Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator
Abstract
Cardamine violifolia (family Brassicaceae) is the first discovered selenium hyperaccumulator from the genus Cardamine with unique properties in terms of selenium accumulation, i.e., high abundance of selenolanthionine. In our study, a fully comprehensive experiment was conducted with the comparison of a non-hyperaccumulator Cardamine species, Cardamine pratensis, covering growth characteristics, chlorophyll fluorescence, spatial selenium/sulfur distribution patterns through elemental analyses (synchrotron-based X-Ray Fluorescence and ICP-OES) and speciation data through selenium K-edge micro X-ray absorption near-edge structure analysis (μXANES) and strong cation exchange (SCX)-ICP-MS. The results revealed remarkable differences in contrast to other selenium hyperaccumulators as neither Cardamine species showed evidence of growth stimulation by selenium. Also, selenite uptake was not inhibited by phosphate for either of the Cardamine species. Sulfate inhibited selenate uptake, but the two Cardamine species did not show any difference in this respect. However, μXRF derived speciation maps and selenium/sulfur uptake characteristics provided results that are similar to other formerly reported hyperaccumulator and non-hyperaccumulator Brassicaceae species. μXANES showed organic selenium, "C-Se-C", in seedlings of both species and also in mature C. violifolia plants. In contrast, selenate-supplied mature C. pratensis contained approximately half "C-Se-C" and half selenate. SCX-ICP-MS data showed evidence of the lack of selenocystine in any of themore »
- Authors:
-
- Szent István University, Budapest (Hungary). Dept. of Applied Chemistry; Colorado State Univ., Fort Collins, CO (United States). Dept. of Biology
- Colorado State Univ., Fort Collins, CO (United States). Dept. of Biology
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source
- Laramie County Community College, Cheyenne, WY (United States). Biology Department
- Enshi Autonomous Prefecture Academy of Agriculture Sciences (China)
- Szent István University, Budapest (Hungary). Dept. of Applied Chemistry
- University of Debrecen (Hungary). Institute of Food Science, Faculty of Agricultural and Food Sciences and Environmental Management
- Hungarian Academy of Sciences (Hungary). Department of Plant Physiology, Agricultural Institute, Centre for Agricultural Research
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); Campus Mundi Program (Hungary); National Natural Science Foundation of China (NSFC); National Institutes of Health (NIH); National Science Foundation (NSF)
- OSTI Identifier:
- 1623712
- Alternate Identifier(s):
- OSTI ID: 1760215
- Grant/Contract Number:
- AC02-05CH11231; EFOP-3.4.2-VEKOP-15-2015-000; 3156110472; 2P20GM103432; 1456361
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Science of the Total Environment
- Additional Journal Information:
- Journal Volume: 703; Journal Issue: C; Journal ID: ISSN 0048-9697
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Environmental Sciences & Ecology
Citation Formats
Both, Eszter Borbála, Stonehouse, Gavin C., Lima, Leonardo Warzea, Fakra, Sirine C., Aguirre, Bernadette, Wangeline, Ami L., Xiang, Jiqian, Yin, Hongqing, Jókai, Zsuzsa, Soós, Áron, Dernovics, Mihály, and Pilon-Smits, Elizabeth A. H. Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator. United States: N. p., 2020.
Web. doi:10.1016/j.scitotenv.2019.135041.
Both, Eszter Borbála, Stonehouse, Gavin C., Lima, Leonardo Warzea, Fakra, Sirine C., Aguirre, Bernadette, Wangeline, Ami L., Xiang, Jiqian, Yin, Hongqing, Jókai, Zsuzsa, Soós, Áron, Dernovics, Mihály, & Pilon-Smits, Elizabeth A. H. Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator. United States. https://doi.org/10.1016/j.scitotenv.2019.135041
Both, Eszter Borbála, Stonehouse, Gavin C., Lima, Leonardo Warzea, Fakra, Sirine C., Aguirre, Bernadette, Wangeline, Ami L., Xiang, Jiqian, Yin, Hongqing, Jókai, Zsuzsa, Soós, Áron, Dernovics, Mihály, and Pilon-Smits, Elizabeth A. H. Sat .
"Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator". United States. https://doi.org/10.1016/j.scitotenv.2019.135041. https://www.osti.gov/servlets/purl/1623712.
@article{osti_1623712,
title = {Selenium tolerance, accumulation, localization and speciation in a Cardamine hyperaccumulator and a non-hyperaccumulator},
author = {Both, Eszter Borbála and Stonehouse, Gavin C. and Lima, Leonardo Warzea and Fakra, Sirine C. and Aguirre, Bernadette and Wangeline, Ami L. and Xiang, Jiqian and Yin, Hongqing and Jókai, Zsuzsa and Soós, Áron and Dernovics, Mihály and Pilon-Smits, Elizabeth A. H.},
abstractNote = {Cardamine violifolia (family Brassicaceae) is the first discovered selenium hyperaccumulator from the genus Cardamine with unique properties in terms of selenium accumulation, i.e., high abundance of selenolanthionine. In our study, a fully comprehensive experiment was conducted with the comparison of a non-hyperaccumulator Cardamine species, Cardamine pratensis, covering growth characteristics, chlorophyll fluorescence, spatial selenium/sulfur distribution patterns through elemental analyses (synchrotron-based X-Ray Fluorescence and ICP-OES) and speciation data through selenium K-edge micro X-ray absorption near-edge structure analysis (μXANES) and strong cation exchange (SCX)-ICP-MS. The results revealed remarkable differences in contrast to other selenium hyperaccumulators as neither Cardamine species showed evidence of growth stimulation by selenium. Also, selenite uptake was not inhibited by phosphate for either of the Cardamine species. Sulfate inhibited selenate uptake, but the two Cardamine species did not show any difference in this respect. However, μXRF derived speciation maps and selenium/sulfur uptake characteristics provided results that are similar to other formerly reported hyperaccumulator and non-hyperaccumulator Brassicaceae species. μXANES showed organic selenium, "C-Se-C", in seedlings of both species and also in mature C. violifolia plants. In contrast, selenate-supplied mature C. pratensis contained approximately half "C-Se-C" and half selenate. SCX-ICP-MS data showed evidence of the lack of selenocystine in any of the Cardamine plant extracts. Thus, C. violifolia shows clear selenium-related physiological and biochemical differences compared to C. pratensis and other selenium hyperaccumulators.},
doi = {10.1016/j.scitotenv.2019.135041},
journal = {Science of the Total Environment},
number = C,
volume = 703,
place = {United States},
year = {Sat Feb 01 00:00:00 EST 2020},
month = {Sat Feb 01 00:00:00 EST 2020}
}
Figures / Tables:
Works referenced in this record:
Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae)
journal, November 2014
- Alford, Élan R.; Lindblom, Stormy D.; Pittarello, Marco
- American Journal of Botany, Vol. 101, Issue 11
Selenium Accumulation, Distribution, and Speciation in Spineless Prickly Pear Cactus: A Drought- and Salt-Tolerant, Selenium-Enriched Nutraceutical Fruit Crop for Biofortified Foods
journal, November 2010
- Bañuelos, Gary S.; Fakra, Sirine C.; Walse, Spencer S.
- Plant Physiology, Vol. 155, Issue 1
Selenolanthionine is the major water-soluble selenium compound in the selenium tolerant plant Cardamine violifolia
journal, November 2018
- Both, Eszter Borbála; Shao, Shuxun; Xiang, Jiqian
- Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1862, Issue 11
Characterization of selenium and sulfur accumulation across the genus Stanleya (Brassicaceae): A field survey and common-garden experiment
journal, May 2014
- Cappa, Jennifer J.; Cappa, Patrick J.; El Mehdawi, Ali F.
