Imaging effectiveness calculator for non-design microscope samples
Abstract
When attempting to integrate single-molecule fluorescence microscopy with microfabricated devices such as microfluidic channels, fabrication constraints may prevent using traditional coverslips. Instead, the fabricated devices may require imaging through material with a different thickness or index of refraction. Altering either can easily reduce the quality of the image formation (measured by the Strehl ratio) by a factor of 2 or more, reducing the signal-to-noise ratio accordingly. In such cases, successful detection of single-molecule fluorescence may prove difficult or impossible. Furthermore, we provide software to calculate the effect of non-design materials upon the Strehl ratio or ensquared energy and explore the impact of common materials used in microfabrication.
- Authors:
-
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1570285
- Alternate Identifier(s):
- OSTI ID: 1544590
- Report Number(s):
- SAND-2019-11613J
Journal ID: ISSN 1559-128X; APOPAI; 679980
- Grant/Contract Number:
- AC04-94AL85000; NA0003525
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied Optics
- Additional Journal Information:
- Journal Volume: 58; Journal Issue: 22; Journal ID: ISSN 1559-128X
- Publisher:
- Optical Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Anthony, Stephen M., Miller, Philip R., Timlin, Jerilyn A., and Polsky, Ronen. Imaging effectiveness calculator for non-design microscope samples. United States: N. p., 2019.
Web. doi:10.1364/AO.58.006027.
Anthony, Stephen M., Miller, Philip R., Timlin, Jerilyn A., & Polsky, Ronen. Imaging effectiveness calculator for non-design microscope samples. United States. https://doi.org/10.1364/AO.58.006027
Anthony, Stephen M., Miller, Philip R., Timlin, Jerilyn A., and Polsky, Ronen. Fri .
"Imaging effectiveness calculator for non-design microscope samples". United States. https://doi.org/10.1364/AO.58.006027. https://www.osti.gov/servlets/purl/1570285.
@article{osti_1570285,
title = {Imaging effectiveness calculator for non-design microscope samples},
author = {Anthony, Stephen M. and Miller, Philip R. and Timlin, Jerilyn A. and Polsky, Ronen},
abstractNote = {When attempting to integrate single-molecule fluorescence microscopy with microfabricated devices such as microfluidic channels, fabrication constraints may prevent using traditional coverslips. Instead, the fabricated devices may require imaging through material with a different thickness or index of refraction. Altering either can easily reduce the quality of the image formation (measured by the Strehl ratio) by a factor of 2 or more, reducing the signal-to-noise ratio accordingly. In such cases, successful detection of single-molecule fluorescence may prove difficult or impossible. Furthermore, we provide software to calculate the effect of non-design materials upon the Strehl ratio or ensquared energy and explore the impact of common materials used in microfabrication.},
doi = {10.1364/AO.58.006027},
journal = {Applied Optics},
number = 22,
volume = 58,
place = {United States},
year = {Fri Jul 26 00:00:00 EDT 2019},
month = {Fri Jul 26 00:00:00 EDT 2019}
}
Figures / Tables:
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