Intrinsically disordered proteins access a range of hysteretic phase separation behaviors
Abstract
The phase separation behavior of intrinsically disordered proteins (IDPs) is thought of as analogous to that of polymers that undergo equilibrium lower or upper critical solution temperature (LCST and UCST, respectively) phase transition. This view, however, ignores possible nonequilibrium properties of protein assemblies. Here, by studying IDP polymers (IDPPs) composed of repeat motifs that encode LCST or UCST phase behavior, we discovered that IDPs can access a wide spectrum of nonequilibrium, hysteretic phase behaviors. Experimentally and through simulations, we show that hysteresis in IDPPs is tunable and that it emerges through increasingly stable interchain interactions in the insoluble phase. To explore the utility of hysteretic IDPPs, we engineer self-assembling nanostructures with tunable stability. These findings shine light on the rich phase separation behavior of IDPs and illustrate hysteresis as a design parameter to program nonequilibrium phase behavior in self-assembling materials.
- Authors:
-
[1];
[2];
[1];
[1];
[2];
[1]
- Duke Univ., Durham, NC (United States)
- North Carolina State Univ., Raleigh, NC (United States)
- Univ. of Basel (Switzerland). Swiss Nanoscience Inst.
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Institutes of Health (NIH); National Science Foundation (NSF)
- OSTI Identifier:
- 1580951
- Grant/Contract Number:
- AC02-05CH11231; R01 GM061232; MIRA R35GM127042; NSF-DMR-1729671
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Science Advances
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 10; Journal ID: ISSN 2375-2548
- Publisher:
- AAAS
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Garcia Quiroz, Felipe, Li, Nan K., Roberts, Stefan, Weber, Patrick, Dzuricky, Michael, Weitzhandler, Isaac, Yingling, Yaroslava G., and Chilkoti, Ashutosh. Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. United States: N. p., 2019.
Web. doi:10.1126/sciadv.aax5177.
Garcia Quiroz, Felipe, Li, Nan K., Roberts, Stefan, Weber, Patrick, Dzuricky, Michael, Weitzhandler, Isaac, Yingling, Yaroslava G., & Chilkoti, Ashutosh. Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. United States. https://doi.org/10.1126/sciadv.aax5177
Garcia Quiroz, Felipe, Li, Nan K., Roberts, Stefan, Weber, Patrick, Dzuricky, Michael, Weitzhandler, Isaac, Yingling, Yaroslava G., and Chilkoti, Ashutosh. Fri .
"Intrinsically disordered proteins access a range of hysteretic phase separation behaviors". United States. https://doi.org/10.1126/sciadv.aax5177. https://www.osti.gov/servlets/purl/1580951.
@article{osti_1580951,
title = {Intrinsically disordered proteins access a range of hysteretic phase separation behaviors},
author = {Garcia Quiroz, Felipe and Li, Nan K. and Roberts, Stefan and Weber, Patrick and Dzuricky, Michael and Weitzhandler, Isaac and Yingling, Yaroslava G. and Chilkoti, Ashutosh},
abstractNote = {The phase separation behavior of intrinsically disordered proteins (IDPs) is thought of as analogous to that of polymers that undergo equilibrium lower or upper critical solution temperature (LCST and UCST, respectively) phase transition. This view, however, ignores possible nonequilibrium properties of protein assemblies. Here, by studying IDP polymers (IDPPs) composed of repeat motifs that encode LCST or UCST phase behavior, we discovered that IDPs can access a wide spectrum of nonequilibrium, hysteretic phase behaviors. Experimentally and through simulations, we show that hysteresis in IDPPs is tunable and that it emerges through increasingly stable interchain interactions in the insoluble phase. To explore the utility of hysteretic IDPPs, we engineer self-assembling nanostructures with tunable stability. These findings shine light on the rich phase separation behavior of IDPs and illustrate hysteresis as a design parameter to program nonequilibrium phase behavior in self-assembling materials.},
doi = {10.1126/sciadv.aax5177},
journal = {Science Advances},
number = 10,
volume = 5,
place = {United States},
year = {Fri Oct 18 00:00:00 EDT 2019},
month = {Fri Oct 18 00:00:00 EDT 2019}
}
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Works referenced in this record:
A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins
journal, July 2018
- Wang, Jie; Choi, Jeong-Mo; Holehouse, Alex S.
