Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot Fungus Fomitopsis pinicola

Abstract

ABSTRACTWood-decaying fungi tend to have characteristic substrate ranges that partly define their ecological niche.Fomitopsis pinicolais a brown rot species of Polyporales that is reported on 82 species of softwoods and 42 species of hardwoods. We analyzed the gene expression levels and RNA editing profiles ofF. pinicolafrom submerged cultures with ground wood powder (sampled at 5 days) or solid wood wafers (sampled at 10 and 30 days), using aspen, pine, and spruce substrates (aspen was used only in submerged cultures).Fomitopsis pinicolaexpressed similar sets of wood-degrading enzymes typical of brown rot fungi across all culture conditions and time points. Nevertheless, differential gene expression and RNA editing were observed across all pairwise comparisons of substrates and time points. Genes exhibiting differential expression and RNA editing encode diverse enzymes with known or potential function in brown rot decay, including laccase, benzoquinone reductase, aryl alcohol oxidase, cytochrome P450s, and various glycoside hydrolases. There was no overlap between differentially expressed and differentially edited genes, suggesting that these may provideF. pinicolawith independent mechanisms for responding to different conditions. Comparing transcriptomes from submerged cultures and wood wafers, we found that culture conditions had a greater impact on global expression profiles than substrate wood species. In contrast, the suitesmore » of genes subject to RNA editing were much less affected by culture conditions. These findings highlight the need for standardization of culture conditions in studies of gene expression in wood-decaying fungi.IMPORTANCEAll species of wood-decaying fungi occur on a characteristic range of substrates (host plants), which may be broad or narrow. Understanding the mechanisms that enable fungi to grow on particular substrates is important for both fungal ecology and applied uses of different feedstocks in industrial processes. We grew the wood-decaying polyporeFomitopsis pinicolaon three different wood species, aspen, pine, and spruce, under various culture conditions. We examined both gene expression (transcription levels) and RNA editing (posttranscriptional modification of RNA, which can potentially yield different proteins from the same gene). We found thatF. pinicolais able to modify both gene expression and RNA editing profiles across different substrate species and culture conditions. Many of the genes involved encode enzymes with known or predicted functions in wood decay. This work provides clues to how wood-decaying fungi may adjust their arsenal of decay enzymes to accommodate different host substrates.« less

Authors:
 [1];  [2];  [3];  [3];  [4];  [5];  [6];  [3];  [3];  [4];  [5];  [3];  [2];  [1];  [7]
+ Show Author Affiliations
  1. Clark Univ., Worcester, MA (United States)
  2. US Dept. of Agriculture (USDA), Madison, WI (United States). Forest Products Lab.
  3. Univ. of Minnesota, St. Paul, MN (United States)
  4. Univ. of Toronto, ON (Canada)
  5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
  6. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  7. Univ. of Tartu (Estonia)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1544269
Alternate Identifier(s):
OSTI ID: 1619104
Grant/Contract Number:  
AC02-05CH11231; IOS-1456777; IOS-1456548; IOS-1456958; DEB-1457721
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 84; Journal Issue: 16; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Basidiomycetes; decay; lignocellulose; RNA editing; transcriptome

