Identification of Porphyrin-Silica Composite Nanoparticles using Atmospheric Solids Analysis Probe Mass Spectrometry
Abstract
Porphyrins are critical pigments involved in biological energy transduction processes. Their abilities to absorb light, then convert it to energy, have raised the interest of using porphyrin nanoparticles as photosensitizers in photodynamic therapy. A current study showed that self- assembled porphyrin-silica composite nanoparticles can selectively destroy tumor cells, but detection of the cellular uptake of porphyrin-silica composite nanoparticles was limited to imaging microscopy. In this work, we developed a novel method to rapidly identify porphyrin-silica composite nanoparticles using Atmospheric Solids Analysis Probe-Mass Spectrometry (ASAP-MS). ASAP-MS can directly analyze complex mixtures without the need for sample preparation. Porphyrin-silica composite nanoparticles were vaporized using heated nitrogen desolvation gas, and their thermo-profiles were examined to identify distinct mass- to-charge (M/Z) signatures. HeLa cells were incubated in growth media containing the nanoparticles, and after sufficient washing to remove residual nanoparticles, the cell suspension was loaded onto the end of ASAP glass capillary probe. Upon heating, HeLa cells were degraded and porphyrin-silica composite nanoparticles were released. Vaporized nanoparticles were ionized and detected by MS. Furthermore, the cellular uptake of porphyrin-silica composite nanoparticles was identified using this ASAP-MS method.
- Authors:
-
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Univ. of New Mexico, Albuquerque, NM (United States)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1515214
- Report Number(s):
- SAND-2019-4470J
Journal ID: ISSN 2059-8521; applab; 674884
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Accepted Manuscript
- Journal Name:
- MRS Advances
- Additional Journal Information:
- Journal Volume: 4; Journal Issue: 38-39; Journal ID: ISSN 2059-8521
- Publisher:
- Materials Research Society (MRS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Karler, Casey, Parchert, Kylea J., Ricken, James B., Carson, Bryan, Mowry, Curtis D., Fan, Hongyou, and Ye, Dongmei. Identification of Porphyrin-Silica Composite Nanoparticles using Atmospheric Solids Analysis Probe Mass Spectrometry. United States: N. p., 2019.
Web. doi:10.1557/adv.2019.217.
Karler, Casey, Parchert, Kylea J., Ricken, James B., Carson, Bryan, Mowry, Curtis D., Fan, Hongyou, & Ye, Dongmei. Identification of Porphyrin-Silica Composite Nanoparticles using Atmospheric Solids Analysis Probe Mass Spectrometry. United States. https://doi.org/10.1557/adv.2019.217
Karler, Casey, Parchert, Kylea J., Ricken, James B., Carson, Bryan, Mowry, Curtis D., Fan, Hongyou, and Ye, Dongmei. Mon .
"Identification of Porphyrin-Silica Composite Nanoparticles using Atmospheric Solids Analysis Probe Mass Spectrometry". United States. https://doi.org/10.1557/adv.2019.217. https://www.osti.gov/servlets/purl/1515214.
@article{osti_1515214,
title = {Identification of Porphyrin-Silica Composite Nanoparticles using Atmospheric Solids Analysis Probe Mass Spectrometry},
author = {Karler, Casey and Parchert, Kylea J. and Ricken, James B. and Carson, Bryan and Mowry, Curtis D. and Fan, Hongyou and Ye, Dongmei},
abstractNote = {Porphyrins are critical pigments involved in biological energy transduction processes. Their abilities to absorb light, then convert it to energy, have raised the interest of using porphyrin nanoparticles as photosensitizers in photodynamic therapy. A current study showed that self- assembled porphyrin-silica composite nanoparticles can selectively destroy tumor cells, but detection of the cellular uptake of porphyrin-silica composite nanoparticles was limited to imaging microscopy. In this work, we developed a novel method to rapidly identify porphyrin-silica composite nanoparticles using Atmospheric Solids Analysis Probe-Mass Spectrometry (ASAP-MS). ASAP-MS can directly analyze complex mixtures without the need for sample preparation. Porphyrin-silica composite nanoparticles were vaporized using heated nitrogen desolvation gas, and their thermo-profiles were examined to identify distinct mass- to-charge (M/Z) signatures. HeLa cells were incubated in growth media containing the nanoparticles, and after sufficient washing to remove residual nanoparticles, the cell suspension was loaded onto the end of ASAP glass capillary probe. Upon heating, HeLa cells were degraded and porphyrin-silica composite nanoparticles were released. Vaporized nanoparticles were ionized and detected by MS. Furthermore, the cellular uptake of porphyrin-silica composite nanoparticles was identified using this ASAP-MS method.},
doi = {10.1557/adv.2019.217},
journal = {MRS Advances},
number = 38-39,
volume = 4,
place = {United States},
year = {Mon Apr 29 00:00:00 EDT 2019},
month = {Mon Apr 29 00:00:00 EDT 2019}
}
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