- American Journal of Botany, Vol. 101, Issue 5
Selenium translocation in the soil-rice system in the Enshi seleniferous area, Central China
journal, June 2019
- Chang, Chuanyu; Yin, Runsheng; Wang, Xun
- Science of The Total Environment, Vol. 669
Large-scale speciation of selenium in rice proteins using ICP-MS assisted electrospray MS/MS proteomics
journal, January 2014
- Cheajesadagul, Pracha; Bianga, Juliusz; Arnaudguilhem, Carine
- Metallomics, Vol. 6, Issue 3
Spatial and Temporal Localization of Flavonoid Metabolites in Strawberry Fruit ( Fragaria × ananassa )
journal, April 2017
- Crecelius, Anna C.; Hölscher, Dirk; Hoffmann, Thomas
- Journal of Agricultural and Food Chemistry, Vol. 65, Issue 17
Effect of Sulfate on Selenium Uptake and Chemical Speciation in Convolvulus arvensis L.
journal, January 2005
- Cruz-Jímenez, Gustavo; Peralta-Videa, Jose R.; de la Rosa, Guadalupe
- Environmental Chemistry, Vol. 2, Issue 2
Translocation and transformation of selenium in hyperaccumulator plant Cardamine enshiensis from Enshi, Hubei, China
journal, February 2018
- Cui, Liwei; Zhao, Jiating; Chen, Jiuyu
- Plant and Soil, Vol. 425, Issue 1-2
Rate-Limiting Steps in Selenium Assimilation and Volatilization by Indian Mustard
journal, August 1998
- de Souza, Mark P.; Pilon-Smits, Elizabeth A. H.; Lytle, C. Mel
- Plant Physiology, Vol. 117, Issue 4
Selenium distribution and speciation in plant parts of wheat (Triticum aestivum) and Indian mustard (Brassica juncea) from a seleniferous area of Punjab, India
journal, February 2015
- Eiche, E.; Bardelli, F.; Nothstein, A. K.
- Science of The Total Environment, Vol. 505
Interactions of selenium hyperaccumulators and nonaccumulators during cocultivation on seleniferous or nonseleniferous soil - the importance of having good neighbors
journal, January 2012
- El Mehdawi, Ali F.; Cappa, Jennifer J.; Fakra, Sirine C.
- New Phytologist, Vol. 194, Issue 1
Influence of sulfate supply on selenium uptake dynamics and expression of sulfate/selenate transporters in selenium hyperaccumulator and nonhyperaccumulator Brassicaceae
journal, October 2017
- El Mehdawi, Ali F.; Jiang, Ying; Guignardi, Zack S.
- New Phytologist, Vol. 217, Issue 1
Analysis of selenium accumulation, speciation and tolerance of potential selenium hyperaccumulator Symphyotrichum ericoides
journal, February 2014
- El Mehdawi, Ali F.; Reynolds, Ray Jason B.; Prins, Christine N.
- Physiologia Plantarum, Vol. 152, Issue 1
Correlative Cryogenic Spectromicroscopy to Investigate Selenium Bioreduction Products
journal, December 2017
- Fakra, Sirine C.; Luef, Birgit; Castelle, Cindy J.
- Environmental Science & Technology, Vol. 52, Issue 2
Quantitative Elemental Analyses by Plasma Emission Spectroscopy
journal, October 1978
- Fassel, V. A.
- Science, Vol. 202, Issue 4364
Soil, grain and water chemistry in relation to human selenium-responsive diseases in Enshi District, China
journal, January 2000
- Fordyce, F. M.; Guangdi, Zhang; Green, K.
- Applied Geochemistry, Vol. 15, Issue 1
Speciation analysis with HPLC-mass spectrometry: time to take stock
journal, January 2005
- Francesconi, Kevin A.; Sperling, Michael
- The Analyst, Vol. 130, Issue 7
Selenium Hyperaccumulator Plants Stanleya pinnata and Astragalus bisulcatus Are Colonized by Se-Resistant, Se-Excluding Wasp and Beetle Seed Herbivores
journal, December 2012
- Freeman, John L.; Marcus, Matthew A.; Fakra, Sirine C.
- PLoS ONE, Vol. 7, Issue 12
Molecular Mechanisms of Selenium Tolerance and Hyperaccumulation in Stanleya pinnata
journal, May 2010
- Freeman, John L.; Tamaoki, Masanori; Stushnoff, Cecil
- Plant Physiology, Vol. 153, Issue 4
Spatial Imaging, Speciation, and Quantification of Selenium in the Hyperaccumulator Plants Astragalus bisulcatus and Stanleya pinnata
journal, August 2006
- Freeman, John L.; Zhang, Li Hong; Marcus, Matthew A.