Citation Formats

Wu, Baojun, Gaskell, Jill, Held, Benjamin W., Toapanta, Cristina, Vuong, Thu, Ahrendt, Steven, Lipzen, Anna, Zhang, Jiwei, Schilling, Jonathan S., Master, Emma, Grigoriev, Igor V., Blanchette, Robert A., Cullen, Dan, Hibbett, David S., and Kivisaar, Maia. Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot Fungus Fomitopsis pinicola. United States: N. p., 2018. Web. doi:10.1128/AEM.00991-18.
Copy to clipboard
Wu, Baojun, Gaskell, Jill, Held, Benjamin W., Toapanta, Cristina, Vuong, Thu, Ahrendt, Steven, Lipzen, Anna, Zhang, Jiwei, Schilling, Jonathan S., Master, Emma, Grigoriev, Igor V., Blanchette, Robert A., Cullen, Dan, Hibbett, David S., & Kivisaar, Maia. Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot Fungus Fomitopsis pinicola. United States. https://doi.org/10.1128/AEM.00991-18
Copy to clipboard
Wu, Baojun, Gaskell, Jill, Held, Benjamin W., Toapanta, Cristina, Vuong, Thu, Ahrendt, Steven, Lipzen, Anna, Zhang, Jiwei, Schilling, Jonathan S., Master, Emma, Grigoriev, Igor V., Blanchette, Robert A., Cullen, Dan, Hibbett, David S., and Kivisaar, Maia. Fri . "Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot Fungus Fomitopsis pinicola". United States. https://doi.org/10.1128/AEM.00991-18. https://www.osti.gov/servlets/purl/1544269.
Copy to clipboard
@article{osti_1544269,
title = {Substrate-Specific Differential Gene Expression and RNA Editing in the Brown Rot Fungus Fomitopsis pinicola},
author = {Wu, Baojun and Gaskell, Jill and Held, Benjamin W. and Toapanta, Cristina and Vuong, Thu and Ahrendt, Steven and Lipzen, Anna and Zhang, Jiwei and Schilling, Jonathan S. and Master, Emma and Grigoriev, Igor V. and Blanchette, Robert A. and Cullen, Dan and Hibbett, David S. and Kivisaar, Maia},
abstractNote = {ABSTRACTWood-decaying fungi tend to have characteristic substrate ranges that partly define their ecological niche.Fomitopsis pinicolais a brown rot species of Polyporales that is reported on 82 species of softwoods and 42 species of hardwoods. We analyzed the gene expression levels and RNA editing profiles ofF. pinicolafrom submerged cultures with ground wood powder (sampled at 5 days) or solid wood wafers (sampled at 10 and 30 days), using aspen, pine, and spruce substrates (aspen was used only in submerged cultures).Fomitopsis pinicolaexpressed similar sets of wood-degrading enzymes typical of brown rot fungi across all culture conditions and time points. Nevertheless, differential gene expression and RNA editing were observed across all pairwise comparisons of substrates and time points. Genes exhibiting differential expression and RNA editing encode diverse enzymes with known or potential function in brown rot decay, including laccase, benzoquinone reductase, aryl alcohol oxidase, cytochrome P450s, and various glycoside hydrolases. There was no overlap between differentially expressed and differentially edited genes, suggesting that these may provideF. pinicolawith independent mechanisms for responding to different conditions. Comparing transcriptomes from submerged cultures and wood wafers, we found that culture conditions had a greater impact on global expression profiles than substrate wood species. In contrast, the suites of genes subject to RNA editing were much less affected by culture conditions. These findings highlight the need for standardization of culture conditions in studies of gene expression in wood-decaying fungi.IMPORTANCEAll species of wood-decaying fungi occur on a characteristic range of substrates (host plants), which may be broad or narrow. Understanding the mechanisms that enable fungi to grow on particular substrates is important for both fungal ecology and applied uses of different feedstocks in industrial processes. We grew the wood-decaying polyporeFomitopsis pinicolaon three different wood species, aspen, pine, and spruce, under various culture conditions. We examined both gene expression (transcription levels) and RNA editing (posttranscriptional modification of RNA, which can potentially yield different proteins from the same gene). We found thatF. pinicolais able to modify both gene expression and RNA editing profiles across different substrate species and culture conditions. Many of the genes involved encode enzymes with known or predicted functions in wood decay. This work provides clues to how wood-decaying fungi may adjust their arsenal of decay enzymes to accommodate different host substrates.},
doi = {10.1128/AEM.00991-18},
journal = {Applied and Environmental Microbiology},
number = 16,
volume = 84,
place = {United States},
year = {Fri Jun 08 00:00:00 EDT 2018},
month = {Fri Jun 08 00:00:00 EDT 2018}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1128/AEM.00991-18
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 18 works
Citation information provided by
Web of Science

Figures / Tables:

Table 1 Table 1: Thirty most abundant transcripts in submerged culture containing ground pine as sole carbon sourcea
All figures and tables (9 total)
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
journal, January 2016

