparing the Safety and Pharmacokinetics of Tefibazumab to Placebo for Treatment of Staphylococcus aureus Bacteremia
journal, August 2006
- Weems, J. John; Steinberg, James P.; Filler, Scott
- Antimicrobial Agents and Chemotherapy, Vol. 50, Issue 8
Staphylococcal enterotoxins A and B share a common structural motif for binding class II major histocompatibility complex molecules
journal, July 1995
- Ulrich, Robert G.; Bavari, Sina; Olson, Mark A.
- Nature Structural Biology, Vol. 2, Issue 7
Invasive Methicillin-Resistant Staphylococcus aureus Infections in the United States
journal, January 2009
- Stockman, J. A.
- Yearbook of Pediatrics, Vol. 2009
Invasive Methicillin-Resistant Staphylococcus aureus Infections in the United States
journal, January 2008
- Kumar, A.
- Yearbook of Critical Care Medicine, Vol. 2008
Recurrent Nonmenstrual Toxic Shock Syndrome: Clinical Manifestations, Diagnosis, and Treatment
journal, May 2001
- Andrews, M. -M.; Parent, E. M.; Barry, M.
- Clinical Infectious Diseases, Vol. 32, Issue 10
Comparative prevalence of superantigenic toxin genes in meticillin-resistant and meticillin-susceptible Staphylococcus aureus isolates
journal, September 2008
- Hu, Dong-Liang; Omoe, Katsuhiko; Inoue, Fumio
- Journal of Medical Microbiology, Vol. 57, Issue 9
Pathogenesis of Methicillin‐Resistant Staphylococcus aureus Infection
journal, June 2008
- Gordon, Rachel J.; Lowy, Franklin D.
- Clinical Infectious Diseases, Vol. 46, Issue S5
Epidemiology of Methicillin‐Resistant Staphylococcus aureus
journal, June 2008
- Boucher, Helen W.; Corey, G. Ralph
- Clinical Infectious Diseases, Vol. 46, Issue S5
Structural and Functional Properties of Antibodies to the Superantigen TSST-1 and Their Relationship to Menstrual Toxic Shock Syndrome
journal, March 2007
- Kansal, Rita; Davis, Catherine; Hansmann, Melanie
- Journal of Clinical Immunology, Vol. 27, Issue 3
The staphylococcal enterotoxins and their relatives
journal, May 1990
- Marrack, P.; Kappler, J.
- Science, Vol. 248, Issue 4956
Differences in Potency of Intravenous Polyspecific Immunoglobulin G against Streptococcal and Staphylococcal Superantigens: Implications for Therapy of Toxic Shock Syndrome
journal, March 2004
- Darenberg, Jessica; Soderquist, Bo; Henriques Normark, Birgitta
- Clinical Infectious Diseases, Vol. 38, Issue 6
Prevalence of Toxic Shock Syndrome Toxin 1-Producing Staphylococcus aureus and the Presence of Antibodies to This Superantigen in Menstruating Women
journal, September 2005
- Parsonnet, J.; Hansmann, M. A.; Delaney, M. L.
- Journal of Clinical Microbiology, Vol. 43, Issue 9
Successful treatment of cytomegalovirus-associated hemophagocytic syndrome by intravenous immunoglobulins
journal, February 2008
- Hot, Arnaud; Madoux, Marie Hélène Girard; Viard, Jean P.
- American Journal of Hematology, Vol. 83, Issue 2
Use of intravenous immunoglobulin for the treatment of severe clostridium difficile colitis
journal, March 2007
- Hassoun, Ali; Ibrahim, Farrah
- The American Journal of Geriatric Pharmacotherapy, Vol. 5, Issue 1
Isolation of specific and common antibodies to staphylococcal enterotoxins B, C1, and C2.
journal, January 1980
- Lee, A. C.; Robbins, R. N.; Reiser, R. F.
- Infection and Immunity, Vol. 27, Issue 2
One in five mortality in non-menstrual toxic shock syndrome versus no mortality in menstrual cases in a balanced French series of 55 cases
journal, October 2007
- Descloux, E.; Perpoint, T.; Ferry, T.
- European Journal of Clinical Microbiology & Infectious Diseases, Vol. 27, Issue 1
One in five mortality in non-menstrual toxic shock syndrome versus no mortality in menstrual cases in a balanced French series of 55 cases
journal, October 2007
- Descloux, E.; Perpoint, T.; Ferry, T.
