Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae
Abstract
Bacteria and other types of microbes interact with their hosts in several ways, including metabolic pathways, development, and complex behavioral processes such as mate recognition. During the mating season, adult males of the lesser long-nosed agave pollinator bat Leptonycteris yerbabuenae (Phyllostomidae: Glossophaginae) develop a structure called the dorsal patch, which is located in the interscapular region and may play a role in kin recognition and mate selection. Using high-throughput sequencing of the V4 region of the 16S rRNA gene, we identified a total of 2,847 microbial phylotypes in the dorsal patches of eleven specimens. Twenty-six phylotypes were shared among all the patches, accounting for 30 to 75% of their relative abundance. These shared bacteria are distributed among 13 families, 10 orders, 6 classes and 3 phyla. Two of these common bacterial components of the dorsal patch are Lactococcus and Streptococcus. Some of them—Helcococcus, Aggregatibacter, Enterococcus, and Corynebacteriaceae—include bacteria with pathogenic potential. Half of the shared phylotypes belong to Gallicola, Anaerococcus, Peptoniphilus, Proteus, Staphylococcus, Clostridium, and Peptostreptococcus and specialize in fatty acid production through fermentative processes. This work lays the basis for future symbiotic microbe studies focused on communication and reproduction strategies in wildlife.
- Authors:
-
[1];
- Univ. Nacional Autonoma de Mexico (UNAM), Mexico City (Mexico). Inst. de EcologIa; Parque Cientifico y Tecnologico de Yucatan, Merida, Yucatan (Mexico). Lab. de Ecologia Bacteriana, Inst. de Ecologia, UNAM
- Consorcio de Investigación del Golfo de México (CIGOM), Mérida, Yucatán (México). Centro de Investigación y de Estudios Avanzados del Inst. Politécnico Nacional
- Parque Cientifico y Tecnologico de Yucatan, Merida, Yucatan (Mexico). Lab. de Ecologia Bacteriana, Inst. de Ecologia, UNAM
- Univ. Nacional Autonoma de Mexico (UNAM), Morelia, Michoacán, (Mexico). Lab. de Ecología Funcional, Inst. de Investigaciones en Ecosistemas y Sustentabilidad
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Climate and Ecosystem Sciences Division; Univ. of California, Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); Soluciones Ambientales Itzeni AC; SEP-CONACyT
- OSTI Identifier:
- 1615280
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 14; Journal Issue: 12; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Gaona, Osiris, Cerqueda-García, Daniel, Falcón, Luisa I., Vázquez-Domínguez, Guillermo, Valdespino-Castillo, Patricia M., and Neri-Barrios, Carla-Ximena. Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae. United States: N. p., 2019.
Web. doi:10.1371/journal.pone.0226239.
Gaona, Osiris, Cerqueda-García, Daniel, Falcón, Luisa I., Vázquez-Domínguez, Guillermo, Valdespino-Castillo, Patricia M., & Neri-Barrios, Carla-Ximena. Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae. United States. https://doi.org/10.1371/journal.pone.0226239
Gaona, Osiris, Cerqueda-García, Daniel, Falcón, Luisa I., Vázquez-Domínguez, Guillermo, Valdespino-Castillo, Patricia M., and Neri-Barrios, Carla-Ximena. Mon .
"Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae". United States. https://doi.org/10.1371/journal.pone.0226239. https://www.osti.gov/servlets/purl/1615280.