Evolution of novel wood decay mechanisms in Agaricales revealed by the genome sequences of Fistulina hepatica and Cylindrobasidium torrendii
journal, March 2015

Comparative Transcriptome and Secretome Analysis of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium
journal, April 2010

  • Vanden Wymelenberg, A.; Gaskell, J.; Mozuch, M.
  • Applied and Environmental Microbiology, Vol. 76, Issue 11
  • DOI: 10.1128/AEM.00058-10

Immunogold labeling of an extracellular substance producing hydroxyl radicals in wood degraded by brown-rot fungusTyromyces palustris
journal, February 2000

  • Hirano, Taeko; Enoki, Akio; Tanaka, Hiromi
  • Journal of Wood Science, Vol. 46, Issue 1
  • DOI: 10.1007/BF00779552

The Phanerochaete chrysosporium secretome: Database predictions and initial mass spectrometry peptide identifications in cellulose-grown medium
journal, July 2005

  • Wymelenberg, Amber Vanden; Sabat, Grzegorz; Martinez, Diego
  • Journal of Biotechnology, Vol. 118, Issue 1, p. 17-34
  • DOI: 10.1016/j.jbiotec.2005.03.010

Proteomic and Functional Analysis of the Cellulase System Expressed by Postia placenta during Brown Rot of Solid Wood
journal, September 2011

  • Ryu, Jae San; Shary, Semarjit; Houtman, Carl J.
  • Applied and Environmental Microbiology, Vol. 77, Issue 22
  • DOI: 10.1128/AEM.05496-11

Lignin-modifying enzymes in filamentous basidiomycetes - ecological, functional and phylogenetic review
journal, February 2010

  • Lundell, Taina K.; Mäkelä, Miia R.; Hildén, Kristiina
  • Journal of Basic Microbiology, Vol. 50, Issue 1
  • DOI: 10.1002/jobm.200900338

Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta
journal, September 2016

  • Zhang, Jiwei; Presley, Gerald N.; Hammel, Kenneth E.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 39
  • DOI: 10.1073/pnas.1608454113

Comparison of Wood Decay among Diverse Lignicolous Fungi
journal, March 1997

  • Worrall, James J.; Anagnost, Susan E.; Zabel, Robert A.
  • Mycologia, Vol. 89, Issue 2
  • DOI: 10.2307/3761073

Characterization of a hydroxyl-radical-producing glycoprotein and its presumptive genes from the white-rot basidiomycete Phanerochaete chrysosporium
journal, February 2007

Evidence for cleavage of lignin by a brown rot basidiomycete
journal, July 2008

Gene Expression Patterns of Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium Are Influenced by Wood Substrate Composition during Degradation
journal, May 2016

  • Skyba, Oleksandr; Cullen, Dan; Douglas, Carl J.
  • Applied and Environmental Microbiology, Vol. 82, Issue 14
  • DOI: 10.1128/AEM.00134-16

Degradation of the lignocellulose complex in wood
journal, December 1995

  • Blanchette, Robert A.
  • Canadian Journal of Botany, Vol. 73, Issue S1
  • DOI: 10.1139/b95-350

Transcriptome and Secretome Analyses of the Wood Decay Fungus Wolfiporia cocos Support Alternative Mechanisms of Lignocellulose Conversion
journal, April 2016

  • Gaskell, Jill; Blanchette, Robert A.; Stewart, Philip E.
  • Applied and Environmental Microbiology, Vol. 82, Issue 13
  • DOI: 10.1128/AEM.00639-16

Substrate-specific transcription of the enigmatic GH61 family of the pathogenic white-rot fungus Heterobasidion irregulare during growth on lignocellulose
journal, June 2012

  • Yakovlev, Igor; Vaaje-Kolstad, Gustav; Hietala, Ari M.
  • Applied Microbiology and Biotechnology, Vol. 95, Issue 4
  • DOI: 10.1007/s00253-012-4206-x

Molecular identification and functional characterization of cytochrome P450 monooxygenases from the brown-rot basidiomycete Postia placenta
journal, September 2011