- Cell, Vol. 174, Issue 3
Measuring the Elasticity of Poly- l -Proline Helices with Terahertz Spectroscopy
journal, April 2016
- Ruggiero, Michael T.; Sibik, Juraj; Orlando, Roberto
- Angewandte Chemie International Edition, Vol. 55, Issue 24
Rigidity, Secondary Structure, and the Universality of the Boson Peak in Proteins
journal, June 2014
- Perticaroli, Stefania; Nickels, Jonathan D.; Ehlers, Georg
- Biophysical Journal, Vol. 106, Issue 12
Thermoplastic Elastomer Hydrogels via Self-Assembly of an Elastin-Mimetic Triblock Polypeptide
journal, February 2002
- Wright, E. R.; McMillan, R. A.; Cooper, A.
- Advanced Functional Materials, Vol. 12, Issue 2
Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates
journal, January 2019
- Alberti, Simon; Gladfelter, Amy; Mittag, Tanja
- Cell, Vol. 176, Issue 3
Scalable molecular dynamics with NAMD
journal, January 2005
- Phillips, James C.; Braun, Rosemary; Wang, Wei
- Journal of Computational Chemistry, Vol. 26, Issue 16, p. 1781-1802
Clustering Molecular Dynamics Trajectories: 1. Characterizing the Performance of Different Clustering Algorithms
journal, October 2007
- Shao, Jianyin; Tanner, Stephen W.; Thompson, Nephi
- Journal of Chemical Theory and Computation, Vol. 3, Issue 6
Tunable, concentration-independent, sharp, hysteresis-free UCST phase transition from poly( N -acryloyl glycinamide-acrylonitrile) system
journal, October 2016
- Käfer, Florian; Lerch, Arne; Agarwal, Seema
- Journal of Polymer Science Part A: Polymer Chemistry, Vol. 55, Issue 2
In Vivo Formation of Vacuolated Multi-phase Compartments Lacking Membranes
journal, August 2016
- Schmidt, Hermann Broder; Rohatgi, Rajat
- Cell Reports, Vol. 16, Issue 5
Drug delivery to solid tumors by elastin-like polypeptides
journal, December 2010
- McDaniel, Jonathan R.; Callahan, Daniel J.; Chilkoti, Ashutosh
- Advanced Drug Delivery Reviews, Vol. 62, Issue 15
Accurate secondary structure prediction and fold recognition for circular dichroism spectroscopy
journal, June 2015
- Micsonai, András; Wien, Frank; Kernya, Linda
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 24
Poly( N -isopropylacrylamide) Phase Diagrams: Fifty Years of Research
journal, November 2015
- Halperin, Avraham; Kröger, Martin; Winnik, Françoise M.
- Angewandte Chemie International Edition, Vol. 54, Issue 51
Comparison of simple potential functions for simulating liquid water
journal, July 1983
- Jorgensen, William L.; Chandrasekhar, Jayaraman; Madura, Jeffry D.
- The Journal of Chemical Physics, Vol. 79, Issue 2
ALS/FTD Mutation-Induced Phase Transition of FUS Liquid Droplets and Reversible Hydrogels into Irreversible Hydrogels Impairs RNP Granule Function
journal, November 2015
- Murakami, Tetsuro; Qamar, Seema; Lin, Julie Qiaojin
- Neuron, Vol. 88, Issue 4
Phase transition of aqueous solutions of poly(N-isopropylacrylamide) and poly(N-isopropylmethacrylamide)
journal, April 1989
- Fujishige, Shouei; Kubota, K.; Ando, I.
