Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste
Abstract
Pectin-rich biomasses, such as citrus peel and sugar beet pulp, hold promise as inexpensive feedstocks for microbial fermentations as enzymatic hydrolysis of their component polysaccharides can be accomplished inexpensively to yield high concentrations of fermentable sugars and D-galacturonic acid (D-galUA). In this study, we tackle a number of challenges associated with engineering a microbial strain to convert pectin-rich hydrolysates into commodity and specialty chemicals. First, we engineer D-galUA utilization into yeast, Saccharomyces cerevisiae. Second, we identify that the mechanism of D-galUA uptake into yeast is mediated by hexose transporters and that consumption of D-galUA is inhibited by D-glucose. Third, we enable co-utilization of D-galUA and D-glucose by identifying and expressing a heterologous transporter, GatA, from Aspergillus niger. Last, we demonstrate the use of this transporter for production of the platform chemical, meso-galactaric acid, directly from industrial Navel orange peel waste.
- Authors:
-
[6];
[7]
- Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
- Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
- Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering
- Technical Univ. of Denmark, Lyngby (Denmark). Dept. of Biotechnology and Biomedicine
- Technische Univ. München, Freising (Germany); Max Planck Inst. of Molecular Plant Physiology, Potsdam, (Germany)
- Technische Univ. München, Freising (Germany)
- Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems & Engineering Division
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1571943
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Protzko, Ryan J., Latimer, Luke N., Martinho, Ze, de Reus, Elise, Seibert, Tanja, Benz, J. Philipp, and Dueber, John E. Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste. United States: N. p., 2018.
Web. doi:10.1038/s41467-018-07589-w.
Protzko, Ryan J., Latimer, Luke N., Martinho, Ze, de Reus, Elise, Seibert, Tanja, Benz, J. Philipp, & Dueber, John E. Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste. United States. https://doi.org/10.1038/s41467-018-07589-w
Protzko, Ryan J., Latimer, Luke N., Martinho, Ze, de Reus, Elise, Seibert, Tanja, Benz, J. Philipp, and Dueber, John E. Thu .
"Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste". United States. https://doi.org/10.1038/s41467-018-07589-w. https://www.osti.gov/servlets/purl/1571943.
@article{osti_1571943,
title = {Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste},
author = {Protzko, Ryan J. and Latimer, Luke N. and Martinho, Ze and de Reus, Elise and Seibert, Tanja and Benz, J. Philipp and Dueber, John E.},
abstractNote = {Pectin-rich biomasses, such as citrus peel and sugar beet pulp, hold promise as inexpensive feedstocks for microbial fermentations as enzymatic hydrolysis of their component polysaccharides can be accomplished inexpensively to yield high concentrations of fermentable sugars and D-galacturonic acid (D-galUA). In this study, we tackle a number of challenges associated with engineering a microbial strain to convert pectin-rich hydrolysates into commodity and specialty chemicals. First, we engineer D-galUA utilization into yeast, Saccharomyces cerevisiae. Second, we identify that the mechanism of D-galUA uptake into yeast is mediated by hexose transporters and that consumption of D-galUA is inhibited by D-glucose. Third, we enable co-utilization of D-galUA and D-glucose by identifying and expressing a heterologous transporter, GatA, from Aspergillus niger. Last, we demonstrate the use of this transporter for production of the platform chemical, meso-galactaric acid, directly from industrial Navel orange peel waste.},
doi = {10.1038/s41467-018-07589-w},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {Thu Nov 29 00:00:00 EST 2018},
month = {Thu Nov 29 00:00:00 EST 2018}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 45 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Biorefinery of waste orange peel
journal, February 2010
- Ángel Siles López, José; Li, Qiang; Thompson, Ian P.
- Critical Reviews in Biotechnology, Vol. 30, Issue 1
Genetic Interaction of Aspergillus nidulans galR, xlnR and araR in Regulating D-Galactose and L-Arabinose Release and Catabolism Gene Expression
journal, November 2015
- Kowalczyk, Joanna E.; Gruben, Birgit S.; Battaglia, Evy
- PLOS ONE, Vol. 10, Issue 11
Functional Survey for Heterologous Sugar Transport Proteins, Using Saccharomyces cerevisiae as a Host
journal, March 2011
- Young, Eric; Poucher, Ashley; Comer, Austin
- Applied and Environmental Microbiology, Vol. 77, Issue 10
Expanding the Scope of Biomass-Derived Chemicals through Tandem Reactions Based on Oxorhenium-Catalyzed Deoxydehydration
journal, November 2013
- Shiramizu, Mika; Toste, F. Dean
- Angewandte Chemie, Vol. 125, Issue 49
d-Galacturonic acid catabolism in microorganisms and its biotechnological relevance
journal, January 2009
- Richard, Peter; Hilditch, Satu
- Applied Microbiology and Biotechnology, Vol. 82, Issue 4
Galactose Transport in Saccharomyces cerevisiae II. Characteristics of Galactose Uptake and Exchange in Galactokinaseless Cells
journal, January 1970
- Kou, Shou-Chang; Christensen, Michael S.; Cirillo, Vincent P.
