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Title: Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.

Abstract

Nannochloropsis is a genus of fast-growing microalgae that are regularly used for biotechnology applications. Nannochloropsis species have a high triacylglycerol content and their polar lipids are rich in the omega-3 long-chain polyunsaturated fatty acid, eicosapentaenoic acid. Placed in the heterokont lineage, the Nannochloropsis genus has a complex evolutionary history. Genome sequences are available for several species, and a number of transcriptomic datasets have been produced, making this genus a facile model for comparative genomics. There is a growing interest in Nannochloropsis species as models for the study of microalga lipid metabolism and as a chassis for synthetic biology. Recently, techniques for gene stacking, and targeted gene disruption and repression in the Nannochloropsis genus have been developed. These tools enable gene-specific, mechanistic studies and have already allowed the engineering of improved Nannochloropsis strains with superior growth, or greater bioproduction.

Authors:
 [1];  [1]; ORCiD logo [1]
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  1. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1608420
Grant/Contract Number:  
FG02-91ER20021
Resource Type:
Accepted Manuscript
Journal Name:
Plant Cell Reports
Additional Journal Information:
Journal Volume: 37; Journal Issue: 10; Journal ID: ISSN 0721-7714
Publisher:
Springer Nature
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Poliner, Eric, Farré, Eva M., and Benning, Christoph. Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.. United States: N. p., 2018. Web. doi:10.1007/s00299-018-2270-0.
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Poliner, Eric, Farré, Eva M., & Benning, Christoph. Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.. United States. https://doi.org/10.1007/s00299-018-2270-0
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Poliner, Eric, Farré, Eva M., and Benning, Christoph. Tue . "Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.". United States. https://doi.org/10.1007/s00299-018-2270-0. https://www.osti.gov/servlets/purl/1608420.
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@article{osti_1608420,
title = {Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.},
author = {Poliner, Eric and Farré, Eva M. and Benning, Christoph},
abstractNote = {Nannochloropsis is a genus of fast-growing microalgae that are regularly used for biotechnology applications. Nannochloropsis species have a high triacylglycerol content and their polar lipids are rich in the omega-3 long-chain polyunsaturated fatty acid, eicosapentaenoic acid. Placed in the heterokont lineage, the Nannochloropsis genus has a complex evolutionary history. Genome sequences are available for several species, and a number of transcriptomic datasets have been produced, making this genus a facile model for comparative genomics. There is a growing interest in Nannochloropsis species as models for the study of microalga lipid metabolism and as a chassis for synthetic biology. Recently, techniques for gene stacking, and targeted gene disruption and repression in the Nannochloropsis genus have been developed. These tools enable gene-specific, mechanistic studies and have already allowed the engineering of improved Nannochloropsis strains with superior growth, or greater bioproduction.},
doi = {10.1007/s00299-018-2270-0},
journal = {Plant Cell Reports},
number = 10,
volume = 37,
place = {United States},
year = {Tue Mar 06 00:00:00 EST 2018},
month = {Tue Mar 06 00:00:00 EST 2018}
}
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  • DOI: 10.1104/pp.17.00493

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
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