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Title: Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures

Abstract

Radiation damage limits the accuracy of macromolecular structures in X-ray crystallography. Cryogenic (cryo-) cooling reduces the global radiation damage rate and, therefore, became the method of choice over the past decades. The recent advent of serial crystallography, which spreads the absorbed energy over many crystals, thereby reducing damage, has rendered room temperature (RT) data collection more practical and also extendable to microcrystals, both enabling and requiring the study of specific and global radiation damage at RT. Here, we performed sequential serial raster-scanning crystallography using a microfocused synchrotron beam that allowed for the collection of two series of 40 and 90 full datasets at 2- and 1.9-Å resolution at a dose rate of 40.3 MGy/s on hen egg white lysozyme (HEWL) crystals at RT and cryotemperature, respectively. The diffraction intensity halved its initial value at average doses (D1/2) of 0.57 and 15.3 MGy at RT and 100 K, respectively. Specific radiation damage at RT was observed at disulfide bonds but not at acidic residues, increasing and then apparently reversing, a peculiar behavior that can be modeled by accounting for differential diffraction intensity decay due to the nonuniform illumination by the X-ray beam. Specific damage to disulfide bonds is evident early onmore » at RT and proceeds at a fivefold higher rate than global damage. The decay modeling suggests it is advisable not to exceed a dose of 0.38 MGy per dataset in static and time-resolved synchrotron crystallography experiments at RT. This rough yardstick might change for proteins other than HEWL and at resolutions other than 2 Å.« less

Authors:
ORCiD logo [1];  [2];  [3];  [4]; ORCiD logo [5];  [6];  [3];  [4];  [1]; ORCiD logo [1]
+ Show Author Affiliations
  1. Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), Grenoble (France); Univ. of Grenoble (France); Centre National de la Recherche Scientifique (CNRS), Grenoble (France). Inst. de Biologie Structurale (IBS)
  2. Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), Grenoble (France); Univ. of Grenoble (France); Centre National de la Recherche Scientifique (CNRS), Grenoble (France). Inst. de Biologie Structurale (IBS); Inst. Laue-Langevin (ILL), Grenoble (France)
  3. Univ. of Oxford (United Kingdom)
  4. European Synchrotron Radiation Facility (ESRF), Grenoble (France)
  5. Univ. of California, San Francisco, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
  6. Univ. of Notre Dame, IN (United States). Notre Dame Radiation Lab. (NDRL)
Publication Date:
Research Org.:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); National Science Foundation (NSF)
OSTI Identifier:
1608636
Grant/Contract Number:  
AC02-76SF00515; FC02-04ER15533; AC02-05CH11231; ANR-15-CE18-0005-02; ANR-17-CE11-0018-01; FA-AAP-2013-65-101349; GM124149; GM124169; GM103393; GM082250; DBI‐1625906
Resource Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 117; Journal Issue: 8; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; X-ray radiation damage; serial crystallography; room temperature synchrotron data collection