- Journal of Bacteriology, Vol. 103, Issue 3
Citric acid: emerging applications of key biotechnology industrial product
journal, March 2017
- Ciriminna, Rosaria; Meneguzzo, Francesco; Delisi, Riccardo
- Chemistry Central Journal, Vol. 11, Issue 1
Metabolic engineering applications to renewable resource utilization
journal, April 2000
- Aristidou, Aristos; Penttilä, Merja
- Current Opinion in Biotechnology, Vol. 11, Issue 2
Phylogeny.fr: robust phylogenetic analysis for the non-specialist
journal, May 2008
- Dereeper, A.; Guignon, V.; Blanc, G.
- Nucleic Acids Research, Vol. 36, Issue Web Server
Engineering of yeast hexose transporters to transport D-xylose without inhibition by D-glucose
journal, March 2014
- Farwick, A.; Bruder, S.; Schadeweg, V.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 14
Synthesis of Stereoregular Head , Tail Hydroxylated Nylons Derived from d -Glucose
journal, January 1996
- Chen, Liang; Kiely, Donald E.
- The Journal of Organic Chemistry, Vol. 61, Issue 17
Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase
journal, January 2003
- Agius, Fernanda; González-Lamothe, Rocío; Caballero, José L.
- Nature Biotechnology, Vol. 21, Issue 2
Genome-wide analysis of pectate-induced gene expression in Botrytis cinerea : Identification and functional analysis of putative d -galacturonate transporters
journal, November 2014
- Zhang, Lisha; Hua, Chenlei; Stassen, Joost H. M.
- Fungal Genetics and Biology, Vol. 72
Biotransformation of glycerol into 1,3-propanediol
journal, September 2008
- Willke, Thomas; Vorlop, Klaus
- European Journal of Lipid Science and Technology, Vol. 110, Issue 9
Production of Glucaric Acid from a Synthetic Pathway in Recombinant Escherichia coli
journal, July 2009
- Moon, Tae Seok; Yoon, Sang-Hwal; Lanza, Amanda M.
- Applied and Environmental Microbiology, Vol. 75, Issue 13
New search for pectolytic yeasts
journal, November 1994
- Biely, P.; Sláviková, E.
- Folia Microbiologica, Vol. 39, Issue 6
Pectin-rich biomass as feedstock for fuel ethanol production
journal, June 2012
- Edwards, Meredith C.; Doran-Peterson, Joy
- Applied Microbiology and Biotechnology, Vol. 95, Issue 3
Engineering NADH metabolism in Saccharomyces cerevisiae : formate as an electron donor for glycerol production by anaerobic, glucose-limited chemostat cultures
journal, December 2006
- Geertman, Jan-Maarten A.; van Dijken, Johannes P.; Pronk, Jack T.
- FEMS Yeast Research, Vol. 6, Issue 8
Roles of pectin in biomass yield and processing for biofuels
journal, January 2013
- Xiao, Chaowen; Anderson, Charles T.
- Frontiers in Plant Science, Vol. 4
One-step Synthesis of Dibutyl Furandicarboxylates from Galactaric Acid
journal, January 2008
- Taguchi, Yoichi; Oishi, Akihiro; Iida, Hiroshi
- Chemistry Letters, Vol. 37, Issue 1
Production of ethanol from enzymatically hydrolyzed orange peel by the yeastSaccharomyces cerevisiae
journal, March 1994
- Grohmann, Karel; Baldwin, Elizabeth A.; Buslig, Béla S.
- Applied Biochemistry and Biotechnology, Vol. 45-46, Issue 1
Production of Glucaric Acid from a Synthetic Pathway in Recombinant Escherichia coli
journal, December 2008
- Moon, T. S.; Yoon, S. -H.; Lanza, A. M.
