Molecular response of poplar to single and combined ozone and drought

Zhang, Jin; Gao, Feng; Jia, Huixia; Hu, Jianjun; Feng, Zhaozhong

doi:10.1016/j.scitotenv.2018.11.195

Title: Molecular response of poplar to single and combined ozone and drought

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Abstract

High concentration in ground-level ozone (O₃) and water deficit affect forest ecosystems service. Earlier we found intercellular CO₂ concentration and isoprene emission were affected by the combination of O₃ and drought, but the molecular mechanisms controlling these phenotypes are still open questions. In this study, we examined the stomatal conductance (g_s) and transcriptome changes in an O₃-sensitive hybrid poplar exposed to two O₃ levels [charcoal-filtered ambient air (CF) and non-filtered ambient air plus 40 ppb (NF40)] and two water conditions [well-watered (W) and moderate drought (D)]. NF40 reduced the g_s more under D than W. We identified the differentially expressed genes (DEGs) from pairwise comparisons and found the poplar's molecular response to drought was counteracted by elevated O₃. From nine clusters obtained through K-means clustering, 12 core transcription factors were identified. DEGs involved in isoprene biosynthesis and phytohormones signal pathways indicate the molecular response and stomatal closure of poplar under O₃ and/or drought might be through MEP/DOXP and ABA-dependent pathways. In addition, 102 Helitrons capturing DEGs were involved in response to O₃ and/or drought and related with ABA-dependent pathway. This integrated analysis provides multi-dimensional insights to understand the molecular response to the combination of O₃ and drought.

Authors:

^[1]; Gao, Feng ^[2]; Jia, Huixia ^[3];

^[3]; Feng, Zhaozhong ^[2]

Chinese Academy of Sciences (CAS), Beijing (China); Chinese Academy of Forestry, Beijing (China); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Chinese Academy of Sciences (CAS), Beijing (China)
Chinese Academy of Forestry, Beijing (China)

Publication Date:: Thu Nov 15 00:00:00 EST 2018

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1503995

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Science of the Total Environment

Additional Journal Information:: Journal Volume: 655; Journal Issue: C; Journal ID: ISSN 0048-9697

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Climate change; Drought; Ground-level ozone; Poplar; Transcriptome; Helitrons; Protein-protein interaction (PPI) network

Citation Formats


                    Zhang, Jin, Gao, Feng, Jia, Huixia, Hu, Jianjun, and Feng, Zhaozhong. Molecular response of poplar to single and combined ozone and drought.  United States: N. p., 2018. 
Web.  doi:10.1016/j.scitotenv.2018.11.195.

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                    Zhang, Jin, Gao, Feng, Jia, Huixia, Hu, Jianjun, & Feng, Zhaozhong. Molecular response of poplar to single and combined ozone and drought.  United States.  https://doi.org/10.1016/j.scitotenv.2018.11.195

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                    Zhang, Jin, Gao, Feng, Jia, Huixia, Hu, Jianjun, and Feng, Zhaozhong. Thu .  
"Molecular response of poplar to single and combined ozone and drought".  United States.  https://doi.org/10.1016/j.scitotenv.2018.11.195.  https://www.osti.gov/servlets/purl/1503995.

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@article{osti_1503995,

  title        = {Molecular response of poplar to single and combined ozone and drought},

  author       = {Zhang, Jin and Gao, Feng and Jia, Huixia and Hu, Jianjun and Feng, Zhaozhong},

  abstractNote = {High concentration in ground-level ozone (O3) and water deficit affect forest ecosystems service. Earlier we found intercellular CO2 concentration and isoprene emission were affected by the combination of O3 and drought, but the molecular mechanisms controlling these phenotypes are still open questions. In this study, we examined the stomatal conductance (gs) and transcriptome changes in an O3-sensitive hybrid poplar exposed to two O3 levels [charcoal-filtered ambient air (CF) and non-filtered ambient air plus 40 ppb (NF40)] and two water conditions [well-watered (W) and moderate drought (D)]. NF40 reduced the gs more under D than W. We identified the differentially expressed genes (DEGs) from pairwise comparisons and found the poplar's molecular response to drought was counteracted by elevated O3. From nine clusters obtained through K-means clustering, 12 core transcription factors were identified. DEGs involved in isoprene biosynthesis and phytohormones signal pathways indicate the molecular response and stomatal closure of poplar under O3 and/or drought might be through MEP/DOXP and ABA-dependent pathways. In addition, 102 Helitrons capturing DEGs were involved in response to O3 and/or drought and related with ABA-dependent pathway. This integrated analysis provides multi-dimensional insights to understand the molecular response to the combination of O3 and drought.},

  doi          = {10.1016/j.scitotenv.2018.11.195},

  journal      = {Science of the Total Environment},

  number       = C,

  volume       = 655,

  place        = {United States},

  year         = {Thu Nov 15 00:00:00 EST 2018},

  month        = {Thu Nov 15 00:00:00 EST 2018}

}

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