Regulation and Levels of the Thylakoid K + /H + Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light
Abstract
Crop canopies create environments of highly fluctuating light intensities. In such environments, photoprotective mechanisms and their relaxation kinetics have been hypothesized to limit photosynthetic efficiency and therefore crop yield potential. Here, we show that overexpression of the Arabidopsis thylakoid K+/H+ antiporter KEA3 accelerates the relaxation of photoprotective energy-dependent quenching after transitions from high to low light in Arabidopsis and tobacco. This, in turn, enhances PSII quantum efficiency in both organisms, supporting that in wild-type plants, residual light energy quenching following a high to low light transition represents a limitation to photosynthetic efficiency in fluctuating light. This finding underscores the potential of accelerating quenching relaxation as a building block for improving photosynthetic efficiency in the field. Additionally, by overexpressing natural KEA3 variants with modification to the C-terminus, we show that KEA3 activity is regulated by a mechanism involving its lumen-localized C-terminus, which lowers KEA3 activity in high light. This regulatory mechanism finetunes the balance between photoprotective energy dissipation in high light and maximum quantum yield in low light, likely to be critical for efficient photosynthesis in fluctuating light conditions.
- Authors:
-
- Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology. Howard Hughes Medical Inst.; Carnegie Inst. for Science, Stanford, CA (United States). Dept. of Plant Biology; Max Planck Inst. of Molecular Plant Physiology, Potsdam (Germany)
- Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology. Howard Hughes Medical Inst.
- Max Planck Inst. of Molecular Plant Physiology, Potsdam (Germany)
- Carnegie Inst. for Science, Stanford, CA (United States). Dept. of Plant Biology
- Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology. Howard Hughes Medical Inst.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
- OSTI Identifier:
- 1625582
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plant and Cell Physiology
- Additional Journal Information:
- Journal Volume: 57; Journal Issue: 7; Journal ID: ISSN 0032-0781
- Publisher:
- Japanese Society of Plant Physiologists
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Plant Sciences; Cell Biology; Arabidopsis; KEA3; Non-photochemical quenching; PSII quantum efficiency; Thylakoid membrane
Citation Formats
Armbruster, Ute, Leonelli, Lauriebeth, Correa Galvis, Viviana, Strand, Deserah, Quinn, Erica H., Jonikas, Martin C., and Niyogi, Krishna K. Regulation and Levels of the Thylakoid K + /H + Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light. United States: N. p., 2016.
Web. doi:10.1093/pcp/pcw085.
Armbruster, Ute, Leonelli, Lauriebeth, Correa Galvis, Viviana, Strand, Deserah, Quinn, Erica H., Jonikas, Martin C., & Niyogi, Krishna K. Regulation and Levels of the Thylakoid K + /H + Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light. United States. https://doi.org/10.1093/pcp/pcw085
Armbruster, Ute, Leonelli, Lauriebeth, Correa Galvis, Viviana, Strand, Deserah, Quinn, Erica H., Jonikas, Martin C., and Niyogi, Krishna K. Fri .
"Regulation and Levels of the Thylakoid K + /H + Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light". United States. https://doi.org/10.1093/pcp/pcw085. https://www.osti.gov/servlets/purl/1625582.
@article{osti_1625582,
title = {Regulation and Levels of the Thylakoid K + /H + Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light},
author = {Armbruster, Ute and Leonelli, Lauriebeth and Correa Galvis, Viviana and Strand, Deserah and Quinn, Erica H. and Jonikas, Martin C. and Niyogi, Krishna K.},
abstractNote = {Crop canopies create environments of highly fluctuating light intensities. In such environments, photoprotective mechanisms and their relaxation kinetics have been hypothesized to limit photosynthetic efficiency and therefore crop yield potential. Here, we show that overexpression of the Arabidopsis thylakoid K+/H+ antiporter KEA3 accelerates the relaxation of photoprotective energy-dependent quenching after transitions from high to low light in Arabidopsis and tobacco. This, in turn, enhances PSII quantum efficiency in both organisms, supporting that in wild-type plants, residual light energy quenching following a high to low light transition represents a limitation to photosynthetic efficiency in fluctuating light. This finding underscores the potential of accelerating quenching relaxation as a building block for improving photosynthetic efficiency in the field. Additionally, by overexpressing natural KEA3 variants with modification to the C-terminus, we show that KEA3 activity is regulated by a mechanism involving its lumen-localized C-terminus, which lowers KEA3 activity in high light. This regulatory mechanism finetunes the balance between photoprotective energy dissipation in high light and maximum quantum yield in low light, likely to be critical for efficient photosynthesis in fluctuating light conditions.},
doi = {10.1093/pcp/pcw085},
journal = {Plant and Cell Physiology},
number = 7,
volume = 57,
place = {United States},
year = {Fri May 06 00:00:00 EDT 2016},
month = {Fri May 06 00:00:00 EDT 2016}
}
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