- The Journal of Physical Chemistry, Vol. 93, Issue 8
Control of protein–ligand recognition using a stimuli-responsive polymer
journal, November 1995
- Stayton, Patrick S.; Shimoboji, Tsuyoshi; Long, Cynthia
- Nature, Vol. 378, Issue 6556
Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers
journal, September 2015
- Quiroz, Felipe García; Chilkoti, Ashutosh
- Nature Materials, Vol. 14, Issue 11
Purification of recombinant proteins by fusion with thermally-responsive polypeptides
journal, November 1999
- Meyer, Dan E.; Chilkoti, Ashutosh
- Nature Biotechnology, Vol. 17, Issue 11
Highly Ordered Polypeptide with UCST Phase Separation Behavior
journal, December 2018
- Kuroyanagi, Sotaro; Shimada, Naohiko; Fujii, Shota
- Journal of the American Chemical Society, Vol. 141, Issue 3
Injectable tissue integrating networks from recombinant polypeptides with tunable order
journal, October 2018
- Roberts, Stefan; Harmon, Tyler S.; Schaal, Jeffrey L.
- Nature Materials, Vol. 17, Issue 12
LCST and UCST in One: Double Thermoresponsive Behavior of Block Copolymers of Poly(ethylene glycol) and Poly(acrylamide -co- acrylonitrile)
journal, August 2015
- Käfer, Florian; Liu, Fangyao; Stahlschmidt, Ullrich
- Langmuir, Vol. 31, Issue 32
Engineering and characterization of a superfolder green fluorescent protein
journal, December 2005
- Pédelacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy
- Nature Biotechnology, Vol. 24, Issue 1, p. 79-88
Self-assembling elastin-like peptides growth factor chimeric nanoparticles for the treatment of chronic wounds
journal, December 2010
- Koria, P.; Yagi, H.; Kitagawa, Y.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 3
A highly parallel method for synthesizing DNA repeats enables the discovery of ‘smart’ protein polymers
journal, January 2011
- Amiram, Miriam; Quiroz, Felipe Garcia; Callahan, Daniel J.
- Nature Materials, Vol. 10, Issue 2
Phase Behavior and Self-Assembly of Perfectly Sequence-Defined and Monodisperse Multiblock Copolypeptides
journal, January 2017
- MacEwan, Sarah R.; Weitzhandler, Isaac; Hoffmann, Ingo
- Biomacromolecules, Vol. 18, Issue 2
Changes in the Hydration States of Poly( N - n -propylmethacrylamide) and Poly( N -isopropylmethacrylamide) during Their Phase Transitions in Water Observed by FTIR Spectroscopy
journal, November 2001
- Maeda, Yasushi; Nakamura, Tomoya; Ikeda, Isao
- Macromolecules, Vol. 34, Issue 23
Polymers with Upper Critical Solution Temperature in Aqueous Solution
journal, September 2012
- Seuring, Jan; Agarwal, Seema
- Macromolecular Rapid Communications, Vol. 33, Issue 22
Arrested Phase Separation of Elastin-like Polypeptide Solutions Yields Stiff, Thermoresponsive Gels
journal, November 2015
- Glassman, Matthew J.; Olsen, Bradley D.
- Biomacromolecules, Vol. 16, Issue 12
Sequence-Controlled Polymers
journal, August 2013
- Lutz, J. -F.; Ouchi, M.; Liu, D. R.
- Science, Vol. 341, Issue 6146
Recursive Directional Ligation by Plasmid Reconstruction Allows Rapid and Seamless Cloning of Oligomeric Genes
journal, April 2010
- McDaniel, Jonathan R.; MacKay, J. Andrew; Quiroz, Felipe García
- Biomacromolecules, Vol. 11, Issue 4
Investigating the Hydrogen-Bonding Model of Urea Denaturation
journal, July 2009
- Sagle, Laura B.; Zhang, Yanjie; Litosh, Vladislav A.
- Journal of the American Chemical Society, Vol. 131, Issue 26
Thermal Behavior and Kinetic Analysis of the Chain Unfolding and Refolding and of the Concomitant Nonpolar Solvation and Desolvation of Two Elastin-like Polymers
journal, November 2003
- Reguera, Javier; Lagarón, José M.; Alonso, Matilde
- Macromolecules, Vol. 36, Issue 22
Corneal Reconstruction with Tissue-Engineered Cell Sheets Composed of Autologous Oral Mucosal Epithelium
journal, September 2004
- Nishida, Kohji; Yamato, Masayuki; Hayashida, Yasutaka
- New England Journal of Medicine, Vol. 351, Issue 12
Synthesis and properties of crosslinked recombinant pro-resilin
journal, October 2005
- Elvin, Christopher M.; Carr, Andrew G.; Huson, Mickey G.
