Impact of protein blocking on enzymatic saccharification of bagasse from sugarcane clones

Ázar, Rafaela I.  S.  Ladeira; Morgan, Túlio; Barbosa, Márcio H.  P.; Guimarães, Valéria M.; Ximenes, Eduardo; Ladisch, Michael

doi:10.1002/bit.26962

Title: Impact of protein blocking on enzymatic saccharification of bagasse from sugarcane clones

Abstract

Lignin plays an important functional and structural role in plants, but also contributes to the recalcitrance of lignocellulosic biomass to hydrolysis. This study addresses the influence of lignin in hydrolysis of sugarcane bagasse from conventional bred lines (UFV260 and UFV204) that were selected from 432 field‐grown clones. In addition to higher sugar production, bagasse clone UFV204 had a small, but statistically significant, lower insoluble lignin content compared with clone UFV260 (15.5% vs, 16.6%) and also exhibited a significantly higher cellulose conversion to glucose (81.3% vs. 63.3%) at a cellulase loading of 5 (filter paper unit) FPU/g of glucan or 3 FPU/g total solids for liquid hot water pretreated bagasse (200°C, 10 min). The enzyme loading was further decreased by 50% to 2.5 FPU/g glucan and resulted in a similar glucan conversion (88.5%) for clone UFV204 when the bagasse was preincubated with bovine serum albumin at pH 4.8 and nonproductive binding of cellulase components was blocked. Comparison of Langmuir adsorption isotherms and differential adsorption of the three major cellulolytic enzyme components endoglucanase, cellobiohydrolase, and β‐glucosidase help to explain differences due to lignin content.

Authors:

Ázar, Rafaela I. S. Ladeira ^[1]; Morgan, Túlio ^[2]; Barbosa, Márcio H. P. ^[2];

^[2]; Ximenes, Eduardo ^[3];

^[3]

Purdue Univ., West Lafayette, IN (United States). Lab. of Renewable Resources Engineering; Federal Univ. of Viçosa (Brazil)
Federal Univ. of Viçosa (Brazil)
Purdue Univ., West Lafayette, IN (United States). Lab. of Renewable Resources Engineering

Publication Date:: Thu Mar 21 00:00:00 EDT 2019

Research Org.:: Purdue Univ., West Lafayette, IN (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

OSTI Identifier:: 1613510

Alternate Identifier(s):: OSTI ID: 1994399

Grant/Contract Number:: EE0008256

Resource Type:: Accepted Manuscript

Journal Name:: Biotechnology and Bioengineering

Additional Journal Information:: Journal Volume: 116; Journal Issue: 7; Journal ID: ISSN 0006-3592

Publisher:: Wiley

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Biotechnology & applied microbiology; adsorption isotherms; bagasse clones; bovine serum albumin; enzymatic hydrolysis; lignin

Citation Formats


                    Ázar, Rafaela I.  S.  Ladeira, Morgan, Túlio, Barbosa, Márcio H.  P., Guimarães, Valéria M., Ximenes, Eduardo, and Ladisch, Michael. Impact of protein blocking on enzymatic saccharification of bagasse from sugarcane clones.  United States: N. p., 2019. 
Web.  doi:10.1002/bit.26962.

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                    Ázar, Rafaela I.  S.  Ladeira, Morgan, Túlio, Barbosa, Márcio H.  P., Guimarães, Valéria M., Ximenes, Eduardo, & Ladisch, Michael. Impact of protein blocking on enzymatic saccharification of bagasse from sugarcane clones.  United States.  https://doi.org/10.1002/bit.26962

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                    Ázar, Rafaela I.  S.  Ladeira, Morgan, Túlio, Barbosa, Márcio H.  P., Guimarães, Valéria M., Ximenes, Eduardo, and Ladisch, Michael. Thu .  
"Impact of protein blocking on enzymatic saccharification of bagasse from sugarcane clones".  United States.  https://doi.org/10.1002/bit.26962.  https://www.osti.gov/servlets/purl/1613510.

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@article{osti_1613510,

  title        = {Impact of protein blocking on enzymatic saccharification of bagasse from sugarcane clones},

  author       = {Ázar, Rafaela I.  S.  Ladeira and Morgan, Túlio and Barbosa, Márcio H.  P. and Guimarães, Valéria M. and Ximenes, Eduardo and Ladisch, Michael},

