A size-invariant bud-duration timer enables robustness in yeast cell size control
Abstract
Cell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant 'timer' specifying the duration of S/G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis.
- Authors:
-
[1];
[2];
[3];
[3];
- Marine Biological Lab., Woods Hole, MA (United States); Geisel School of Medicine at Dartmouth, Hanover, NH (United States)
- Marine Biological Lab., Woods Hole, MA (United States); Max Planck Inst. for the Physics of Complex Systems, Max Planck Inst. of Molecular Cell Biology and Genetics, Dresden (Germany). Center for Systems Biology Dresden
- Marine Biological Lab., Woods Hole, MA (United States); Univ. of California, San Francisco, CA (United States)
- Marine Biological Lab., Woods Hole, MA (United States); Princeton Univ., NJ (United States)
- Univ. of Lausanne, Lausanne (Switzerland); editor
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1561878
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 12; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Allard, Corey A. H., Decker, Franziska, Weiner, Orion D., Toettcher, Jared E., Graziano, Brian R., and Martin, Sophie G. A size-invariant bud-duration timer enables robustness in yeast cell size control. United States: N. p., 2018.
Web. doi:10.1371/journal.pone.0209301.
Allard, Corey A. H., Decker, Franziska, Weiner, Orion D., Toettcher, Jared E., Graziano, Brian R., & Martin, Sophie G. A size-invariant bud-duration timer enables robustness in yeast cell size control. United States. https://doi.org/10.1371/journal.pone.0209301
Allard, Corey A. H., Decker, Franziska, Weiner, Orion D., Toettcher, Jared E., Graziano, Brian R., and Martin, Sophie G. Fri .
"A size-invariant bud-duration timer enables robustness in yeast cell size control". United States. https://doi.org/10.1371/journal.pone.0209301. https://www.osti.gov/servlets/purl/1561878.
@article{osti_1561878,
title = {A size-invariant bud-duration timer enables robustness in yeast cell size control},
author = {Allard, Corey A. H. and Decker, Franziska and Weiner, Orion D. and Toettcher, Jared E. and Graziano, Brian R. and Martin, Sophie G.},
abstractNote = {Cell populations across nearly all forms of life generally maintain a characteristic cell type-dependent size, but how size control is achieved has been a long-standing question. The G1/S boundary of the cell cycle serves as a major point of size control, and mechanisms operating here restrict passage of cells to Start if they are too small. In contrast, it is less clear how size is regulated post-Start, during S/G2/M. To gain further insight into post-Start size control, we prepared budding yeast that can be reversibly blocked from bud initiation. While blocked, cells continue to grow isotropically, increasing their volume by more than an order of magnitude over unperturbed cells. Upon release from their block, giant mothers reenter the cell cycle and their progeny rapidly return to the original unperturbed size. We found this behavior to be consistent with a size-invariant 'timer' specifying the duration of S/G2/M. These results indicate that yeast use at least two distinct mechanisms at different cell cycle phases to ensure size homeostasis.},
doi = {10.1371/journal.pone.0209301},
journal = {PLoS ONE},
number = 12,
volume = 13,
place = {United States},
year = {Fri Dec 21 00:00:00 EST 2018},
month = {Fri Dec 21 00:00:00 EST 2018}
}
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Fig 1: Control of yeast cell size using optogenetics. (A) Exogenous PhyB (phytochrome B; dark green) is fused to the C-terminus of S. cerevisiae Tom20, anchoring it to the mitochondria outer membrane (orange). PIF (phytochromeinteracting factor; light green) is fused to endogenous Bem1 (blue). Illumination with red light drives amore »
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