- Plant Physiology, Vol. 142, Issue 1
Sulfur–selenium–molybdenum interactions distinguish selenium hyperaccumulator Stanleya pinnata from non-hyperaccumulator Brassica juncea (Brassicaceae)
journal, November 2013
- Harris, Jonathan; Schneberg, Kathryn A.; Pilon-Smits, Elizabeth A. H.
- Planta, Vol. 239, Issue 2
Pairwise multiple comparison procedures: A review.
journal, January 1984
- Jaccard, James; Becker, Michael A.; Wood, Gregory
- Psychological Bulletin, Vol. 96, Issue 3
Inoculation of selenium hyperaccumulator Stanleya pinnata and related non-accumulator Stanleya elata with hyperaccumulator rhizosphere fungi-investigation of effects on Se accumulation and speciation
journal, September 2013
- Lindblom, Stormy Dawn; Fakra, Sirine C.; Landon, Jessica
- Physiologia Plantarum, Vol. 150, Issue 1
Spectrophotometric determination of antioxidant activity
journal, June 1996
- Miller, N. J.; Rice-Evans, C. A.
- Redox Report, Vol. 2, Issue 3
Initial Studies of Selenium Speciation in Brassica juncea by LC with ICPMS and ES-MS Detection: an Approach for Phytoremediation Studies
journal, January 2002
- Montes-Bayón, María; Yanes, Enrique G.; Ponce de León, Claudia
- Analytical Chemistry, Vol. 74, Issue 1
A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962
- Murashige, Toshio; Skoog, Folke
- Physiologia Plantarum, Vol. 15, Issue 3, p. 473-497
The relationship of selenium tolerance and speciation in Lecythidaceae species
journal, January 2013
- Németh, Anikó; García Reyes, Juan Francisco; Kosáry, Judit
- Metallomics, Vol. 5, Issue 12
Identification of selenohomolanthionine in selenium-enriched Japanese pungent radish
journal, January 2007
- Ogra, Yasumitsu; Kitaguchi, Takashi; Ishiwata, Kazuya
- Journal of Analytical Atomic Spectrometry, Vol. 22, Issue 11
Selenium fractions in organic matter from Se-rich soils and weathered stone coal in selenosis areas of China
journal, February 2012
- Qin, Hai-bo; Zhu, Jian-ming; Su, Hui
- Chemosphere, Vol. 86, Issue 6
ImageJ2: ImageJ for the next generation of scientific image data
journal, November 2017
- Rueden, Curtis T.; Schindelin, Johannes; Hiner, Mark C.
- BMC Bioinformatics, Vol. 18, Issue 1
High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress
journal, April 2018
- Sarabia, Lenin D.; Boughton, Berin A.; Rupasinghe, Thusitha
- Metabolomics, Vol. 14, Issue 5
The fascinating facets of plant selenium accumulation – biochemistry, physiology, evolution and ecology
journal, November 2016
- Schiavon, Michela; Pilon‐Smits, Elizabeth A. H.
- New Phytologist, Vol. 213, Issue 4
Accumulation and speciation of selenium in Cardamine sp. in Yutangba Se Mining Field, Enshi, China
journal, October 2014
- Shao, Shuxun; Deng, Guodong; Mi, Xiubo
- Chinese Journal of Geochemistry, Vol. 33, Issue 4
Physiological, biochemical, and ultrastructural characterization of selenium toxicity in cowpea plants
journal, June 2018
- Silva, Vinícius Martins; Boleta, Eduardo Henrique Marcandalli; Lanza, Maria Gabriela Dantas Bereta
- Environmental and Experimental Botany, Vol. 150
Characterization of selenocysteine methyltransferases from Astragalus species with contrasting selenium accumulation capacity
journal, July 2009
- Sors, Thomas G.; Martin, Catherine P.; Salt, David E.
- The Plant Journal, Vol. 59, Issue 1
Characterization and benefits of selenium uptake by an Astragalus hyperaccumulator and a non-accumulator
journal, March 2016
- Statwick, Joseph; Majestic, Brian J.; Sher, Anna A.