Citation Formats

de la Mora, Eugenio, Coquelle, Nicolas, Bury, Charles S., Rosenthal, Martin, Holton, James M., Carmichael, Ian, Garman, Elspeth F., Burghammer, Manfred, Colletier, Jacques-Philippe, and Weik, Martin. Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures. United States: N. p., 2020. Web. doi:10.1073/pnas.1821522117.
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de la Mora, Eugenio, Coquelle, Nicolas, Bury, Charles S., Rosenthal, Martin, Holton, James M., Carmichael, Ian, Garman, Elspeth F., Burghammer, Manfred, Colletier, Jacques-Philippe, & Weik, Martin. Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures. United States. https://doi.org/10.1073/pnas.1821522117
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de la Mora, Eugenio, Coquelle, Nicolas, Bury, Charles S., Rosenthal, Martin, Holton, James M., Carmichael, Ian, Garman, Elspeth F., Burghammer, Manfred, Colletier, Jacques-Philippe, and Weik, Martin. Tue . "Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures". United States. https://doi.org/10.1073/pnas.1821522117. https://www.osti.gov/servlets/purl/1608636.
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@article{osti_1608636,
title = {Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures},
author = {de la Mora, Eugenio and Coquelle, Nicolas and Bury, Charles S. and Rosenthal, Martin and Holton, James M. and Carmichael, Ian and Garman, Elspeth F. and Burghammer, Manfred and Colletier, Jacques-Philippe and Weik, Martin},
abstractNote = {Radiation damage limits the accuracy of macromolecular structures in X-ray crystallography. Cryogenic (cryo-) cooling reduces the global radiation damage rate and, therefore, became the method of choice over the past decades. The recent advent of serial crystallography, which spreads the absorbed energy over many crystals, thereby reducing damage, has rendered room temperature (RT) data collection more practical and also extendable to microcrystals, both enabling and requiring the study of specific and global radiation damage at RT. Here, we performed sequential serial raster-scanning crystallography using a microfocused synchrotron beam that allowed for the collection of two series of 40 and 90 full datasets at 2- and 1.9-Å resolution at a dose rate of 40.3 MGy/s on hen egg white lysozyme (HEWL) crystals at RT and cryotemperature, respectively. The diffraction intensity halved its initial value at average doses (D1/2) of 0.57 and 15.3 MGy at RT and 100 K, respectively. Specific radiation damage at RT was observed at disulfide bonds but not at acidic residues, increasing and then apparently reversing, a peculiar behavior that can be modeled by accounting for differential diffraction intensity decay due to the nonuniform illumination by the X-ray beam. Specific damage to disulfide bonds is evident early on at RT and proceeds at a fivefold higher rate than global damage. The decay modeling suggests it is advisable not to exceed a dose of 0.38 MGy per dataset in static and time-resolved synchrotron crystallography experiments at RT. This rough yardstick might change for proteins other than HEWL and at resolutions other than 2 Å.},
doi = {10.1073/pnas.1821522117},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 8,
volume = 117,
place = {United States},
year = {Tue Feb 11 00:00:00 EST 2020},
month = {Tue Feb 11 00:00:00 EST 2020}
}
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https://doi.org/10.1073/pnas.1821522117
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Works referenced in this record:

CrystFEL : a software suite for snapshot serial crystallography
journal, March 2012

  • White, Thomas A.; Kirian, Richard A.; Martin, Andrew V.
  • Journal of Applied Crystallography, Vol. 45, Issue 2
  • DOI: 10.1107/S0021889812002312

Lifetimes and spatio-temporal response of protein crystals in intense X-ray microbeams
journal, October 2017

Conformational variation of proteins at room temperature is not dominated by radiation damage
journal, January 2017

  • Russi, Silvia; González, Ana; Kenner, Lillian R.
  • Journal of Synchrotron Radiation, Vol. 24, Issue 1
  • DOI: 10.1107/S1600577516017343

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
journal, October 2018

  • Schulz, Eike C.; Mehrabi, Pedram; Müller-Werkmeister, Henrike M.
  • Nature Methods, Vol. 15, Issue 11
  • DOI: 10.1038/s41592-018-0180-2

Mounting of crystals for macromolecular crystallography in a free-standing thin film
journal, October 1990

Serial crystallography on in vivo grown microcrystals using synchrotron radiation
journal, February 2014

Room-temperature macromolecular serial crystallography using synchrotron radiation
journal, May 2014

Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography
journal, July 2019

High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
journal, May 2012

Exploiting fast detectors to enter a new dimension in room-temperature crystallography
journal, April 2014

  • Owen, Robin L.; Paterson, Neil; Axford, Danny
  • Acta Crystallographica Section D Biological Crystallography, Vol. 70, Issue 5
  • DOI: 10.1107/S1399004714005379

Liquid application method for time-resolved analyses by serial synchrotron crystallography
journal, September 2019

Effective scavenging at cryotemperatures: further increasing the dose tolerance of protein crystals
journal, April 2011

  • De la Mora, Eugenio; Carmichael, Ian; Garman, Elspeth F.
  • Journal of Synchrotron Radiation, Vol. 18, Issue 3
  • DOI: 10.1107/S0909049511007163

