Imaging cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon
Abstract
The mechanism of cellulose synthesis has been studied by characterizing the motility of cellulose synthase complexes tagged with a fluorescent protein; however, this approach has been used exclusively on the hypocotyl of Arabidopsis thaliana. Here we characterize cellulose synthase motility in the model grass, Brachypodium distachyon. We generated lines in which mEGFP is fused N-terminal to BdCESA3 or BdCESA6 and which grew indistinguishably from the wild type (Bd21-3) and had dense fluorescent puncta at or near the plasma membrane. Measured with a particle tracking algorithm, the average speed of GFP-BdCESA3 particles in the mesocotyl was 164 ± 78 nm min-1 (error gives standard deviation [SD], n = 1451 particles). Mean speed in the root appeared similar. For comparison, average speed in the A. thaliana hypocotyl expressing GFP-AtCESA6 was 184 ± 86 nm min-1 (n = 2755). For B. distachyon, we quantified root diameter and elongation rate in response to inhibitors of cellulose (dichlorobenylnitrile; DCB), microtubules (oryzalin), or actin (latrunculin B). Neither oryzalin nor latrunculin affected the speed of CESA complexes; whereas, DCB reduced average speed by about 50% in B. distachyon and by about 35% in A. thaliana. Evidently, between these species, CESA motility is well conserved.
- Publication Date:
- Research Org.:
- Univ. of Massachusetts, Amherst, MA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division
- OSTI Identifier:
- 1507735
- Grant/Contract Number:
- FG02-03ER15421
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Liu, Derui, Zehfroosh, Nina, Hancock, Brandon L., Hines, Kevin, Fang, Wenjuan, Kilfoil, Maria, Learned-Miller, Erik, Sanguinet, Karen A., Goldner, Lori S., and Baskin, Tobias I. Imaging cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon. United States: N. p., 2017.
Web. doi:10.1038/s41598-017-14988-4.
Liu, Derui, Zehfroosh, Nina, Hancock, Brandon L., Hines, Kevin, Fang, Wenjuan, Kilfoil, Maria, Learned-Miller, Erik, Sanguinet, Karen A., Goldner, Lori S., & Baskin, Tobias I. Imaging cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon. United States. https://doi.org/10.1038/s41598-017-14988-4
Liu, Derui, Zehfroosh, Nina, Hancock, Brandon L., Hines, Kevin, Fang, Wenjuan, Kilfoil, Maria, Learned-Miller, Erik, Sanguinet, Karen A., Goldner, Lori S., and Baskin, Tobias I. Wed .
"Imaging cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon". United States. https://doi.org/10.1038/s41598-017-14988-4. https://www.osti.gov/servlets/purl/1507735.
@article{osti_1507735,
title = {Imaging cellulose synthase motility during primary cell wall synthesis in the grass Brachypodium distachyon},
author = {Liu, Derui and Zehfroosh, Nina and Hancock, Brandon L. and Hines, Kevin and Fang, Wenjuan and Kilfoil, Maria and Learned-Miller, Erik and Sanguinet, Karen A. and Goldner, Lori S. and Baskin, Tobias I.},
abstractNote = {The mechanism of cellulose synthesis has been studied by characterizing the motility of cellulose synthase complexes tagged with a fluorescent protein; however, this approach has been used exclusively on the hypocotyl of Arabidopsis thaliana. Here we characterize cellulose synthase motility in the model grass, Brachypodium distachyon. We generated lines in which mEGFP is fused N-terminal to BdCESA3 or BdCESA6 and which grew indistinguishably from the wild type (Bd21-3) and had dense fluorescent puncta at or near the plasma membrane. Measured with a particle tracking algorithm, the average speed of GFP-BdCESA3 particles in the mesocotyl was 164 ± 78 nm min-1 (error gives standard deviation [SD], n = 1451 particles). Mean speed in the root appeared similar. For comparison, average speed in the A. thaliana hypocotyl expressing GFP-AtCESA6 was 184 ± 86 nm min-1 (n = 2755). For B. distachyon, we quantified root diameter and elongation rate in response to inhibitors of cellulose (dichlorobenylnitrile; DCB), microtubules (oryzalin), or actin (latrunculin B). Neither oryzalin nor latrunculin affected the speed of CESA complexes; whereas, DCB reduced average speed by about 50% in B. distachyon and by about 35% in A. thaliana. Evidently, between these species, CESA motility is well conserved.},
doi = {10.1038/s41598-017-14988-4},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {Wed Nov 08 00:00:00 EST 2017},
month = {Wed Nov 08 00:00:00 EST 2017}
}
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Cited by: 9 works
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Figure 1: B. distachyon transgenic lines expressing GFP-BdCESA proteins. (Top) Representative plants at the heading stage grown in the greenhouse. T1 and T7 indicate independent transgenic lines. For GFP-CESA6, line T6 is shown. Scale bars = 3 cm. (Middle) Growth parameters for plants at the stage shown above. Data aremore »
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Works referencing / citing this record:
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