Self-Repair of Structure and Bioactivity in a Supramolecular Nanostructure
Abstract
Supramolecular nanostructures formed through self-assembly can have energy landscapes, which determine their structures and functions depending on the pathways selected for their synthesis and processing and on the conditions they are exposed to after their initial formation. We report here on the structural damage that occurs in supramolecular peptide amphiphile nanostructures, during freezing in aqueous media, and the self-repair pathways that restore their functions. We found that freezing converts long supramolecular nanofibers into shorter ones, compromising their ability to support cell adhesion, but a single heating and cooling cycle reverses the damage and rescues their bioactivity. Thermal energy in this cycle enables noncovalent interactions to reconfigure the nanostructures into the thermodynamically preferred long nanofibers, a repair process that is impeded by kinetic traps. In addition, we found that nanofibers disrupted during freeze-drying also exhibit the ability to undergo thermal self-repair and recovery of their bioactivity, despite the extra disruption caused by the dehydration step. Following both freezing and freeze-drying, which shorten the 1D nanostructures, their self-repair capacity through thermally driven elongation is inhibited by kinetically trapped states, which contain highly stable noncovalent interactions that are difficult to rearrange. These states decrease the extent of thermal nanostructure repair, an observation wemore »
- Authors:
-
- Northwestern Univ., Evanston, IL (United States)
- Northwestern Univ., Evanston, IL (United States); Northwestern Univ., Chicago, IL (United States)
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Center for Bio-Inspired Energy Science (CBES); Argonne National Lab. (ANL), Argonne, IL (United States); Northwestern Univ., Evanston, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1566549
- Alternate Identifier(s):
- OSTI ID: 1822210; OSTI ID: 1846775
- Grant/Contract Number:
- AC02-06CH11357; SC0000989
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nano Letters
- Additional Journal Information:
- Journal Volume: 18; Journal Issue: 11; Journal ID: ISSN 1530-6984
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; 99 GENERAL AND MISCELLANEOUS; 77 NANOSCIENCE AND NANOTECHNOLOGY; catalysis (homogeneous); solar (photovoltaic); bio-inspired; charge transport; mesostructured materials; materials and chemistry by design; synthesis (novel materials); synthesis (self-assembly); supramolecular nanostructures; self-assembly; self-repair; biomaterials; regenerative medicine; cell−nanostructure interactions; Nanostructures, Nanofibers, Freezing, Peptides and proteins, Viscosity; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Chen, Charlotte H., Palmer, Liam C., and Stupp, Samuel I. Self-Repair of Structure and Bioactivity in a Supramolecular Nanostructure. United States: N. p., 2018.
Web. doi:10.1021/acs.nanolett.8b02709.
Chen, Charlotte H., Palmer, Liam C., & Stupp, Samuel I. Self-Repair of Structure and Bioactivity in a Supramolecular Nanostructure. United States. https://doi.org/10.1021/acs.nanolett.8b02709
Chen, Charlotte H., Palmer, Liam C., and Stupp, Samuel I. Wed .
"Self-Repair of Structure and Bioactivity in a Supramolecular Nanostructure". United States. https://doi.org/10.1021/acs.nanolett.8b02709. https://www.osti.gov/servlets/purl/1566549.
@article{osti_1566549,
title = {Self-Repair of Structure and Bioactivity in a Supramolecular Nanostructure},
author = {Chen, Charlotte H. and Palmer, Liam C. and Stupp, Samuel I.},
abstractNote = {Supramolecular nanostructures formed through self-assembly can have energy landscapes, which determine their structures and functions depending on the pathways selected for their synthesis and processing and on the conditions they are exposed to after their initial formation. We report here on the structural damage that occurs in supramolecular peptide amphiphile nanostructures, during freezing in aqueous media, and the self-repair pathways that restore their functions. We found that freezing converts long supramolecular nanofibers into shorter ones, compromising their ability to support cell adhesion, but a single heating and cooling cycle reverses the damage and rescues their bioactivity. Thermal energy in this cycle enables noncovalent interactions to reconfigure the nanostructures into the thermodynamically preferred long nanofibers, a repair process that is impeded by kinetic traps. In addition, we found that nanofibers disrupted during freeze-drying also exhibit the ability to undergo thermal self-repair and recovery of their bioactivity, despite the extra disruption caused by the dehydration step. Following both freezing and freeze-drying, which shorten the 1D nanostructures, their self-repair capacity through thermally driven elongation is inhibited by kinetically trapped states, which contain highly stable noncovalent interactions that are difficult to rearrange. These states decrease the extent of thermal nanostructure repair, an observation we hypothesize applies to supramolecular systems in general and is mechanistically linked to suppressed molecular exchange dynamics.},
doi = {10.1021/acs.nanolett.8b02709},
journal = {Nano Letters},
number = 11,
volume = 18,
place = {United States},
year = {Wed Oct 31 00:00:00 EDT 2018},
month = {Wed Oct 31 00:00:00 EDT 2018}
}
Web of Science
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