Towards an understanding of radiation damage in cryocooled macromolecular crystals
journal, April 2005

Femtosecond X-ray protein nanocrystallography
journal, February 2011

  • Chapman, Henry N.; Fromme, Petra; Barty, Anton
  • Nature, Vol. 470, Issue 7332, p. 73-77
  • DOI: 10.1038/nature09750

The oscillation method for crystals with very large unit cells
journal, November 1979

  • Winkler, F. K.; Schutt, C. E.; Harrison, S. C.
  • Acta Crystallographica Section A, Vol. 35, Issue 6
  • DOI: 10.1107/S0567739479002035

Evidence for β Scission in the Oxidation of Amino Acids
journal, May 2002

  • Wisniowski, Pawel; Carmichael, Ian; Fessenden, Richard W.
  • The Journal of Physical Chemistry A, Vol. 106, Issue 18
  • DOI: 10.1021/jp0139383

Radiation damage in protein crystals is reduced with a micron-sized X-ray beam
journal, March 2011

  • Sanishvili, R.; Yoder, D. W.; Pothineni, S. B.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 15
  • DOI: 10.1073/pnas.1017701108

Outrunning free radicals in room-temperature macromolecular crystallography
journal, June 2012

  • Owen, Robin L.; Axford, Danny; Nettleship, Joanne E.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 7
  • DOI: 10.1107/S0907444912012553

Mix-and-diffuse serial synchrotron crystallography
journal, October 2017

Radiation Damage in Macromolecular Crystallography
book, January 2017

Primary radiation damage of protein crystals by an intense synchrotron X-ray beam
journal, September 2000

Glass transition in thaumatin crystals revealed through temperature-dependent radiation-sensitivity measurements
journal, September 2010

  • Warkentin, Matthew; Thorne, Robert E.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 10
  • DOI: 10.1107/S0907444910035523

Potential for biomolecular imaging with femtosecond X-ray pulses
journal, August 2000

  • Neutze, Richard; Wouts, Remco; van der Spoel, David
  • Nature, Vol. 406, Issue 6797
  • DOI: 10.1038/35021099

Autoindexing diffraction images with iMosflm
journal, June 2013

  • Powell, Harold R.; Johnson, Owen; Leslie, Andrew G. W.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7
  • DOI: 10.1107/S0907444912048524

XDS
journal, January 2010

  • Kabsch, Wolfgang
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
  • DOI: 10.1107/S0907444909047337

Indexing in single-crystal diffractometry with an obstinate list of reflections
journal, April 1992

Specific chemical and structural damage to proteins produced by synchrotron radiation
journal, January 2000

  • Weik, M.; Ravelli, R. B. G.; Kryger, G.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 2
  • DOI: 10.1073/pnas.97.2.623

Cryo-protection of protein crystals against radiation damage in electron and X-ray diffraction
journal, July 1990

  • Henderson, Richard
  • Proceedings of the Royal Society of London. Series B: Biological Sciences, Vol. 241, Issue 1300, p. 6-8
  • DOI: 10.1098/rspb.1990.0057

Estimate your dose: RADDOSE-3D: RADDOSE-3D
journal, November 2017

  • Bury, Charles S.; Brooks-Bartlett, Jonathan C.; Walsh, Steven P.
  • Protein Science, Vol. 27, Issue 1
  • DOI: 10.1002/pro.3302

Protein crystal perfection and the nature of radiation damage
journal, July 1988

Photoreduction of the active site of the metalloprotein putidaredoxin by synchrotron radiation
journal, August 2007

  • Corbett, Mary C.; Latimer, Matthew J.; Poulos, Thomas L.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 9
  • DOI: 10.1107/S0907444907035160

Low-dose fixed-target serial synchrotron crystallography
journal, March 2017

  • Owen, Robin L.; Axford, Danny; Sherrell, Darren A.
  • Acta Crystallographica Section D Structural Biology, Vol. 73, Issue 4, p. 373-378
  • DOI: 10.1107/S2059798317002996