- Nature, Vol. 437, Issue 7061
Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets
journal, January 2017
- Shin, Yongdae; Berry, Joel; Pannucci, Nicole
- Cell, Vol. 168, Issue 1-2
Sequence Directionality Dramatically Affects LCST Behavior of Elastin-Like Polypeptides
journal, April 2018
- Li, Nan K.; Roberts, Stefan; Quiroz, Felipe Garcia
- Biomacromolecules, Vol. 19, Issue 7
Proline and Glycine Control Protein Self-Organization into Elastomeric or Amyloid Fibrils
journal, November 2006
- Rauscher, Sarah; Baud, Stéphanie; Miao, Ming
- Structure, Vol. 14, Issue 11
Molecular Description of the LCST Behavior of an Elastin-Like Polypeptide
journal, September 2014
- Li, Nan K.; Quiroz, Felipe García; Hall, Carol K.
- Biomacromolecules, Vol. 15, Issue 10
A Genetically Engineered Protein Responsive to Multiple Stimuli
journal, April 2011
- Dutta, Naba K.; Truong, My Y.; Mayavan, Sundar
- Angewandte Chemie International Edition, Vol. 50, Issue 19
Non-equilibrium supramolecular polymerization
journal, January 2017
- Sorrenti, Alessandro; Leira-Iglesias, Jorge; Markvoort, Albert J.
- Chemical Society Reviews, Vol. 46, Issue 18
Chirality-selected phase behaviour in ionic polypeptide complexes
journal, January 2015
- Perry, Sarah L.; Leon, Lorraine; Hoffmann, Kyle Q.
- Nature Communications, Vol. 6, Issue 1
Temperature Triggered Self-Assembly of Polypeptides into Multivalent Spherical Micelles
journal, January 2008
- Dreher, Matthew R.; Simnick, Andrew J.; Fischer, Karl
- Journal of the American Chemical Society, Vol. 130, Issue 2
Emerging applications of stimuli-responsive polymer materials
journal, January 2010
- Stuart, Martien A. Cohen; Huck, Wilhelm T. S.; Genzer, Jan
- Nature Materials, Vol. 9, Issue 2, p. 101-113
The role of proline substitutions within flexible regions on thermostability of luciferase
journal, January 2015
- Yu, Haoran; Zhao, Yang; Guo, Chao
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1854, Issue 1
Polypeptide self-assemblies: nanostructures and bioapplications
journal, January 2016
- Cai, Chunhua; Lin, Jiaping; Lu, Yingqing
- Chemical Society Reviews, Vol. 45, Issue 21
First Example of a Universal and Cost-Effective Approach: Polymers with Tunable Upper Critical Solution Temperature in Water and Electrolyte Solution
journal, April 2012
- Seuring, Jan; Agarwal, Seema
- Macromolecules, Vol. 45, Issue 9
Genetically encoded lipid–polypeptide hybrid biomaterials that exhibit temperature-triggered hierarchical self-assembly
journal, March 2018
- Mozhdehi, Davoud; Luginbuhl, Kelli M.; Simon, Joseph R.
- Nature Chemistry, Vol. 10, Issue 5
Shape effects of filaments versus spherical particles in flow and drug delivery
journal, March 2007
- Geng, Yan; Dalhaimer, Paul; Cai, Shenshen
- Nature Nanotechnology, Vol. 2, Issue 4
Biomolecular condensates: organizers of cellular biochemistry
journal, February 2017
- Banani, Salman F.; Lee, Hyun O.; Hyman, Anthony A.
- Nature Reviews Molecular Cell Biology, Vol. 18, Issue 5
Thermosensitive sol–gel reversible hydrogels
journal, January 2002
- Jeong, Byeongmoon; Kim, Sung Wan; Bae, You Han
- Advanced Drug Delivery Reviews, Vol. 54, Issue 1
Using circular dichroism spectra to estimate protein secondary structure
journal, January 2007
- Greenfield, Norma J
- Nature Protocols, Vol. 1, Issue 6, p. 2876-2890
Thermosensitive sol–gel reversible hydrogels
journal, December 2012
- Jeong, Byeongmoon; Kim, Sung Wan; Bae, You Han
- Advanced Drug Delivery Reviews, Vol. 64
Pol II phosphorylation regulates a switch between transcriptional and splicing condensates
journal, August 2019
- Guo, Yang Eric; Manteiga, John C.; Henninger, Jonathan E.
