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Title: 2D Crystal Engineering of Nanosheets Assembled from Helical Peptide Building Blocks

Abstract

The successful integration of 2D nanomaterials into functional devices hinges on developing fabrication methods that afford hierarchical control across length scales of the entire assembly. We demonstrate structural control over a class of crystalline 2D nanosheets assembled from collagen triple helices. By lengthening the triple helix unit through sequential additions of Pro-Hyp-Gly triads, we achieved sub-angstrom tuning over the 2D lattice. These subtle changes influence the overall nanosheet size, which can be adjusted across the mesoscale size regime. The internal structure was observed by cryo-TEM with direct electron detection, which provides realspace high-resolution images, in which individual triple helices comprising the lattice can be clearly discerned. These results establish a general strategy for tuning the structural hierarchy of 2D nanomaterials that employ rigid, cylindrical structural units.

Authors:
ORCiD logo [1];  [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [1];  [2];  [1]; ORCiD logo [1]; ORCiD logo [4]; ORCiD logo [1]
+ Show Author Affiliations
  1. Emory Univ., Atlanta, GA (United States)
  2. Paul Scherrer Inst. (PSI), Villigen (Switzerland)
  3. Argonne National Lab. (ANL), Argonne, IL (United States). X-ray Science Division
  4. Paul Scherrer Inst. (PSI), Villigen (Switzerland); Univ. of Basel (Switzerland). Center for Cellular Imaging and NanoAnalytics; Leiden Univ. (Netherlands)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1569846
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Angewandte Chemie (International Edition)
Additional Journal Information:
Journal Name: Angewandte Chemie (International Edition); Journal Volume: 58; Journal Issue: 38; Journal ID: ISSN 1433-7851
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; biomaterials; collagen-mimetic peptides; nanoarchitectonics; nanosheets; self-assembly

Citation Formats

Merg, Andrea D., Touponse, Gavin, van Genderen, Eric, Zuo, Xiaobing, Bazrafshan, Alisina, Blum, Thorsten, Hughes, Spencer, Salaita, Khalid, Abrahams, Jan Pieter, and Conticello, Vincent P. 2D Crystal Engineering of Nanosheets Assembled from Helical Peptide Building Blocks. United States: N. p., 2019. Web. doi:10.1002/anie.201906214.
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Merg, Andrea D., Touponse, Gavin, van Genderen, Eric, Zuo, Xiaobing, Bazrafshan, Alisina, Blum, Thorsten, Hughes, Spencer, Salaita, Khalid, Abrahams, Jan Pieter, & Conticello, Vincent P. 2D Crystal Engineering of Nanosheets Assembled from Helical Peptide Building Blocks. United States. https://doi.org/10.1002/anie.201906214
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Merg, Andrea D., Touponse, Gavin, van Genderen, Eric, Zuo, Xiaobing, Bazrafshan, Alisina, Blum, Thorsten, Hughes, Spencer, Salaita, Khalid, Abrahams, Jan Pieter, and Conticello, Vincent P. Wed . "2D Crystal Engineering of Nanosheets Assembled from Helical Peptide Building Blocks". United States. https://doi.org/10.1002/anie.201906214. https://www.osti.gov/servlets/purl/1569846.
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@article{osti_1569846,
title = {2D Crystal Engineering of Nanosheets Assembled from Helical Peptide Building Blocks},
author = {Merg, Andrea D. and Touponse, Gavin and van Genderen, Eric and Zuo, Xiaobing and Bazrafshan, Alisina and Blum, Thorsten and Hughes, Spencer and Salaita, Khalid and Abrahams, Jan Pieter and Conticello, Vincent P.},
abstractNote = {The successful integration of 2D nanomaterials into functional devices hinges on developing fabrication methods that afford hierarchical control across length scales of the entire assembly. We demonstrate structural control over a class of crystalline 2D nanosheets assembled from collagen triple helices. By lengthening the triple helix unit through sequential additions of Pro-Hyp-Gly triads, we achieved sub-angstrom tuning over the 2D lattice. These subtle changes influence the overall nanosheet size, which can be adjusted across the mesoscale size regime. The internal structure was observed by cryo-TEM with direct electron detection, which provides realspace high-resolution images, in which individual triple helices comprising the lattice can be clearly discerned. These results establish a general strategy for tuning the structural hierarchy of 2D nanomaterials that employ rigid, cylindrical structural units.},
doi = {10.1002/anie.201906214},
journal = {Angewandte Chemie (International Edition)},
number = 38,
volume = 58,
place = {United States},
year = {Wed Jul 10 00:00:00 EDT 2019},
month = {Wed Jul 10 00:00:00 EDT 2019}
}
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Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1002/anie.201906214
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Cited by: 25 works
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Figures / Tables:

Figure 1 Figure 1: (a) 2D assemblies constructed from rigid, helical peptide building blocks. (b) Amino acid sequence of CMPs comprise positively charged, neutral, and negatively charged triads. Their folding into collagen triple helices gives rise to the triple helix building unit. Antiparallel packing of triple helices into a tetragonal 2D latticemore » is facilitated via alignment of complementary charge interactions between adjacent triple helices. (c) 4S(X) peptide series with varying number, Z, of Pro-Hyp-Gly triads incorporated into the central block (amp = 4S-aminoproline). We hypothesize that incremental changes to CMP length may offer hierarchical control over the assembly parameters.« less
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    Works referencing / citing this record:

    Stabilization of the triple helix in collagen mimicking peptides
    journal, January 2019

    • Kubyshkin, Vladimir
    • Organic & Biomolecular Chemistry, Vol. 17, Issue 35
    • DOI: 10.1039/c9ob01646e
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      Figure 4 (p. 3)figure
      Figure 5 (p. 4)figure
      Nanosheet Engineering (p. 7)figure
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