  abstractNote = {Lignin plays an important functional and structural role in plants, but also contributes to the recalcitrance of lignocellulosic biomass to hydrolysis. This study addresses the influence of lignin in hydrolysis of sugarcane bagasse from conventional bred lines (UFV260 and UFV204) that were selected from 432 field‐grown clones. In addition to higher sugar production, bagasse clone UFV204 had a small, but statistically significant, lower insoluble lignin content compared with clone UFV260 (15.5% vs, 16.6%) and also exhibited a significantly higher cellulose conversion to glucose (81.3% vs. 63.3%) at a cellulase loading of 5 (filter paper unit) FPU/g of glucan or 3 FPU/g total solids for liquid hot water pretreated bagasse (200°C, 10 min). The enzyme loading was further decreased by 50% to 2.5 FPU/g glucan and resulted in a similar glucan conversion (88.5%) for clone UFV204 when the bagasse was preincubated with bovine serum albumin at pH 4.8 and nonproductive binding of cellulase components was blocked. Comparison of Langmuir adsorption isotherms and differential adsorption of the three major cellulolytic enzyme components endoglucanase, cellobiohydrolase, and β‐glucosidase help to explain differences due to lignin content.},

  doi          = {10.1002/bit.26962},

  journal      = {Biotechnology and Bioengineering},

  number       = 7,

  volume       = 116,

  place        = {United States},

  year         = {Thu Mar 21 00:00:00 EDT 2019},

  month        = {Thu Mar 21 00:00:00 EDT 2019}

}

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Works referencing / citing this record:

Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse
journal, January 2020

Ladeira Ázar, Rafaela I. S.; Bordignon-Junior, Sidnei Emilio; Laufer, Craig
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Title: Impact of protein blocking on enzymatic saccharification of bagasse from sugarcane clones

Abstract

Citation Formats

Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass journal, April 2011

Lignin monomer composition affects Arabidopsis cell-wall degradability after liquid hot water pretreatment journal, January 2010

Pretreatment: The key to efficient utilization of lignocellulosic materials journal, November 2012

Bioconversion of lignocellulose: inhibitors and detoxification journal, January 2013

Deactivation and activation of lignocellulose degrading enzymes in the presence of laccase journal, February 2018

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Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects journal, January 2016

Liquid Hot Water Pretreatment of Cellulosic Biomass book, January 2009

Lignin modification improves fermentable sugar yields for biofuel production journal, June 2007

The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility journal, January 2012

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar journal, March 1959

Severity factor coefficients for subcritical liquid hot water pretreatment of hardwood chips: Severity Factor for Liquid Hot Water Pretreatment journal, August 2013

Access of cellulase to cellulose and lignin for poplar solids produced by leading pretreatment technologies journal, May 2009

Effect of liquid hot water pretreatment severity on properties of hardwood lignin and enzymatic hydrolysis of cellulose: Lignin Effect on Enzyme Hydrolysis of Cellulose journal, October 2014

Hydrothermal processing of lignocellulosic materials journal, June 1999

Sugarcane bagasse—The future composite material: A literature review journal, June 2013

Hydrolysis-determining substrate characteristics in liquid hot water pretreated hardwood: Hydrolysis-Determining Substrate Characteristics journal, January 2015

Impact of Liquid Hot Water Pretreatment on the Structural Changes of Sugarcane Bagasse Biomass for Sugar Production journal, January 2014

Path Analysis for Selection of Saccharification-Efficient Sugarcane Genotypes through Agronomic Traits journal, September 2014

Nonlinear least-squares data fitting in Excel spreadsheets journal, January 2010

Chemical and morphological characterization of sugarcane bagasse submitted to a delignification process for enhanced enzymatic digestibility journal, January 2011

Genetic improvement of sugar cane for bioenergy: the brazilian experience in network research with RIDESA journal, December 2012

The influence of pretreatment methods on saccharification of sugarcane bagasse by an enzyme extract from Chrysoporthe cubensis and commercial cocktails: A comparative study journal, September 2015

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Enhancement of enzymatic saccharification of sugarcane bagasse by liquid hot water pretreatment journal, September 2013

Advances in modifying lignin for enhanced biofuel production journal, June 2010

Lignin–Enzyme Interactions in the Hydrolysis of Lignocellulosic Biomass journal, May 2019

Correlation of Glucose (Dextrose) Degradation at 90 to 190°C in 0.4 to 20% acid journal, March 1987

Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism journal, January 2014

Wheat Straw Autohydrolysis: Process Optimization and Products Characterization journal, December 2008

Deactivation of cellulases by phenols journal, January 2011

Measurement of cellulase activities journal, January 1987

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant journal, March 2014

Evaluation of lime and hydrothermal pretreatments for efficient enzymatic hydrolysis of raw sugarcane bagasse journal, December 2015

Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities journal, January 2016

The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis journal, January 2010

Soybean protein as a cost-effective lignin-blocking additive for the saccharification of sugarcane bagasse journal, December 2016

Lignin depolymerization/repolymerization and its critical role for delignification of aspen wood by steam explosion journal, November 2007

Production of bioethanol from sugarcane bagasse: Status and perspectives journal, July 2010

Predicting Enzyme Adsorption to Lignin Films by Calculating Enzyme Surface Hydrophobicity journal, May 2014

Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation processes: Enzyme production for sugarcane bagasse hydrolysis journal, August 2016

Adsorption of enzyme onto lignins of liquid hot water pretreated hardwoods: Enzyme Adsorption by Lignin in Hardwood journal, September 2014

