A Comparison of Cs-137 γ Rays and 320-kV X-Rays in a Mouse Bone Marrow Transplantation Model
Abstract
US homeland security concerns regarding the potential misuse of some radiation sources used in radiobiological research, for example, cesium-137 (137Cs), have resulted in recommendations by the National Research Council to conduct studies into replacing these sources with suitable X-ray instruments. The objective of this research is to compare the effectiveness of an X-RAD 320 irradiator (PXINC 2010) with a 137Cs irradiator (Gammacell-1000 Unit) using an established bone marrow chimeric model. Using measured radiation doses for each instrument, we characterized the dose–response relationships for bone marrow and splenocyte ablation, using a cytotoxicity-hazard model. Our results show that the X-RAD 320 photon energy spectrum was suitable for ablating bone marrow at the 3 exposure levels used, similar to that of 137Cs photons. However, the 320-kV X-rays were not as effective as the much higher energy γ rays at depleting mouse splenocytes. Furthermore, the 3 X-ray levels used were less effective than the higher energy γ rays in allowing the successful engraftment of donor bone marrow, potentially as a result of the incomplete depletion of the spleen cells. More defined studies are warranted for determining whether bone marrow transplantation in mice can be successfully achieved using 320-kV X-rays. A higher X-ray dose thenmore »
- Authors:
-
- Lovelace Respiratory Research Inst., Albuquerque, NM (United States)
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA), Office of Defense Nuclear Nonproliferation
- OSTI Identifier:
- 1618068
- Report Number(s):
- SAND-2019-15284J
Journal ID: ISSN 1559-3258; 682072; TRN: US2106789
- Grant/Contract Number:
- AC04-94AL85000; NA0003525
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Dose-Response
- Additional Journal Information:
- Journal Volume: 18; Journal Issue: 2; Journal ID: ISSN 1559-3258
- Publisher:
- International Hormesis Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; Cs-137 γ rays; 320-kV X-rays; bone marrow; spleen
Citation Formats
Gott, Katherine M., Potter, Charles A., Doyle-Eisele, Melanie, Lin, Yong, Wilder, Julie, and Scott, Bobby R. A Comparison of Cs-137 γ Rays and 320-kV X-Rays in a Mouse Bone Marrow Transplantation Model. United States: N. p., 2020.
Web. doi:10.1177/1559325820916572.
Gott, Katherine M., Potter, Charles A., Doyle-Eisele, Melanie, Lin, Yong, Wilder, Julie, & Scott, Bobby R. A Comparison of Cs-137 γ Rays and 320-kV X-Rays in a Mouse Bone Marrow Transplantation Model. United States. https://doi.org/10.1177/1559325820916572
Gott, Katherine M., Potter, Charles A., Doyle-Eisele, Melanie, Lin, Yong, Wilder, Julie, and Scott, Bobby R. Fri .
"A Comparison of Cs-137 γ Rays and 320-kV X-Rays in a Mouse Bone Marrow Transplantation Model". United States. https://doi.org/10.1177/1559325820916572. https://www.osti.gov/servlets/purl/1618068.
@article{osti_1618068,
title = {A Comparison of Cs-137 γ Rays and 320-kV X-Rays in a Mouse Bone Marrow Transplantation Model},
author = {Gott, Katherine M. and Potter, Charles A. and Doyle-Eisele, Melanie and Lin, Yong and Wilder, Julie and Scott, Bobby R.},
abstractNote = {US homeland security concerns regarding the potential misuse of some radiation sources used in radiobiological research, for example, cesium-137 (137Cs), have resulted in recommendations by the National Research Council to conduct studies into replacing these sources with suitable X-ray instruments. The objective of this research is to compare the effectiveness of an X-RAD 320 irradiator (PXINC 2010) with a 137Cs irradiator (Gammacell-1000 Unit) using an established bone marrow chimeric model. Using measured radiation doses for each instrument, we characterized the dose–response relationships for bone marrow and splenocyte ablation, using a cytotoxicity-hazard model. Our results show that the X-RAD 320 photon energy spectrum was suitable for ablating bone marrow at the 3 exposure levels used, similar to that of 137Cs photons. However, the 320-kV X-rays were not as effective as the much higher energy γ rays at depleting mouse splenocytes. Furthermore, the 3 X-ray levels used were less effective than the higher energy γ rays in allowing the successful engraftment of donor bone marrow, potentially as a result of the incomplete depletion of the spleen cells. More defined studies are warranted for determining whether bone marrow transplantation in mice can be successfully achieved using 320-kV X-rays. A higher X-ray dose then used is likely needed for transplantation success.},
doi = {10.1177/1559325820916572},
journal = {Dose-Response},
number = 2,
volume = 18,
place = {United States},
year = {Fri Apr 03 00:00:00 EDT 2020},
month = {Fri Apr 03 00:00:00 EDT 2020}
}
Web of Science
Figures / Tables:
Works referenced in this record:
Reduction of myeloid-derived suppressor cells reinforces the anti-solid tumor effect of recipient leukocyte infusion in murine neuroblastoma-bearing allogeneic bone marrow chimeras
journal, January 2018
- Dierckx de Casterlé, Isabelle; Fevery, Sabine; Rutgeerts, Omer
- Cancer Immunology, Immunotherapy, Vol. 67, Issue 4
Replacement of 137Cs Irradiators with X-ray Irradiators
journal, January 2009
- Dodd, Brian; Vetter, Richard J.