Observation of Decreased Radiation Damage at Higher Dose Rates in Room Temperature Protein Crystallography
journal, December 2007

  • Southworth-Davies, Robert J.; Medina, Melissa A.; Carmichael, Ian
  • Structure, Vol. 15, Issue 12
  • DOI: 10.1016/j.str.2007.10.013

Room-temperature serial crystallography at synchrotron X-ray sources using slowly flowing free-standing high-viscosity microstreams
journal, January 2015

  • Botha, Sabine; Nass, Karol; Barends, Thomas R. M.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 2
  • DOI: 10.1107/S1399004714026327

Radiation damage in protein crystallography
journal, September 1976

EIGER detector: application in macromolecular crystallography
journal, August 2016

  • Casanas, Arnau; Warshamanage, Rangana; Finke, Aaron D.
  • Acta Crystallographica Section D Structural Biology, Vol. 72, Issue 9
  • DOI: 10.1107/S2059798316012304

RADDOSE-3D : time- and space-resolved modelling of dose in macromolecular crystallography
journal, June 2013

  • Zeldin, Oliver B.; Gerstel, Markus; Garman, Elspeth F.
  • Journal of Applied Crystallography, Vol. 46, Issue 4
  • DOI: 10.1107/S0021889813011461

Structural Basis of X-ray-Induced Transient Photobleaching in a Photoactivatable Green Fluorescent Protein
journal, December 2009

  • Adam, Virgile; Carpentier, Philippe; Violot, Sebastien
  • Journal of the American Chemical Society, Vol. 131, Issue 50
  • DOI: 10.1021/ja907296v

Insights into the mechanism of X-ray-induced disulfide-bond cleavage in lysozyme crystals based on EPR, optical absorption and X-ray diffraction studies
journal, November 2013

  • Sutton, Kristin A.; Black, Paul J.; Mercer, Kermit R.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 12
  • DOI: 10.1107/S0907444913022117

Acoustic methods for high-throughput protein crystal mounting at next-generation macromolecular crystallographic beamlines
journal, August 2013

  • Roessler, Christian G.; Kuczewski, Anthony; Stearns, Richard
  • Journal of Synchrotron Radiation, Vol. 20, Issue 5
  • DOI: 10.1107/S0909049513020372

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

The ‘fingerprint’ that X-rays can leave on structures
journal, March 2000

X-ray damage to the Mn4Ca complex in single crystals of photosystem II: A case study for metalloprotein crystallography
journal, August 2005

  • Yano, J.; Kern, J.; Irrgang, K. -D.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 34
  • DOI: 10.1073/pnas.0505207102

Time-resolved crystallography reveals allosteric communication aligned with molecular breathing
journal, September 2019

Cryocooling and radiation damage in macromolecular crystallography
journal, December 2005

  • Garman, Elspeth F.; Owen, Robin Leslie
  • Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 1
  • DOI: 10.1107/S0907444905034207

An assessment of the resolution limitation due to radiation-damage in X-ray diffraction microscopy
journal, March 2009

  • Howells, M. R.; Beetz, T.; Chapman, H. N.
  • Journal of Electron Spectroscopy and Related Phenomena, Vol. 170, Issue 1-3
  • DOI: 10.1016/j.elspec.2008.10.008

How does Radiation Damage in Protein Crystals Depend on X-Ray Dose?
journal, January 2003

Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams
journal, April 2015

  • Coquelle, Nicolas; Brewster, Aaron S.; Kapp, Ulrike
  • Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 5
  • DOI: 10.1107/S1399004715004514

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering
journal, May 2016

  • Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan
  • Journal of Applied Crystallography, Vol. 49, Issue 3
  • DOI: 10.1107/S1600576716006348

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
journal, March 2015

Structural changes in a cryo-cooled protein crystal owing to radiation damage
journal, March 2000

  • Burmeister, Wilhelm Pascal
  • Acta Crystallographica Section D Biological Crystallography, Vol. 56, Issue 3
  • DOI: 10.1107/S0907444999016261

Resolution and dose dependence of radiation damage in biomolecular systems
journal, September 2019

