The impact of tomato fruits containing multi-walled carbon nanotube residues on human intestinal epithelial cell barrier function and intestinal microbiome composition
Abstract
Carbon nanomaterials (CNMs) can positively regulate seed germination and enhance plant growth. However, clarification of the impact of plant organs containing absorbed CNMs on animal and human health is a critical step of risk assessment for new nano–agro-technology. In this study, we have taken a comprehensive approach to studying the effect tomato fruits derived from plants exposed to multi-walled carbon nanotubes (CNTs) have on gastrointestinal epithelial barrier integrity and their impact on the human commensal intestinal microbiota using an in vitro cell culture and batch human fecal suspension models. The effects of CNTs on selected pure cultures of Salmonella enterica Typhimurium and Lactobacillus acidophilus were also evaluated. This study demonstrated that CNT-containing fruits or the corresponding residual level of pure CNTs (0.001 μg ml–1) was not sufficient to initiate a significant change in transepithelial resistance and on gene expression of the model T-84 human intestinal epithelial cells. However, at 10 μg ml–1 concentration CNTs were able to penetrate the cell membrane and change the gene expression profile of exposed cells. Furthermore, extracts from CNT-containing fruits had minimal to no effect on human intestinal microbiota as revealed by culture-based analysis and 16S rRNA sequencing.
- Authors:
-
[2];
[3];
[4];
[5]
- Univ. of Arkansas at Little Rock, Little Rock, AR (United States); National Center for Toxicological Research, Jefferson, AR (United States)
- National Center for Toxicological Research, Jefferson, AR (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Namik Kemal Univ., Corlu-Tekirdag (Turkey)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Univ. of Arkansas at Little Rock, Little Rock, AR (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1510589
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nanoscale
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 8; Journal ID: ISSN 2040-3364
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Lahiani, Mohamed H., Khare, Sangeeta, Cerniglia, Carl E., Boy, Ramiz, Ivanov, Ilia N., and Khodakovskaya, Mariya. The impact of tomato fruits containing multi-walled carbon nanotube residues on human intestinal epithelial cell barrier function and intestinal microbiome composition. United States: N. p., 2019.
Web. doi:10.1039/C8NR08604D.
Lahiani, Mohamed H., Khare, Sangeeta, Cerniglia, Carl E., Boy, Ramiz, Ivanov, Ilia N., & Khodakovskaya, Mariya. The impact of tomato fruits containing multi-walled carbon nanotube residues on human intestinal epithelial cell barrier function and intestinal microbiome composition. United States. https://doi.org/10.1039/C8NR08604D
Lahiani, Mohamed H., Khare, Sangeeta, Cerniglia, Carl E., Boy, Ramiz, Ivanov, Ilia N., and Khodakovskaya, Mariya. Tue .
"The impact of tomato fruits containing multi-walled carbon nanotube residues on human intestinal epithelial cell barrier function and intestinal microbiome composition". United States. https://doi.org/10.1039/C8NR08604D. https://www.osti.gov/servlets/purl/1510589.
@article{osti_1510589,
title = {The impact of tomato fruits containing multi-walled carbon nanotube residues on human intestinal epithelial cell barrier function and intestinal microbiome composition},
author = {Lahiani, Mohamed H. and Khare, Sangeeta and Cerniglia, Carl E. and Boy, Ramiz and Ivanov, Ilia N. and Khodakovskaya, Mariya},
abstractNote = {Carbon nanomaterials (CNMs) can positively regulate seed germination and enhance plant growth. However, clarification of the impact of plant organs containing absorbed CNMs on animal and human health is a critical step of risk assessment for new nano–agro-technology. In this study, we have taken a comprehensive approach to studying the effect tomato fruits derived from plants exposed to multi-walled carbon nanotubes (CNTs) have on gastrointestinal epithelial barrier integrity and their impact on the human commensal intestinal microbiota using an in vitro cell culture and batch human fecal suspension models. The effects of CNTs on selected pure cultures of Salmonella enterica Typhimurium and Lactobacillus acidophilus were also evaluated. This study demonstrated that CNT-containing fruits or the corresponding residual level of pure CNTs (0.001 μg ml–1) was not sufficient to initiate a significant change in transepithelial resistance and on gene expression of the model T-84 human intestinal epithelial cells. However, at 10 μg ml–1 concentration CNTs were able to penetrate the cell membrane and change the gene expression profile of exposed cells. Furthermore, extracts from CNT-containing fruits had minimal to no effect on human intestinal microbiota as revealed by culture-based analysis and 16S rRNA sequencing.},
doi = {10.1039/C8NR08604D},
journal = {Nanoscale},
number = 8,
volume = 11,
place = {United States},
year = {Tue Jan 22 00:00:00 EST 2019},
month = {Tue Jan 22 00:00:00 EST 2019}
}
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