- European Journal of Clinical Microbiology & Infectious Diseases, Vol. 27, Issue 1
Proteomics-Based Identification of Anchorless Cell Wall Proteins as Vaccine Candidates against Staphylococcus aureus
journal, April 2009
- Glowalla, Eva; Tosetti, Bettina; Krönke, Martin
- Infection and Immunity, Vol. 77, Issue 7
Mucosal Vaccination with Recombinantly Attenuated Staphylococcal Enterotoxin B and Protection in a Murine Model
journal, April 2001
- Stiles, Bradley G.; Garza, Anthony R.; Ulrich, Robert G.
- Infection and Immunity, Vol. 69, Issue 4
Invasive Methicillin-Resistant <EMPH TYPE="ITAL">Staphylococcus aureus</EMPH> Infections in the United States
journal, October 2007
- Klevens, R. Monina
- JAMA, Vol. 298, Issue 15
Intravenous Immunoglobulin G Therapy in Streptococcal Toxic Shock Syndrome: A European Randomized, Double-Blind, Placebo-Controlled Trial
journal, August 2003
- Darenberg, J.; Ihendyane, N.; Sjolin, J.
- Clinical Infectious Diseases, Vol. 37, Issue 3
Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides
journal, February 2000
- Knappik, Achim; Ge, Liming; Honegger, Annemarie
- Journal of Molecular Biology, Vol. 296, Issue 1, p. 57-86
Use of intravenous immunoglobulin for the treatment of severe clostridium difficile colitis
journal, March 2007
- Hassoun, Ali; Ibrahim, Farrah
- The American Journal of Geriatric Pharmacotherapy, Vol. 5, Issue 1
Clinical and economic burden of antimicrobial resistance
journal, October 2008
- Maragakis, Lisa L.; Perencevich, Eli N.; Cosgrove, Sara E.
- Expert Review of Anti-infective Therapy, Vol. 6, Issue 5
Antibody for the prevention of neonatal noscocomial staphylococcal infection: a review of the literature
journal, January 2007
- Weisman, L. E.
- Archives de Pédiatrie, Vol. 14
Intravenous Immunoglobulin G Therapy in Streptococcal Toxic Shock Syndrome: A European Randomized, Double-Blind, Placebo-Controlled Trial
journal, August 2003
- Darenberg, J.; Ihendyane, N.; Sjolin, J.
- Clinical Infectious Diseases, Vol. 37, Issue 3
Lack of Benefit of Intravenous Immune Globulin in a Murine Model of Group A Streptococcal Necrotizing Fasciitis
journal, January 2000
- Patel, Robin; Rouse, Mark S.; Florez, Monica V.
- The Journal of Infectious Diseases, Vol. 181, Issue 1
Epidemiology of Methicillin-Resistant Staphylococcus aureus
journal, January 1999
- Fluckiger, U.; Widmer, A. F.
- Chemotherapy, Vol. 45, Issue 2
Staphylococcal enterotoxins: A purging experience in review, Part II
journal, December 2005
- Stiles, Bradley G.; Krakauer, Teresa
- Clinical Microbiology Newsletter, Vol. 27, Issue 24
Use of intravenous immunoglobulin in the treatment of staphylococcal and streptococcal toxic shock syndromes and related illnesses☆☆☆
journal, October 2001
- Schlievert, Patrick M.
- Journal of Allergy and Clinical Immunology, Vol. 108, Issue 4
Cross-neutralization of staphylococcal and streptococcal pyrogenic toxins by monoclonal and polyclonal antibodies.
journal, January 1988
- Bohach, G. A.; Hovde, C. J.; Handley, J. P.
- Infection and Immunity, Vol. 56, Issue 2
Costs of skin and skin structure infections due to S taphylococcus aureus : an analysis of managed- care claims
journal, August 2008
- Marton, Jeno P.; Jackel, Juliana L.; Carson, Robyn T.
- Current Medical Research and Opinion, Vol. 24, Issue 10
Toxic Shock Syndrome in a Neonate
journal, January 2007
- Powell, Christine; Bubb, Samantha; Clark, Julia
- Pediatric Infectious Disease Journal, Vol. 26, Issue 8
Staphylococcal enterotoxin A and toxic shock syndrome toxin compete with CD4 for human major histocompatibility complex class II binding.
journal, January 1995
- Bavari, S.; Ulrich, R. G.
- Infection and immunity, Vol. 63, Issue 2
Staphylococcal Vaccines and Immunotherapies
journal, March 2009
- Schaffer, Adam C.; Lee, Jean C.
- Infectious Disease Clinics of North America, Vol. 23, Issue 1
Successful treatment of cytomegalovirus-associated hemophagocytic syndrome by intravenous immunoglobulins
journal, February 2008
- Hot, Arnaud; Madoux, Marie Hélène Girard; Viard, Jean P.