- Nature, Vol. 572, Issue 7770
Measuring the Elasticity of Poly- l -Proline Helices with Terahertz Spectroscopy
journal, April 2016
- Ruggiero, Michael T.; Sibik, Juraj; Orlando, Roberto
- Angewandte Chemie International Edition, Vol. 55, Issue 24
Scalable molecular dynamics with NAMD
journal, January 2005
- Phillips, James C.; Braun, Rosemary; Wang, Wei
- Journal of Computational Chemistry, Vol. 26, Issue 16, p. 1781-1802
Polymers with Upper Critical Solution Temperature in Aqueous Solution
journal, September 2012
- Seuring, Jan; Agarwal, Seema
- Macromolecular Rapid Communications, Vol. 33, Issue 22
Thermosensitive sol–gel reversible hydrogels
journal, December 2012
- Jeong, Byeongmoon; Kim, Sung Wan; Bae, You Han
- Advanced Drug Delivery Reviews, Vol. 64
Rigidity, Secondary Structure, and the Universality of the Boson Peak in Proteins
journal, June 2014
- Perticaroli, Stefania; Nickels, Jonathan D.; Ehlers, Georg
- Biophysical Journal, Vol. 106, Issue 12
A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins
journal, July 2018
- Wang, Jie; Choi, Jeong-Mo; Holehouse, Alex S.
- Cell, Vol. 174, Issue 3
Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates
journal, January 2019
- Alberti, Simon; Gladfelter, Amy; Mittag, Tanja
- Cell, Vol. 176, Issue 3
In Vivo Formation of Vacuolated Multi-phase Compartments Lacking Membranes
journal, August 2016
- Schmidt, Hermann Broder; Rohatgi, Rajat
- Cell Reports, Vol. 16, Issue 5
Proline and Glycine Control Protein Self-Organization into Elastomeric or Amyloid Fibrils
journal, November 2006
- Rauscher, Sarah; Baud, Stéphanie; Miao, Ming
- Structure, Vol. 14, Issue 11
Sequence Directionality Dramatically Affects LCST Behavior of Elastin-Like Polypeptides
journal, April 2018
- Li, Nan K.; Roberts, Stefan; Quiroz, Felipe Garcia
- Biomacromolecules, Vol. 19, Issue 7
LCST and UCST in One: Double Thermoresponsive Behavior of Block Copolymers of Poly(ethylene glycol) and Poly(acrylamide -co- acrylonitrile)
journal, August 2015
- Käfer, Florian; Liu, Fangyao; Stahlschmidt, Ullrich
- Langmuir, Vol. 31, Issue 32
Molecular Description of the LCST Behavior of an Elastin-Like Polypeptide
journal, September 2014
- Li, Nan K.; Quiroz, Felipe García; Hall, Carol K.
- Biomacromolecules, Vol. 15, Issue 10
Recursive Directional Ligation by Plasmid Reconstruction Allows Rapid and Seamless Cloning of Oligomeric Genes
journal, April 2010
- McDaniel, Jonathan R.; MacKay, J. Andrew; Quiroz, Felipe García
- Biomacromolecules, Vol. 11, Issue 4
Clustering Molecular Dynamics Trajectories: 1. Characterizing the Performance of Different Clustering Algorithms
journal, October 2007
- Shao, Jianyin; Tanner, Stephen W.; Thompson, Nephi
- Journal of Chemical Theory and Computation, Vol. 3, Issue 6
Phase transition of aqueous solutions of poly(N-isopropylacrylamide) and poly(N-isopropylmethacrylamide)
journal, April 1989
- Fujishige, Shouei; Kubota, K.; Ando, I.