@article{osti_1615280,
title = {Microbiota composition of the dorsal patch of reproductive male Leptonycteris yerbabuenae},
author = {Gaona, Osiris and Cerqueda-García, Daniel and Falcón, Luisa I. and Vázquez-Domínguez, Guillermo and Valdespino-Castillo, Patricia M. and Neri-Barrios, Carla-Ximena},
abstractNote = {Bacteria and other types of microbes interact with their hosts in several ways, including metabolic pathways, development, and complex behavioral processes such as mate recognition. During the mating season, adult males of the lesser long-nosed agave pollinator bat Leptonycteris yerbabuenae (Phyllostomidae: Glossophaginae) develop a structure called the dorsal patch, which is located in the interscapular region and may play a role in kin recognition and mate selection. Using high-throughput sequencing of the V4 region of the 16S rRNA gene, we identified a total of 2,847 microbial phylotypes in the dorsal patches of eleven specimens. Twenty-six phylotypes were shared among all the patches, accounting for 30 to 75% of their relative abundance. These shared bacteria are distributed among 13 families, 10 orders, 6 classes and 3 phyla. Two of these common bacterial components of the dorsal patch are Lactococcus and Streptococcus. Some of them—Helcococcus, Aggregatibacter, Enterococcus, and Corynebacteriaceae—include bacteria with pathogenic potential. Half of the shared phylotypes belong to Gallicola, Anaerococcus, Peptoniphilus, Proteus, Staphylococcus, Clostridium, and Peptostreptococcus and specialize in fatty acid production through fermentative processes. This work lays the basis for future symbiotic microbe studies focused on communication and reproduction strategies in wildlife.},
doi = {10.1371/journal.pone.0226239},
journal = {PLoS ONE},
number = 12,
volume = 14,
place = {United States},
year = {Mon Dec 16 00:00:00 EST 2019},
month = {Mon Dec 16 00:00:00 EST 2019}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 8 works
Citation information provided by
Web of Science
Web of Science
Figures / Tables:
Fig 1: Development of dorsal patch in L. yerbabuenae. The dorsal areas of different male bats captured during the same sampling period show patches at different stages of development from a less developed patch (left) to a mature, well-developed patch (right).
All figures and tables
(10 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Gut microbiome composition is associated with temperament during early childhood
journal, March 2015
- Christian, Lisa M.; Galley, Jeffrey D.; Hade, Erinn M.
- Brain, Behavior, and Immunity, Vol. 45
UCHIME improves sensitivity and speed of chimera detection
journal, June 2011
- Edgar, Robert C.; Haas, Brian J.; Clemente, Jose C.
- Bioinformatics, Vol. 27, Issue 16
Seasonal distribution of the long-nosed bat ( Leptonycteris curasoae ) in North America: does a generalized migration pattern really exist?
journal, September 1999
- Rojas-Martínez, Alberto; Valiente-Banuet, Alfonso; del Coro Arizmendi, María
- Journal of Biogeography, Vol. 26, Issue 5
Dorsal Patch and Chemical Signaling in Males of the Long-Nosed Bat, Leptonycteris curasoae (Chiroptera: Phyllostomidae)
journal, October 2009
- Muñoz-Romo, Mariana; Kunz, Thomas H.
- Journal of Mammalogy, Vol. 90, Issue 5
Social behavior and the microbiome
journal, December 2015
- Archie, Elizabeth A.; Tung, Jenny
- Current Opinion in Behavioral Sciences, Vol. 6
Cospeciation of gut microbiota with hominids
journal, July 2016
- Moeller, A. H.; Caro-Quintero, A.; Mjungu, D.
- Science, Vol. 353, Issue 6297
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
journal, March 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- Systematic Biology, Vol. 59, Issue 3
The vocabulary of microbiome research: a proposal
journal, July 2015
- Marchesi, Julian R.; Ravel, Jacques
- Microbiome, Vol. 3, Issue 1
Animal behaviour meets microbial ecology
journal, September 2011
- Archie, Elizabeth A.; Theis, Kevin R.
- Animal Behaviour, Vol. 82, Issue 3
An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea
journal, December 2011
- McDonald, Daniel; Price, Morgan N.; Goodrich, Julia
- The ISME Journal, Vol. 6, Issue 3
Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus.
journal, July 2001
- Ezaki, T.; Kawamura, Y.; Li, N.
- International Journal of Systematic and Evolutionary Microbiology, Vol. 51, Issue 4
Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing
journal, December 2012
- Bokulich, Nicholas A.; Subramanian, Sathish; Faith, Jeremiah J.
- Nature Methods, Vol. 10, Issue 1
Tracking the origin of the smearing behavior in long-nosed bats (Leptonycteris spp.)
journal, November 2016
- Nassar, Jafet M.; Galicia, Rubén; Ibarra, Ana
- Mammalian Biology, Vol. 81, Issue 6
Has the Microbiota Played a Critical Role in the Evolution of the Adaptive Immune System?
journal, December 2010
- Lee, Y. K.; Mazmanian, S. K.