  • Ide, Masamichi; Ichinose, Hirofumi; Wariishi, Hiroyuki
  • Archives of Microbiology, Vol. 194, Issue 4
  • DOI: 10.1007/s00203-011-0753-2

SignalP 4.0: discriminating signal peptides from transmembrane regions
journal, September 2011

  • Petersen, Thomas Nordahl; Brunak, Søren; von Heijne, Gunnar
  • Nature Methods, Vol. 8, Issue 10
  • DOI: 10.1038/nmeth.1701

Significant levels of extracellular reactive oxygen species produced by brown rot basidiomycetes on cellulose
journal, October 2002

Cell wall alterations in loblolly pine wood decayed by the white-rot fungus, Ceriporiopsis subvermispora
journal, March 1997

Analysis of Character Correlations Among Wood Decay Mechanisms, Mating Systems, and Substrate Ranges in Homobasidiomycetes
journal, March 2001

Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks
journal, March 2012

JACUSA: site-specific identification of RNA editing events from replicate sequencing data
journal, January 2017

Characteristics of Gloeophyllum trabeum Alcohol Oxidase, an Extracellular Source of H2O2 in Brown Rot Decay of Wood
journal, July 2007

  • Daniel, G.; Volc, J.; Filonova, L.
  • Applied and Environmental Microbiology, Vol. 73, Issue 19
  • DOI: 10.1128/AEM.00977-07

featureCounts: an efficient general purpose program for assigning sequence reads to genomic features
journal, November 2013

FungiFun2: a comprehensive online resource for systematic analysis of gene lists from fungal species
journal, October 2014

Fungal hydroquinones contribute to brown rot of wood
journal, December 2006

Polyporales Brown Rot Species Fomitopsis pinicola : Enzyme Activity Profiles, Oxalic Acid Production, and Fe 3+ -Reducing Metabolite Secretion
journal, February 2018

  • Shah, Firoz; Mali, Tuulia; Lundell, Taina K.
  • Applied and Environmental Microbiology, Vol. 84, Issue 8
  • DOI: 10.1128/AEM.02662-17

Pathways for Extracellular Fenton Chemistry in the Brown Rot Basidiomycete Gloeophyllum trabeum
journal, June 2001

Proteomic characterization of lignocellulose-degrading enzymes secreted by Phanerochaete carnosa grown on spruce and microcrystalline cellulose
journal, March 2010

A-to-I RNA editing is developmentally regulated and generally adaptive for sexual reproduction in Neurospora crassa
journal, August 2017

  • Liu, Huiquan; Li, Yang; Chen, Daipeng
  • Proceedings of the National Academy of Sciences, Vol. 114, Issue 37
  • DOI: 10.1073/pnas.1702591114

RNA Editing During Sexual Development Occurs in Distantly Related Filamentous Ascomycetes
journal, April 2017

  • Teichert, Ines; Dahlmann, Tim A.; Kück, Ulrich
  • Genome Biology and Evolution, Vol. 9, Issue 4
  • DOI: 10.1093/gbe/evx052

Degradation of cellulose by basidiomycetous fungi
journal, May 2008

Wood-Rotting Fungi of North America
journal, January 1980

The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes
journal, June 2012

Genome-wide A-to-I RNA editing in fungi independent of ADAR enzymes
journal, March 2016

edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
journal, November 2009

Recent progress in the chemistry of wood hemicelluloses
journal, January 1967

Time-Dependent Profiles of Transcripts Encoding Lignocellulose-Modifying Enzymes of the White Rot Fungus Phanerochaete carnosa Grown on Multiple Wood Substrates
journal, December 2011

  • MacDonald, Jacqueline; Master, Emma R.
  • Applied and Environmental Microbiology, Vol. 78, Issue 5
  • DOI: 10.1128/AEM.06511-11

Effect of pH and Oxalate on Hydroquinone-Derived Hydroxyl Radical Formation during Brown Rot Wood Degradation
journal, October 2003

Secretome analysis of Phanerochaete chrysosporium strain CIRM-BRFM41 grown on softwood
journal, July 2008

  • Ravalason, Holy; Jan, Gwénaël; Mollé, Daniel
  • Applied Microbiology and Biotechnology, Vol. 80, Issue 4
  • DOI: 10.1007/s00253-008-1596-x

