Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy
Abstract
Biological CO2 fixation is an important technology that can assist in combating climate change. Here, we show an approach called anaerobic, non-photosynthetic mixotrophy can result in net CO2 fixation when using a reduced feedstock. This approach uses microbes called acetogens that are capable of concurrent utilization of both organic and inorganic substrates. In this study, we investigated the substrate utilization of 17 different acetogens, both mesophilic and thermophilic, on a variety of different carbohydrates and gases. Compared to most model acetogen strains, several non-model mesophilic strains displayed greater substrate flexibility, including the ability to utilize disaccharides, glycerol and an oligosaccharide, and growth rates. Three of these non-model strains (Blautia producta, Clostridium scatologenes and Thermoanaerobacter kivui) were chosen for further characterization, under a variety of conditions including H2- or syngas-fed sugar fermentations and a CO2-fed glycerol fermentation. In all cases, CO2 was fixed and carbon yields approached 100%. Finally, the model acetogen C. ljungdahlii was engineered to utilize glucose, a non-preferred sugar, while maintaining mixotrophic behavior. This work demonstrates the flexibility and robustness of anaerobic, non-photosynthetic mixotrophy as a technology to help reduce CO2 emissions.
- Authors:
-
- White Dog Labs, Inc., New Castle, DE (United States)
- CelDezyner, Rehovot (Israel)
- Publication Date:
- Research Org.:
- White Dog Labs, Inc., New Castle, DE (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1569044
- Grant/Contract Number:
- EE0007564
- Resource Type:
- Accepted Manuscript
- Journal Name:
- FEMS Microbiology Letters (Online)
- Additional Journal Information:
- Journal Name: FEMS Microbiology Letters (Online); Journal Volume: 365; Journal Issue: 8; Journal ID: ISSN 1574-6968
- Publisher:
- Federation of European Microbiological Societies
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; acetogen; mixotrophy; CO2 fixation; Wood–Ljungdahl pathway; glucose utilization; syngas
Citation Formats
Maru, Biniam T., Munasinghe, Pradeep C., Gilary, Hadar, Jones, Shawn W., and Tracy, Bryan P. Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy. United States: N. p., 2018.
Web. doi:10.1093/femsle/fny039.
Maru, Biniam T., Munasinghe, Pradeep C., Gilary, Hadar, Jones, Shawn W., & Tracy, Bryan P. Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy. United States. https://doi.org/10.1093/femsle/fny039
Maru, Biniam T., Munasinghe, Pradeep C., Gilary, Hadar, Jones, Shawn W., and Tracy, Bryan P. Fri .
"Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy". United States. https://doi.org/10.1093/femsle/fny039. https://www.osti.gov/servlets/purl/1569044.
@article{osti_1569044,
title = {Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy},
author = {Maru, Biniam T. and Munasinghe, Pradeep C. and Gilary, Hadar and Jones, Shawn W. and Tracy, Bryan P.},
abstractNote = {Biological CO2 fixation is an important technology that can assist in combating climate change. Here, we show an approach called anaerobic, non-photosynthetic mixotrophy can result in net CO2 fixation when using a reduced feedstock. This approach uses microbes called acetogens that are capable of concurrent utilization of both organic and inorganic substrates. In this study, we investigated the substrate utilization of 17 different acetogens, both mesophilic and thermophilic, on a variety of different carbohydrates and gases. Compared to most model acetogen strains, several non-model mesophilic strains displayed greater substrate flexibility, including the ability to utilize disaccharides, glycerol and an oligosaccharide, and growth rates. Three of these non-model strains (Blautia producta, Clostridium scatologenes and Thermoanaerobacter kivui) were chosen for further characterization, under a variety of conditions including H2- or syngas-fed sugar fermentations and a CO2-fed glycerol fermentation. In all cases, CO2 was fixed and carbon yields approached 100%. Finally, the model acetogen C. ljungdahlii was engineered to utilize glucose, a non-preferred sugar, while maintaining mixotrophic behavior. This work demonstrates the flexibility and robustness of anaerobic, non-photosynthetic mixotrophy as a technology to help reduce CO2 emissions.},
doi = {10.1093/femsle/fny039},
journal = {FEMS Microbiology Letters (Online)},
number = 8,
volume = 365,
place = {United States},
year = {Fri Feb 16 00:00:00 EST 2018},
month = {Fri Feb 16 00:00:00 EST 2018}
}
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Works referencing / citing this record:
Carbon dioxide to bio‐fuels by mixed and pure microbial cultures isolated from activated sludge: relative evaluation of CO 2 fixation, biodiesel production, and thermodynamic analysis
journal, August 2019
- Mishra, Somesh; Raghuvanshi, Smita; Gupta, Suresh
- Greenhouse Gases: Science and Technology, Vol. 9, Issue 6