Upregulation of peroxide scavenging enzymes and multidrug efflux proteins highlight an active sodium hypochlorite response in Pseudomonas fluorescens biofilms
Abstract
The oxidative biocide sodium hypochlorite is among the most commonly used antimicrobial agents in the control of surface-attached microbial communities (biofilms). Clarifying the genetic response of microorganisms in biofilms to hypochlorite may contribute to improved biofilm control strategies. Here, RNA-seq was used to investigate the differential gene expression response of industrially relevant Pseudomonas fluorescens biofilms to sub-lethal concentrations of sodium hypochlorite. Pseudomonas biofilms responded to hypochlorite exposure with increased transcription of genes encoding peroxide scavenging enzymes (e.g., alkyl hydroperoxide reductase (Ahp) and hydroperoxide resistance protein (Ohr)), oxidative stress repair enzymes (e.g., the periplasmic sulfoxide reductase YedYZ complex), and multidrug efflux (e.g., MexEF pumps). In addition, genes involved in amino acid synthesis and energy metabolism were down-regulated following hypochlorite exposure. This work improves the current understanding of genetic response mechanisms to biocides and contributes to the optimization of biocides and application strategies.
- Authors:
-
- National Energy Technology Lab. (NETL), Pittsburgh, PA (United States); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States); Univ. of Pittsburgh, Pittsburgh, PA (United States). Dept. of Civil and Environmental Engineering
- U.S. Department of Agriculture, Clay Center, NE (United States). Agricultural Research Service, Roman L. Hruska US Meat Animal Research Center
- National Energy Technology Lab. (NETL), Pittsburgh, PA (United States)
- Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States); Univ. of Pittsburgh, Pittsburgh, PA (United States). Dept. of Civil and Environmental Engineering; Univ. of Notre Dame, South Bend, IN (United States). Dept. of Civil & Environmental Engineering & Earth Sciences
- Publication Date:
- Research Org.:
- National Energy Technology Lab. (NETL), Pittsburgh, PA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1573910
- Report Number(s):
- NETL-PUB-22375
Journal ID: ISSN 0892-7014
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biofouling
- Additional Journal Information:
- Journal Volume: 35; Journal Issue: 3; Journal ID: ISSN 0892-7014
- Publisher:
- Taylor & Francis
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; hypochlorite; biofilm; transcriptome; RNA-seq
Citation Formats
Lipus, Daniel, Vikram, Amit, Gulliver, Djuna, and Bibby, Kyle. Upregulation of peroxide scavenging enzymes and multidrug efflux proteins highlight an active sodium hypochlorite response in Pseudomonas fluorescens biofilms. United States: N. p., 2019.
Web. doi:10.1080/08927014.2019.1605357.
Lipus, Daniel, Vikram, Amit, Gulliver, Djuna, & Bibby, Kyle. Upregulation of peroxide scavenging enzymes and multidrug efflux proteins highlight an active sodium hypochlorite response in Pseudomonas fluorescens biofilms. United States. https://doi.org/10.1080/08927014.2019.1605357
Lipus, Daniel, Vikram, Amit, Gulliver, Djuna, and Bibby, Kyle. Wed .
"Upregulation of peroxide scavenging enzymes and multidrug efflux proteins highlight an active sodium hypochlorite response in Pseudomonas fluorescens biofilms". United States. https://doi.org/10.1080/08927014.2019.1605357. https://www.osti.gov/servlets/purl/1573910.
@article{osti_1573910,
title = {Upregulation of peroxide scavenging enzymes and multidrug efflux proteins highlight an active sodium hypochlorite response in Pseudomonas fluorescens biofilms},
author = {Lipus, Daniel and Vikram, Amit and Gulliver, Djuna and Bibby, Kyle},
abstractNote = {The oxidative biocide sodium hypochlorite is among the most commonly used antimicrobial agents in the control of surface-attached microbial communities (biofilms). Clarifying the genetic response of microorganisms in biofilms to hypochlorite may contribute to improved biofilm control strategies. Here, RNA-seq was used to investigate the differential gene expression response of industrially relevant Pseudomonas fluorescens biofilms to sub-lethal concentrations of sodium hypochlorite. Pseudomonas biofilms responded to hypochlorite exposure with increased transcription of genes encoding peroxide scavenging enzymes (e.g., alkyl hydroperoxide reductase (Ahp) and hydroperoxide resistance protein (Ohr)), oxidative stress repair enzymes (e.g., the periplasmic sulfoxide reductase YedYZ complex), and multidrug efflux (e.g., MexEF pumps). In addition, genes involved in amino acid synthesis and energy metabolism were down-regulated following hypochlorite exposure. This work improves the current understanding of genetic response mechanisms to biocides and contributes to the optimization of biocides and application strategies.},
doi = {10.1080/08927014.2019.1605357},
journal = {Biofouling},
number = 3,
volume = 35,
place = {United States},
year = {Wed May 08 00:00:00 EDT 2019},
month = {Wed May 08 00:00:00 EDT 2019}
}
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