3D-Printed Hydrogel Composites for Predictive Temporal (4D) Cellular Organizations and Patterned Biogenic Mineralization
Abstract
Abstract Materials chemistries for hydrogel scaffolds that are capable of programming temporal (4D) attributes of cellular decision‐making in supported 3D microcultures are described. The scaffolds are fabricated using direct‐ink writing (DIW)—a 3D‐printing technique using extrusion to pattern scaffolds at biologically relevant diameters (≤ 100 µm). Herein, DIW is exploited to variously incorporate a rheological nanoclay, Laponite XLG (LAP), into 2‐hydroxyethyl methacrylate (HEMA)‐based hydrogels—printing the LAP–HEMA (LH) composites as functional modifiers within otherwise unmodified 2D and 3D HEMA microstructures. The nanoclay‐modified domains, when tested as thin films, require no activating (e.g., protein) treatments to promote robust growth compliances that direct the spatial attachment of fibroblast (3T3) and preosteoblast (E1) cells, fostering for the latter a capacity to direct long‐term osteodifferentiation. Cell‐to‐gel interfacial morphologies and cellular motility are analyzed with spatial light interference microscopy (SLIM). Through combination of HEMA and LH gels, high‐resolution DIW of a nanocomposite ink (UniH) that translates organizationally dynamic attributes seen with 2D gels into dentition‐mimetic 3D scaffolds is demonstrated. These analyses confirm that the underlying materials chemistry and geometry of hydrogel nanocomposites are capable of directing cellular attachment and temporal development within 3D microcultures—a useful material system for the 4D patterning of hydrogel scaffolds.
- Authors:
-
[1];
[2];
[3];
[1];
[5]
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Beckman Institute, Urbana, IL (United States)
- Univ. of Illinois at Urbana-Champaign, IL (United States). Frederick Seitz Materials Research Laboratory
- Univ. of Illinois at Urbana-Champaign, IL (United States). Frederick Seitz Materials Research Laboratory and Department of Materials Science and Engineering
- Univ. of Illinois at Urbana-Champaign, IL (United States). Frederick Seitz Materials Research Laboratory; KTH Royal Inst. of Technology, Stockholm (Sweden)
- Publication Date:
- Research Org.:
- Univ. of Illinois at Urbana-Champaign, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Materials Sciences & Engineering Division; USDOE
- OSTI Identifier:
- 1607430
- Alternate Identifier(s):
- OSTI ID: 1488354
- Grant/Contract Number:
- FG02-07ER46471; DE‐FG02‐07ER46471
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Advanced Healthcare Materials
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2192-2640
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 3D printing; biomineralization; cellular biocompliance; direct ink write 4D printing; hydrogel nanocomposites
Citation Formats
McCracken, Joselle M., Rauzan, Brittany M., Kjellman, Jacob C. E., Kandel, Mikhail E., Liu, Yu Hao, Badea, Adina, Miller, Lou Ann, Rogers, Simon A., Popescu, Gabriel, and Nuzzo, Ralph G. 3D-Printed Hydrogel Composites for Predictive Temporal (4D) Cellular Organizations and Patterned Biogenic Mineralization. United States: N. p., 2018.
Web. doi:10.1002/adhm.201800788.
McCracken, Joselle M., Rauzan, Brittany M., Kjellman, Jacob C. E., Kandel, Mikhail E., Liu, Yu Hao, Badea, Adina, Miller, Lou Ann, Rogers, Simon A., Popescu, Gabriel, & Nuzzo, Ralph G. 3D-Printed Hydrogel Composites for Predictive Temporal (4D) Cellular Organizations and Patterned Biogenic Mineralization. United States. https://doi.org/10.1002/adhm.201800788
McCracken, Joselle M., Rauzan, Brittany M., Kjellman, Jacob C. E., Kandel, Mikhail E., Liu, Yu Hao, Badea, Adina, Miller, Lou Ann, Rogers, Simon A., Popescu, Gabriel, and Nuzzo, Ralph G. Thu .
"3D-Printed Hydrogel Composites for Predictive Temporal (4D) Cellular Organizations and Patterned Biogenic Mineralization". United States. https://doi.org/10.1002/adhm.201800788. https://www.osti.gov/servlets/purl/1607430.
@article{osti_1607430,
title = {3D-Printed Hydrogel Composites for Predictive Temporal (4D) Cellular Organizations and Patterned Biogenic Mineralization},
author = {McCracken, Joselle M. and Rauzan, Brittany M. and Kjellman, Jacob C. E. and Kandel, Mikhail E. and Liu, Yu Hao and Badea, Adina and Miller, Lou Ann and Rogers, Simon A. and Popescu, Gabriel and Nuzzo, Ralph G.},
abstractNote = {Abstract Materials chemistries for hydrogel scaffolds that are capable of programming temporal (4D) attributes of cellular decision‐making in supported 3D microcultures are described. The scaffolds are fabricated using direct‐ink writing (DIW)—a 3D‐printing technique using extrusion to pattern scaffolds at biologically relevant diameters (≤ 100 µm). Herein, DIW is exploited to variously incorporate a rheological nanoclay, Laponite XLG (LAP), into 2‐hydroxyethyl methacrylate (HEMA)‐based hydrogels—printing the LAP–HEMA (LH) composites as functional modifiers within otherwise unmodified 2D and 3D HEMA microstructures. The nanoclay‐modified domains, when tested as thin films, require no activating (e.g., protein) treatments to promote robust growth compliances that direct the spatial attachment of fibroblast (3T3) and preosteoblast (E1) cells, fostering for the latter a capacity to direct long‐term osteodifferentiation. Cell‐to‐gel interfacial morphologies and cellular motility are analyzed with spatial light interference microscopy (SLIM). Through combination of HEMA and LH gels, high‐resolution DIW of a nanocomposite ink (UniH) that translates organizationally dynamic attributes seen with 2D gels into dentition‐mimetic 3D scaffolds is demonstrated. These analyses confirm that the underlying materials chemistry and geometry of hydrogel nanocomposites are capable of directing cellular attachment and temporal development within 3D microcultures—a useful material system for the 4D patterning of hydrogel scaffolds.},
doi = {10.1002/adhm.201800788},
journal = {Advanced Healthcare Materials},
number = 1,
volume = 8,
place = {United States},
year = {Thu Nov 22 00:00:00 EST 2018},
month = {Thu Nov 22 00:00:00 EST 2018}
}
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