Integrated Bioinformatic Analyses and Immune Characterization of New Neisseria gonorrhoeae Vaccine Antigens Expressed during Natural Mucosal Infection
Abstract
There is an increasingly severe trend of antibiotic-resistant Neisseria gonorrhoeae strains worldwide and new therapeutic strategies are needed against this sexually-transmitted pathogen. Despite the urgency, progress towards a gonococcal vaccine has been slowed by a scarcity of suitable antigens, lack of correlates of protection in humans and limited animal models of infection. N. gonorrhoeae gene expression levels in the natural human host does not reflect expression in vitro, further complicating in vitro-basedvaccine analysis platforms. We designed a novel candidate antigen selection strategy (CASS), based on a reverse vaccinology-like approach coupled with bioinformatics. We utilized the CASS to mine gonococcal proteins expressed during human mucosal infection, reported in our previous studies, and focused on a large pool of hypothetical proteins as an untapped source of potential new antigens. Via two discovery and analysis phases (DAP), we identified 36 targets predicted to be immunogenic, membrane-associated proteins conserved in N. gonorrhoeae and suitable for recombinant expression. Six initial candidates were produced and used to immunize mice. Characterization of the immune responses indicated cross-reactive antibodies and serum bactericidal activity against different N. gonorrhoeae strains. These results support the CASS as a tool for the discovery of new vaccine candidates.
- Authors:
-
[3];
[1]
- Tufts Univ. School of Medicine, Boston, MA (United States). Dept. of Immunology
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Div.
- Univ. of Oxford (United Kingdom). Dept. of Zoology
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1574883
- Report Number(s):
- PNNL-SA-146316
Journal ID: ISSN 2076-393X; VBSABP
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Vaccines
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 4; Journal ID: ISSN 2076-393X
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; gonorrhea; vaccine; in silicoanalysis; hypothetical proteins; bactericidal antibodies
Citation Formats
Zhu, Tianmou, McClure, Ryan S., Harrison, Odile B., Genco, Caroline, and Massari, Paola. Integrated Bioinformatic Analyses and Immune Characterization of New Neisseria gonorrhoeae Vaccine Antigens Expressed during Natural Mucosal Infection. United States: N. p., 2019.
Web. doi:10.3390/vaccines7040153.
Zhu, Tianmou, McClure, Ryan S., Harrison, Odile B., Genco, Caroline, & Massari, Paola. Integrated Bioinformatic Analyses and Immune Characterization of New Neisseria gonorrhoeae Vaccine Antigens Expressed during Natural Mucosal Infection. United States. https://doi.org/10.3390/vaccines7040153
Zhu, Tianmou, McClure, Ryan S., Harrison, Odile B., Genco, Caroline, and Massari, Paola. Thu .
"Integrated Bioinformatic Analyses and Immune Characterization of New Neisseria gonorrhoeae Vaccine Antigens Expressed during Natural Mucosal Infection". United States. https://doi.org/10.3390/vaccines7040153. https://www.osti.gov/servlets/purl/1574883.
@article{osti_1574883,
title = {Integrated Bioinformatic Analyses and Immune Characterization of New Neisseria gonorrhoeae Vaccine Antigens Expressed during Natural Mucosal Infection},
author = {Zhu, Tianmou and McClure, Ryan S. and Harrison, Odile B. and Genco, Caroline and Massari, Paola},
abstractNote = {There is an increasingly severe trend of antibiotic-resistant Neisseria gonorrhoeae strains worldwide and new therapeutic strategies are needed against this sexually-transmitted pathogen. Despite the urgency, progress towards a gonococcal vaccine has been slowed by a scarcity of suitable antigens, lack of correlates of protection in humans and limited animal models of infection. N. gonorrhoeae gene expression levels in the natural human host does not reflect expression in vitro, further complicating in vitro-basedvaccine analysis platforms. We designed a novel candidate antigen selection strategy (CASS), based on a reverse vaccinology-like approach coupled with bioinformatics. We utilized the CASS to mine gonococcal proteins expressed during human mucosal infection, reported in our previous studies, and focused on a large pool of hypothetical proteins as an untapped source of potential new antigens. Via two discovery and analysis phases (DAP), we identified 36 targets predicted to be immunogenic, membrane-associated proteins conserved in N. gonorrhoeae and suitable for recombinant expression. Six initial candidates were produced and used to immunize mice. Characterization of the immune responses indicated cross-reactive antibodies and serum bactericidal activity against different N. gonorrhoeae strains. These results support the CASS as a tool for the discovery of new vaccine candidates.},
doi = {10.3390/vaccines7040153},
journal = {Vaccines},
number = 4,
volume = 7,
place = {United States},
year = {Thu Oct 17 00:00:00 EDT 2019},
month = {Thu Oct 17 00:00:00 EDT 2019}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 6 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
The gene bglH present in the bgl operon of Escherichia coli, responsible for uptake and fermentation of beta-glucosides encodes for a carbohydrate-specific outer membrane porin
journal, January 1999
- Andersen, Christian; Rak, Bodo; Benz, Roland
- Molecular Microbiology, Vol. 31, Issue 2
Experimental vaccine induces Th1-driven immune responses and resistance to Neisseria gonorrhoeae infection in a murine model
text, January 2017
- ,
- The University of North Carolina at Chapel Hill University Libraries
Gonorrhoea reinfection in heterosexual STD clinic attendees: longitudinal analysis of risks for first reinfection
journal, April 2003
- Mehta, S. D.
- Sexually Transmitted Infections, Vol. 79, Issue 2
Subversion of nutritional immunity by the pathogenic Neisseriae
journal, October 2017
- Cornelissen, Cynthia Nau
- Pathogens and Disease, Vol. 76, Issue 1
The  -Barrel Outer Membrane Protein Assembly Complex of Neisseria meningitidis
journal, September 2009
- Volokhina, E. B.; Beckers, F.; Tommassen, J.