- Plant and Soil, Vol. 404, Issue 1-2
Transcriptome-wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome
journal, March 2018
- Wang, Jiameng; Cappa, Jennifer J.; Harris, Jonathan P.
- Plant Biotechnology Journal, Vol. 16, Issue 9
Extraordinarily High Leaf Selenium to Sulfur Ratios Define ‘Se-accumulator’ Plants
journal, May 2007
- White, Philip J.; Bowen, Helen C.; Marshall, Bruce
- Annals of Botany, Vol. 100, Issue 1
Concentrations and characteristics of selenium in soil samples from Dashan Region, a selenium-enriched area in China
journal, October 2015
- Xing, Kun; Zhou, Shoubiao; Wu, Xiaoguo
- Soil Science and Plant Nutrition, Vol. 61, Issue 6
Nitric acid digestion and multi‐element analysis of plant material by inductively coupled plasma spectrometry
journal, January 1987
- Zarcinas, B. A.; Cartwright, B.; Spouncer, L. R.
- Communications in Soil Science and Plant Analysis, Vol. 18, Issue 1
Distribution and transport of selenium in Yutangba, China: Impact of human activities
journal, March 2008
- Zhu, Jiangming; Wang, Ning; Li, Shehong
- Science of The Total Environment, Vol. 392, Issue 2-3
Occurrence of native selenium in Yutangba and its environmental implications
journal, March 2004
- Zhu, Jianming; Zuo, Wei; Liang, Xiaobing
- Applied Geochemistry, Vol. 19, Issue 3
Sulfur–selenium–molybdenum interactions distinguish selenium hyperaccumulator Stanleya pinnata from non-hyperaccumulator Brassica juncea (Brassicaceae)
journal, November 2013
- Harris, Jonathan; Schneberg, Kathryn A.; Pilon-Smits, Elizabeth A. H.
- Planta, Vol. 239, Issue 2
Biosynthesis of selenocystathionine from selenate in Stanleya pinnata
journal, January 1963
- Virupaksha, T. K.; Shrift, A.
- Biochimica et Biophysica Acta, Vol. 74
Selenium fractions in organic matter from Se-rich soils and weathered stone coal in selenosis areas of China
journal, February 2012
- Qin, Hai-bo; Zhu, Jian-ming; Su, Hui
- Chemosphere, Vol. 86, Issue 6
Selenium distribution and speciation in plant parts of wheat (Triticum aestivum) and Indian mustard (Brassica juncea) from a seleniferous area of Punjab, India
journal, February 2015
- Eiche, E.; Bardelli, F.; Nothstein, A. K.
- Science of The Total Environment, Vol. 505
Selenium translocation in the soil-rice system in the Enshi seleniferous area, Central China
journal, June 2019
- Chang, Chuanyu; Yin, Runsheng; Wang, Xun
- Science of The Total Environment, Vol. 669
Initial Studies of Selenium Speciation in Brassica juncea by LC with ICPMS and ES-MS Detection: an Approach for Phytoremediation Studies
journal, January 2002
- Montes-Bayón, María; Yanes, Enrique G.; Ponce de León, Claudia
- Analytical Chemistry, Vol. 74, Issue 1
Correlative Cryogenic Spectromicroscopy to Investigate Selenium Bioreduction Products
journal, December 2017
- Fakra, Sirine C.; Luef, Birgit; Castelle, Cindy J.
- Environmental Science & Technology, Vol. 52, Issue 2
Spatial and Temporal Localization of Flavonoid Metabolites in Strawberry Fruit ( Fragaria × ananassa )
journal, April 2017
- Crecelius, Anna C.; Hölscher, Dirk; Hoffmann, Thomas
- Journal of Agricultural and Food Chemistry, Vol. 65, Issue 17
Speciation analysis with HPLC-mass spectrometry: time to take stock
journal, January 2005
- Francesconi, Kevin A.; Sperling, Michael
- The Analyst, Vol. 130, Issue 7
The relationship of selenium tolerance and speciation in Lecythidaceae species
journal, January 2013
- Németh, Anikó; García Reyes, Juan Francisco; Kosáry, Judit
- Metallomics, Vol. 5, Issue 12
Large-scale speciation of selenium in rice proteins using ICP-MS assisted electrospray MS/MS proteomics
journal, January 2014
- Cheajesadagul, Pracha; Bianga, Juliusz; Arnaudguilhem, Carine
- Metallomics, Vol. 6, Issue 3
Spectrophotometric determination of antioxidant activity
journal, June 1996
- Miller, N. J.; Rice-Evans, C. A.