- Applied and Environmental Microbiology, Vol. 75, Issue 3
Submerged Citric Acid Fermentation on Orange Peel Autohydrolysate
journal, April 2008
- Rivas, Beatriz; Torrado, Ana; Torre, Paolo
- Journal of Agricultural and Food Chemistry, Vol. 56, Issue 7
Engineering Filamentous Fungi for Conversion of d-Galacturonic Acid to l-Galactonic Acid
journal, October 2012
- Kuivanen, Joosu; Mojzita, Dominik; Wang, Yanming
- Applied and Environmental Microbiology, Vol. 78, Issue 24
Expanding the Scope of Biomass-Derived Chemicals through Tandem Reactions Based on Oxorhenium-Catalyzed Deoxydehydration
journal, November 2013
- Shiramizu, Mika; Toste, F. Dean
- Angewandte Chemie International Edition, Vol. 52, Issue 49
Metabolic Engineering of Fungal Strains for Conversion of D-Galacturonate to meso-Galactarate
journal, November 2009
- Mojzita, D.; Wiebe, M.; Hilditch, S.
- Applied and Environmental Microbiology, Vol. 76, Issue 1
Overexpression of the Aspergillus niger GatA transporter leads to preferential use of D-galacturonic acid over D-xylose
journal, August 2014
- Sloothaak, Jasper; Schilders, Mike; Schaap, Peter J.
- AMB Express, Vol. 4, Issue 1
Co-ordination of transition metal ions by galactaric acid: a potentiometric and spectroscopic study
journal, November 2002
- Saladini, Monica; Ferrari, Erika; Menabue, Ledi
- Journal of Inorganic Biochemistry, Vol. 92, Issue 2
The sole introduction of two single-point mutations establishes glycerol utilization in Saccharomyces cerevisiae CEN.PK derivatives
journal, January 2017
- Ho, Ping-Wei; Swinnen, Steve; Duitama, Jorge
- Biotechnology for Biofuels, Vol. 10, Issue 1
Identification and isolation of a putative permease gene in the quinic acid utilization (QUT) gene cluster of Aspergillus nidulans
journal, June 1987
- Whittington, Hayley A.; Grant, Susan; Roberts, Clive F.
- Current Genetics, Vol. 12, Issue 2
Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit
journal, February 2017
- Gupta, Apoorv; Reizman, Irene M. Brockman; Reisch, Christopher R.
- Nature Biotechnology, Vol. 35, Issue 3
Replacing fossil based PET with biobased PEF; process analysis, energy and GHG balance
journal, January 2012
- Eerhart, A. J. J. E.; Faaij, A. P. C.; Patel, M. K.
- Energy & Environmental Science, Vol. 5, Issue 4
Toward pectin fermentation by Saccharomyces cerevisiae: Expression of the first two steps of a bacterial pathway for d-galacturonate metabolism
journal, December 2012
- Huisjes, Eline H.; Luttik, Marijke A. H.; Almering, Marinka J. H.
- Journal of Biotechnology, Vol. 162, Issue 2-3
A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
journal, April 2015
- Lee, Michael E.; DeLoache, William C.; Cervantes, Bernardo
- ACS Synthetic Biology, Vol. 4, Issue 9
The economics of current and future biofuels
journal, May 2009
- Tao, Ling; Aden, Andy
- In Vitro Cellular & Developmental Biology - Plant, Vol. 45, Issue 3
Expanding the Scope of Biomass-Derived Chemicals through Tandem Reactions Based on Oxorhenium-Catalyzed Deoxydehydration
journal, November 2013
- Shiramizu, Mika; Toste, F. Dean
- Angewandte Chemie International Edition, Vol. 52, Issue 49
Beneficial Biofuels--The Food, Energy, and Environment Trilemma
journal, July 2009
- Tilman, D.; Socolow, R.; Foley, J. A.
- Science, Vol. 325, Issue 5938
Evidence for rapid uptake of d -galacturonic acid in the yeast Saccharomyces cerevisiae by a channel-type transport system
journal, June 2012
- Souffriau, Ben; den Abt, Tom; Thevelein, Johan M.
- FEBS Letters, Vol. 586, Issue 16
Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis
journal, March 2016
- Zhang, Hongfang; Li, Xiukai; Su, Xiaoyun
- ChemCatChem, Vol. 8, Issue 8
An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation
journal, November 2008
- Martens-Uzunova, Elena S.; Schaap, Peter J.