- Science, Vol. 330, Issue 6012
Seasonal sebaceous patch in the nectar-feeding bats Leptonycteris curasoae and L. yerbabuenae (Phyllostomidae: Glossophaginae): phenological, histological, and preliminary chemical characterization
journal, August 2008
- Nassar, Jafet M.; Salazar, M. Víctor; Quintero, Alberto
- Zoology, Vol. 111, Issue 5
Reclassification of Peptostreptococcus magnus (Prevot 1933) Holdeman and Moore 1972 as Finegoldia magna comb. nov. and Peptostreptococcus micros (Prevot 1933) Smith 1957 as Micromonas micros comb. nov.
journal, October 1999
- Murdoch, D. A.; Shah, H. N.
- Anaerobe, Vol. 5, Issue 5
Worlds within worlds: evolution of the vertebrate gut microbiota
journal, October 2008
- Ley, Ruth E.; Lozupone, Catherine A.; Hamady, Micah
- Nature Reviews Microbiology, Vol. 6, Issue 10
Evidences on the migratory movements of the nectar-feeding bat Leptonycteris curasoae in Mexico using random amplified polymorphic DNA (RAPD)
journal, January 2007
- Morales-Garza, M. R.; Arizmendi, M. del C.; Campos, J. E.
- Journal of Arid Environments, Vol. 68, Issue 2
QIIME allows analysis of high-throughput community sequencing data
journal, April 2010
- Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
- Nature Methods, Vol. 7, Issue 5
Microbial degradation of complex carbohydrates in the gut
journal, July 2012
- Flint, Harry J.; Scott, Karen P.; Duncan, Sylvia H.
- Gut Microbes, Vol. 3, Issue 4
Wild canid associations with fermentation products
journal, December 1982
- Bullard, Roger W.
- Industrial & Engineering Chemistry Product Research and Development, Vol. 21, Issue 4
Symbiotic bacteria appear to mediate hyena social odors
journal, November 2013
- Theis, K. R.; Venkataraman, A.; Dycus, J. A.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 49
Flavour formation by lactic acid bacteria and biochemical flavour profiling of cheese products
journal, August 2005
- Smit, Gerrit; Smit, Bart A.; Engels, Wim J. M.
- FEMS Microbiology Reviews, Vol. 29, Issue 3
MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004
- Edgar, R. C.
- Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
FLASH: fast length adjustment of short reads to improve genome assemblies
journal, September 2011
- Magoc, T.; Salzberg, S. L.
- Bioinformatics, Vol. 27, Issue 21
Disruptive physiology: olfaction and the microbiome-gut-brain axis: Olfaction and gut-brain axis
journal, July 2017
- Bienenstock, John; Kunze, Wolfgang A.; Forsythe, Paul
- Biological Reviews, Vol. 93, Issue 1
Towards an Evolutionary Model of Animal-Associated Microbiomes
journal, February 2011
- Yeoman, Carl J.; Chia, Nicholas; Yildirim, Suleyman
- Entropy, Vol. 13, Issue 3
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012
- Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
- The ISME Journal, Vol. 6, Issue 8
Gut microbiota and kin recognition
journal, June 2013
- Lizé, Anne; McKay, Raegan; Lewis, Zenobia
- Trends in Ecology & Evolution, Vol. 28, Issue 6
Search and clustering orders of magnitude faster than BLAST
journal, August 2010
- Edgar, Robert C.
- Bioinformatics, Vol. 26, Issue 19, p. 2460-2461
phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data
journal, April 2013
- McMurdie, Paul J.; Holmes, Susan
- PLoS ONE, Vol. 8, Issue 4
The anal sac secretion of the red fox (Vulpes vulpes); its chemistry and microbiology. A comparison with the anal sac secretion of the lion (Panthera leo)
journal, January 1974
- Albone, Eric S.; Eglinton, Geoffrey; Walker, Jean M.
- Life Sciences, Vol. 14, Issue 2
Microbial community structure and functional properties in permanently and seasonally flooded areas in Poyang Lake
journal, March 2020
- Liu, Yang; Ren, Ze; Qu, Xiaodong
- Scientific Reports, Vol. 10, Issue 1
Fungal diversity driven by bark features affects phorophyte preference in epiphytic orchids from southern China
journal, May 2021
- Pecoraro, Lorenzo; Rasmussen, Hanne N.; Gomes, Sofia I. F.
- Scientific Reports, Vol. 11, Issue 1
New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0
text, January 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- Oxford University Press
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
text, January 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- ETH Zurich
Reclassification of Peptostreptococcus magnus (Prevot 1933) Holdeman and Moore 1972 as Finegoldia magna comb. nov. and Peptostreptococcus micros (Prevot 1933) Smith 1957 as Micromonas micros comb. nov.
journal, October 1999
- Murdoch, D. A.; Shah, H. N.
- Anaerobe, Vol. 5, Issue 5
The anal sac secretion of the red fox (Vulpes vulpes); its chemistry and microbiology. A comparison with the anal sac secretion of the lion (Panthera leo)
journal, January 1974
- Albone, Eric S.; Eglinton, Geoffrey; Walker, Jean M.
- Life Sciences, Vol. 14, Issue 2
Animal behaviour meets microbial ecology
journal, September 2011
- Archie, Elizabeth A.; Theis, Kevin R.
- Animal Behaviour, Vol. 82, Issue 3
Gut microbiome composition is associated with temperament during early childhood
journal, March 2015
- Christian, Lisa M.; Galley, Jeffrey D.; Hade, Erinn M.
- Brain, Behavior, and Immunity, Vol. 45
Social behavior and the microbiome
journal, December 2015
- Archie, Elizabeth A.; Tung, Jenny
- Current Opinion in Behavioral Sciences, Vol. 6
Evidences on the migratory movements of the nectar-feeding bat Leptonycteris curasoae in Mexico using random amplified polymorphic DNA (RAPD)
journal, January 2007
- Morales-Garza, M. R.; Arizmendi, M. del C.; Campos, J. E.
- Journal of Arid Environments, Vol. 68, Issue 2
Gut microbiota and kin recognition
journal, June 2013
- Lizé, Anne; McKay, Raegan; Lewis, Zenobia
- Trends in Ecology & Evolution, Vol. 28, Issue 6
Seasonal sebaceous patch in the nectar-feeding bats Leptonycteris curasoae and L. yerbabuenae (Phyllostomidae: Glossophaginae): phenological, histological, and preliminary chemical characterization
journal, August 2008
- Nassar, Jafet M.; Salazar, M. Víctor; Quintero, Alberto
- Zoology, Vol. 111, Issue 5
Wild canid associations with fermentation products
journal, December 1982
- Bullard, Roger W.
- Industrial & Engineering Chemistry Product Research and Development, Vol. 21, Issue 4
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012
- Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
- The ISME Journal, Vol. 6, Issue 8
Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing
journal, December 2012
- Bokulich, Nicholas A.; Subramanian, Sathish; Faith, Jeremiah J.
- Nature Methods, Vol. 10, Issue 1
Worlds within worlds: evolution of the vertebrate gut microbiota
journal, October 2008
- Ley, Ruth E.; Lozupone, Catherine A.; Hamady, Micah
- Nature Reviews Microbiology, Vol. 6, Issue 10
Microbiota-accessible carbohydrates suppress Clostridium difficile infection in a murine model
journal, April 2018
- Hryckowian, Andrew J.; Van Treuren, William; Smits, Samuel A.
- Nature Microbiology, Vol. 3, Issue 6
Horizontal gene transfer enables programmable gene stability in synthetic microbiota
journal, September 2022
- Wang, Teng; Weiss, Andrea; Aqeel, Ammara
- Nature Chemical Biology, Vol. 18, Issue 11
Characterizing the scent and chemical composition of Panthera leo marking fluid using solid-phase microextraction and multidimensional gas chromatography–mass spectrometry-olfactometry
journal, July 2017
- Soso, Simone B.; Koziel, Jacek A.
- Scientific Reports, Vol. 7, Issue 1
Infant gut microbiota characteristics generally do not modify effects of lipid-based nutrient supplementation on growth or inflammation: secondary analysis of a randomized controlled trial in Malawi
journal, September 2020
- Hughes, Riley L.; Arnold, Charles D.; Young, Rebecca R.
- Scientific Reports, Vol. 10, Issue 1
Seasonal distribution of the long-nosed bat ( Leptonycteris curasoae ) in North America: does a generalized migration pattern really exist?
journal, September 1999
- Rojas-Martínez, Alberto; Valiente-Banuet, Alfonso; del Coro Arizmendi, María
- Journal of Biogeography, Vol. 26, Issue 5
Symbiotic bacteria appear to mediate hyena social odors
journal, November 2013
- Theis, K. R.; Venkataraman, A.; Dycus, J. A.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 49
Search and clustering orders of magnitude faster than BLAST
journal, August 2010
- Edgar, Robert C.
- Bioinformatics, Vol. 26, Issue 19, p. 2460-2461
UCHIME improves sensitivity and speed of chimera detection
journal, June 2011
- Edgar, Robert C.; Haas, Brian J.; Clemente, Jose C.
- Bioinformatics, Vol. 27, Issue 16
MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004
- Edgar, R. C.
- Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0
journal, March 2010
- Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent
- Systematic Biology, Vol. 59, Issue 3
Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus.
journal, July 2001
- Ezaki, T.; Kawamura, Y.; Li, N.
- International Journal of Systematic and Evolutionary Microbiology, Vol. 51, Issue 4
Has the Microbiota Played a Critical Role in the Evolution of the Adaptive Immune System?
journal, December 2010
- Lee, Y. K.; Mazmanian, S. K.
- Science, Vol. 330, Issue 6012
Cospeciation of gut microbiota with hominids
journal, July 2016
- Moeller, A. H.; Caro-Quintero, A.; Mjungu, D.
- Science, Vol. 353, Issue 6297
Helcococcus, a new genus of catalase-negative, gram-positive cocci isolated from clinical specimens
journal, June 1995
- Caliendo, A. M.; Jordan, C. D.; Ruoff, K. L.
- Journal of Clinical Microbiology, Vol. 33, Issue 6
Role of the Gut Microbiome in Vertebrate Evolution
journal, March 2018
- Sharpton, Thomas J.
- mSystems, Vol. 3, Issue 2
Smearing behaviour of male Leptonycteris curasoae (Chiroptera) and female responses to the odour of dorsal patches
journal, January 2011
- Kunz, Thomas; Burgos, Juan; Muñoz-Romo, Mariana
- Behaviour, Vol. 148, Issue 4
The vocabulary of microbiome research: a proposal
journal, July 2015
- Marchesi, Julian R.; Ravel, Jacques
- Microbiome, Vol. 3, Issue 1
Host immunoglobulin G selectively identifies pathobionts in pediatric inflammatory bowel diseases
journal, January 2019
- Armstrong, Heather; Alipour, Misagh; Valcheva, Rosica
- Microbiome, Vol. 7, Issue 1
Reproductive Endocrinology of a Small Tropical Bat (Female Saccopteryx bilineata; Emballonuridae) Monitored by Fecal Hormone Metabolites
journal, February 2008
- Voigt, Christian C.; Schwarzenberger, Franz
- Journal of Mammalogy, Vol. 89, Issue 1
Guidelines of the American Society of Mammalogists for the Use of Wild Mammals in Research
journal, June 2007
- Gannon, William L.; Sikes, Robert S.
- Journal of Mammalogy, Vol. 88, Issue 3
Internal and external indicators of male reproduction in the lesser long-nosed bat Leptonycteris yerbabuenae
journal, April 2013
- Rincón-Vargas, Felipe; Stoner, Kathryn E.; Vigueras-Villaseñor, Rosa María
- Journal of Mammalogy, Vol. 94, Issue 2
Bats, Bacteria, and bat Smell: Sex-Specific Diversity of Microbes in a Sexually Selected Scent Organ
journal, August 2005
- Voigt, Christian C.; Caspers, Barbara; Speck, Stephanie
- Journal of Mammalogy, Vol. 86, Issue 4
Chemical Composition of the Substances from Dorsal Patches of Males of the CuraÇAoan Long-Nosed Bat, Leptonycteris curasoae (Phyllostomidae: Glossophaginae)
journal, June 2012
- Muñoz-Romo, Mariana; Nielsen, Lawrence T.; Nassar, Jafet M.
- Acta Chiropterologica, Vol. 14, Issue 1
Evolutionary Biology Needs Wild Microbiomes
journal, April 2017
- Hird, Sarah M.
- Frontiers in Microbiology, Vol. 8
Towards an Evolutionary Model of Animal-Associated Microbiomes
journal, February 2011
- Yeoman, Carl J.; Chia, Nicholas; Yildirim, Suleyman
- Entropy, Vol. 13, Issue 3
Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.