Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates
Abstract
Cell-derived extracellular matrix (ECM) provides a niche to promote osteogenic differentiation, cell adhesion, survival, and trophic factor secretion. To determine whether osteogenic preconditioning would improve the bone-forming potential of unfractionated bone marrow aspirate (BMA), we perfused cells on ECM-coated scaffolds to generate naïve and preconditioned constructs, respectively. The composition of cells selected from BMA was distinct on each scaffold. Naïve constructs exhibited robust proangiogenic potential in vitro, while preconditioned scaffolds contained more mesenchymal stem/stromal cells (MSCs) and endothelial cells (ECs) and exhibited an osteogenic phenotype. Upon implantation into an orthotopic calvarial defect, BMA-derived ECs were present in vessels in preconditioned implants, resulting in robust perfusion and greater vessel density over the first 14 days compared to naïve implants. After 10 weeks, human ECs and differentiated MSCs were detected in de novo tissues derived from naïve and preconditioned scaffolds. These results demonstrate that bioreactor-based preconditioning augments the bone-forming potential of BMA.
- Authors:
-
- Univ. of California, Davis, CA (United States). Dept. of Biomedical Engineering
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Physical and Life Sciences
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Physical and Life Sciences
- Univ. of California, Davis, CA (United States). Department of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine
- Univ. of California, Davis, CA (United States). Dept. of Biomedical Engineering; UC Davis Health, Sacramento, California (United States). Department of Orthopaedic Surgery, School of Medicine
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1626036
- Alternate Identifier(s):
- OSTI ID: 1755844
- Report Number(s):
- LLNL-JRNL-789381
Journal ID: ISSN 2375-2548
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Science Advances
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 7; Journal ID: ISSN 2375-2548
- Publisher:
- AAAS
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; extracellular matrix; bone marrow aspirate; bioreactor; osteogenesis; vascularization
Citation Formats
Harvestine, J. N., Gonzalez-Fernandez, T., Sebastian, A., Hum, N. R., Genetos, D. C., Loots, G. G., and Leach, J. K. Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates. United States: N. p., 2020.
Web. doi:10.1126/sciadv.aay2387.
Harvestine, J. N., Gonzalez-Fernandez, T., Sebastian, A., Hum, N. R., Genetos, D. C., Loots, G. G., & Leach, J. K. Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates. United States. https://doi.org/10.1126/sciadv.aay2387
Harvestine, J. N., Gonzalez-Fernandez, T., Sebastian, A., Hum, N. R., Genetos, D. C., Loots, G. G., and Leach, J. K. Wed .
"Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates". United States. https://doi.org/10.1126/sciadv.aay2387. https://www.osti.gov/servlets/purl/1626036.
@article{osti_1626036,
title = {Osteogenic preconditioning in perfusion bioreactors improves vascularization and bone formation by human bone marrow aspirates},
author = {Harvestine, J. N. and Gonzalez-Fernandez, T. and Sebastian, A. and Hum, N. R. and Genetos, D. C. and Loots, G. G. and Leach, J. K.},
abstractNote = {Cell-derived extracellular matrix (ECM) provides a niche to promote osteogenic differentiation, cell adhesion, survival, and trophic factor secretion. To determine whether osteogenic preconditioning would improve the bone-forming potential of unfractionated bone marrow aspirate (BMA), we perfused cells on ECM-coated scaffolds to generate naïve and preconditioned constructs, respectively. The composition of cells selected from BMA was distinct on each scaffold. Naïve constructs exhibited robust proangiogenic potential in vitro, while preconditioned scaffolds contained more mesenchymal stem/stromal cells (MSCs) and endothelial cells (ECs) and exhibited an osteogenic phenotype. Upon implantation into an orthotopic calvarial defect, BMA-derived ECs were present in vessels in preconditioned implants, resulting in robust perfusion and greater vessel density over the first 14 days compared to naïve implants. After 10 weeks, human ECs and differentiated MSCs were detected in de novo tissues derived from naïve and preconditioned scaffolds. These results demonstrate that bioreactor-based preconditioning augments the bone-forming potential of BMA.},
doi = {10.1126/sciadv.aay2387},
journal = {Science Advances},
number = 7,
volume = 6,
place = {United States},
year = {Wed Feb 12 00:00:00 EST 2020},
month = {Wed Feb 12 00:00:00 EST 2020}
}
Figures / Tables:
Works referenced in this record:
In vitro biomimetic engineering of a human hematopoietic niche with functional properties
journal, June 2018
- Bourgine, Paul E.; Klein, Thibaut; Paczulla, Anna M.