- Journal of Bacteriology, Vol. 191, Issue 22
KEGG: Kyoto Encyclopedia of Genes and Genomes
journal, January 2000
- Kanehisa, Minoru; Goto, Susumu
- Nucleic Acids Research, Vol. 28, Issue 1, p. 27-30
Fur-Mediated Global Regulatory Circuits in Pathogenic Neisseria Species
journal, August 2012
- Yu, Chunxiao; Genco, Caroline Attardo
- Journal of Bacteriology, Vol. 194, Issue 23
A Meningococcal Native Outer Membrane Vesicle Vaccine With Attenuated Endotoxin and Overexpressed Factor H Binding Protein Elicits Gonococcal Bactericidal Antibodies
journal, October 2018
- Beernink, Peter T.; Ispasanie, Emma; Lewis, Lisa A.
- The Journal of Infectious Diseases, Vol. 219, Issue 7
The PorB porin from commensal Neisseria lactamica induces Th1 and Th2 immune responses to ovalbumin in mice and is a potential immune adjuvant
journal, February 2008
- Liu, Xiuping; Wetzler, Lee M.; Massari, Paola
- Vaccine, Vol. 26, Issue 6
Feature-based prediction of non-classical and leaderless protein secretion
journal, May 2004
- Bendtsen, J. D.; Jensen, L. J.; Blom, N.
- Protein Engineering Design and Selection, Vol. 17, Issue 4
Experimental vaccine induces Th1-driven immune responses and resistance to Neisseria gonorrhoeae infection in a murine model
journal, March 2017
- Liu, Y.; Hammer, L. A.; Liu, W.
- Mucosal Immunology, Vol. 10, Issue 6
Bacterial copper storage proteins
journal, February 2018
- Dennison, Christopher; David, Sholto; Lee, Jaeick
- Journal of Biological Chemistry, Vol. 293, Issue 13
Targeting Lipooligosaccharide (LOS) for a Gonococcal Vaccine
journal, February 2019
- Gulati, Sunita; Shaughnessy, Jutamas; Ram, Sanjay
- Frontiers in Immunology, Vol. 10
Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection
journal, November 1999
- Seydel, Anke; Gounon, Pierre; Pugsley, Anthony P.
- Molecular Microbiology, Vol. 34, Issue 4
Innate Nutritional Immunity
journal, June 2018
- Núñez, Gabriel; Sakamoto, Kei; Soares, Miguel P.
- The Journal of Immunology, Vol. 201, Issue 1
Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display
journal, February 2016
- Connor, Daniel O.; Zantow, Jonas; Hust, Michael
- PLOS ONE, Vol. 11, Issue 2
Dynamic Association of BAM Complex Modules Includes Surface Exposure of the Lipoprotein BamC
journal, September 2012
- Webb, Chaille T.; Selkrig, Joel; Perry, Andrew J.
- Journal of Molecular Biology, Vol. 422, Issue 4
BIGSdb: Scalable analysis of bacterial genome variation at the population level
journal, December 2010
- Jolley, Keith A.; Maiden, Martin CJ
- BMC Bioinformatics, Vol. 11, Issue 1
SignalP 5.0 improves signal peptide predictions using deep neural networks
journal, February 2019
- Almagro Armenteros, José Juan; Tsirigos, Konstantinos D.; Sønderby, Casper Kaae
- Nature Biotechnology, Vol. 37, Issue 4
Prediction of lipoprotein signal peptides in Gram-negative bacteria
journal, August 2003
- Juncker, Agnieszka S.; Willenbrock, Hanni; von Heijne, Gunnar
- Protein Science, Vol. 12, Issue 8
The Gonococcal Transcriptome during Infection of the Lower Genital Tract in Women
journal, August 2015
- McClure, Ryan; Nudel, Kathleen; Massari, Paola
- PLOS ONE, Vol. 10, Issue 8
A universal vaccine for serogroup B meningococcus
journal, July 2006
- Giuliani, M. M.; Adu-Bobie, J.; Comanducci, M.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 29
In-silico Hierarchical Approach for the Identification of Potential Universal Vaccine Candidates (PUVCs) from Neisseria gonorrhoeae
journal, December 2016
- Jain, Ravi; Sonkar, Subash C.; Chaudhry, Uma
- Journal of Theoretical Biology, Vol. 410
The novel interaction between Neisseria gonorrhoeae TdfJ and human S100A7 allows gonococci to subvert host zinc restriction
journal, August 2019
- Maurakis, Stavros; Keller, Kayla; Maxwell, C. Noel
- PLOS Pathogens, Vol. 15, Issue 8
VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines
journal, January 2007
- Doytchinova, Irini A.; Flower, Darren R.
- BMC Bioinformatics, Vol. 8, Issue 1
The reproductive cycle is a pathogenic determinant during gonococcal pelvic inflammatory disease in mice
journal, December 2015
- Islam, E. A.; Shaik-Dasthagirisaheb, Y.; Kaushic, C.
- Mucosal Immunology, Vol. 9, Issue 4
Structural and Immunological Characterization of Novel Recombinant MOMP-Based Chlamydial Antigens
journal, December 2017
- Madico, Guillermo; Gursky, Olga; Fairman, Jeff
- Vaccines, Vol. 6, Issue 1
Neisserial surface lipoproteins: structure, function and biogenesis
journal, February 2017
- Hooda, Yogesh; Shin, Hyejin E.; Bateman, Thomas J.