Inhibition of cellulases by phenols journal, March 2010

Microscopic examination of changes of plant cell structure in corn stover due to hot water pretreatment and enzymatic hydrolysis journal, January 2007

Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse journal, January 2020

Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass
journal, April 2011

Lignin monomer composition affects Arabidopsis cell-wall degradability after liquid hot water pretreatment
journal, January 2010

Pretreatment: The key to efficient utilization of lignocellulosic materials
journal, November 2012

Bioconversion of lignocellulose: inhibitors and detoxification
journal, January 2013

Deactivation and activation of lignocellulose degrading enzymes in the presence of laccase
journal, February 2018

Loosening lignin's grip on biofuel production
journal, July 2007

Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects
journal, January 2016

Liquid Hot Water Pretreatment of Cellulosic Biomass
book, January 2009

Lignin modification improves fermentable sugar yields for biofuel production
journal, June 2007

The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility
journal, January 2012

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
journal, March 1959

Severity factor coefficients for subcritical liquid hot water pretreatment of hardwood chips: Severity Factor for Liquid Hot Water Pretreatment
journal, August 2013

Access of cellulase to cellulose and lignin for poplar solids produced by leading pretreatment technologies
journal, May 2009

Effect of liquid hot water pretreatment severity on properties of hardwood lignin and enzymatic hydrolysis of cellulose: Lignin Effect on Enzyme Hydrolysis of Cellulose
journal, October 2014

Hydrothermal processing of lignocellulosic materials
journal, June 1999

Sugarcane bagasse—The future composite material: A literature review
journal, June 2013

Hydrolysis-determining substrate characteristics in liquid hot water pretreated hardwood: Hydrolysis-Determining Substrate Characteristics
journal, January 2015

Impact of Liquid Hot Water Pretreatment on the Structural Changes of Sugarcane Bagasse Biomass for Sugar Production
journal, January 2014

Path Analysis for Selection of Saccharification-Efficient Sugarcane Genotypes through Agronomic Traits
journal, September 2014

Nonlinear least-squares data fitting in Excel spreadsheets
journal, January 2010

Chemical and morphological characterization of sugarcane bagasse submitted to a delignification process for enhanced enzymatic digestibility
journal, January 2011

Genetic improvement of sugar cane for bioenergy: the brazilian experience in network research with RIDESA
journal, December 2012

The influence of pretreatment methods on saccharification of sugarcane bagasse by an enzyme extract from Chrysoporthe cubensis and commercial cocktails: A comparative study
journal, September 2015

Recycling Cellulases during the Hydrolysis of Steam Exploded and Ethanol Pretreated Lodgepole Pine
journal, August 2007

Enhancement of enzymatic saccharification of sugarcane bagasse by liquid hot water pretreatment
journal, September 2013

Advances in modifying lignin for enhanced biofuel production
journal, June 2010

Lignin–Enzyme Interactions in the Hydrolysis of Lignocellulosic Biomass
journal, May 2019

Correlation of Glucose (Dextrose) Degradation at 90 to 190°C in 0.4 to 20% acid
journal, March 1987

Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism
journal, January 2014

Wheat Straw Autohydrolysis: Process Optimization and Products Characterization
journal, December 2008

Deactivation of cellulases by phenols
journal, January 2011

Measurement of cellulase activities
journal, January 1987

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014

Evaluation of lime and hydrothermal pretreatments for efficient enzymatic hydrolysis of raw sugarcane bagasse
journal, December 2015

Effect of phenolic compounds from pretreated sugarcane bagasse on cellulolytic and hemicellulolytic activities
journal, January 2016

The effect of isolated lignins, obtained from a range of pretreated lignocellulosic substrates, on enzymatic hydrolysis
journal, January 2010

Soybean protein as a cost-effective lignin-blocking additive for the saccharification of sugarcane bagasse
journal, December 2016

Lignin depolymerization/repolymerization and its critical role for delignification of aspen wood by steam explosion
journal, November 2007

Production of bioethanol from sugarcane bagasse: Status and perspectives
journal, July 2010

Predicting Enzyme Adsorption to Lignin Films by Calculating Enzyme Surface Hydrophobicity
journal, May 2014

Secretome analysis of Trichoderma reesei and Aspergillus niger cultivated by submerged and sequential fermentation processes: Enzyme production for sugarcane bagasse hydrolysis
journal, August 2016

Adsorption of enzyme onto lignins of liquid hot water pretreated hardwoods: Enzyme Adsorption by Lignin in Hardwood
journal, September 2014

Inhibition of cellulases by phenols
journal, March 2010

Microscopic examination of changes of plant cell structure in corn stover due to hot water pretreatment and enzymatic hydrolysis
journal, January 2007

Effect of Lignin Content on Cellulolytic Saccharification of Liquid Hot Water Pretreated Sugarcane Bagasse
journal, January 2020