Relationship Between Production of Hydroxyl Radicals and Degradation of Wood by the Brown-Rot Fungus, Tyromyces palustris
journal, January 1997

Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen
journal, January 2001

  • Krogh, Anders; Larsson, Björn; von Heijne, Gunnar
  • Journal of Molecular Biology, Vol. 305, Issue 3
  • DOI: 10.1006/jmbi.2000.4315

Role of fungal peroxidases in biological ligninolysis
journal, June 2008

Influence of Populus Genotype on Gene Expression by the Wood Decay Fungus Phanerochaete chrysosporium
journal, July 2014

  • Gaskell, Jill; Marty, Amber; Mozuch, Michael
  • Applied and Environmental Microbiology, Vol. 80, Issue 18
  • DOI: 10.1128/AEM.01604-14

Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis
journal, March 2012

  • Fernandez-Fueyo, E.; Ruiz-Duenas, F. J.; Ferreira, P.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 14, p. 5458-5463
  • DOI: 10.1073/pnas.1119912109

Laccase and Its Role in Production of Extracellular Reactive Oxygen Species during Wood Decay by the Brown Rot Basidiomycete Postia placenta
journal, February 2010

  • Wei, D.; Houtman, C. J.; Kapich, A. N.
  • Applied and Environmental Microbiology, Vol. 76, Issue 7
  • DOI: 10.1128/AEM.02929-09

New Insights into the Ligninolytic Capability of a Wood Decay Ascomycete
journal, August 2007

  • Shary, S.; Ralph, S. A.; Hammel, K. E.
  • Applied and Environmental Microbiology, Vol. 73, Issue 20
  • DOI: 10.1128/AEM.01361-07

Influence of fungal decay by different basidiomycetes on the structural integrity of Norway spruce wood
journal, July 2008

  • Brischke, C.; Welzbacher, C. R.; Huckfeldt, T.
  • Holz als Roh- und Werkstoff, Vol. 66, Issue 6
  • DOI: 10.1007/s00107-008-0257-1

Abundant and Selective RNA-Editing Events in the Medicinal Mushroom Ganoderma lucidum
journal, February 2014

Production of 2,5-dimethoxyhydroquinone by the brown-rot fungus Serpula lacrymans to drive extracellular Fenton reaction
journal, May 2004

  • Shimokawa, T.; Nakamura, M.; Hayashi, N.
  • Holzforschung, Vol. 58, Issue 3
  • DOI: 10.1515/HF.2004.047

Enhanced degradation of softwood versus hardwood by the white-rot fungus Pycnoporus coccineus
journal, December 2015

  • Couturier, Marie; Navarro, David; Chevret, Didier
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0407-8

Peculiarities of brown-rot fungi and biochemical Fenton reaction with regard to their potential as a model for bioprocessing biomass
journal, March 2012

  • Arantes, Valdeir; Jellison, Jody; Goodell, Barry
  • Applied Microbiology and Biotechnology, Vol. 94, Issue 2
  • DOI: 10.1007/s00253-012-3954-y

Genome, transcriptome, and secretome analysis of wood decay fungus Postia placenta supports unique mechanisms of lignocellulose conversion
journal, February 2009

  • Martinez, Diego; Challacombe, Jean; Morgenstern, Ingo
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 6
  • DOI: 10.1073/pnas.0809575106

Chemistry and Microscopy of Wood Decay by Some Higher Ascomycetes
journal, January 1989

Wood Decay by Brown-Rot Fungi: Changes in Pore Structure and Cell Wall Volume
journal, January 1991

Transcriptomic Responses of the Softwood-Degrading White-Rot Fungus Phanerochaete carnosa during Growth on Coniferous and Deciduous Wood
journal, March 2011

  • MacDonald, Jacqueline; Doering, Matt; Canam, Thomas
  • Applied and Environmental Microbiology, Vol. 77, Issue 10
  • DOI: 10.1128/AEM.02490-10

