In Brachypodium a complex signaling is actuated to protect cells from proteotoxic stress and facilitate seed filling
Abstract
A conserved UPR machinery is necessary for Brachypodium ER stress resistance and grain filling. Human and livestock diets depend on the accumulation of cereal storage proteins and carbohydrates, including mixed-linkage glucan (MLG), in the endosperm during seed development. Storage proteins and proteins responsible for the production of carbohydrates are synthesized in the endoplasmic reticulum (ER). Unfavorable conditions during growth that hamper the ER biosynthetic capacity, such as heat, can cause a potentially lethal condition known as ER stress, which activates the unfolded protein response (UPR), a signaling response designed to mitigate ER stress. The UPR relies primarily on a conserved ER-associated kinase and ribonuclease, IRE1, which splices the mRNA of a transcription factor (TF), such as bZIP60 in plants, to produce an active TF that controls the expression of ER resident chaperones. Here, we investigated activation of the UPR in Brachypodium, as a model to study the UPR in seeds of a monocotyledon species, as well as the consequences of heat stress on MLG deposition in seeds. We identified a Brachypodium bZIP60 orthologue and determined a positive correlation between bZIP60 splicing and ER stress induced by chemicals and heat. Each stress condition led to transcriptional modulation of several BiP genes,more »
- Publication Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; National Institutes of Health (NHI)
- OSTI Identifier:
- 1596268
- Grant/Contract Number:
- FG02-91ER20021; FC02-07ER64494; GM101038
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Planta
- Additional Journal Information:
- Journal Volume: 246; Journal Issue: 1; Journal ID: ISSN 0032-0935
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Kim, Sang-Jin, Zemelis-Durfee, Starla, Wilkerson, Curtis, and Brandizzi, Federica. In Brachypodium a complex signaling is actuated to protect cells from proteotoxic stress and facilitate seed filling. United States: N. p., 2017.
Web. doi:10.1007/s00425-017-2687-7.
Kim, Sang-Jin, Zemelis-Durfee, Starla, Wilkerson, Curtis, & Brandizzi, Federica. In Brachypodium a complex signaling is actuated to protect cells from proteotoxic stress and facilitate seed filling. United States. https://doi.org/10.1007/s00425-017-2687-7
Kim, Sang-Jin, Zemelis-Durfee, Starla, Wilkerson, Curtis, and Brandizzi, Federica. Fri .
"In Brachypodium a complex signaling is actuated to protect cells from proteotoxic stress and facilitate seed filling". United States. https://doi.org/10.1007/s00425-017-2687-7. https://www.osti.gov/servlets/purl/1596268.
@article{osti_1596268,
title = {In Brachypodium a complex signaling is actuated to protect cells from proteotoxic stress and facilitate seed filling},
author = {Kim, Sang-Jin and Zemelis-Durfee, Starla and Wilkerson, Curtis and Brandizzi, Federica},
abstractNote = {A conserved UPR machinery is necessary for Brachypodium ER stress resistance and grain filling. Human and livestock diets depend on the accumulation of cereal storage proteins and carbohydrates, including mixed-linkage glucan (MLG), in the endosperm during seed development. Storage proteins and proteins responsible for the production of carbohydrates are synthesized in the endoplasmic reticulum (ER). Unfavorable conditions during growth that hamper the ER biosynthetic capacity, such as heat, can cause a potentially lethal condition known as ER stress, which activates the unfolded protein response (UPR), a signaling response designed to mitigate ER stress. The UPR relies primarily on a conserved ER-associated kinase and ribonuclease, IRE1, which splices the mRNA of a transcription factor (TF), such as bZIP60 in plants, to produce an active TF that controls the expression of ER resident chaperones. Here, we investigated activation of the UPR in Brachypodium, as a model to study the UPR in seeds of a monocotyledon species, as well as the consequences of heat stress on MLG deposition in seeds. We identified a Brachypodium bZIP60 orthologue and determined a positive correlation between bZIP60 splicing and ER stress induced by chemicals and heat. Each stress condition led to transcriptional modulation of several BiP genes, supporting the existence of condition-specific BiP regulation. Finally, we found that the UPR is elevated at the early stage of seed development and that MLG production is negatively affected by heat stress via modulation of MLG synthase accumulation. We present that successful accomplishment of seed filling is strongly correlated with the ability of the plant to sustain ER stress via the UPR.},
doi = {10.1007/s00425-017-2687-7},
journal = {Planta},
number = 1,
volume = 246,
place = {United States},
year = {Fri Mar 31 00:00:00 EDT 2017},
month = {Fri Mar 31 00:00:00 EDT 2017}
}
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Works referencing / citing this record:
In the grass species Brachypodium distachyon , the production of mixed‐linkage (1,3;1,4)‐β‐glucan ( MLG ) occurs in the Golgi apparatus
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