Mosquito-Borne Viruses and Insect-Specific Viruses Revealed in Field-Collected Mosquitoes by a Monitoring Tool Adapted from a Microbial Detection Array
Abstract
Several mosquito-borne diseases affecting humans are emerging or reemerging in the United States. The early detection of pathogens in mosquito populations is essential to prevent and control the spread of these diseases. Here, we tested the potential applicability of the Lawrence Livermore Microbial Detection Array (LLMDA) to enhance biosurveillance by detecting microbes present in Aedes aegypti, Aedes albopictus, and Culex mosquitoes, which are major vector species globally, including in Texas. The sensitivity and reproducibility of the LLMDA were tested in mosquito samples spiked with different concentrations of dengue virus (DENV), revealing a detection limit of >100 but <1,000 PFU/ml. Additionally, field-collected mosquitoes from Chicago, IL, and College Station, TX, of known infection status (West Nile virus [WNV] and Culex flavivirus [CxFLAV] positive) were tested on the LLMDA to confirm its efficiency. Mosquito field samples of unknown infection status, collected in San Antonio, TX, and the Lower Rio Grande Valley (LRGV), TX, were run on the LLMDA and further confirmed by PCR or quantitative PCR (qPCR). The analysis of the field samples with the LLMDA revealed the presence of cell-fusing agent virus (CFAV) in A. aegypti populations. Wolbachia was also detected in several of the field samples (A. albopictus and Culexmore »
- Authors:
-
- Texas A & M Univ., College Station, TX (United States)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Texas A & M Univ., San Antonio, TX (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
- OSTI Identifier:
- 1614968
- Report Number(s):
- LLNL-JRNL-750498
Journal ID: ISSN 0099-2240; 935708
- Grant/Contract Number:
- AC52-07NA27344; R21AI128953
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied and Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 85; Journal Issue: 19; Journal ID: ISSN 0099-2240
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
Citation Formats
Martin, Estelle, Borucki, Monica K., Thissen, James, Garcia-Luna, Selene, Hwang, Mona, Wise de Valdez, Megan, Jaing, Crystal J., Hamer, Gabriel L., and Frank, Matthias. Mosquito-Borne Viruses and Insect-Specific Viruses Revealed in Field-Collected Mosquitoes by a Monitoring Tool Adapted from a Microbial Detection Array. United States: N. p., 2019.
Web. doi:10.1128/AEM.01202-19.
Martin, Estelle, Borucki, Monica K., Thissen, James, Garcia-Luna, Selene, Hwang, Mona, Wise de Valdez, Megan, Jaing, Crystal J., Hamer, Gabriel L., & Frank, Matthias. Mosquito-Borne Viruses and Insect-Specific Viruses Revealed in Field-Collected Mosquitoes by a Monitoring Tool Adapted from a Microbial Detection Array. United States. https://doi.org/10.1128/AEM.01202-19
Martin, Estelle, Borucki, Monica K., Thissen, James, Garcia-Luna, Selene, Hwang, Mona, Wise de Valdez, Megan, Jaing, Crystal J., Hamer, Gabriel L., and Frank, Matthias. Fri .
"Mosquito-Borne Viruses and Insect-Specific Viruses Revealed in Field-Collected Mosquitoes by a Monitoring Tool Adapted from a Microbial Detection Array". United States. https://doi.org/10.1128/AEM.01202-19. https://www.osti.gov/servlets/purl/1614968.
@article{osti_1614968,
title = {Mosquito-Borne Viruses and Insect-Specific Viruses Revealed in Field-Collected Mosquitoes by a Monitoring Tool Adapted from a Microbial Detection Array},
author = {Martin, Estelle and Borucki, Monica K. and Thissen, James and Garcia-Luna, Selene and Hwang, Mona and Wise de Valdez, Megan and Jaing, Crystal J. and Hamer, Gabriel L. and Frank, Matthias},
abstractNote = {Several mosquito-borne diseases affecting humans are emerging or reemerging in the United States. The early detection of pathogens in mosquito populations is essential to prevent and control the spread of these diseases. Here, we tested the potential applicability of the Lawrence Livermore Microbial Detection Array (LLMDA) to enhance biosurveillance by detecting microbes present in Aedes aegypti, Aedes albopictus, and Culex mosquitoes, which are major vector species globally, including in Texas. The sensitivity and reproducibility of the LLMDA were tested in mosquito samples spiked with different concentrations of dengue virus (DENV), revealing a detection limit of >100 but <1,000 PFU/ml. Additionally, field-collected mosquitoes from Chicago, IL, and College Station, TX, of known infection status (West Nile virus [WNV] and Culex flavivirus [CxFLAV] positive) were tested on the LLMDA to confirm its efficiency. Mosquito field samples of unknown infection status, collected in San Antonio, TX, and the Lower Rio Grande Valley (LRGV), TX, were run on the LLMDA and further confirmed by PCR or quantitative PCR (qPCR). The analysis of the field samples with the LLMDA revealed the presence of cell-fusing agent virus (CFAV) in A. aegypti populations. Wolbachia was also detected in several of the field samples (A. albopictus and Culex spp.) by the LLMDA. Our findings demonstrated that the LLMDA can be used to detect multiple arboviruses of public health importance, including viruses that belong to the Flavivirus, Alphavirus, and Orthobunyavirus genera. Additionally, insect-specific viruses and bacteria were also detected in field-collected mosquitoes. Another strength of this array is its ability to detect multiple viruses in the same mosquito pool, allowing for the detection of cocirculating pathogens in an area and the identification of potential ecological associations between different viruses. Finally, this array can aid in the biosurveillance of mosquito-borne viruses circulating in specific geographical areas.},
doi = {10.1128/AEM.01202-19},
journal = {Applied and Environmental Microbiology},
number = 19,
volume = 85,
place = {United States},
year = {Fri Jul 26 00:00:00 EDT 2019},
month = {Fri Jul 26 00:00:00 EDT 2019}
}
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Cited by: 7 works
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Figures / Tables:
Table 1: LLMDA limit of detection and reproducibility in spiked mosquito pools
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