- Pathogens and Disease, Vol. 75, Issue 2
Proteomic and Transcriptomic Analyses Reveal Pathological Changes in the Entorhinal Cortex Region that Correlate Well with Dysregulation of Ion Transport in Patients with Alzheimer’s Disease
journal, April 2021
- Jia, Yangjie; Wang, Xia; Chen, Yanyu
- Molecular Neurobiology, Vol. 58, Issue 8
Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254
journal, July 2006
- Vipond, Caroline; Suker, Janet; Jones, Christopher
- PROTEOMICS, Vol. 6, Issue 14
Intranasal Immunization with Gonococcal Outer Membrane Preparations Reduces the Duration of Vaginal Colonization of Mice by Neisseria gonorrhoeae
journal, September 2000
- Plante, Martin; Jerse, Ann; Hamel, Josée
- The Journal of Infectious Diseases, Vol. 182, Issue 3
Experimental Gonococcal Infection in Male Volunteers: Cumulative Experience with Neisseria gonorrhoeae Strains FA1090 and MS11mkC
text, January 2011
- M., Hobbs, Marcia; M., Shafer, William; Frederick, Sparling, P.
- The University of North Carolina at Chapel Hill University Libraries
Recombinant bacterial lipoproteins as vaccine candidates
journal, September 2015
- Leng, Chih-Hsiang; Liu, Shih-Jen; Chen, Hsin-Wei
- Expert Review of Vaccines, Vol. 14, Issue 12
The Serogroup B Meningococcal Vaccine Bexsero Elicits Antibodies to Neisseria gonorrhoeae
journal, December 2018
- Semchenko, Evgeny A.; Tan, Aimee; Borrow, Ray
- Clinical Infectious Diseases, Vol. 69, Issue 7
Identification of new meningococcal serogroup B surface antigens through a systematic analysis of neisserial genomes
journal, December 2009
- Pajon, Rolando; Yero, Daniel; Niebla, Olivia
- Vaccine, Vol. 28, Issue 2
Integrated in silico Analyses of Regulatory and Metabolic Networks of Synechococcus sp. PCC 7002 Reveal Relationships between Gene Centrality and Essentiality
journal, March 2015
- Song, Hyun-Seob; McClure, Ryan; Bernstein, Hans
- Life, Vol. 5, Issue 2
Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen
journal, January 2001
- Krogh, Anders; Larsson, Björn; von Heijne, Gunnar
- Journal of Molecular Biology, Vol. 305, Issue 3
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
DeepLoc: prediction of protein subcellular localization using deep learning
journal, July 2017
- Almagro Armenteros, José Juan; Sønderby, Casper Kaae; Sønderby, Søren Kaae
- Bioinformatics, Vol. 33, Issue 21
Identification of novel immunogenic proteins of Neisseria gonorrhoeae by phage display
text, January 2019
- Connor, Daniel Oliver; Zantow, Jonas; Hust, Michael
- Universität Potsdam
Effectiveness of a Group B Outer Membrane Vesicle Meningococcal Vaccine in Preventing Hospitalization from Gonorrhea in New Zealand: A Retrospective Cohort Study
journal, January 2019
- Paynter, Janine; Goodyear-Smith, Felicity; Morgan, Jane
- Vaccines, Vol. 7, Issue 1
Copper(II)-Induced Restructuring of ZnuD, a Zinc(II) Transporter from Neisseria meningitidis
journal, April 2019
- Hecel, Aleksandra; Rowińska-Żyrek, Magdalena; Kozłowski, Henryk
- Inorganic Chemistry, Vol. 58, Issue 9
Lipoprotein NMB0928 from Neisseria meningitidis serogroup B as a novel vaccine candidate
journal, December 2007
- Delgado, Maité; Yero, Daniel; Niebla, Olivia
- Vaccine, Vol. 25, Issue 50
Environmental dissemination of pathogenic Listeria monocytogenes in flowing surface waters in Switzerland
journal, April 2021
- Raschle, Susanne; Stephan, Roger; Stevens, Marc J. A.
- Scientific Reports, Vol. 11, Issue 1
PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes
journal, May 2010
- Yu, Nancy Y.; Wagner, James R.; Laird, Matthew R.
- Bioinformatics, Vol. 26, Issue 13
Identification of Vaccine Candidates Against Serogroup B Meningococcus by Whole-Genome Sequencing
journal, March 2000
- Pizza, M.
- Science, Vol. 287, Issue 5459
Experimental Gonococcal Urethritis and Reinfection with Homologous Gonococci in Male Volunteers
journal, January 2001
- Schmidt, Katherine A.; Schneider, Herman; Lindstrom, Jill A.
- Sexually Transmitted Diseases, Vol. 28, Issue 10
Insights into the regulation of protein abundance from proteomic and transcriptomic analyses
journal, March 2012
- Vogel, Christine; Marcotte, Edward M.
- Nature Reviews Genetics, Vol. 13, Issue 4
Gneg-mPLoc: A top-down strategy to enhance the quality of predicting subcellular localization of Gram-negative bacterial proteins
journal, May 2010
- Shen, Hong-Bin; Chou, Kuo-Chen
- Journal of Theoretical Biology, Vol. 264, Issue 2
Recombinant protein meningococcal serogroup B vaccine combined with outer membrane vesicles
journal, May 2011
- Bai, Xilian; Findlow, Jamie; Borrow, Ray
- Expert Opinion on Biological Therapy, Vol. 11, Issue 7
Antimicrobial susceptibility of Neisseria gonorrhoeaeisolates from symptomatic men attending the Nanjing sexually transmitted diseases clinic (2011–2012): genetic characteristics of isolates with reduced sensitivity to ceftriaxone
journal, November 2014
- Li, Sai; Su, Xiao-Hong; Le, Wen-Jing
- BMC Infectious Diseases, Vol. 14, Issue 1
Topological analysis of protein co-abundance networks identifies novel host targets important for HCV infection and pathogenesis
journal, January 2012
- McDermott, Jason E.; Diamond, Deborah L.; Corley, Courtney
- BMC Systems Biology, Vol. 6, Issue 1
Antimicrobial Resistance Expressed by Neisseria gonorrhoeae: A Major Global Public Health Problem in the 21st Century
journal, June 2016
- Unemo, Magnus; del Rio, Carlos; Shafer, William M.
