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Title: Bacterial community and arsenic functional genes diversity in arsenic contaminated soils from different geographic locations

Abstract

To understand how soil microbial communities and arsenic (As) functional genes respond to soil arsenic (As) contamination, five soils contaminated with As at different levels were collected from diverse geographic locations, incubated for 54 days under flooded conditions, and examined by both MiSeq sequencing of 16S rRNA gene amplicons and functional gene microarray (GeoChip 4.0). The results showed that both bacterial community structure and As functional gene structure differed among geographical locations. The diversity of As functional genes correlated positively with the diversity of 16S rRNA genes (P< 0.05). Higher diversities of As functional genes and 16S rRNA genes were observed in the soils with higher available As. Soil pH, phosphate-extractable As, and amorphous Fe content were the most important factors in shaping the bacterial community structure and As transformation functional genes. Geographic location was also important in controlling both the bacterial community and As transformation functional potential. These findings provide insights into the variation of As transformation functional genes in soils contaminated with different levels of As at different geographic locations, and the impact of environmental As contamination on the soil bacterial community.

Authors:
 [1];  [2];  [2];  [2];  [2];  [3];  [4]
  1. Sichuan Agricultural Univ., Chengdu (China)
  2. Univ. of Oklahoma, Norman, OK (United States)
  3. Nanjing Agricultural Univ. (China); Rothamsted Research, Hertfordshire (United Kingdom)
  4. Univ. of Oklahoma, Norman, OK (United States); Tsinghua Univ., Beijing (China); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1567072
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 12; Journal Issue: 5; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gu, Yunfu, D. Van Nostrand, Joy, Wu, Liyou, He, Zhili, Qin, Yujia, Zhao, Fang-Jie, and Zhou, Jizhong. Bacterial community and arsenic functional genes diversity in arsenic contaminated soils from different geographic locations. United States: N. p., 2017. Web. doi:10.1371/journal.pone.0176696.
Gu, Yunfu, D. Van Nostrand, Joy, Wu, Liyou, He, Zhili, Qin, Yujia, Zhao, Fang-Jie, & Zhou, Jizhong. Bacterial community and arsenic functional genes diversity in arsenic contaminated soils from different geographic locations. United States. https://doi.org/10.1371/journal.pone.0176696
Gu, Yunfu, D. Van Nostrand, Joy, Wu, Liyou, He, Zhili, Qin, Yujia, Zhao, Fang-Jie, and Zhou, Jizhong. Fri . "Bacterial community and arsenic functional genes diversity in arsenic contaminated soils from different geographic locations". United States. https://doi.org/10.1371/journal.pone.0176696. https://www.osti.gov/servlets/purl/1567072.
@article{osti_1567072,
title = {Bacterial community and arsenic functional genes diversity in arsenic contaminated soils from different geographic locations},
author = {Gu, Yunfu and D. Van Nostrand, Joy and Wu, Liyou and He, Zhili and Qin, Yujia and Zhao, Fang-Jie and Zhou, Jizhong},
abstractNote = {To understand how soil microbial communities and arsenic (As) functional genes respond to soil arsenic (As) contamination, five soils contaminated with As at different levels were collected from diverse geographic locations, incubated for 54 days under flooded conditions, and examined by both MiSeq sequencing of 16S rRNA gene amplicons and functional gene microarray (GeoChip 4.0). The results showed that both bacterial community structure and As functional gene structure differed among geographical locations. The diversity of As functional genes correlated positively with the diversity of 16S rRNA genes (P< 0.05). Higher diversities of As functional genes and 16S rRNA genes were observed in the soils with higher available As. Soil pH, phosphate-extractable As, and amorphous Fe content were the most important factors in shaping the bacterial community structure and As transformation functional genes. Geographic location was also important in controlling both the bacterial community and As transformation functional potential. These findings provide insights into the variation of As transformation functional genes in soils contaminated with different levels of As at different geographic locations, and the impact of environmental As contamination on the soil bacterial community.},
doi = {10.1371/journal.pone.0176696},
journal = {PLoS ONE},
number = 5,
volume = 12,
place = {United States},
year = {Fri May 05 00:00:00 EDT 2017},
month = {Fri May 05 00:00:00 EDT 2017}
}

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Cited by: 41 works
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Figures / Tables:

Table 1 Table 1: Diversity indices based on the 16S rRNA gene sequences from the five geographically distributed soils contaminated with different levels of arsenic. See Fig 1 caption for the soil codes.

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Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.