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 25
Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner
journal, September 2002
- Bancroft, G. N.; Sikavitsas, V. I.; van den Dolder, J.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 20, p. 12600-12605
Quantifying Mesenchymal Stem Cells in the Mononuclear Cell Fraction of Bone Marrow Samples Obtained for Cell Therapy
journal, January 2013
- Alvarez-Viejo, M.; Menendez-Menendez, Y.; Blanco-Gelaz, M. A.
- Transplantation Proceedings, Vol. 45, Issue 1
Dlx5, a Positive Regulator of Osteoblastogenesis, is Essential for Osteoblast-Osteoclast Coupling
journal, September 2008
- Samee, Nadeem; Geoffroy, Valerie; Marty, Caroline
- The American Journal of Pathology, Vol. 173, Issue 3
Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity
journal, September 2003
- Wendt, D.; Marsano, A.; Jakob, M.
- Biotechnology and Bioengineering, Vol. 84, Issue 2
The role of ECM proteins and protein fragments in guiding cell behavior in regenerative medicine
journal, June 2011
- Barker, Thomas H.
- Biomaterials, Vol. 32, Issue 18
Flow rates in perfusion bioreactors to maximise mineralisation in bone tissue engineering in vitro
journal, October 2018
- Zhao, Feihu; van Rietbergen, Bert; Ito, Keita
- Journal of Biomechanics, Vol. 79
Multiple levels of steroid hormone-dependent control of osteocalcin during osteoblast differentiation: Glucocorticoid regulation of basal and vitamin D stimulated gene expression
journal, May 1998
- Shalhoub, Victoria; Aslam, Fauzia; Breen, Ellen
- Journal of Cellular Biochemistry, Vol. 69, Issue 2
Cartilage to bone transformation during fracture healing is coordinated by the invading vasculature and induction of the core pluripotency genes
journal, January 2017
- Hu, Diane P.; Ferro, Federico; Yang, Frank
- Development, Vol. 144, Issue 2
Differentiation-Dependent Secretion of Proangiogenic Factors by Mesenchymal Stem Cells
journal, April 2012
- Hoch, Allison I.; Binder, Bernard Y.; Genetos, Damian C.
- PLoS ONE, Vol. 7, Issue 4
Protein and Mineral Composition of Osteogenic Extracellular Matrix Constructs Generated with a Flow Perfusion Bioreactor
journal, December 2011
- Thibault, Richard A.; Mikos, Antonios G.; Kasper, F. Kurtis
- Biomacromolecules, Vol. 12, Issue 12
Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces
journal, December 2003
- Sikavitsas, V. I.; Bancroft, G. N.; Holtorf, H. L.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 25
Stem Cell-Derived Endochondral Cartilage Stimulates Bone Healing by Tissue Transformation: CARTILAGE TRANSFORMATION STIMULATES BONE HEALING
journal, April 2014
- Bahney, Chelsea S.; Hu, Diane P.; Taylor, Aaron J.