Version 1.2 of the Crystallography and NMR system
journal, October 2007

Cryocrystallography of biological macromolecules: a generally applicable method
journal, February 1988

Translational diffusion of hydration water correlates with functional motions in folded and intrinsically disordered proteins
journal, March 2015

  • Schirò, Giorgio; Fichou, Yann; Gallat, Francois-Xavier
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms7490

Pink-beam serial crystallography
journal, November 2017

Will reduced radiation damage occur with very small crystals?
journal, April 2005

A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering
journal, May 2015

  • Roedig, P.; Vartiainen, I.; Duman, R.
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep10451

Experimental determination of the radiation dose limit for cryocooled protein crystals
journal, March 2006

  • Owen, R. L.; Rudino-Pinera, E.; Garman, E. F.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 13
  • DOI: 10.1073/pnas.0600973103

Crystallography on a chip – without the chip: sheet-on-sheet sandwich
journal, October 2018

  • Doak, R. Bruce; Nass Kovacs, Gabriela; Gorel, Alexander
  • Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 10
  • DOI: 10.1107/S2059798318011634

Generation of Size-Controlled, Submicrometer Protein Crystals
journal, May 2005

  • Falkner, Joshua C.; Al-Somali, Ali M.; Jamison, Jennifer A.
  • Chemistry of Materials, Vol. 17, Issue 10
  • DOI: 10.1021/cm047924w

A microfluidic flow-focusing device for low sample consumption serial synchrotron crystallography experiments in liquid flow
journal, February 2019

  • Monteiro, Diana C. F.; Vakili, Mohammad; Harich, Jessica
  • Journal of Synchrotron Radiation, Vol. 26, Issue 2
  • DOI: 10.1107/S1600577519000304

Accessing protein conformational ensembles using room-temperature X-ray crystallography
journal, September 2011

  • Fraser, J. S.; van den Bedem, H.; Samelson, A. J.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 39
  • DOI: 10.1073/pnas.1111325108

Improved Estimation of Structure-Factor Difference Amplitudesfrom Poorly Accurate Data
journal, September 1997

  • Ursby, T.; Bourgeois, D.
  • Acta Crystallographica Section A Foundations of Crystallography, Vol. 53, Issue 5
  • DOI: 10.1107/S0108767397004522

A multicrystal diffraction data-collection approach for studying structural dynamics with millisecond temporal resolution
journal, October 2016

Raman-Assisted Crystallography Suggests a Mechanism of X-Ray-Induced Disulfide Radical Formation and Reparation
journal, November 2010

Radiation Damage in Macromolecular Crystallography
book, January 2017

Protein crystal perfection and the nature of radiation damage
journal, July 1988

Radiation damage in protein crystallography
journal, September 1976

Observation of Decreased Radiation Damage at Higher Dose Rates in Room Temperature Protein Crystallography
journal, December 2007

  • Southworth-Davies, Robert J.; Medina, Melissa A.; Carmichael, Ian
  • Structure, Vol. 15, Issue 12
  • DOI: 10.1016/j.str.2007.10.013

Raman-Assisted Crystallography Suggests a Mechanism of X-Ray-Induced Disulfide Radical Formation and Reparation
journal, November 2010

Structural Basis of X-ray-Induced Transient Photobleaching in a Photoactivatable Green Fluorescent Protein
journal, December 2009

  • Adam, Virgile; Carpentier, Philippe; Violot, Sebastien
  • Journal of the American Chemical Society, Vol. 131, Issue 50
  • DOI: 10.1021/ja907296v

Femtosecond X-ray protein nanocrystallography
journal, February 2011

  • Chapman, Henry N.; Fromme, Petra; Barty, Anton
  • Nature, Vol. 470, Issue 7332, p. 73-77
  • DOI: 10.1038/nature09750

Version 1.2 of the Crystallography and NMR system
journal, October 2007

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
journal, October 2018

  • Schulz, Eike C.; Mehrabi, Pedram; Müller-Werkmeister, Henrike M.
  • Nature Methods, Vol. 15, Issue 11
  • DOI: 10.1038/s41592-018-0180-2

