Single-particle structure determination by X-ray free-electron lasers: Possibilities and challenges
Abstract
Single-particle structure recovery without crystals or radiation damage is a revolutionary possibility offered by X-ray free-electron lasers, but it involves formidable experimental and data-analytical challenges. Many of these difficulties were encountered during the development of cryogenic electron microscopy of biological systems. Electron microscopy of biological entities has now reached a spatial resolution of about 0.3 nm, with a rapidly emerging capability to map discrete and continuous conformational changes and the energy landscapes of biomolecular machines. Nonetheless, single-particle imaging by X-ray freeelectron lasers remains important for a range of applications, including the study of large “electron-opaque” objects and time-resolved examination of key biological processes at physiological temperatures. After summarizing the state of the art in the study of structure and conformations by cryogenic electron microscopy, we identify the primary opportunities and challenges facing X-ray-based single-particle approaches, and possible means for circumventing them.
- Authors:
-
- Univ. of Wisconsin, Milwaukee, WI (United States)
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Milwaukee, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)
- OSTI Identifier:
- 1598194
- Alternate Identifier(s):
- OSTI ID: 1421053
- Grant/Contract Number:
- FG02-09ER16114; SC0002164
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Structural Dynamics
- Additional Journal Information:
- Journal Volume: 2; Journal Issue: 4; Journal ID: ISSN 2329-7778
- Publisher:
- American Crystallographic Association/AIP
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS
Citation Formats
Hosseinizadeh, A., Dashti, A., Schwander, P., Fung, R., and Ourmazd, A. Single-particle structure determination by X-ray free-electron lasers: Possibilities and challenges. United States: N. p., 2015.
Web. doi:10.1063/1.4919740.
Hosseinizadeh, A., Dashti, A., Schwander, P., Fung, R., & Ourmazd, A. Single-particle structure determination by X-ray free-electron lasers: Possibilities and challenges. United States. https://doi.org/10.1063/1.4919740
Hosseinizadeh, A., Dashti, A., Schwander, P., Fung, R., and Ourmazd, A. Thu .
"Single-particle structure determination by X-ray free-electron lasers: Possibilities and challenges". United States. https://doi.org/10.1063/1.4919740. https://www.osti.gov/servlets/purl/1598194.
@article{osti_1598194,
title = {Single-particle structure determination by X-ray free-electron lasers: Possibilities and challenges},
author = {Hosseinizadeh, A. and Dashti, A. and Schwander, P. and Fung, R. and Ourmazd, A.},
abstractNote = {Single-particle structure recovery without crystals or radiation damage is a revolutionary possibility offered by X-ray free-electron lasers, but it involves formidable experimental and data-analytical challenges. Many of these difficulties were encountered during the development of cryogenic electron microscopy of biological systems. Electron microscopy of biological entities has now reached a spatial resolution of about 0.3 nm, with a rapidly emerging capability to map discrete and continuous conformational changes and the energy landscapes of biomolecular machines. Nonetheless, single-particle imaging by X-ray freeelectron lasers remains important for a range of applications, including the study of large “electron-opaque” objects and time-resolved examination of key biological processes at physiological temperatures. After summarizing the state of the art in the study of structure and conformations by cryogenic electron microscopy, we identify the primary opportunities and challenges facing X-ray-based single-particle approaches, and possible means for circumventing them.},
doi = {10.1063/1.4919740},
journal = {Structural Dynamics},
number = 4,
volume = 2,
place = {United States},
year = {Thu Apr 30 00:00:00 EDT 2015},
month = {Thu Apr 30 00:00:00 EDT 2015}
}
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