- Journal of Bone and Mineral Research, Vol. 29, Issue 5
Establishment of an Early Vascular Network Promotes the Formation of Ectopic Bone
journal, February 2016
- Eman, Rhandy M.; Meijer, Henriette A. W.; Öner, F. Cumhur
- Tissue Engineering Part A, Vol. 22, Issue 3-4
Expansion of Bone Marrow Mesenchymal Stromal Cells in Perfused 3D Ceramic Scaffolds Enhances In Vivo Bone Formation
journal, September 2017
- Hoch, Allison I.; Duhr, Ralph; Di Maggio, Nunzia
- Biotechnology Journal, Vol. 12, Issue 12
Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling
journal, June 2014
- Lin, R. -Z.; Moreno-Luna, R.; Li, D.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 28
Transferable cell-secreted extracellular matrices enhance osteogenic differentiation
journal, February 2012
- Decaris, Martin L.; Mojadedi, Azad; Bhat, Archana
- Acta Biomaterialia, Vol. 8, Issue 2
Strategies for MSC expansion and MSC-based microtissue for bone regeneration
journal, March 2019
- Bunpetch, Varitsara; Zhang, Zhi-Yong; Zhang, Xiaoan
- Biomaterials, Vol. 196
Design of Experiments Approach to Engineer Cell-Secreted Matrices for Directing Osteogenic Differentiation
journal, December 2010
- Decaris, Martin L.; Leach, J. Kent
- Annals of Biomedical Engineering, Vol. 39, Issue 4
Characterization and cost-benefit analysis of automated bioreactor-expanded mesenchymal stem cells for clinical applications: VALIDATION OF AUTOMATED BIOREACTOR
journal, September 2018
- Russell, Athena L.; Lefavor, Rebecca C.; Zubair, Abba C.
- Transfusion, Vol. 58, Issue 10
Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells
journal, July 2013
- Hernigou, Philippe; Homma, Yasuhiro; Flouzat Lachaniette, Charles Henri
- International Orthopaedics, Vol. 37, Issue 11
Hydrogel biophysical properties instruct coculture‐mediated osteogenic potential
journal, September 2015
- Murphy, Kaitlin C.; Stilhano, Roberta S.; Mitra, Debika
- The FASEB Journal, Vol. 30, Issue 1
featureCounts: an efficient general purpose program for assigning sequence reads to genomic features
journal, November 2013
- Liao, Y.; Smyth, G. K.; Shi, W.
- Bioinformatics, Vol. 30, Issue 7
Extracellular Matrix-Coated Composite Scaffolds Promote Mesenchymal Stem Cell Persistence and Osteogenesis
journal, September 2016
- Harvestine, Jenna N.; Vollmer, Nina L.; Ho, Steve S.
- Biomacromolecules, Vol. 17, Issue 11
Hypoxic Preconditioning Results in Increased Motility and Improved Therapeutic Potential of Human Mesenchymal Stem Cells
journal, August 2008
- Rosová, Ivana; Dao, Mo; Capoccia, Ben
- Stem Cells, Vol. 26, Issue 8
Tetrandrine identified in a small molecule screen to activate mesenchymal stem cells for enhanced immunomodulation
journal, July 2016
- Yang, Zijiang; Concannon, John; Ng, Kelvin S.
- Scientific Reports, Vol. 6, Issue 1
In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation
journal, February 2006
- Datta, N.; Pham, Q. P.; Sharma, U.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 8
Hypoxic Preconditioning of Mesenchymal Stromal Cells Induces Metabolic Changes, Enhances Survival, and Promotes Cell Retention In Vivo: Hypoxia Affects Metabolism and Survival of MSC
journal, May 2015
- Beegle, Julie; Lakatos, Kinga; Kalomoiris, Stefanos
- STEM CELLS, Vol. 33, Issue 6
Co-Seeding Human Endothelial Cells with Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells on Calcium Phosphate Scaffold Enhances Osteogenesis and Vascularization in Rats
journal, June 2017
- Liu, Xian; Chen, Wenchuan; Zhang, Chi
- Tissue Engineering Part A, Vol. 23, Issue 11-12
Three-Dimensional Perfusion Culture of Human Adipose Tissue-Derived Endothelial and Osteoblastic Progenitors Generates Osteogenic Constructs with Intrinsic Vascularization Capacity
journal, July 2007
- Scherberich, Arnaud; Galli, Raffaele; Jaquiery, Claude
- Stem Cells, Vol. 25, Issue 7
The Extracellular Matrix: Not Just Pretty Fibrils
journal, November 2009
- Hynes, Richard O.
- Science, Vol. 326, Issue 5957
Osteogenic comparison of expanded and uncultured adipose stromal cells
journal, July 2010
- Cheung, Whitney K.; Working, David M.; Galuppo, Larry D.
- Cytotherapy, Vol. 12, Issue 4
Stem Cells Combined With Platelet-rich Plasma Effectively Treat Corticosteroid-induced Osteonecrosis of the Hip: A Prospective Study
journal, January 2018
- Houdek, Matthew T.; Wyles, Cody C.; Collins, Mark S.
- Clinical Orthopaedics and Related Research, Vol. 476, Issue 2
Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue
journal, September 2011
- Güven, Sinan; Mehrkens, Arne; Saxer, Franziska
- Biomaterials, Vol. 32, Issue 25
Osteogenesis and Trophic Factor Secretion are Influenced by the Composition of Hydroxyapatite/Poly(Lactide-Co-Glycolide) Composite Scaffolds
journal, January 2010
- He, Jiawei; Genetos, Damian C.; Leach, J. Kent
- Tissue Engineering Part A, Vol. 16, Issue 1
Bioreactor culture duration of engineered constructs influences bone formation by mesenchymal stem cells
journal, November 2017
- Mitra, Debika; Whitehead, Jacklyn; Yasui, Osamu W.
- Biomaterials, Vol. 146
Hypoxic Preconditioning of Mesenchymal Stem Cells with Subsequent Spheroid Formation Accelerates Repair of Segmental Bone Defects: Hypoxic Preconditioning of MSCs for Bone Repair
journal, July 2018
- Ho, Steve S.; Hung, Ben P.; Heyrani, Nasser
- STEM CELLS, Vol. 36, Issue 9
Enhancing the biological performance of synthetic polymeric materials by decoration with engineered, decellularized extracellular matrix
journal, July 2012
- Sadr, Nasser; Pippenger, Benjamin E.; Scherberich, Arnaud
- Biomaterials, Vol. 33, Issue 20
Priming of endothelial colony‐forming cells in a mesenchymal niche improves engraftment and vasculogenic potential by initiating mesenchymal transition orchestrated by NOTCH signaling
journal, October 2016
- Shafiee, Abbas; Patel, Jatin; Yi Wong, Ho
- The FASEB Journal, Vol. 31, Issue 2
Mapping RNA‐seq Reads with STAR
journal, September 2015
- Dobin, Alexander; Gingeras, Thomas R.
- Current Protocols in Bioinformatics, Vol. 51, Issue 1
Preconditioning influences mesenchymal stem cell properties in vitro and in vivo
journal, February 2018
- Hu, Chenxia; Li, Lanjuan
- Journal of Cellular and Molecular Medicine, Vol. 22, Issue 3
Mixed extracellular matrix ligands synergistically modulate integrin adhesion and signaling
journal, November 2008
- Reyes, Catherine D.; Petrie, Timothy A.; García, Andrés J.
- Journal of Cellular Physiology, Vol. 217, Issue 2
ToppGene Suite for gene list enrichment analysis and candidate gene prioritization
journal, May 2009
- Chen, J.; Bardes, E. E.; Aronow, B. J.
- Nucleic Acids Research, Vol. 37, Issue Web Server
Flow Perfusion Co-culture of Human Mesenchymal Stem Cells and Endothelial Cells on Biodegradable Polymer Scaffolds
journal, July 2013
- Dahlin, Rebecca L.; Gershovich, Julia G.; Kasper, F. Kurtis
- Annals of Biomedical Engineering, Vol. 42, Issue 7
Engineered Fibrin Gels for Parallel Stimulation of Mesenchymal Stem Cell Proangiogenic and Osteogenic Potential
journal, December 2014
- Murphy, Kaitlin C.; Hughbanks, Marissa L.; Binder, Bernard Y. K.