- American Journal of Hematology, Vol. 83, Issue 2
Protection against Bacterial Superantigen Staphylococcal Enterotoxin B by Passive Vaccination
journal, May 2002
- LeClaire, R. D.
- Infection and Immunity, Vol. 70, Issue 5
Neutralization of staphylococcal exotoxins in vitro by human-origin intravenous immunoglobulin
journal, January 2007
- Yanagisawa, Chie; Hanaki, Hideaki; Natae, Taiji
- Journal of Infection and Chemotherapy, Vol. 13, Issue 6
The staphylococcal enterotoxins and their relatives
journal, June 1990
- Marrack, P.; Kappler, J.
- Science, Vol. 248, Issue 4959
Structural and Functional Properties of Antibodies to the Superantigen TSST-1 and Their Relationship to Menstrual Toxic Shock Syndrome
journal, March 2007
- Kansal, Rita; Davis, Catherine; Hansmann, Melanie
- Journal of Clinical Immunology, Vol. 27, Issue 3
Intravenous immunoglobulin contains specific antibodies inhibitory to activation of T cells by staphylococcal toxin superantigens [see comment]
journal, February 1993
- Takei, S.; Arora, Y. K.; Walker, S. M.
- Journal of Clinical Investigation, Vol. 91, Issue 2
Immunotherapy with human monoclonal antibodies. Fragment A specificity of polyclonal and monoclonal antibodies is crucial for full protection against tetanus toxin.
journal, July 1993
- Lang, A. B.; Cryz, S. J.; Schürch, U.
- The Journal of Immunology, Vol. 151, Issue 1
On the Cross-Reactivity of Staphylococcal Enterotoxins A, B, and C
journal, January 1978
- Spero, Leonard; Morlock, Beverly A.; Metzger, Joseph F.
- The Journal of Immunology, Vol. 120, Issue 1
Works referencing / citing this record:
Chimeric Anti-Staphylococcal Enterotoxin B Antibodies and Lovastatin Act Synergistically to Provide In Vivo Protection against Lethal Doses of SEB
journal, November 2011
- Tilahun, Mulualem E.; Kwan, Alan; Natarajan, Kannan
- PLoS ONE, Vol. 6, Issue 11
Monoclonal Antibodies and Toxins—A Perspective on Function and Isotype
journal, June 2012
- Chow, Siu-Kei; Casadevall, Arturo
- Toxins, Vol. 4, Issue 6
Effective Treatment of Staphylococcal Enterotoxin B Aerosol Intoxication in Rhesus Macaques by Using Two Parenterally Administered High-Affinity Monoclonal Antibodies
journal, May 2019
- Verreault, Daniel; Ennis, Jane; Whaley, Kevin
- Antimicrobial Agents and Chemotherapy, Vol. 63, Issue 5
Therapeutic Treatment of Aerosolized Staphylococcal Enterotoxin B in Nonhuman Primates with two Monoclonal Antibodies
posted_content, January 2018
- Verreault, Daniel; Ennis, Jane; Whaley, Kevin
- BioRxiv
The Road to Toxin-Targeted Therapeutic Antibodies
journal, August 2014
- Kozel, Thomas R.
- mBio, Vol. 5, Issue 4
Toxic Shock Syndrome Toxin-1-Mediated Toxicity Inhibited by Neutralizing Antibodies Late in the Course of Continual in Vivo and in Vitro Exposure
journal, May 2014
- Stich, Norbert; Model, Nina; Samstag, Aysen
- Toxins, Vol. 6, Issue 6
Effective Treatment of Staphylococcal Enterotoxin B Aerosol Intoxication in Rhesus Macaques by Using Two Parenterally Administered High-Affinity Monoclonal Antibodies
journal, May 2019
- Verreault, Daniel; Ennis, Jane; Whaley, Kevin
- Antimicrobial Agents and Chemotherapy, Vol. 63, Issue 5
An easy and efficient permeabilization protocol for in vivo enzyme activity assays in cyanobacteria
journal, November 2016
- Rasmussen, Randi Engelberth; Erstad, Simon Matthé; Ramos-Martinez, Erick Miguel
- Microbial Cell Factories, Vol. 15, Issue 1
Sepsis: mechanisms of bacterial injury to the patient
journal, February 2019
- Minasyan, Hayk
- Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, Vol. 27, Issue 1
Synthetic Human Monoclonal Antibodies toward Staphylococcal Enterotoxin B (SEB) Protective against Toxic Shock Syndrome
journal, May 2012
- Karauzum, Hatice; Chen, Gang; Abaandou, Laura
- Journal of Biological Chemistry, Vol. 287, Issue 30
Monoclonal Antibodies and Toxins—A Perspective on Function and Isotype
journal, June 2012
- Chow, Siu-Kei; Casadevall, Arturo
- Toxins, Vol. 4, Issue 6
Sepsis: mechanisms of bacterial injury to the patient
journal, February 2019
- Minasyan, Hayk
- Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, Vol. 27, Issue 1
PI3K/Akt/mTOR, a Pathway Less Recognized for Staphylococcal Superantigen-Induced Toxicity
journal, November 2012
- Krakauer, Teresa
- Toxins, Vol. 4, Issue 11
The staphylococcal enterotoxin (SE) family: SEB and siblings
journal, November 2013
- Krakauer, Teresa; Stiles, Bradley G.