- Microbiology Spectrum, Vol. 4, Issue 3
Effectiveness of a group B outer membrane vesicle meningococcal vaccine against gonorrhoea in New Zealand: a retrospective case-control study
journal, September 2017
- Petousis-Harris, Helen; Paynter, Janine; Morgan, Jane
- The Lancet, Vol. 390, Issue 10102
Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae
journal, February 2009
- Vik, A.; Aas, F. E.; Anonsen, J. H.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 11
A genetic screen identifies genes and sites involved in pilin antigenic variation in Neisseria gonorrhoeae : Gonococcal mutants with impaired pilin variation
journal, June 2005
- Sechman, Eric V.; Rohrer, Melissa S.; Seifert, H. Steven
- Molecular Microbiology, Vol. 57, Issue 2
Antibody to Rmp (outer membrane protein 3) increases susceptibility to gonococcal infection.
journal, January 1993
- Plummer, F. A.; Chubb, H.; Simonsen, J. N.
- Journal of Clinical Investigation, Vol. 91, Issue 1
Neisseria gonorrhoeae : Drug Resistance, Mouse Models, and Vaccine Development
journal, September 2017
- Rice, Peter A.; Shafer, William M.; Ram, Sanjay
- Annual Review of Microbiology, Vol. 71, Issue 1
Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species
journal, February 2019
- Fegan, Jamie E.; Calmettes, Charles; Islam, Epshita A.
- Frontiers in Immunology, Vol. 10
Regulation of IL-10 and IL-12 production and function in macrophages and dendritic cells
journal, January 2015
- Ma, Xiaojing; Yan, Wenjun; Zheng, Hua
- F1000Research, Vol. 4
Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254
journal, June 2006
- Vipond, Caroline; Suker, Janet; Jones, Christopher
- PROTEOMICS, Vol. 6, Issue 11
Meningococcal B vaccine (4CMenB): the journey from research to real world experience
journal, December 2018
- Rappuoli, Rino; Pizza, Mariagrazia; Masignani, Vega
- Expert Review of Vaccines, Vol. 17, Issue 12
Advantages of combined transmembrane topology and signal peptide prediction--the Phobius web server
journal, May 2007
- Kall, L.; Krogh, A.; Sonnhammer, E. L. L.
- Nucleic Acids Research, Vol. 35, Issue Web Server
PredictProtein—an open resource for online prediction of protein structural and functional features
journal, May 2014
- Yachdav, Guy; Kloppmann, Edda; Kajan, Laszlo
- Nucleic Acids Research, Vol. 42, Issue W1
The Lst Sialyltransferase of Neisseria gonorrhoeae Can Transfer Keto-Deoxyoctanoate as the Terminal Sugar of Lipooligosaccharide: a Glyco-Achilles Heel That Provides a New Strategy for Vaccines to Prevent Gonorrhea
journal, April 2021
- Jen, Freda E. -C.; Ketterer, Margaret R.; Semchenko, Evgeny A.
- mBio, Vol. 12, Issue 2
Molecular Typing of Neisseria gonorrhoeae Causing Repeated Infections: Evolution of Porin during Passage within a Community
journal, February 1999
- Hobbs, Marcia M.; Alcorn, Timothy M.; Davis, Rachael H.
- The Journal of Infectious Diseases, Vol. 179, Issue 2
Visual gene-network analysis reveals the cancer gene co-expression in human endometrial cancer
journal, January 2014
- Chou, Wei-Chun; Cheng, An-Lin; Brotto, Marco
- BMC Genomics, Vol. 15, Issue 1
Estradiol-Treated Female Mice as Surrogate Hosts for Neisseria gonorrhoeae Genital Tract Infections
journal, January 2011
- Jerse, Ann E.; Wu, Hong; Packiam, Mathanraj
- Frontiers in Microbiology, Vol. 2
The new multicomponent vaccine against meningococcal serogroup B, 4CMenB: Immunological, functional and structural characterization of the antigens
journal, May 2012
- Serruto, Davide; Bottomley, Matthew J.; Ram, Sanjay
- Vaccine, Vol. 30
Gonococcal pilin variants in experimental gonorrhea
journal, May 1987
- Swanson, J.
- Journal of Experimental Medicine, Vol. 165, Issue 5
Binding of C4b-Binding Protein to Porin: A Molecular Mechanism of Serum Resistance of
journal, January 2001
- Ram, Sanjay; Cullinane, Meabh; Blom, Anna M.
- The Journal of Experimental Medicine, Vol. 193, Issue 3
The Pfam protein families database in 2019
journal, October 2018
- El-Gebali, Sara; Mistry, Jaina; Bateman, Alex
- Nucleic Acids Research, Vol. 47, Issue D1
Copper tolerance and virulence in bacteria
journal, January 2015
- Ladomersky, Erik; Petris, Michael J.
- Metallomics, Vol. 7, Issue 6
The ironclad truth: how in vivo transcriptomics and in vitro mechanistic studies shape our understanding of Neisseria gonorrhoeae gene regulation during mucosal infection
journal, May 2017
- Moreau, Matthew R.; Massari, Paola; Genco, Caroline A.
- Pathogens and Disease, Vol. 75, Issue 5
UniProt: a worldwide hub of protein knowledge
November 2018
- Consortium, The UniProt
- Nucleic Acids Research
Updates on the web-based VIOLIN vaccine database and analysis system
journal, November 2013
- He, Yongqun; Racz, Rebecca; Sayers, Samantha
- Nucleic Acids Research, Vol. 42, Issue D1
Gonococcal transferrin binding protein chimeras induce bactericidal and growth inhibitory antibodies in mice
journal, October 2007
- Price, Gregory A.; Masri, Heather P.; Hollander, Aimee M.