- Redox Report, Vol. 2, Issue 3
Extraordinarily High Leaf Selenium to Sulfur Ratios Define ‘Se-accumulator’ Plants
journal, May 2007
- White, Philip J.; Bowen, Helen C.; Marshall, Bruce
- Annals of Botany, Vol. 100, Issue 1
Molecular Mechanisms of Selenium Tolerance and Hyperaccumulation in Stanleya pinnata
journal, May 2010
- Freeman, John L.; Tamaoki, Masanori; Stushnoff, Cecil
- Plant Physiology, Vol. 153, Issue 4
Selenium Accumulation, Distribution, and Speciation in Spineless Prickly Pear Cactus: A Drought- and Salt-Tolerant, Selenium-Enriched Nutraceutical Fruit Crop for Biofortified Foods
journal, November 2010
- Bañuelos, Gary S.; Fakra, Sirine C.; Walse, Spencer S.
- Plant Physiology, Vol. 155, Issue 1
Interactions of selenium hyperaccumulators and nonaccumulators during cocultivation on seleniferous or nonseleniferous soil - the importance of having good neighbors
journal, January 2012
- El Mehdawi, Ali F.; Cappa, Jennifer J.; Fakra, Sirine C.
- New Phytologist, Vol. 194, Issue 1
Influence of sulfate supply on selenium uptake dynamics and expression of sulfate/selenate transporters in selenium hyperaccumulator and nonhyperaccumulator Brassicaceae
journal, October 2017
- El Mehdawi, Ali F.; Jiang, Ying; Guignardi, Zack S.
- New Phytologist, Vol. 217, Issue 1
Inoculation of selenium hyperaccumulator Stanleya pinnata and related non-accumulator Stanleya elata with hyperaccumulator rhizosphere fungi-investigation of effects on Se accumulation and speciation
journal, September 2013
- Lindblom, Stormy Dawn; Fakra, Sirine C.; Landon, Jessica
- Physiologia Plantarum, Vol. 150, Issue 1
Analysis of selenium accumulation, speciation and tolerance of potential selenium hyperaccumulator Symphyotrichum ericoides
journal, February 2014
- El Mehdawi, Ali F.; Reynolds, Ray Jason B.; Prins, Christine N.
- Physiologia Plantarum, Vol. 152, Issue 1
Selenium Hyperaccumulator Plants Stanleya pinnata and Astragalus bisulcatus Are Colonized by Se-Resistant, Se-Excluding Wasp and Beetle Seed Herbivores
journal, December 2012
- Freeman, John L.; Marcus, Matthew A.; Fakra, Sirine C.
- PLoS ONE, Vol. 7, Issue 12
Pairwise Multiple Comparison Procedures with Unequal N’s and/or Variances: A Monte Carlo Study
journal, June 1976
- Games, Paul A.; Howell, John F.
- Journal of Educational Statistics, Vol. 1, Issue 2
Characterization of selenium and sulfur accumulation across the genus Stanleya (Brassicaceae): A field survey and common-garden experiment
journal, May 2014
- Cappa, Jennifer J.; Cappa, Patrick J.; El Mehdawi, Ali F.
- American Journal of Botany, Vol. 101, Issue 5
Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae)
journal, November 2014
- Alford, Élan R.; Lindblom, Stormy D.; Pittarello, Marco
- American Journal of Botany, Vol. 101, Issue 11
ImageJ2: ImageJ for the next generation of scientific image data
preprint, January 2017
- Rueden, Curtis T.; Schindelin, Johannes; Hiner, Mark C.
- arXiv
Figures / Tables found in this record: