Analytical method for the determination of organic acids in dilute acid pretreated biomass hydrolysate by liquid chromatography-time-of-flight mass spectrometry
Abstract
For the development of lignocellulosic biofuels a common strategy to release hemicellulosic sugars and enhance the enzymatic digestibility of cellulose is the heat pretreatment of biomass with dilute acid. During this process, fermentation inhibitors such as 5-hydroxymethylfurfural, furfural, phenolics, and organic acids are formed and released into the so-called hydrolysate. The phenolic inhibitors have been studied fairly extensively, but fewer studies have focused on the analysis of the organic acids profile. For this purpose, a simple and fast liquid chromatography/mass spectrometry (LC/MS) method for the analysis of organic acids in the hydrolysate has been developed using an ion exchange column based on a polystyrene-divinylbenzene polymer frequently used in biofuel research. The application of the LC/MS method to a hydrolysate from Miscanthus has been evaluated.
- Authors:
-
- Univ. of California, Berkeley, CA (United States). Energy Biosciences Institute
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1626960
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Biotechnology & Applied Microbiology; Energy & Fuels
Citation Formats
Ibáñez, Ana B., and Bauer, Stefan. Analytical method for the determination of organic acids in dilute acid pretreated biomass hydrolysate by liquid chromatography-time-of-flight mass spectrometry. United States: N. p., 2014.
Web. doi:10.1186/s13068-014-0145-3.
Ibáñez, Ana B., & Bauer, Stefan. Analytical method for the determination of organic acids in dilute acid pretreated biomass hydrolysate by liquid chromatography-time-of-flight mass spectrometry. United States. https://doi.org/10.1186/s13068-014-0145-3
Ibáñez, Ana B., and Bauer, Stefan. Wed .
"Analytical method for the determination of organic acids in dilute acid pretreated biomass hydrolysate by liquid chromatography-time-of-flight mass spectrometry". United States. https://doi.org/10.1186/s13068-014-0145-3. https://www.osti.gov/servlets/purl/1626960.
@article{osti_1626960,
title = {Analytical method for the determination of organic acids in dilute acid pretreated biomass hydrolysate by liquid chromatography-time-of-flight mass spectrometry},
author = {Ibáñez, Ana B. and Bauer, Stefan},
abstractNote = {For the development of lignocellulosic biofuels a common strategy to release hemicellulosic sugars and enhance the enzymatic digestibility of cellulose is the heat pretreatment of biomass with dilute acid. During this process, fermentation inhibitors such as 5-hydroxymethylfurfural, furfural, phenolics, and organic acids are formed and released into the so-called hydrolysate. The phenolic inhibitors have been studied fairly extensively, but fewer studies have focused on the analysis of the organic acids profile. For this purpose, a simple and fast liquid chromatography/mass spectrometry (LC/MS) method for the analysis of organic acids in the hydrolysate has been developed using an ion exchange column based on a polystyrene-divinylbenzene polymer frequently used in biofuel research. The application of the LC/MS method to a hydrolysate from Miscanthus has been evaluated.},
doi = {10.1186/s13068-014-0145-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 7,
place = {United States},
year = {Wed Oct 08 00:00:00 EDT 2014},
month = {Wed Oct 08 00:00:00 EDT 2014}
}
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Works referencing / citing this record:
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- Bauer, Stefan; Ibáñez, Ana B.
- BMC Biotechnology, Vol. 15, Issue 1