- Fungal Genetics and Biology, Vol. 45, Issue 11
Reducing life cycle greenhouse gas emissions of corn ethanol by integrating biomass to produce heat and power at ethanol plants
journal, March 2011
- Kaliyan, Nalladurai; Morey, R. Vance; Tiffany, Douglas G.
- Biomass and Bioenergy, Vol. 35, Issue 3
MOESM1 of Engineering Aspergillus niger for galactaric acid production: elimination of galactaric acid catabolism by using RNA sequencing and CRISPR/Cas9
dataset, January 2016
- Kuivanen, Joosu; Wang, Y. -M.; Richard, Peter
- Figshare
New microbial fuels: a biotech perspective
journal, June 2009
- Rude, Mathew A.; Schirmer, Andreas
- Current Opinion in Microbiology, Vol. 12, Issue 3
Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein
journal, April 1999
- Kruckeberg, Arthur L.; Ye, Ling; Berden, Jan A.
- Biochemical Journal, Vol. 339, Issue 2
Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform
journal, December 2013
- Enquist-Newman, Maria; Faust, Ann Marie E.; Bravo, Daniel D.
- Nature, Vol. 505, Issue 7482
Pectin structure and biosynthesis
journal, June 2008
- Mohnen, D.
- Current Opinion in Plant Biology, Vol. 11, Issue 3
Roles of pectin in biomass yield and processing for biofuels
journal, January 2013
- Xiao, Chaowen; Anderson, Charles T.
- Frontiers in Plant Science, Vol. 4
The crystal structure of poly(ethylene furanoate)
journal, October 2016
- Mao, Yimin; Kriegel, Robert M.; Bucknall, David G.
- Polymer, Vol. 102
Evolved hexose transporter enhances xylose uptake and glucose/xylose co-utilization in Saccharomyces cerevisiae
journal, January 2016
- Reider Apel, Amanda; Ouellet, Mario; Szmidt-Middleton, Heather
- Scientific Reports, Vol. 6, Issue 1
Production of Glucaric Acid from a Synthetic Pathway in Recombinant Escherichia coli
journal, July 2009
- Moon, Tae Seok; Yoon, Sang-Hwal; Lanza, Amanda M.
- Applied and Environmental Microbiology, Vol. 75, Issue 13
Genome-wide analysis of pectate-induced gene expression in Botrytis cinerea : Identification and functional analysis of putative d -galacturonate transporters
journal, November 2014
- Zhang, Lisha; Hua, Chenlei; Stassen, Joost H. M.
- Fungal Genetics and Biology, Vol. 72
d-Galacturonic acid catabolism in microorganisms and its biotechnological relevance
journal, January 2009
- Richard, Peter; Hilditch, Satu
- Applied Microbiology and Biotechnology, Vol. 82, Issue 4
Synthetic biology for synthetic chemistry
journal, July 2014
- Keasling, Jay
- New Biotechnology, Vol. 31
Engineering of yeast hexose transporters to transport D-xylose without inhibition by D-glucose
journal, March 2014
- Farwick, A.; Bruder, S.; Schadeweg, V.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 14
Synthesis of Stereoregular Head , Tail Hydroxylated Nylons Derived from d -Glucose
journal, January 1996
- Chen, Liang; Kiely, Donald E.
- The Journal of Organic Chemistry, Vol. 61, Issue 17
Submerged Citric Acid Fermentation on Orange Peel Autohydrolysate
journal, April 2008
- Rivas, Beatriz; Torrado, Ana; Torre, Paolo
- Journal of Agricultural and Food Chemistry, Vol. 56, Issue 7
Identification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes
journal, January 2014
- Benz, J.; Protzko, Ryan J.; Andrich, Jonas MS
- Biotechnology for Biofuels, Vol. 7, Issue 1
Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein
journal, April 1999
- Kruckeberg, Arthur L.; Ye, Ling; Berden, Jan A.
- Biochemical Journal, Vol. 339, Issue 2
Metabolic engineering of the fungal D-galacturonate pathway for L-ascorbic acid production
journal, January 2015
- Kuivanen, Joosu; Penttilä, Merja; Richard, Peter
- Microbial Cell Factories, Vol. 14, Issue 1
Production and applications of carbohydrate-derived sugar acids as generic biobased chemicals
journal, July 2015
- Mehtiö, Tuomas; Toivari, Mervi; Wiebe, Marilyn G.