- Health Physics, Vol. 96, Issue suppl 2
Amifostine prior to lethal irradiation prevents allogeneic bone marrow engraftment in mice
journal, February 2008
- Thompson, J. S.; Asmis, R.; Chu, Y.
- Bone Marrow Transplantation, Vol. 41, Issue 11
Severe deterministic effects of external exposure and intake of radioactive material: basis for emergency response criteria
journal, May 2011
- Kutkov, V.; Buglova, E.; McKenna, T.
- Journal of Radiological Protection, Vol. 31, Issue 2
Dicentric chromosomes in monolayers of human lymphocytes produced by monochromatized synchrotron radiation with photon energies from 1.83�keV to 17.4�keV
journal, May 2004
- Krumrey, M.; Ulm, G.; Schmid, E.
- Radiation and Environmental Biophysics, Vol. 43, Issue 1
A Comparison of in vivo Cellular Responses to CS-137 Gamma Rays and 320-KV X Rays
journal, January 2013
- Scott, B. R.; Gott, K. M.; Potter, C. A.
- Dose-Response, Vol. 11, Issue 4
Establishment of Early Endpoints in Mouse Total-Body Irradiation Model
journal, August 2016
- Koch, Amory; Gulani, Jatinder; King, Gregory
- PLOS ONE, Vol. 11, Issue 8
Radiation Effectiveness Factors for use in Calculating Probability of Causation of Radiogenic Cancers
journal, January 2005
- Kocher, D. C.; Apostoaei, A. I.; Hoffman, F. O.
- Health Physics, Vol. 89, Issue 1
Targeting the Spleen as an Alternative Site for Hematopoiesis
journal, April 2019
- Short, Christie; Lim, Hong K.; Tan, Jonathan
- BioEssays, Vol. 41, Issue 5
Deletion of cIAP1 and cIAP2 in murine B lymphocytes constitutively activates cell survival pathways and inactivates the germinal center response
journal, April 2011
- Gardam, Sandra; Turner, Vivian M.; Anderton, Holly
- Blood, Vol. 117, Issue 15
Kinetics Equation Replacement Function for a Particular Continuous Intake Scenario
journal, January 2013
- Potter, Charles A.
- Health Physics, Vol. 104, Issue 1
Re-evaluation of the RBE of 29�kV x-rays (mammography x-rays) relative to 220�kV x-rays using neoplastic transformation of human CGL1-hybrid cells
journal, October 2003
- G�ggelmann, W.; Jacobsen, C.; Panzer, W.
- Radiation and Environmental Biophysics, Vol. 42, Issue 3
Characterization of an orthovoltage biological irradiator used for radiobiological research
journal, February 2015
- Azimi, Rezvan; Alaei, Parham; Spezi, Emiliano
- Journal of Radiation Research, Vol. 56, Issue 3
Comparison of X-ray vs. gamma irradiation of CPDA-1 red cells
journal, November 2005
- Janatpour, K.; Denning, L.; Nelson, K.
- Vox Sanguinis, Vol. 89, Issue 4
Evaluation of external beam hardening filters on image quality of computed tomography and single photon emission computed tomography/computed tomography
journal, January 2015
- Rana, Nivedita; Rawat, Dinesh; Parmar, Madan
- Journal of Medical Physics, Vol. 40, Issue 4
Optimal protocol for total body irradiation for allogeneic bone marrow transplantation in mice
journal, December 2002
- Cui, Y-Z; Hisha, H.; Yang, G-X
- Bone Marrow Transplantation, Vol. 30, Issue 12
Study on the repair of the radioinduced lesions involved in the formation of chromosomal aberrations in G0 human lymphocytes after exposure to γ-rays and fast neutrons
journal, May 1985
- Fabry, L.; Coton, C.
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 149, Issue 3
Analysis of chromosome aberrations in human peripheral lymphocytes induced by 5.4 keV x-rays
journal, February 1998
- Roos, H.; Schmid, E.
- Radiation and Environmental Biophysics, Vol. 36, Issue 4
Syngeneic and allogeneic bone marrow engraftment after total body irradiation: dependence on dose, dose rate, and fractionation
journal, February 1991
- Down, Jd; Tarbell, Nj; Thames, Hd
- Blood, Vol. 77, Issue 3
Stochastic Threshold Exponential (TE) Model for Hematopoietic Tissue Reconstitution Deficit after Radiation Damage
journal, January 2014
- Scott, B. R.; Potter, C. A.
- Dose-Response, Vol. 12, Issue 3
Understanding the Cellular Origin of the Mononuclear Phagocyte System Sheds Light on the Myeloid Postulate of Immune Paralysis in Sepsis
journal, April 2018
- Poulin, Lionel Franz; Lasseaux, Corentin; Chamaillard, Mathias
- Frontiers in Immunology, Vol. 9
Figures / Tables found in this record: