Zooming in on protons: Neutron structure of protein kinase A trapped in a product complex
Abstract
The question vis-à-vis the chemistry of phosphoryl group transfer catalyzed by protein kinases remains a major challenge. The neutron diffraction structure of the catalytic subunit of cAMP-dependent protein kinase (PKA-C) provides a more complete chemical portrait of key proton interactions at the active site. By using a high-affinity protein kinase substrate (PKS) peptide, we captured the reaction products, dephosphorylated nucleotide [adenosine diphosphate (ADP)] and phosphorylated PKS (pPKS), bound at the active site. In the complex, the phosphoryl group of the peptide is protonated, whereas the carboxyl group of the catalytic Asp166is not. Our structure, including conserved waters, shows how the peptide links the distal parts of the cleft together, creating a network that engages the entire molecule. By comparing slow-exchanging backbone amides to those determined by the NMR analysis of PKA-C with ADP and inhibitor peptide (PKI), we identified exchangeable amides that likely distinguish catalytic and inhibited states.
- Authors:
-
- Univ. of Tennessee, Knoxville, TN (United States). Bredesen Center
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Neutron Scattering Division
- Inst. Laue-Langevin, Grenoble (France). Large Scale Structures Group
- Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Chemistry; Univ. of Minnesota, Minneapolis, MN (United States). Dept. of Biochemistry, Molecular Biology, and Biophysics
- Univ. of California, San Diego, CA (United States). Dept. of Pharmacology, and Dept. of Chemistry and Biochemistry
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1506775
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Science Advances
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 3; Journal ID: ISSN 2375-2548
- Publisher:
- AAAS
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Gerlits, Oksana, Weiss, Kevin L., Blakeley, Matthew P., Veglia, Gianluigi, Taylor, Susan S., and Kovalevsky, Andrey. Zooming in on protons: Neutron structure of protein kinase A trapped in a product complex. United States: N. p., 2019.
Web. doi:10.1126/sciadv.aav0482.
Gerlits, Oksana, Weiss, Kevin L., Blakeley, Matthew P., Veglia, Gianluigi, Taylor, Susan S., & Kovalevsky, Andrey. Zooming in on protons: Neutron structure of protein kinase A trapped in a product complex. United States. https://doi.org/10.1126/sciadv.aav0482
Gerlits, Oksana, Weiss, Kevin L., Blakeley, Matthew P., Veglia, Gianluigi, Taylor, Susan S., and Kovalevsky, Andrey. Wed .
"Zooming in on protons: Neutron structure of protein kinase A trapped in a product complex". United States. https://doi.org/10.1126/sciadv.aav0482. https://www.osti.gov/servlets/purl/1506775.
@article{osti_1506775,
title = {Zooming in on protons: Neutron structure of protein kinase A trapped in a product complex},
author = {Gerlits, Oksana and Weiss, Kevin L. and Blakeley, Matthew P. and Veglia, Gianluigi and Taylor, Susan S. and Kovalevsky, Andrey},
abstractNote = {The question vis-à-vis the chemistry of phosphoryl group transfer catalyzed by protein kinases remains a major challenge. The neutron diffraction structure of the catalytic subunit of cAMP-dependent protein kinase (PKA-C) provides a more complete chemical portrait of key proton interactions at the active site. By using a high-affinity protein kinase substrate (PKS) peptide, we captured the reaction products, dephosphorylated nucleotide [adenosine diphosphate (ADP)] and phosphorylated PKS (pPKS), bound at the active site. In the complex, the phosphoryl group of the peptide is protonated, whereas the carboxyl group of the catalytic Asp166is not. Our structure, including conserved waters, shows how the peptide links the distal parts of the cleft together, creating a network that engages the entire molecule. By comparing slow-exchanging backbone amides to those determined by the NMR analysis of PKA-C with ADP and inhibitor peptide (PKI), we identified exchangeable amides that likely distinguish catalytic and inhibited states.},
doi = {10.1126/sciadv.aav0482},
journal = {Science Advances},
number = 3,
volume = 5,
place = {United States},
year = {Wed Mar 20 00:00:00 EDT 2019},
month = {Wed Mar 20 00:00:00 EDT 2019}
}
Web of Science
Figures / Tables:
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Works referencing / citing this record:
A shared vision for macromolecular crystallography over the next five years
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- Förster, Andreas; Schulze-Briese, Clemens
- Structural Dynamics, Vol. 6, Issue 6
A shared vision for macromolecular crystallography over the next five years
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- Förster, Andreas; Schulze-Briese, Clemens
- Structural Dynamics, Vol. 6, Issue 6
Figures / Tables found in this record: