Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii
Abstract
Many details of structure, function and substrate specificity of eukaryotic proteasomal systems have been elucidated. This information far-exceeds that available for the archaeal and bacterial counterparts. While structural and functional studies have provided some insight into the workings of prokaryotic proteasomes, the question of substrate targeting and global cellular influence remain largely unaddressed. In this communication, we report an over 720-fold increase in the half-life of the DNA-sliding clamp protein proliferating cell nuclear antigen after knockout of the panA gene, encoding a proteasome-activating nucleotidase A, on the chromosome of the halophilic archaeon Haloferax volcanii. This discovery marks the first identification of a protein stabilized by an archaeal proteasome mutation and provides a starting point for investigations into substrate recognition mechanisms. The findings also begin to address the functional role of proteasomal systems within the scope of the archaeal cell.
- Authors:
-
- Univ. of Florida, Gainesville, FL (United States). Dept. of Microbiology and Cell Science
- Publication Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1625903
- Grant/Contract Number:
- FG02-05ER15650
- Resource Type:
- Accepted Manuscript
- Journal Name:
- FEMS Microbiology Letters
- Additional Journal Information:
- Journal Volume: 294; Journal Issue: 1; Journal ID: ISSN 0378-1097
- Publisher:
- Federation of European Microbiological Societies
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology; archaea; proteasome; halophile; DNA polymerase; sliding clamp; proteolysis
Citation Formats
Kirkland, P. Aaron, and Maupin-Furlow, Julie A. Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii. United States: N. p., 2009.
Web. doi:10.1111/j.1574-6968.2009.01547.x.
Kirkland, P. Aaron, & Maupin-Furlow, Julie A. Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii. United States. https://doi.org/10.1111/j.1574-6968.2009.01547.x
Kirkland, P. Aaron, and Maupin-Furlow, Julie A. Fri .
"Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii". United States. https://doi.org/10.1111/j.1574-6968.2009.01547.x. https://www.osti.gov/servlets/purl/1625903.
@article{osti_1625903,
title = {Stabilization of an archaeal DNA-sliding clamp protein, PCNA, by proteasome-activating nucleotidase gene knockout in Haloferax volcanii},
author = {Kirkland, P. Aaron and Maupin-Furlow, Julie A.},
abstractNote = {Many details of structure, function and substrate specificity of eukaryotic proteasomal systems have been elucidated. This information far-exceeds that available for the archaeal and bacterial counterparts. While structural and functional studies have provided some insight into the workings of prokaryotic proteasomes, the question of substrate targeting and global cellular influence remain largely unaddressed. In this communication, we report an over 720-fold increase in the half-life of the DNA-sliding clamp protein proliferating cell nuclear antigen after knockout of the panA gene, encoding a proteasome-activating nucleotidase A, on the chromosome of the halophilic archaeon Haloferax volcanii. This discovery marks the first identification of a protein stabilized by an archaeal proteasome mutation and provides a starting point for investigations into substrate recognition mechanisms. The findings also begin to address the functional role of proteasomal systems within the scope of the archaeal cell.},
doi = {10.1111/j.1574-6968.2009.01547.x},
journal = {FEMS Microbiology Letters},
number = 1,
volume = 294,
place = {United States},
year = {Fri May 01 00:00:00 EDT 2009},
month = {Fri May 01 00:00:00 EDT 2009}
}
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Works referencing / citing this record:
Proteasomes and protein conjugation across domains of life
journal, December 2011
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Lymph node nomenclature in gynecologic oncology
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