- Vaccine, Vol. 25, Issue 41
Proteomics, Bioinformatics and Structure-Function Antigen Mining For Gonorrhea Vaccines
journal, December 2018
- Baarda, Benjamin I.; Martinez, Fabian G.; Sikora, Aleksandra E.
- Frontiers in Immunology, Vol. 9
Nasal Inoculation of the Commensal Neisseria lactamica Inhibits Carriage of Neisseria meningitidis by Young Adults: A Controlled Human Infection Study
journal, April 2015
- Deasy, Alice M.; Guccione, Ed; Dale, Adam P.
- Clinical Infectious Diseases, Vol. 60, Issue 10
Suppression of host adaptive immune responses by Neisseria gonorrhoeae: role of interleukin 10 and type 1 regulatory T cells
journal, June 2013
- Liu, Y.; Liu, W.; Russell, M. W.
- Mucosal Immunology, Vol. 7, Issue 1
Pathogenesis of Neisseria gonorrhoeae and the Host Defense in Ascending Infections of Human Fallopian Tube
journal, November 2018
- Lenz, Jonathan D.; Dillard, Joseph P.
- Frontiers in Immunology, Vol. 9
Broad vaccine coverage predicted for a bivalent recombinant factor H binding protein based vaccine to prevent serogroup B meningococcal disease
journal, August 2010
- Jiang, Han-Qing; Hoiseth, Susan K.; Harris, Shannon L.
- Vaccine, Vol. 28, Issue 37
From Chaperones to the Membrane with a BAM!
journal, October 2016
- Plummer, Ashlee M.; Fleming, Karen G.
- Trends in Biochemical Sciences, Vol. 41, Issue 10
A genetic screen reveals a periplasmic copper chaperone required for nitrite reductase activity in pathogenic Neisseria
journal, September 2015
- Jen, Freda E. -C.; Djoko, Karrera Y.; Bent, Stephen J.
- The FASEB Journal, Vol. 29, Issue 9
Immunogenicity profiling of protein antigens from capsular group B Neisseria meningitidis
journal, May 2019
- Awanye, Amaka M.; Chang, Chun-Mien; Wheeler, Jun X.
- Scientific Reports, Vol. 9, Issue 1
Neisseria meningitidis: pathogenesis and immunity
journal, February 2015
- Pizza, Mariagrazia; Rappuoli, Rino
- Current Opinion in Microbiology, Vol. 23
Immunoglobulin G antibodies directed against protein III block killing of serum-resistant Neisseria gonorrhoeae by immune serum
journal, November 1986
- Rice, P. A.
- Journal of Experimental Medicine, Vol. 164, Issue 5
The Immunomodulatory Activity of Meningococcal Lipoprotein Ag473 Depends on the Conformation Made up of the Lipid and Protein Moieties
journal, July 2012
- Chu, Ching-Liang; Yu, Yen-Ling; Kung, Yueh-Chen
- PLoS ONE, Vol. 7, Issue 7
An Outer Membrane Receptor of Neisseria meningitidis Involved in Zinc Acquisition with Vaccine Potential
journal, July 2010
- Stork, Michiel; Bos, Martine P.; Jongerius, Ilse
- PLoS Pathogens, Vol. 6, Issue 7
Experimental Gonococcal Infection in Male Volunteers: Cumulative Experience with Neisseria gonorrhoeae Strains FA1090 and MS11mkC
journal, January 2011
- Hobbs, Marcia M.; Sparling, P. Frederick; Cohen, Myron S.
- Frontiers in Microbiology, Vol. 2
Solubilization of the Cytoplasmic Membrane of Escherichia coli by the Ionic Detergent Sodium-Lauryl Sarcosinate
journal, September 1973
- Filip, Camille; Fletcher, Gail; Wulff, Judith L.
- Journal of Bacteriology, Vol. 115, Issue 3
Multiple gonococcal pilin antigenic variants are produced during experimental human infections.
text, January 1994
- J., Wright, C.; E., Jerse, A.; S., Seifert, H.
- The University of North Carolina at Chapel Hill University Libraries
Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254
journal, July 2006
- Vipond, Caroline; Suker, Janet; Jones, Christopher
- PROTEOMICS, Vol. 6, Issue 14
Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen
journal, January 2001
- Krogh, Anders; Larsson, Björn; von Heijne, Gunnar
- Journal of Molecular Biology, Vol. 305, Issue 3
Efficacy trial of a parenteral gonococcal pilus vaccine in men
journal, March 1991
- Boslego, J.; Tramont, E.; Chung, R.
- Vaccine, Vol. 9, Issue 3
In-silico Hierarchical Approach for the Identification of Potential Universal Vaccine Candidates (PUVCs) from Neisseria gonorrhoeae
journal, December 2016
- Jain, Ravi; Sonkar, Subash C.; Chaudhry, Uma
- Journal of Theoretical Biology, Vol. 410
Neisseria meningitidis: pathogenesis and immunity
journal, February 2015
- Pizza, Mariagrazia; Rappuoli, Rino
- Current Opinion in Microbiology, Vol. 23
From Chaperones to the Membrane with a BAM!
journal, October 2016
- Plummer, Ashlee M.; Fleming, Karen G.
- Trends in Biochemical Sciences, Vol. 41, Issue 10
Gonococcal transferrin binding protein chimeras induce bactericidal and growth inhibitory antibodies in mice
journal, October 2007
- Price, Gregory A.; Masri, Heather P.; Hollander, Aimee M.