A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering
journal, May 2015

  • Roedig, P.; Vartiainen, I.; Duman, R.
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep10451

Some simple, highly reactive, inorganic chlorine derivatives in aqueous solution. Their formation using pulses of radiation and their role in the mechanism of the Fricke dosimeter
journal, January 1973

  • Jayson, G. G.; Parsons, B. J.; Swallow, A. J.
  • Journal of the Chemical Society, Faraday Transactions 1: Physical Chemistry in Condensed Phases, Vol. 69, Issue 0
  • DOI: 10.1039/f19736901597

X-ray damage to the Mn4Ca complex in single crystals of photosystem II: A case study for metalloprotein crystallography
journal, August 2005

  • Yano, J.; Kern, J.; Irrgang, K. -D.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 34
  • DOI: 10.1073/pnas.0505207102

Experimental determination of the radiation dose limit for cryocooled protein crystals
journal, March 2006

  • Owen, R. L.; Rudino-Pinera, E.; Garman, E. F.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 13
  • DOI: 10.1073/pnas.0600973103

Radiation damage in protein crystals is reduced with a micron-sized X-ray beam
journal, March 2011

  • Sanishvili, R.; Yoder, D. W.; Pothineni, S. B.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 15
  • DOI: 10.1073/pnas.1017701108

Specific chemical and structural damage to proteins produced by synchrotron radiation
journal, January 2000

  • Weik, M.; Ravelli, R. B. G.; Kryger, G.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 2
  • DOI: 10.1073/pnas.97.2.623

Resolution and dose dependence of radiation damage in biomolecular systems
journal, July 2019

  • Atakisi, Hakan; Conger, Lauren; Moreau, David
  • Acta Crystallographica Section A Foundations and Advances, Vol. 75, Issue a1
  • DOI: 10.1107/s0108767319096521

Sequential radiation damage in protein crystallography
journal, July 1988

  • Sygusch, J.; Allaire, M.
  • Acta Crystallographica Section A Foundations of Crystallography, Vol. 44, Issue 4
  • DOI: 10.1107/s0108767388001394

Use of a `caged' analogue to study the traffic of choline within acetylcholinesterase by kinetic crystallography
journal, October 2007

  • Colletier, Jacques-Philippe; Royant, Antoine; Specht, Alexandre
  • Acta Crystallographica Section D Biological Crystallography, Vol. 63, Issue 11
  • DOI: 10.1107/s0907444907044472

Global radiation damage at 300 and 260 K with dose rates approaching 1 MGy s −1
journal, January 2012

  • Warkentin, Matthew; Badeau, Ryan; Hopkins, Jesse B.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 2
  • DOI: 10.1107/s0907444911052085

XANES measurements of the rate of radiation damage to selenomethionine side chains
journal, December 2006

Temperature-dependent radiation sensitivity and order of 70S ribosome crystals
journal, October 2014

  • Warkentin, Matthew; Hopkins, Jesse B.; Haber, Jonah B.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 70, Issue 11
  • DOI: 10.1107/s1399004714017672

RIDL : a tool to investigate radiation-induced density loss
journal, May 2018

  • Bury, Charles Simon; Garman, Elspeth Frances
  • Journal of Applied Crystallography, Vol. 51, Issue 3
  • DOI: 10.1107/s1600576718005241

Dose-resolved serial synchrotron and XFEL structures of radiation-sensitive metalloproteins
journal, May 2019

Proton uptake mechanism in bacteriorhodopsin captured by serial synchrotron crystallography
journal, July 2019

Time-resolved crystallography reveals allosteric communication aligned with molecular breathing
journal, September 2019

Room-Temperature Macromolecular Crystallography Using a Micro-Patterned Silicon Chip with Minimal Background Scattering
text, January 2016

  • Roedig, Philip; Duman, Ramona; Sanchez-Weatherby, Juan
  • Deutsches Elektronen-Synchrotron, DESY, Hamburg
  • DOI: 10.3204/pubdb-2016-02690
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