Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies
Abstract
We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).
- Authors:
-
[3];
[3];
[1]
- Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems; Freie Univ., Berlin (Germany). Inst. of Chemistry and Biochemistry
- Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany). Dept. of Biomolecular Systems
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States); Max Planck Inst. of Colloids and Interfaces, Potsdam (Germany)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); German Research Foundation (DFG); Max Planck Society (Germany)
- OSTI Identifier:
- 1507521
- Grant/Contract Number:
- FG02-93ER20097; FG05-93ER20097; DBI-0421683; IOS-0923992; PF850/1-1
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Organic and Biomolecular Chemistry
- Additional Journal Information:
- Journal Volume: 15; Journal Issue: 47; Journal ID: ISSN 1477-0520
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Dallabernardina, Pietro, Ruprecht, Colin, Smith, Peter J., Hahn, Michael G., Urbanowicz, Breeanna R., and Pfrengle, Fabian. Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies. United States: N. p., 2017.
Web. doi:10.1039/c7ob02605f.
Dallabernardina, Pietro, Ruprecht, Colin, Smith, Peter J., Hahn, Michael G., Urbanowicz, Breeanna R., & Pfrengle, Fabian. Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies. United States. https://doi.org/10.1039/c7ob02605f
Dallabernardina, Pietro, Ruprecht, Colin, Smith, Peter J., Hahn, Michael G., Urbanowicz, Breeanna R., and Pfrengle, Fabian. Tue .
"Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies". United States. https://doi.org/10.1039/c7ob02605f. https://www.osti.gov/servlets/purl/1507521.
@article{osti_1507521,
title = {Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies},
author = {Dallabernardina, Pietro and Ruprecht, Colin and Smith, Peter J. and Hahn, Michael G. and Urbanowicz, Breeanna R. and Pfrengle, Fabian},
abstractNote = {We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).},
doi = {10.1039/c7ob02605f},
journal = {Organic and Biomolecular Chemistry},
number = 47,
volume = 15,
place = {United States},
year = {Tue Nov 21 00:00:00 EST 2017},
month = {Tue Nov 21 00:00:00 EST 2017}
}
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Cited by: 24 works
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Figures / Tables:
Fig. 1: Schematic representation of the hemicellulosic polysaccharide xyloglucan and the monosaccharide building blocks required for the automated assembly of representative oligosaccharide fragments.
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Works referencing / citing this record:
Automated Glycan Assembly of Plant Oligosaccharides and Their Application in Cell‐Wall Biology
journal, January 2019
- Bartetzko, Max P.; Pfrengle, Fabian
- ChemBioChem, Vol. 20, Issue 7
Desirable plant cell wall traits for higher-quality miscanthus lignocellulosic biomass
journal, April 2019
- da Costa, Ricardo M. F.; Pattathil, Sivakumar; Avci, Utku
- Biotechnology for Biofuels, Vol. 12, Issue 1
Analyzing Xyloglucan Endotransglycosylases by Incorporating Synthetic Oligosaccharides into Plant Cell Walls
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- ChemBioChem, Vol. 19, Issue 8
Desirable plant cell wall traits for higher-quality miscanthus lignocellulosic biomass
journal, April 2019
- da Costa, Ricardo M. F.; Pattathil, Sivakumar; Avci, Utku
- Biotechnology for Biofuels, Vol. 12, Issue 1
Figures / Tables found in this record:
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