Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens
Abstract
It has been proposed that Geobacter sulfurreducens requires conductive pili for long-range electron transport to Fe(III) oxides and for high-density current production in microbial fuel cells. In order to investigate this further, we constructed a strain of G. sulfurreducens, designated Aro-5, which produced pili with diminished conductivity. This was accomplished by modifying the amino acid sequence of PilA, the structural pilin protein. An alanine was substituted for each of the five aromatic amino acids in the carboxyl terminus of PilA, the region in which G. sulfurreducens PilA differs most significantly from the PilAs of microorganisms incapable of long-range extracellular electron transport. Strain Aro-5 produced pili that were properly decorated with the multiheme c-type cytochrome OmcS, which is essential for Fe(III) oxide reduction. However, pili preparations of the Aro-5 strain had greatly diminished conductivity and Aro-5 cultures were severely limited in their capacity to reduce Fe(III) compared to the control strain. Current production of the Aro-5 strain, with a graphite anode serving as the electron acceptor, was less than 10% of that of the control strain. The conductivity of the Aro-5 biofilms was 10-fold lower than the control strain’s. These results demonstrate that the pili of G. sulfurreducens must be conductivemore »
- Authors:
-
- Univ. of Massachusetts, Amherst, MA (United States). Dept. of Microbiology; College of the Holy Cross, Worcester, MA (United States). Dept. of Biology
- Univ. of Massachusetts, Amherst, MA (United States). Dept. of Microbiology
- Publication Date:
- Research Org.:
- Univ. of Massachusetts, Amherst, MA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1626085
- Grant/Contract Number:
- SC0004114
- Resource Type:
- Accepted Manuscript
- Journal Name:
- mBio (Online)
- Additional Journal Information:
- Journal Name: mBio (Online); Journal Volume: 4; Journal Issue: 2; Journal ID: ISSN 2150-7511
- Publisher:
- American Society for Microbiology (ASM)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology
Citation Formats
Vargas, Madeline, Malvankar, Nikhil S., Tremblay, Pier-Luc, Leang, Ching, Smith, Jessica A., Patel, Pranav, Synoeyenbos-West, Oona, Nevin, Kelly P., and Lovley, Derek R. Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens. United States: N. p., 2013.
Web. doi:10.1128/mbio.00105-13.
Vargas, Madeline, Malvankar, Nikhil S., Tremblay, Pier-Luc, Leang, Ching, Smith, Jessica A., Patel, Pranav, Synoeyenbos-West, Oona, Nevin, Kelly P., & Lovley, Derek R. Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens. United States. https://doi.org/10.1128/mbio.00105-13
Vargas, Madeline, Malvankar, Nikhil S., Tremblay, Pier-Luc, Leang, Ching, Smith, Jessica A., Patel, Pranav, Synoeyenbos-West, Oona, Nevin, Kelly P., and Lovley, Derek R. Tue .
"Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens". United States. https://doi.org/10.1128/mbio.00105-13. https://www.osti.gov/servlets/purl/1626085.
@article{osti_1626085,
title = {Aromatic Amino Acids Required for Pili Conductivity and Long-Range Extracellular Electron Transport in Geobacter sulfurreducens},
author = {Vargas, Madeline and Malvankar, Nikhil S. and Tremblay, Pier-Luc and Leang, Ching and Smith, Jessica A. and Patel, Pranav and Synoeyenbos-West, Oona and Nevin, Kelly P. and Lovley, Derek R.},
abstractNote = {It has been proposed that Geobacter sulfurreducens requires conductive pili for long-range electron transport to Fe(III) oxides and for high-density current production in microbial fuel cells. In order to investigate this further, we constructed a strain of G. sulfurreducens, designated Aro-5, which produced pili with diminished conductivity. This was accomplished by modifying the amino acid sequence of PilA, the structural pilin protein. An alanine was substituted for each of the five aromatic amino acids in the carboxyl terminus of PilA, the region in which G. sulfurreducens PilA differs most significantly from the PilAs of microorganisms incapable of long-range extracellular electron transport. Strain Aro-5 produced pili that were properly decorated with the multiheme c-type cytochrome OmcS, which is essential for Fe(III) oxide reduction. However, pili preparations of the Aro-5 strain had greatly diminished conductivity and Aro-5 cultures were severely limited in their capacity to reduce Fe(III) compared to the control strain. Current production of the Aro-5 strain, with a graphite anode serving as the electron acceptor, was less than 10% of that of the control strain. The conductivity of the Aro-5 biofilms was 10-fold lower than the control strain’s. These results demonstrate that the pili of G. sulfurreducens must be conductive in order for the cells to be effective in extracellular long-range electron transport.},
doi = {10.1128/mbio.00105-13},
journal = {mBio (Online)},
number = 2,
volume = 4,
place = {United States},
year = {Tue Mar 12 00:00:00 EDT 2013},
month = {Tue Mar 12 00:00:00 EDT 2013}
}
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e-Biologics: Fabrication of Sustainable Electronics with “Green” Biological Materials
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Expressing the Geobacter metallireducens PilA in Geobacter sulfurreducens Yields Pili with Exceptional Conductivity
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- The University of North Carolina at Chapel Hill University Libraries
The Low Conductivity of Geobacter uraniireducens Pili Suggests a Diversity of Extracellular Electron Transfer Mechanisms in the Genus Geobacter
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Plugging in or going wireless: strategies for interspecies electron transfer
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Investigating microbial activities of electrode-associated microorganisms in real-time
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Electrifying microbes for the production of chemicals
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Geobacter Protein Nanowires
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