- Critical Reviews in Biotechnology, Vol. 36, Issue 5
New microbial fuels: a biotech perspective
journal, June 2009
- Rude, Mathew A.; Schirmer, Andreas
- Current Opinion in Microbiology, Vol. 12, Issue 3
An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation
journal, November 2008
- Martens-Uzunova, Elena S.; Schaap, Peter J.
- Fungal Genetics and Biology, Vol. 45, Issue 11
Pectin structure and biosynthesis
journal, June 2008
- Mohnen, D.
- Current Opinion in Plant Biology, Vol. 11, Issue 3
Fermentations of Pectin-Rich Biomass with Recombinant Bacteria to Produce Fuel Ethanol
journal, January 2000
- Doran, Joy Bethune; Cripe, Jennifer; Sutton, Misty
- Applied Biochemistry and Biotechnology, Vol. 84-86, Issue 1-9
Controlling Central Carbon Metabolism for Improved Pathway Yields in Saccharomyces cerevisiae
journal, November 2015
- Tan, Sue Zanne; Manchester, Shawn; Prather, Kristala L. J.
- ACS Synthetic Biology, Vol. 5, Issue 2
Phylogeny.fr: robust phylogenetic analysis for the non-specialist
journal, May 2008
- Dereeper, A.; Guignon, V.; Blanc, G.
- Nucleic Acids Research, Vol. 36, Issue Web Server
Engineering Aspergillus niger for galactaric acid production: elimination of galactaric acid catabolism by using RNA sequencing and CRISPR/Cas9
journal, December 2016
- Kuivanen, Joosu; Wang, Y. -M. Jasmin; Richard, Peter
- Microbial Cell Factories, Vol. 15, Issue 1
Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase
journal, January 2003
- Agius, Fernanda; González-Lamothe, Rocío; Caballero, José L.
- Nature Biotechnology, Vol. 21, Issue 2
Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status
journal, October 2006
- van Maris, Antonius J. A.; Abbott, Derek A.; Bellissimi, Eleonora
- Antonie van Leeuwenhoek, Vol. 90, Issue 4
Metabolic engineering of Saccharomyces cerevisiae  for production of carboxylic acids: current status and challenges
journal, December 2009
- Abbott, Derek A.; Zelle, Rintze M.; Pronk, Jack T.
- FEMS Yeast Research, Vol. 9, Issue 8
Synthetic Biology for Synthetic Chemistry
journal, January 2008
- Keasling, Jay D.
- ACS Chemical Biology, Vol. 3, Issue 1
Renewable fuels from biomass: Technical hurdles and economic assessment of biological routes
journal, February 2015
- Klein-Marcuschamer, Daniel; Blanch, Harvey W.
- AIChE Journal, Vol. 61, Issue 9
Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform
journal, December 2013
- Enquist-Newman, Maria; Faust, Ann Marie E.; Bravo, Daniel D.
- Nature, Vol. 505, Issue 7482
Toward pectin fermentation by Saccharomyces cerevisiae: Expression of the first two steps of a bacterial pathway for d-galacturonate metabolism
journal, December 2012
- Huisjes, Eline H.; Luttik, Marijke A. H.; Almering, Marinka J. H.
- Journal of Biotechnology, Vol. 162, Issue 2-3
Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit
journal, February 2017
- Gupta, Apoorv; Reizman, Irene M. Brockman; Reisch, Christopher R.
- Nature Biotechnology, Vol. 35, Issue 3
Renewable fuels from biomass: Technical hurdles and economic assessment of biological routes
journal, February 2015
- Klein-Marcuschamer, Daniel; Blanch, Harvey W.
- AIChE Journal, Vol. 61, Issue 9
Biotransformation of glycerol into 1,3-propanediol
journal, September 2008
- Willke, Thomas; Vorlop, Klaus
- European Journal of Lipid Science and Technology, Vol. 110, Issue 9
MOESM1 of Engineering Aspergillus niger for galactaric acid production: elimination of galactaric acid catabolism by using RNA sequencing and CRISPR/Cas9
dataset, January 2016
- Kuivanen, Joosu; Wang, Y. -M.; Richard, Peter
- Figshare
Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis
journal, March 2016
- Zhang, Hongfang; Li, Xiukai; Su, Xiaoyun
- ChemCatChem, Vol. 8, Issue 8
Techno-economic analysis of ethanol production from lignocellulosic biomass–a comparison of fermentation, thermo catalytic, and chemocatalytic technologies
journal, February 2021
- Devi, Asha; Niazi, Ambreen; Ramteke, Manojkumar
- Bioprocess and Biosystems Engineering, Vol. 44, Issue 6
Evidence for rapid uptake of d -galacturonic acid in the yeast Saccharomyces cerevisiae by a channel-type transport system
journal, June 2012
- Souffriau, Ben; den Abt, Tom; Thevelein, Johan M.