- Vaccine, Vol. 25, Issue 41
Lipoprotein NMB0928 from Neisseria meningitidis serogroup B as a novel vaccine candidate
journal, December 2007
- Delgado, Maité; Yero, Daniel; Niebla, Olivia
- Vaccine, Vol. 25, Issue 50
The PorB porin from commensal Neisseria lactamica induces Th1 and Th2 immune responses to ovalbumin in mice and is a potential immune adjuvant
journal, February 2008
- Liu, Xiuping; Wetzler, Lee M.; Massari, Paola
- Vaccine, Vol. 26, Issue 6
Identification of new meningococcal serogroup B surface antigens through a systematic analysis of neisserial genomes
journal, December 2009
- Pajon, Rolando; Yero, Daniel; Niebla, Olivia
- Vaccine, Vol. 28, Issue 2
Broad vaccine coverage predicted for a bivalent recombinant factor H binding protein based vaccine to prevent serogroup B meningococcal disease
journal, August 2010
- Jiang, Han-Qing; Hoiseth, Susan K.; Harris, Shannon L.
- Vaccine, Vol. 28, Issue 37
The new multicomponent vaccine against meningococcal serogroup B, 4CMenB: Immunological, functional and structural characterization of the antigens
journal, May 2012
- Serruto, Davide; Bottomley, Matthew J.; Ram, Sanjay
- Vaccine, Vol. 30
Gonorrhoea vaccines: a step in the right direction
journal, September 2017
- Seib, Kate L.
- The Lancet, Vol. 390, Issue 10102
Copper(II)-Induced Restructuring of ZnuD, a Zinc(II) Transporter from Neisseria meningitidis
journal, April 2019
- Hecel, Aleksandra; Rowińska-Żyrek, Magdalena; Kozłowski, Henryk
- Inorganic Chemistry, Vol. 58, Issue 9
Experimental vaccine induces Th1-driven immune responses and resistance to Neisseria gonorrhoeae infection in a murine model
journal, March 2017
- Liu, Y.; Hammer, L. A.; Liu, W.
- Mucosal Immunology, Vol. 10, Issue 6
Insights into the regulation of protein abundance from proteomic and transcriptomic analyses
journal, March 2012
- Vogel, Christine; Marcotte, Edward M.
- Nature Reviews Genetics, Vol. 13, Issue 4
SignalP 5.0 improves signal peptide predictions using deep neural networks
journal, February 2019
- Almagro Armenteros, José Juan; Tsirigos, Konstantinos D.; Sønderby, Casper Kaae
- Nature Biotechnology, Vol. 37, Issue 4
Red versus green leaves: transcriptomic comparison of foliar senescence between two Prunus cerasifera genotypes
journal, February 2020
- Vangelisti, Alberto; Guidi, Lucia; Cavallini, Andrea
- Scientific Reports, Vol. 10, Issue 1
The gene bglH present in the bgl operon of Escherichia coli, responsible for uptake and fermentation of beta-glucosides encodes for a carbohydrate-specific outer membrane porin
journal, January 1999
- Andersen, Christian; Rak, Bodo; Benz, Roland
- Molecular Microbiology, Vol. 31, Issue 2
Testing the '+2 rule' for lipoprotein sorting in the Escherichia coli cell envelope with a new genetic selection
journal, November 1999
- Seydel, Anke; Gounon, Pierre; Pugsley, Anthony P.
- Molecular Microbiology, Vol. 34, Issue 4
A universal vaccine for serogroup B meningococcus
journal, July 2006
- Giuliani, M. M.; Adu-Bobie, J.; Comanducci, M.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 29
Broad spectrum O-linked protein glycosylation in the human pathogen Neisseria gonorrhoeae
journal, February 2009
- Vik, A.; Aas, F. E.; Anonsen, J. H.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 11
Proteomics-driven Antigen Discovery for Development of Vaccines Against Gonorrhea
journal, May 2016
- Zielke, Ryszard A.; Wierzbicki, Igor H.; Baarda, Benjamin I.
- Molecular & Cellular Proteomics, Vol. 15, Issue 7
Quantitative Proteomics of the 2016 WHO Neisseria gonorrhoeae Reference Strains Surveys Vaccine Candidates and Antimicrobial Resistance Determinants
journal, October 2018
- El-Rami, Fadi E.; Zielke, Ryszard A.; Wi, Teodora
- Molecular & Cellular Proteomics, Vol. 18, Issue 1
Meningococcal B vaccine (4CMenB): the journey from research to real world experience
journal, December 2018
- Rappuoli, Rino; Pizza, Mariagrazia; Masignani, Vega
- Expert Review of Vaccines, Vol. 17, Issue 12
Gonococcal pilin variants in experimental gonorrhea
journal, May 1987
- Swanson, J.
- Journal of Experimental Medicine, Vol. 165, Issue 5
Molecular Typing of Neisseria gonorrhoeae Causing Repeated Infections: Evolution of Porin during Passage within a Community
journal, February 1999
- Hobbs, Marcia M.; Alcorn, Timothy M.; Davis, Rachael H.
- The Journal of Infectious Diseases, Vol. 179, Issue 2
Intranasal Immunization with Gonococcal Outer Membrane Preparations Reduces the Duration of Vaginal Colonization of Mice by Neisseria gonorrhoeae
journal, September 2000
- Plante, Martin; Jerse, Ann; Hamel, Josée
- The Journal of Infectious Diseases, Vol. 182, Issue 3
PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes
journal, May 2010
- Yu, Nancy Y.; Wagner, James R.; Laird, Matthew R.
- Bioinformatics, Vol. 26, Issue 13
Nasal Inoculation of the Commensal Neisseria lactamica Inhibits Carriage of Neisseria meningitidis by Young Adults: A Controlled Human Infection Study
journal, April 2015
- Deasy, Alice M.; Guccione, Ed; Dale, Adam P.
- Clinical Infectious Diseases, Vol. 60, Issue 10
The ironclad truth: how in vivo transcriptomics and in vitro mechanistic studies shape our understanding of Neisseria gonorrhoeae gene regulation during mucosal infection
journal, May 2017
- Moreau, Matthew R.; Massari, Paola; Genco, Caroline A.