- The Journal of Physical Chemistry, Vol. 93, Issue 8
Temperature Triggered Self-Assembly of Polypeptides into Multivalent Spherical Micelles
journal, January 2008
- Dreher, Matthew R.; Simnick, Andrew J.; Fischer, Karl
- Journal of the American Chemical Society, Vol. 130, Issue 2
Investigating the Hydrogen-Bonding Model of Urea Denaturation
journal, July 2009
- Sagle, Laura B.; Zhang, Yanjie; Litosh, Vladislav A.
- Journal of the American Chemical Society, Vol. 131, Issue 26
Highly Ordered Polypeptide with UCST Phase Separation Behavior
journal, December 2018
- Kuroyanagi, Sotaro; Shimada, Naohiko; Fujii, Shota
- Journal of the American Chemical Society, Vol. 141, Issue 3
Thermal Behavior and Kinetic Analysis of the Chain Unfolding and Refolding and of the Concomitant Nonpolar Solvation and Desolvation of Two Elastin-like Polymers
journal, November 2003
- Reguera, Javier; Lagarón, José M.; Alonso, Matilde
- Macromolecules, Vol. 36, Issue 22
First Example of a Universal and Cost-Effective Approach: Polymers with Tunable Upper Critical Solution Temperature in Water and Electrolyte Solution
journal, April 2012
- Seuring, Jan; Agarwal, Seema
- Macromolecules, Vol. 45, Issue 9
Control of protein–ligand recognition using a stimuli-responsive polymer
journal, November 1995
- Stayton, Patrick S.; Shimoboji, Tsuyoshi; Long, Cynthia
- Nature, Vol. 378, Issue 6556
Synthesis and properties of crosslinked recombinant pro-resilin
journal, October 2005
- Elvin, Christopher M.; Carr, Andrew G.; Huson, Mickey G.
- Nature, Vol. 437, Issue 7061
Correction: Corrigendum: Engineering and characterization of a superfolder green fluorescent protein
journal, September 2006
- Pe´delacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy
- Nature Biotechnology, Vol. 24, Issue 9
Engineering and characterization of a superfolder green fluorescent protein
journal, December 2005
- Pédelacq, Jean-Denis; Cabantous, Stéphanie; Tran, Timothy
- Nature Biotechnology, Vol. 24, Issue 1, p. 79-88
Chirality-selected phase behaviour in ionic polypeptide complexes
journal, January 2015
- Perry, Sarah L.; Leon, Lorraine; Hoffmann, Kyle Q.
- Nature Communications, Vol. 6, Issue 1
Emerging applications of stimuli-responsive polymer materials
journal, January 2010
- Stuart, Martien A. Cohen; Huck, Wilhelm T. S.; Genzer, Jan
- Nature Materials, Vol. 9, Issue 2, p. 101-113
Shape effects of filaments versus spherical particles in flow and drug delivery
journal, March 2007
- Geng, Yan; Dalhaimer, Paul; Cai, Shenshen
- Nature Nanotechnology, Vol. 2, Issue 4
Biomolecular condensates: organizers of cellular biochemistry
journal, February 2017
- Banani, Salman F.; Lee, Hyun O.; Hyman, Anthony A.
- Nature Reviews Molecular Cell Biology, Vol. 18, Issue 5
Changing the Cell Source in Cell Therapy?
journal, September 2004
- Pellegrini, Graziella
- New England Journal of Medicine, Vol. 351, Issue 12
Self-assembling elastin-like peptides growth factor chimeric nanoparticles for the treatment of chronic wounds
journal, December 2010
- Koria, P.; Yagi, H.; Kitagawa, Y.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 3
Accurate secondary structure prediction and fold recognition for circular dichroism spectroscopy
journal, June 2015
- Micsonai, András; Wien, Frank; Kernya, Linda
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 24
Sequence-Controlled Polymers
journal, August 2013
- Lutz, J. -F.; Ouchi, M.; Liu, D. R.
- Science, Vol. 341, Issue 6146
Works referencing / citing this record:
Unification of lower and upper critical solution temperature phase behavior of globular protein solutions in the presence of multivalent cations
journal, January 2020
- Begam, Nafisa; Matsarskaia, Olga; Sztucki, Michael
- Soft Matter, Vol. 16, Issue 8