- Annals of Biomedical Engineering, Vol. 43, Issue 8
Analysis of Osteocalcin Expression in Transgenic Mice Reveals a Species Difference in Vitamin D Regulation of Mouse and Human Osteocalcin Genes
journal, October 1997
- Clemens, Thomas L.; Tang, Hui; Maeda, Shigeto
- Journal of Bone and Mineral Research, Vol. 12, Issue 10
voom: precision weights unlock linear model analysis tools for RNA-seq read counts
journal, January 2014
- Law, Charity W.; Chen, Yunshun; Shi, Wei
- Genome Biology, Vol. 15, Issue 2
VEGF Scaffolds Enhance Angiogenesis and Bone Regeneration in Irradiated Osseous Defects
journal, February 2006
- Kaigler, Darnell; Wang, Zhuo; Horger, Kim
- Journal of Bone and Mineral Research, Vol. 21, Issue 5
VEGF: an Essential Mediator of Both Angiogenesis and Endochondral Ossification
journal, October 2007
- Dai, J.; Rabie, A. B. M.
- Journal of Dental Research, Vol. 86, Issue 10
One-Step Cartilage Repair with Bone Marrow Aspirate Concentrated Cells and Collagen Matrix in Full-Thickness Knee Cartilage Lesions: Results at 2-Year Follow-up
journal, February 2011
- Gobbi, Alberto; Karnatzikos, Georgios; Scotti, Celeste
- CARTILAGE, Vol. 2, Issue 3
Cell-secreted matrices perpetuate the bone-forming phenotype of differentiated mesenchymal stem cells
journal, January 2016
- Hoch, Allison I.; Mittal, Vaishali; Mitra, Debika
- Biomaterials, Vol. 74
Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity
journal, September 2003
- Wendt, D.; Marsano, A.; Jakob, M.
- Biotechnology and Bioengineering, Vol. 84, Issue 2
Mixed extracellular matrix ligands synergistically modulate integrin adhesion and signaling
journal, November 2008
- Reyes, Catherine D.; Petrie, Timothy A.; García, Andrés J.
- Journal of Cellular Physiology, Vol. 217, Issue 2
Hypoxic Preconditioning of Mesenchymal Stem Cells with Subsequent Spheroid Formation Accelerates Repair of Segmental Bone Defects: Hypoxic Preconditioning of MSCs for Bone Repair
journal, July 2018
- Ho, Steve S.; Hung, Ben P.; Heyrani, Nasser
- STEM CELLS, Vol. 36, Issue 9
Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells
journal, July 2013
- Hernigou, Philippe; Homma, Yasuhiro; Flouzat Lachaniette, Charles Henri
- International Orthopaedics, Vol. 37, Issue 11
Design of Experiments Approach to Engineer Cell-Secreted Matrices for Directing Osteogenic Differentiation
journal, December 2010
- Decaris, Martin L.; Leach, J. Kent
- Annals of Biomedical Engineering, Vol. 39, Issue 4
Engineered Fibrin Gels for Parallel Stimulation of Mesenchymal Stem Cell Proangiogenic and Osteogenic Potential
journal, December 2014
- Murphy, Kaitlin C.; Hughbanks, Marissa L.; Binder, Bernard Y. K.
- Annals of Biomedical Engineering, Vol. 43, Issue 8
CORR Insights®: Stem Cells Combined With Platelet-rich Plasma Effectively Treat Corticosteroid-induced Osteonecrosis of the Hip: A Prospective Study
journal, January 2018
- Vail, Thomas Parker
- Clinical Orthopaedics & Related Research, Vol. 476, Issue 2
Transferable cell-secreted extracellular matrices enhance osteogenic differentiation
journal, February 2012
- Decaris, Martin L.; Mojadedi, Azad; Bhat, Archana
- Acta Biomaterialia, Vol. 8, Issue 2
The role of ECM proteins and protein fragments in guiding cell behavior in regenerative medicine
journal, June 2011
- Barker, Thomas H.