- Pathogens and Disease, Vol. 75, Issue 5
A Meningococcal Native Outer Membrane Vesicle Vaccine With Attenuated Endotoxin and Overexpressed Factor H Binding Protein Elicits Gonococcal Bactericidal Antibodies
journal, October 2018
- Beernink, Peter T.; Ispasanie, Emma; Lewis, Lisa A.
- The Journal of Infectious Diseases, Vol. 219, Issue 7
Updates on the web-based VIOLIN vaccine database and analysis system
journal, November 2013
- He, Yongqun; Racz, Rebecca; Sayers, Samantha
- Nucleic Acids Research, Vol. 42, Issue D1
UniProt: a worldwide hub of protein knowledge
November 2018
- Consortium, The UniProt
- Nucleic Acids Research
The Pfam protein families database in 2019
journal, October 2018
- El-Gebali, Sara; Mistry, Jaina; Bateman, Alex
- Nucleic Acids Research, Vol. 47, Issue D1
Feature-based prediction of non-classical and leaderless protein secretion
journal, May 2004
- Bendtsen, J. D.; Jensen, L. J.; Blom, N.
- Protein Engineering Design and Selection, Vol. 17, Issue 4
A genetic screen reveals a periplasmic copper chaperone required for nitrite reductase activity in pathogenic Neisseria
journal, September 2015
- Jen, Freda E. -C.; Djoko, Karrera Y.; Bent, Stephen J.
- The FASEB Journal, Vol. 29, Issue 9
Future prospects for new vaccines against sexually transmitted infections
journal, January 2016
- Gottlieb, Sami L.; Johnston, Christine
- Current Opinion in Infectious Diseases
Prediction of lipoprotein signal peptides in Gram-negative bacteria
journal, August 2003
- Juncker, Agnieszka S.; Willenbrock, Hanni; von Heijne, Gunnar
- Protein Science, Vol. 12, Issue 8
A genetic screen identifies genes and sites involved in pilin antigenic variation in Neisseria gonorrhoeae : Gonococcal mutants with impaired pilin variation
journal, June 2005
- Sechman, Eric V.; Rohrer, Melissa S.; Seifert, H. Steven
- Molecular Microbiology, Vol. 57, Issue 2
Identification of Vaccine Candidates Against Serogroup B Meningococcus by Whole-Genome Sequencing
journal, March 2000
- Pizza, M.
- Science, Vol. 287, Issue 5459
Both MisR (CpxR) and MisS (CpxA) Are Required for Neisseria gonorrhoeae Infection in a Murine Model of Lower Genital Tract Infection
journal, June 2017
- Gangaiah, Dharanesh; Raterman, Erica L.; Wu, Hong
- Infection and Immunity, Vol. 85, Issue 9
MetQ of Neisseria gonorrhoeae Is a Surface-Expressed Antigen That Elicits Bactericidal and Functional Blocking Antibodies
journal, November 2016
- Semchenko, Evgeny A.; Day, Christopher J.; Seib, Kate L.
- Infection and Immunity, Vol. 85, Issue 2
Heptose I Glycan Substitutions on Neisseria gonorrhoeae Lipooligosaccharide Influence C4b-Binding Protein Binding and Serum Resistance
journal, May 2007
- Ram, S.; Ngampasutadol, J.; Cox, A. D.
- Infection and Immunity, Vol. 75, Issue 8
Novel Protein Substrates of the Phospho-Form Modification System in Neisseria gonorrhoeae and Their Connection to O -Linked Protein Glycosylation
journal, November 2011
- Anonsen, Jan Haug; Egge-Jacobsen, Wolfgang; Aas, Finn Erik
- Infection and Immunity, Vol. 80, Issue 1
Phenotypic Characterization of a copA Mutant of Neisseria gonorrhoeae Identifies a Link between Copper and Nitrosative Stress
journal, December 2011
- Djoko, Karrera Y.; Franiek, Jessica A.; Edwards, Jennifer L.
- Infection and Immunity, Vol. 80, Issue 3
Functional Activities and Epitope Specificity of Human and Murine Antibodies against the Class 4 Outer Membrane Protein (Rmp) of Neisseria meningitidis
journal, March 1999
- Rosenqvist, Einar; Musacchio, Alexis; Aase, Audun
- Infection and Immunity, Vol. 67, Issue 3
Structural and Evolutionary Inference from Molecular Variation in Neisseria Porins
journal, May 1999
- Derrick, Jeremy P.; Urwin, Rachel; Suker, Janet
- Infection and Immunity, Vol. 67, Issue 5
Analysis of the Regulated Transcriptome of Neisseria meningitidis in Human Blood Using a Tiling Array
journal, September 2012
- Del Tordello, E.; Bottini, S.; Muzzi, A.
- Journal of Bacteriology, Vol. 194, Issue 22
Identification of Regulatory Elements That Control Expression of the tbpBA Operon in Neisseria gonorrhoeae
journal, May 2014
- Velez Acevedo, R. N.; Ronpirin, C.; Kandler, J. L.
- Journal of Bacteriology, Vol. 196, Issue 15
Structure of the Recombinant Neisseria gonorrhoeae Adhesin Complex Protein (rNg-ACP) and Generation of Murine Antibodies with Bactericidal Activity against Gonococci
journal, October 2018
- Almonacid-Mendoza, Hannia Liliana; Humbert, MarÃa Victoria; Dijokaite, Aiste
- mSphere, Vol. 3, Issue 5
Intravaginal Administration of Interleukin 12 during Genital Gonococcal Infection in Mice Induces Immunity to Heterologous Strains of Neisseria gonorrhoeae
journal, January 2018
- Liu, Yingru; Perez, Julianny; Hammer, Laura A.