- Virulence, Vol. 4, Issue 8
Rapid detection of Bacillus ionophore cereulide in food products
journal, April 2019
- Ducrest, P. J.; Pfammatter, S.; Stephan, D.
- Scientific Reports, Vol. 9, Issue 1
Update on Staphylococcal Superantigen-Induced Signaling Pathways and Therapeutic Interventions
journal, September 2013
- Krakauer, Teresa
- Toxins, Vol. 5, Issue 9
Mechanisms Mediating Enhanced Neutralization Efficacy of Staphylococcal Enterotoxin B by Combinations of Monoclonal Antibodies
journal, March 2015
- Dutta, Kaushik; Varshney, Avanish K.; Franklin, Matthew C.
- Journal of Biological Chemistry, Vol. 290, Issue 11
Antibodies for biodefense
journal, November 2011
- Froude, Jeffrey W.; Stiles, Bradley G.; Pelat, Thibaut
- mAbs, Vol. 3, Issue 6
Isotype Switching Increases Efficacy of Antibody Protection against Staphylococcal Enterotoxin B-Induced Lethal Shock and Staphylococcus aureus Sepsis in Mice
journal, June 2014
- Varshney, Avanish K.; Wang, Xiaobo; Aguilar, Jorge L.
- mBio, Vol. 5, Issue 3
PI3K/Akt/mTOR, a Pathway Less Recognized for Staphylococcal Superantigen-Induced Toxicity
journal, November 2012
- Krakauer, Teresa
- Toxins, Vol. 4, Issue 11
FDA-approved immunosuppressants targeting staphylococcal superantigens: mechanisms and insights
journal, May 2017
- Krakauer, Teresa
- ImmunoTargets and Therapy, Vol. Volume 6
Mesenchymal stromal (stem) cells suppress pro-inflammatory cytokine production but fail to improve survival in experimental staphylococcal toxic shock syndrome
journal, January 2014
- Kim, Hani; Darwish, Ilyse; Monroy, Maria-Fernanda
- BMC Immunology, Vol. 15, Issue 1
Update on Staphylococcal Superantigen-Induced Signaling Pathways and Therapeutic Interventions
journal, September 2013
- Krakauer, Teresa
- Toxins, Vol. 5, Issue 9
Review of the Inhibition of Biological Activities of Food-Related Selected Toxins by Natural Compounds
journal, April 2013
- Friedman, Mendel; Rasooly, Reuven
- Toxins, Vol. 5, Issue 4
Staphylococcal Superantigens: Pyrogenic Toxins Induce Toxic Shock
journal, March 2019
- Krakauer, Teresa
- Toxins, Vol. 11, Issue 3
Evaluation of a novel type of imaging probe based on a recombinant bivalent mini-antibody construct for detection of CD44v6-expressing squamous cell carcinoma
journal, December 2015
- Haylock, Anna-Karin; Spiegelberg, Diana; Mortensen, Anja C.
- International Journal of Oncology, Vol. 48, Issue 2
The staphylococcal enterotoxin (SE) family: SEB and siblings
journal, November 2013
- Krakauer, Teresa; Stiles, Bradley G.
- Virulence, Vol. 4, Issue 8
Staphylococcal Superantigens: Pyrogenic Toxins Induce Toxic Shock
journal, March 2019
- Krakauer, Teresa
- Toxins, Vol. 11, Issue 3
Engineered pH-dependent recycling antibodies enhance elimination of Staphylococcal enterotoxin B superantigen in mice
journal, December 2018
- Kroetsch, Andrew; Qiao, Chunxia; Heavey, Mairead
- mAbs, Vol. 11, Issue 2