Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification
Abstract
Non-productive binding of enzymes to lignin is thought to impede the saccharification efficiency of pretreated lignocellulosic biomass to fermentable sugars. Due to a lack of suitable analytical techniques that track binding of individual enzymes within complex protein mixtures and the difficulty in distinguishing the contribution of productive (binding to specific glycans) versus non-productive (binding to lignin) binding of cellulases to lignocellulose, there is currently a poor understanding of individual enzyme adsorption to lignin during the time course of pretreated biomass saccharification.
- Authors:
-
- Michigan State University, East Lansing, MI (United States), Dept. of Chemical Engineering and Materials Science; Michigan State University, East Lansing, MI (United States), Great Lakes Bioenergy Research Center (GLBRC); Biomass Conversion Research Lab (BCRL) East Lansing, MI (United States)
- Michigan State University, East Lansing, MI (United States), Dept. of Chemical Engineering and Materials Science
- Michigan State University, East Lansing, MI (United States), Dept. of Chemical Engineering and Materials Science; Michigan State University, East Lansing, MI (United States), Dept. of Biosystems and Agricultural Engineering
- Michigan State University, East Lansing, MI (United States), Dept. of Chemical Engineering and Materials Science; Michigan State University, East Lansing, MI (United States), Great Lakes Bioenergy Research Center (GLBRC); Biomass Conversion Research Lab (BCRL) East Lansing, MI (United States): The State University of New Jersey, Piscataway, NJ, (United States), Dept. of Chemical & Biochemical Engineering Rutgers,
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1626961
- Grant/Contract Number:
- FC02-07ER64494
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Biotechnology & Applied Microbiology; Energy & Fuels
Citation Formats
Gao, Dahai, Haarmeyer, Carolyn, Balan, Venkatesh, Whitehead, Timothy A., Dale, Bruce E., and Chundawat, Shishir PS. Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification. United States: N. p., 2014.
Web. doi:10.1186/s13068-014-0175-x.
Gao, Dahai, Haarmeyer, Carolyn, Balan, Venkatesh, Whitehead, Timothy A., Dale, Bruce E., & Chundawat, Shishir PS. Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification. United States. https://doi.org/10.1186/s13068-014-0175-x
Gao, Dahai, Haarmeyer, Carolyn, Balan, Venkatesh, Whitehead, Timothy A., Dale, Bruce E., and Chundawat, Shishir PS. Mon .
"Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification". United States. https://doi.org/10.1186/s13068-014-0175-x. https://www.osti.gov/servlets/purl/1626961.
@article{osti_1626961,
title = {Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification},
author = {Gao, Dahai and Haarmeyer, Carolyn and Balan, Venkatesh and Whitehead, Timothy A. and Dale, Bruce E. and Chundawat, Shishir PS},
abstractNote = {Non-productive binding of enzymes to lignin is thought to impede the saccharification efficiency of pretreated lignocellulosic biomass to fermentable sugars. Due to a lack of suitable analytical techniques that track binding of individual enzymes within complex protein mixtures and the difficulty in distinguishing the contribution of productive (binding to specific glycans) versus non-productive (binding to lignin) binding of cellulases to lignocellulose, there is currently a poor understanding of individual enzyme adsorption to lignin during the time course of pretreated biomass saccharification.},
doi = {10.1186/s13068-014-0175-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 7,
place = {United States},
year = {Mon Dec 01 00:00:00 EST 2014},
month = {Mon Dec 01 00:00:00 EST 2014}
}
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