- FEBS Letters, Vol. 586, Issue 16
Production and applications of carbohydrate-derived sugar acids as generic biobased chemicals
journal, July 2015
- Mehtiö, Tuomas; Toivari, Mervi; Wiebe, Marilyn G.
- Critical Reviews in Biotechnology, Vol. 36, Issue 5
Evaluation of the film and adhesive properties of some block copolymer polyhydroxypolyamides from esterified aldaric acids and diamines
journal, January 2000
- Morton, David W.; Kiely, Donald E.
- Journal of Applied Polymer Science, Vol. 77, Issue 14
Yeasts in sustainable bioethanol production: A review
journal, July 2017
- Mohd Azhar, Siti Hajar; Abdulla, Rahmath; Jambo, Siti Azmah
- Biochemistry and Biophysics Reports, Vol. 10
Replacing fossil based PET with biobased PEF; process analysis, energy and GHG balance
journal, January 2012
- Eerhart, A. J. J. E.; Faaij, A. P. C.; Patel, M. K.
- Energy & Environmental Science, Vol. 5, Issue 4
The introduction of the fungal d-galacturonate pathway enables the consumption of d-galacturonic acid by Saccharomyces cerevisiae
journal, August 2016
- Biz, Alessandra; Sugai-Guérios, Maura Harumi; Kuivanen, Joosu
- Microbial Cell Factories, Vol. 15, Issue 1
Categorisation of sugar acid dehydratases in Aspergillus niger
journal, March 2014
- Motter, Francine A.; Kuivanen, Joosu; Keränen, Hanna
- Fungal Genetics and Biology, Vol. 64
One-step Synthesis of Dibutyl Furandicarboxylates from Galactaric Acid
journal, January 2008
- Taguchi, Yoichi; Oishi, Akihiro; Iida, Hiroshi
- Chemistry Letters, Vol. 37, Issue 1
The sole introduction of two single-point mutations establishes glycerol utilization in Saccharomyces cerevisiae CEN.PK derivatives
journal, January 2017
- Ho, Ping-Wei; Swinnen, Steve; Duitama, Jorge
- Biotechnology for Biofuels, Vol. 10, Issue 1
A molecular transporter engineering approach to improving xylose catabolism in Saccharomyces cerevisiae
journal, July 2012
- Young, Eric M.; Comer, Austin D.; Huang, Huashu
- Metabolic Engineering, Vol. 14, Issue 4, p. 401-411
Galactose Transport in Saccharomyces cerevisiae II. Characteristics of Galactose Uptake and Exchange in Galactokinaseless Cells
journal, January 1970
- Kou, Shou-Chang; Christensen, Michael S.; Cirillo, Vincent P.
- Journal of Bacteriology, Vol. 103, Issue 3
Works referencing / citing this record:
Microbial hexuronate catabolism in biotechnology
journal, January 2019
- Kuivanen, Joosu; Biz, Alessandra; Richard, Peter
- AMB Express, Vol. 9, Issue 1
Spotlight on fungal pectin utilization—from phytopathogenicity to molecular recognition and industrial applications
journal, January 2019
- Schmitz, Kevin; Protzko, Ryan; Zhang, Lisha
- Applied Microbiology and Biotechnology, Vol. 103, Issue 6
Genomewide and Enzymatic Analysis Reveals Efficient D-Galacturonic Acid Metabolism in the Basidiomycete Yeast Rhodosporidium toruloides
journal, December 2019
- Protzko, Ryan J.; Hach, Christina A.; Coradetti, Samuel T.
- mSystems, Vol. 4, Issue 6
Genomewide and Enzymatic Analysis Reveals Efficient D-Galacturonic Acid Metabolism in the Basidiomycete Yeast Rhodosporidium toruloides
journal, December 2019
- Protzko, Ryan J.; Hach, Christina A.; Coradetti, Samuel T.
- mSystems, Vol. 4, Issue 6
Modular Engineering of Biomass Degradation Pathways
journal, April 2019
- Chaves, Julie E.; Presley, Gerald N.; Michener, Joshua K.
- Processes, Vol. 7, Issue 4