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Title: Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina

Abstract

The biological production of methane is vital to the global carbon cycle and accounts for ca. 74% of total methane emissions. The organisms that facilitate this process, methanogenic archaea, belong to a large and phylogenetically diverse group that thrives in a wide range of anaerobic environments. Two main subgroups exist within methanogenic archaea: those with and those without cytochromes. Although a variety of metabolisms exist within this group, the reduction of growth substrates to methane using electrons from molecular hydrogen is, in a phylogenetic sense, the most widespread methanogenic pathway. Methanogens without cytochromes typically generate methane by the reduction of CO2 with electrons derived from H2, formate, or secondary alcohols, generating a transmembrane ion gradient for ATP production via an Na+-translocating methyltransferase (Mtr). These organisms also conserve energy with a novel flavin-based electron bifurcation mechanism, wherein the endergonic reduction of ferredoxin is facilitated by the exergonic reduction of a disulfide terminal electron acceptor coupled to either H2 or formate oxidation. Methanogens that utilize cytochromes have a broader substrate range, and can convert acetate and methylated compounds to methane, in addition to the ability to reduce CO2. Cytochrome-containing methanogens are able to supplement the ion motive force generated by Mtr withmore » an H+-translocating electron transport system. In both groups, enzymes known as hydrogenases, which reversibly interconvert protons and electrons to molecular hydrogen, play a central role in the methanogenic process. In this review, we discuss recent insight into methanogen metabolism and energy conservation mechanisms with a particular focus on the genus Methanosarcina.« less

Authors:
 [1]; ORCiD logo [1]
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  1. Univ. of Illinois at Urbana-Champaign, IL (United States)
Publication Date:
Research Org.:
William W. Metcalf (University of Illinois at Urbana-Champaign
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
OSTI Identifier:
1595766
Grant/Contract Number:  
FG02-02ER15296
Resource Type:
Accepted Manuscript
Journal Name:
Microbiology and Molecular Biology Reviews
Additional Journal Information:
Journal Volume: 83; Journal Issue: 4; Journal ID: ISSN 1092-2172
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; archaea; energy conservation; hydrogenase; methanogenesis

Citation Formats

Mand, Thomas D., and Metcalf, William W. Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina. United States: N. p., 2019. Web. doi:10.1128/MMBR.00020-19.
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Mand, Thomas D., & Metcalf, William W. Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina. United States. https://doi.org/10.1128/MMBR.00020-19
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Mand, Thomas D., and Metcalf, William W. Wed . "Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina". United States. https://doi.org/10.1128/MMBR.00020-19. https://www.osti.gov/servlets/purl/1595766.
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@article{osti_1595766,
title = {Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina},
author = {Mand, Thomas D. and Metcalf, William W.},
abstractNote = {The biological production of methane is vital to the global carbon cycle and accounts for ca. 74% of total methane emissions. The organisms that facilitate this process, methanogenic archaea, belong to a large and phylogenetically diverse group that thrives in a wide range of anaerobic environments. Two main subgroups exist within methanogenic archaea: those with and those without cytochromes. Although a variety of metabolisms exist within this group, the reduction of growth substrates to methane using electrons from molecular hydrogen is, in a phylogenetic sense, the most widespread methanogenic pathway. Methanogens without cytochromes typically generate methane by the reduction of CO2 with electrons derived from H2, formate, or secondary alcohols, generating a transmembrane ion gradient for ATP production via an Na+-translocating methyltransferase (Mtr). These organisms also conserve energy with a novel flavin-based electron bifurcation mechanism, wherein the endergonic reduction of ferredoxin is facilitated by the exergonic reduction of a disulfide terminal electron acceptor coupled to either H2 or formate oxidation. Methanogens that utilize cytochromes have a broader substrate range, and can convert acetate and methylated compounds to methane, in addition to the ability to reduce CO2. Cytochrome-containing methanogens are able to supplement the ion motive force generated by Mtr with an H+-translocating electron transport system. In both groups, enzymes known as hydrogenases, which reversibly interconvert protons and electrons to molecular hydrogen, play a central role in the methanogenic process. In this review, we discuss recent insight into methanogen metabolism and energy conservation mechanisms with a particular focus on the genus Methanosarcina.},
doi = {10.1128/MMBR.00020-19},
journal = {Microbiology and Molecular Biology Reviews},
number = 4,
volume = 83,
place = {United States},
year = {Wed Sep 18 00:00:00 EDT 2019},
month = {Wed Sep 18 00:00:00 EDT 2019}
}
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https://doi.org/10.1128/MMBR.00020-19
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Methanocella paludicola gen. nov., sp. nov., a methane-producing archaeon, the first isolate of the lineage 'Rice Cluster I', and proposal of the new archaeal order Methanocellales ord. nov.
journal, April 2008

  • Sakai, S.; Imachi, H.; Hanada, S.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 58, Issue 4
  • DOI: 10.1099/ijs.0.65571-0

Methane production from coal by a single methanogen
journal, October 2016

Sodium dependence of growth and methane formation in Methanobacterium thermoautotrophicum
journal, January 1981

  • Perski, Hans-Joachim; Moll, Johanna; Thauer, Rudolf K.
  • Archives of Microbiology, Vol. 130, Issue 4
  • DOI: 10.1007/BF00425947

Hydrogenases from Methanogenic Archaea, Nickel, a Novel Cofactor, and H 2 Storage
journal, June 2010

Physiological role of the F 420 -non-reducing hydrogenase (Mvh) from Methanothermobacter marburgensis
journal, September 2003

  • Stojanowic, Alexander; Mander, Gerd J.; Duin, Evert C.
  • Archives of Microbiology, Vol. 180, Issue 3
  • DOI: 10.1007/s00203-003-0577-9

Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta H.
journal, February 1990

  • te Brömmelstroet, B. W.; Hensgens, C. M.; Keltjens, J. T.
  • Journal of Biological Chemistry, Vol. 265, Issue 4
  • DOI: 10.1016/S0021-9258(19)39907-7

Energy Conservation by the H2:Heterodisulfide Oxidoreductase from Methanosarcina mazei Gö1: Identification of Two Proton-Translocating Segments
journal, July 1999

Novel biochemistry of methanogenesis.
journal, June 1988

Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum.
journal, April 1982

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