- Biomaterials, Vol. 32, Issue 18
Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue
journal, September 2011
- Güven, Sinan; Mehrkens, Arne; Saxer, Franziska
- Biomaterials, Vol. 32, Issue 25
Enhancing the biological performance of synthetic polymeric materials by decoration with engineered, decellularized extracellular matrix
journal, July 2012
- Sadr, Nasser; Pippenger, Benjamin E.; Scherberich, Arnaud
- Biomaterials, Vol. 33, Issue 20
Cell-secreted matrices perpetuate the bone-forming phenotype of differentiated mesenchymal stem cells
journal, January 2016
- Hoch, Allison I.; Mittal, Vaishali; Mitra, Debika
- Biomaterials, Vol. 74
Strategies for MSC expansion and MSC-based microtissue for bone regeneration
journal, March 2019
- Bunpetch, Varitsara; Zhang, Zhi-Yong; Zhang, Xiaoan
- Biomaterials, Vol. 196
Quantifying Mesenchymal Stem Cells in the Mononuclear Cell Fraction of Bone Marrow Samples Obtained for Cell Therapy
journal, January 2013
- Alvarez-Viejo, M.; Menendez-Menendez, Y.; Blanco-Gelaz, M. A.
- Transplantation Proceedings, Vol. 45, Issue 1
Protein and Mineral Composition of Osteogenic Extracellular Matrix Constructs Generated with a Flow Perfusion Bioreactor
journal, December 2011
- Thibault, Richard A.; Mikos, Antonios G.; Kasper, F. Kurtis
- Biomacromolecules, Vol. 12, Issue 12
Tetrandrine identified in a small molecule screen to activate mesenchymal stem cells for enhanced immunomodulation
journal, July 2016
- Yang, Zijiang; Concannon, John; Ng, Kelvin S.
- Scientific Reports, Vol. 6, Issue 1
Cell-secreted extracellular matrix, independent of cell source, promotes the osteogenic differentiation of human stromal vascular fraction
journal, January 2018
- Harvestine, Jenna N.; Orbay, Hakan; Chen, Jonathan Y.
- Journal of Materials Chemistry B, Vol. 6, Issue 24
Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts
journal, January 2019
- Harvestine, Jenna N.; Saiz, Jr., Augustine M.; Leach, J. Kent
- Biomaterials Science, Vol. 7, Issue 5
In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation
journal, February 2006
- Datta, N.; Pham, Q. P.; Sharma, U.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 8
Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling
journal, June 2014
- Lin, R. -Z.; Moreno-Luna, R.; Li, D.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 28
In vitro biomimetic engineering of a human hematopoietic niche with functional properties
journal, June 2018
- Bourgine, Paul E.; Klein, Thibaut; Paczulla, Anna M.
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 25
Fluid flow increases mineralized matrix deposition in 3D perfusion culture of marrow stromal osteoblasts in a dose-dependent manner
journal, September 2002
- Bancroft, G. N.; Sikavitsas, V. I.; van den Dolder, J.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 20, p. 12600-12605
Osteogenesis and Trophic Factor Secretion are Influenced by the Composition of Hydroxyapatite/Poly(Lactide-Co-Glycolide) Composite Scaffolds
journal, January 2010
- He, Jiawei; Genetos, Damian C.; Leach, J. Kent
- Tissue Engineering Part A, Vol. 16, Issue 1
Establishment of an Early Vascular Network Promotes the Formation of Ectopic Bone
journal, February 2016
- Eman, Rhandy M.; Meijer, Henriette A. W.; Öner, F. Cumhur
- Tissue Engineering Part A, Vol. 22, Issue 3-4
ToppGene Suite for gene list enrichment analysis and candidate gene prioritization
journal, May 2009
- Chen, J.; Bardes, E. E.; Aronow, B. J.
