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Title: Characterization of phthalate-degrading bacteria from Asian carp microbiomes and riverine sediments

Abstract

Phthalates are ubiquitous in the environment due to widespread production and distribution. The carcinogenic compounds dimethyl phthalate (DMP), diethyl phthalate (DEP), and dibutyl phthalate (DBP) are non-covalently bonded to plastics; thus prone to dispersal in various environments. Phthalates not only sorb to riverine sediments, but are also taken up by a variety of aquatic organisms. Asian carp species silver (Hypophthalmichthys molitrix) and bighead (Hypophthaltnichthys nobilis) are exposed to phthalates by ingestion and absorption.,The biodegradation of phthalates has been extensively investigated; however, no studies have isolated phthalate degrading bacteria from aquatic species. The purpose of this study was to characterize the growth and biodegradation kinetics of phthalate-degrading bacteria isolated from the gut, gill, and scale microbiomes of Asian carp, and riverine sediments. 16S rRNA sequencing identified similar genera in sediment and H. molitrix gut microbiome inoculated phthalate enrichments. Achromobacter aegrifaciens strain SKTGEO1 and Pseudomonas japonica strain SKEO1 were enriched from sediments; Bacillus subtilis strain SK18, Pseudomonas putida strain SKTG1, and Consortium SK-1 were enriched from Asian carp microbiomes. Each bacteria isolated was shown to eliminate phthalates from experimental systems. This is the first study documenting the biodegradation of phthalates by bacteria isolated from Asian carp gut and H. molitrix scale microbiomes.

Authors:
 [1];  [2];  [1]
  1. Governors State Univ., University Park, IL (United States)
  2. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Subsurface Biogeochemical Research; USDOE
OSTI Identifier:
1574796
Alternate Identifier(s):
OSTI ID: 1702872
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
International Biodeterioration and Biodegradation
Additional Journal Information:
Journal Volume: 143; Journal Issue: C; Journal ID: ISSN 0964-8305
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Asian carp; bacteria; biodegradation; isolation; microbiomes; phthalates

Citation Formats

Kolb, Steven A., O'Loughlin, Edward J., and Gsell, Timothy C. Characterization of phthalate-degrading bacteria from Asian carp microbiomes and riverine sediments. United States: N. p., 2019. Web. doi:10.1016/j.ibiod.2019.104727.
Kolb, Steven A., O'Loughlin, Edward J., & Gsell, Timothy C. Characterization of phthalate-degrading bacteria from Asian carp microbiomes and riverine sediments. United States. https://doi.org/10.1016/j.ibiod.2019.104727
Kolb, Steven A., O'Loughlin, Edward J., and Gsell, Timothy C. Thu . "Characterization of phthalate-degrading bacteria from Asian carp microbiomes and riverine sediments". United States. https://doi.org/10.1016/j.ibiod.2019.104727. https://www.osti.gov/servlets/purl/1574796.
@article{osti_1574796,
title = {Characterization of phthalate-degrading bacteria from Asian carp microbiomes and riverine sediments},
author = {Kolb, Steven A. and O'Loughlin, Edward J. and Gsell, Timothy C.},
abstractNote = {Phthalates are ubiquitous in the environment due to widespread production and distribution. The carcinogenic compounds dimethyl phthalate (DMP), diethyl phthalate (DEP), and dibutyl phthalate (DBP) are non-covalently bonded to plastics; thus prone to dispersal in various environments. Phthalates not only sorb to riverine sediments, but are also taken up by a variety of aquatic organisms. Asian carp species silver (Hypophthalmichthys molitrix) and bighead (Hypophthaltnichthys nobilis) are exposed to phthalates by ingestion and absorption.,The biodegradation of phthalates has been extensively investigated; however, no studies have isolated phthalate degrading bacteria from aquatic species. The purpose of this study was to characterize the growth and biodegradation kinetics of phthalate-degrading bacteria isolated from the gut, gill, and scale microbiomes of Asian carp, and riverine sediments. 16S rRNA sequencing identified similar genera in sediment and H. molitrix gut microbiome inoculated phthalate enrichments. Achromobacter aegrifaciens strain SKTGEO1 and Pseudomonas japonica strain SKEO1 were enriched from sediments; Bacillus subtilis strain SK18, Pseudomonas putida strain SKTG1, and Consortium SK-1 were enriched from Asian carp microbiomes. Each bacteria isolated was shown to eliminate phthalates from experimental systems. This is the first study documenting the biodegradation of phthalates by bacteria isolated from Asian carp gut and H. molitrix scale microbiomes.},
doi = {10.1016/j.ibiod.2019.104727},
journal = {International Biodeterioration and Biodegradation},
number = C,
volume = 143,
place = {United States},
year = {Thu Jul 18 00:00:00 EDT 2019},
month = {Thu Jul 18 00:00:00 EDT 2019}
}

Journal Article:

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Cited by: 8 works
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Figures / Tables:

Fig. 1 Fig. 1: Microbial community composition of sediments, Asian carp gut microbiomes, and phthalateenriched communities characterized at the phylum level. All systems were dominated by the phylum Proteobacteria. Both sediments and Asian carp gut microbiomes were composed of Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, and Verrucomicrobia in lower abundance. Tenericutes displays amore » shift in the H. molitrix microbiome (SC 4 and SC 5).« less

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