Significant Alteration of Gene Expression in Wood Decay Fungi Postia placenta and Phanerochaete chrysosporium by Plant Species
journal, May 2011

  • Vanden Wymelenberg, Amber; Gaskell, Jill; Mozuch, Michael
  • Applied and Environmental Microbiology, Vol. 77, Issue 13
  • DOI: 10.1128/AEM.00508-11

Comparative Genomics of Early-Diverging Mushroom-Forming Fungi Provides Insights into the Origins of Lignocellulose Decay Capabilities
journal, December 2015

  • Nagy, László G.; Riley, Robert; Tritt, Andrew
  • Molecular Biology and Evolution, Vol. 33, Issue 4
  • DOI: 10.1093/molbev/msv337

Analysis of the Phlebiopsis gigantea Genome, Transcriptome and Secretome Provides Insight into Its Pioneer Colonization Strategies of Wood
journal, December 2014

Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungi
journal, June 2014

  • Riley, Robert; Salamov, Asaf A.; Brown, Daren W.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 27
  • DOI: 10.1073/pnas.1400592111

Extracellular oxidative systems of the lignin-degrading Basidiomycete Phanerochaete chrysosporium
journal, February 2007

HISAT: a fast spliced aligner with low memory requirements
journal, March 2015

  • Kim, Daehwan; Langmead, Ben; Salzberg, Steven L.
  • Nature Methods, Vol. 12, Issue 4
  • DOI: 10.1038/nmeth.3317

Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes
journal, January 2013

  • Levasseur, Anthony; Drula, Elodie; Lombard, Vincent
  • Biotechnology for Biofuels, Vol. 6, Issue 1, Article No. 41
  • DOI: 10.1186/1754-6834-6-41

Comparison of wood decay among diverse lignicolous fungi
journal, March 1997

Development and validation of prognostic index based on autophagy-related genes in patient with head and neck squamous cell carcinoma
journal, July 2020

ARS2/MAGL signaling in glioblastoma stem cells promotes self-renewal and M2-like polarization of tumor-associated macrophages
journal, June 2020

Comparative mitochondrial genome analysis reveals intron dynamics and gene rearrangements in two Trametes species
journal, January 2021

Wood-Rotting Fungi of North America
journal, January 1980

Analysis of Character Correlations Among Wood Decay Mechanisms, Mating Systems, and Substrate Ranges in Homobasidiomycetes
journal, March 2001

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
text, January 2016

  • Bozena, Gabryel,; Kwan, Law, Betty Yuen; Angelo, De Milito,
  • The University of North Carolina at Chapel Hill University Libraries
  • DOI: 10.17615/12t6-p037
    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    A-to-I mRNA editing in fungi: occurrence, function, and evolution
    journal, October 2018

    • Bian, Zhuyun; Ni, Yajia; Xu, Jin-Rong
    • Cellular and Molecular Life Sciences, Vol. 76, Issue 2
    • DOI: 10.1007/s00018-018-2936-3

    Evolution of substrate-specific gene expression and RNA editing in brown rot wood-decaying fungi
    journal, February 2019

    FairBase: a comprehensive database of fungal A-to-I RNA editing
    journal, January 2019

    Decomposition of spruce wood and release of volatile organic compounds depend on decay type, fungal interactions and enzyme production patterns
    journal, August 2019

    • Mali, Tuulia; Mäki, Mari; Hellén, Heidi
    • FEMS Microbiology Ecology, Vol. 95, Issue 9
    • DOI: 10.1093/femsec/fiz135

    Dynamics of the Phanerochaete carnosa transcriptome during growth on aspen and spruce
    journal, November 2018

      Sort by:
      [ × clear filter / sort ]

      Figures / Tables found in this record:

      Table 1 (p. 5)table
      FIG 1 (p. 6)figure
      FIG 2 (p. 7)figure
      FIG 3 (p. 8)figure
      Table 2 (p. 9)table
      FIG 4 (p. 10)figure
      FIG 5 (p. 11)figure
      FIG 6 (p. 12)figure
      FIG 7 (p. 12)figure
        [ × clear filter / sort ]
        Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

        Similar Records in DOE PAGES and OSTI.GOV collections: