Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source
Abstract
The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world's mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments of the limitations and advantages of a combined SAXS and MX beamline have suggested new strategies for integration and optimal data collection methods and have led to additional hardware and software enhancements. Features described include a dual mode monochromator [containing both Si(111) crystals and Mo/B4C multilayer elements], rapid beamline optics conversion between SAXS and MX modes, active beam stabilization, sample-loading robotics, and mail-in and remote data collection. These features allow users to gain valuable insights from both dynamic solution scattering and high-resolution atomic diffraction experiments performed at a single synchrotron beamline. Key practical issues considered for data collection and analysis include radiation damage, structural ensembles, alternative conformers and flexibility. SIBYLS develops and applies efficient combined MX and SAXS methods that deliver high-impact results by providing robust cost-effective routes to connect structures to biology and by performing experiments that aid beamline designs for next generationmore »
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Bioscience Division
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Bioscience Division; Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Engineering Division
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division
- OSTI Identifier:
- 1625650
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Applied Crystallography
- Additional Journal Information:
- Journal Volume: 46; Journal Issue: 1; Journal ID: ISSN 0021-8898
- Publisher:
- International Union of Crystallography
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; chemistry; crystallography; small-angle X-ray scattering (SAXS); macromolecular crystallography (MX); synchrotron beamlines; SIBYLS
Citation Formats
Classen, Scott, Hura, Greg L., Holton, James M., Rambo, Robert P., Rodic, Ivan, McGuire, Patrick J., Dyer, Kevin, Hammel, Michal, Meigs, George, Frankel, Kenneth A., and Tainer, John A. Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source. United States: N. p., 2013.
Web. doi:10.1107/s0021889812048698.
Classen, Scott, Hura, Greg L., Holton, James M., Rambo, Robert P., Rodic, Ivan, McGuire, Patrick J., Dyer, Kevin, Hammel, Michal, Meigs, George, Frankel, Kenneth A., & Tainer, John A. Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source. United States. https://doi.org/10.1107/s0021889812048698
Classen, Scott, Hura, Greg L., Holton, James M., Rambo, Robert P., Rodic, Ivan, McGuire, Patrick J., Dyer, Kevin, Hammel, Michal, Meigs, George, Frankel, Kenneth A., and Tainer, John A. Thu .
"Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source". United States. https://doi.org/10.1107/s0021889812048698. https://www.osti.gov/servlets/purl/1625650.
@article{osti_1625650,
title = {Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source},
author = {Classen, Scott and Hura, Greg L. and Holton, James M. and Rambo, Robert P. and Rodic, Ivan and McGuire, Patrick J. and Dyer, Kevin and Hammel, Michal and Meigs, George and Frankel, Kenneth A. and Tainer, John A.},
abstractNote = {The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world's mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments of the limitations and advantages of a combined SAXS and MX beamline have suggested new strategies for integration and optimal data collection methods and have led to additional hardware and software enhancements. Features described include a dual mode monochromator [containing both Si(111) crystals and Mo/B4C multilayer elements], rapid beamline optics conversion between SAXS and MX modes, active beam stabilization, sample-loading robotics, and mail-in and remote data collection. These features allow users to gain valuable insights from both dynamic solution scattering and high-resolution atomic diffraction experiments performed at a single synchrotron beamline. Key practical issues considered for data collection and analysis include radiation damage, structural ensembles, alternative conformers and flexibility. SIBYLS develops and applies efficient combined MX and SAXS methods that deliver high-impact results by providing robust cost-effective routes to connect structures to biology and by performing experiments that aid beamline designs for next generation light sources.},
doi = {10.1107/s0021889812048698},
journal = {Journal of Applied Crystallography},
number = 1,
volume = 46,
place = {United States},
year = {Thu Jan 17 00:00:00 EST 2013},
month = {Thu Jan 17 00:00:00 EST 2013}
}
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