- mSphere, Vol. 3, Issue 1
Transcriptome Analysis of Neisseria gonorrhoeae during Natural Infection Reveals Differential Expression of Antibiotic Resistance Determinants between Men and Women
journal, June 2018
- Nudel, Kathleen; McClure, Ryan; Moreau, Matthew
- mSphere, Vol. 3, Issue 3
Gonorrhoea reinfection in heterosexual STD clinic attendees: longitudinal analysis of risks for first reinfection
journal, April 2003
- Mehta, S. D.
- Sexually Transmitted Infections, Vol. 79, Issue 2
Neisseria gonorrhoeae : Drug Resistance, Mouse Models, and Vaccine Development
journal, September 2017
- Rice, Peter A.; Shafer, William M.; Ram, Sanjay
- Annual Review of Microbiology, Vol. 71, Issue 1
Epidemiologic evidence for the development of serovar-specific immunity after gonococcal infection.
journal, May 1989
- Plummer, F. A.; Simonsen, J. N.; Chubb, H.
- Journal of Clinical Investigation, Vol. 83, Issue 5
Multiple gonococcal pilin antigenic variants are produced during experimental human infections.
journal, June 1994
- Seifert, H. S.; Wright, C. J.; Jerse, A. E.
- Journal of Clinical Investigation, Vol. 93, Issue 6
BIGSdb: Scalable analysis of bacterial genome variation at the population level
journal, December 2010
- Jolley, Keith A.; Maiden, Martin CJ
- BMC Bioinformatics, Vol. 11, Issue 1
VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines
journal, January 2007
- Doytchinova, Irini A.; Flower, Darren R.
- BMC Bioinformatics, Vol. 8, Issue 1
Visual gene-network analysis reveals the cancer gene co-expression in human endometrial cancer
journal, January 2014
- Chou, Wei-Chun; Cheng, An-Lin; Brotto, Marco
- BMC Genomics, Vol. 15, Issue 1
Topological analysis of protein co-abundance networks identifies novel host targets important for HCV infection and pathogenesis
journal, January 2012
- McDermott, Jason E.; Diamond, Deborah L.; Corley, Courtney
- BMC Systems Biology, Vol. 6, Issue 1
Antimicrobial susceptibility of Neisseria gonorrhoeaeisolates from symptomatic men attending the Nanjing sexually transmitted diseases clinic (2011–2012): genetic characteristics of isolates with reduced sensitivity to ceftriaxone
journal, November 2014
- Li, Sai; Su, Xiao-Hong; Le, Wen-Jing
- BMC Infectious Diseases, Vol. 14, Issue 1
Regulation of IL-10 and IL-12 production and function in macrophages and dendritic cells
journal, January 2015
- Ma, Xiaojing; Yan, Wenjun; Zheng, Hua
- F1000Research, Vol. 4
The Immunomodulatory Activity of Meningococcal Lipoprotein Ag473 Depends on the Conformation Made up of the Lipid and Protein Moieties
journal, July 2012
- Chu, Ching-Liang; Yu, Yen-Ling; Kung, Yueh-Chen
- PLoS ONE, Vol. 7, Issue 7
The Gonococcal Transcriptome during Infection of the Lower Genital Tract in Women
journal, August 2015
- McClure, Ryan; Nudel, Kathleen; Massari, Paola
- PLOS ONE, Vol. 10, Issue 8
The novel interaction between Neisseria gonorrhoeae TdfJ and human S100A7 allows gonococci to subvert host zinc restriction
journal, August 2019
- Maurakis, Stavros; Keller, Kayla; Maxwell, C. Noel
- PLOS Pathogens, Vol. 15, Issue 8
Recombinant protein meningococcal serogroup B vaccine combined with outer membrane vesicles
journal, May 2011
- Bai, Xilian; Findlow, Jamie; Borrow, Ray
- Expert Opinion on Biological Therapy, Vol. 11, Issue 7
Recombinant bacterial lipoproteins as vaccine candidates
journal, September 2015
- Leng, Chih-Hsiang; Liu, Shih-Jen; Chen, Hsin-Wei
- Expert Review of Vaccines, Vol. 14, Issue 12
Identification of novel immunogenic proteins of Neisseria gonorrhoeae by phage display
text, January 2019
- Connor, Daniel Oliver; Zantow, Jonas; Hust, Michael
- Universität Potsdam
Pathogenesis of Neisseria gonorrhoeae and the Host Defense in Ascending Infections of Human Fallopian Tube
journal, November 2018
- Lenz, Jonathan D.; Dillard, Joseph P.
- Frontiers in Immunology, Vol. 9
Utility of Hybrid Transferrin Binding Protein Antigens for Protection Against Pathogenic Neisseria Species
journal, February 2019
- Fegan, Jamie E.; Calmettes, Charles; Islam, Epshita A.
- Frontiers in Immunology, Vol. 10
Targeting Lipooligosaccharide (LOS) for a Gonococcal Vaccine
journal, February 2019
- Gulati, Sunita; Shaughnessy, Jutamas; Ram, Sanjay
- Frontiers in Immunology, Vol. 10
Estradiol-Treated Female Mice as Surrogate Hosts for Neisseria gonorrhoeae Genital Tract Infections
journal, January 2011
- Jerse, Ann E.; Wu, Hong; Packiam, Mathanraj
- Frontiers in Microbiology, Vol. 2
Experimental Gonococcal Infection in Male Volunteers: Cumulative Experience with Neisseria gonorrhoeae Strains FA1090 and MS11mkC
journal, January 2011
- Hobbs, Marcia M.; Sparling, P. Frederick; Cohen, Myron S.
- Frontiers in Microbiology, Vol. 2
Integrated in silico Analyses of Regulatory and Metabolic Networks of Synechococcus sp. PCC 7002 Reveal Relationships between Gene Centrality and Essentiality
journal, March 2015
- Song, Hyun-Seob; McClure, Ryan; Bernstein, Hans
- Life, Vol. 5, Issue 2