- Nucleic Acids Research, Vol. 37, Issue Web Server
Hydrogel biophysical properties instruct coculture‐mediated osteogenic potential
journal, September 2015
- Murphy, Kaitlin C.; Stilhano, Roberta S.; Mitra, Debika
- The FASEB Journal, Vol. 30, Issue 1
Priming of endothelial colony‐forming cells in a mesenchymal niche improves engraftment and vasculogenic potential by initiating mesenchymal transition orchestrated by NOTCH signaling
journal, October 2016
- Shafiee, Abbas; Patel, Jatin; Yi Wong, Ho
- The FASEB Journal, Vol. 31, Issue 2
Preconditioning influences mesenchymal stem cell properties in vitro and in vivo
journal, February 2018
- Hu, Chenxia; Li, Lanjuan
- Journal of Cellular and Molecular Medicine, Vol. 22, Issue 3
The Extracellular Matrix: Not Just Pretty Fibrils
journal, November 2009
- Hynes, Richard O.
- Science, Vol. 326, Issue 5957
voom: precision weights unlock linear model analysis tools for RNA-seq read counts
journal, January 2014
- Law, Charity W.; Chen, Yunshun; Shi, Wei
- Genome Biology, Vol. 15, Issue 2
Cartilage to bone transformation during fracture healing is coordinated by the invading vasculature and induction of the core pluripotency genes
journal, January 2017
- Hu, Diane P.; Ferro, Federico; Yang, Frank
- Development, Vol. 144, Issue 2
VEGF Scaffolds Enhance Angiogenesis and Bone Regeneration in Irradiated Osseous Defects
journal, February 2006
- Kaigler, Darnell; Wang, Zhuo; Horger, Kim
- Journal of Bone and Mineral Research, Vol. 21, Issue 5
Analysis of Osteocalcin Expression in Transgenic Mice Reveals a Species Difference in Vitamin D Regulation of Mouse and Human Osteocalcin Genes
journal, October 1997
- Clemens, Thomas L.; Tang, Hui; Maeda, Shigeto
- Journal of Bone and Mineral Research, Vol. 12, Issue 10
Differentiation-Dependent Secretion of Proangiogenic Factors by Mesenchymal Stem Cells
journal, April 2012
- Hoch, Allison I.; Binder, Bernard Y.; Genetos, Damian C.
- PLoS ONE, Vol. 7, Issue 4
Three-Dimensional Perfusion Culture of Human Adipose Tissue-Derived Endothelial and Osteoblastic Progenitors Generates Osteogenic Constructs with Intrinsic Vascularization Capacity
journal, July 2007
- Scherberich, Arnaud; Galli, Raffaele; Jaquiery, Claude
- Stem Cells, Vol. 25, Issue 7
Hypoxic Preconditioning Results in Increased Motility and Improved Therapeutic Potential of Human Mesenchymal Stem Cells
journal, August 2008
- Rosová, Ivana; Dao, Mo; Capoccia, Ben
- Stem Cells, Vol. 26, Issue 8
Intraoperative engineering of osteogenic grafts combining freshly harvested, human adipose-derived cells and physiological doses of bone morphogenetic protein-2
journal, September 2012
- Mehrkens, A.; Saxer, F.; Güven, S.
- European Cells and Materials, Vol. 24
Dlx5, a Positive Regulator of Osteoblastogenesis, is Essential for Osteoblast-Osteoclast Coupling
journal, September 2008
- Samee, Nadeem; Geoffroy, Valerie; Marty, Caroline
- The American Journal of Pathology, Vol. 173, Issue 3
Osteogenic comparison of expanded and uncultured adipose stromal cells
journal, July 2010
- Cheung, Whitney K.; Working, David M.; Galuppo, Larry D.
- Cytotherapy, Vol. 12, Issue 4
featureCounts: An efficient general-purpose program for assigning sequence reads to genomic features
text, January 2013
- Liao, Yang; Smyth, Gordon K.; Shi, Wei
- arXiv
Figures / Tables found in this record: