BioBits™ Explorer: A modular synthetic biology education kit
Abstract
Hands-on demonstrations greatly enhance the teaching of science, technology, engineering, and mathematics (STEM) concepts and foster engagement and exploration in the sciences. While numerous chemistry and physics classroom demonstrations exist, few biology demonstrations are practical and accessible due to the challenges and concerns of growing living cells in classrooms. We introduce BioBits™ Explorer, a synthetic biology educational kit based on shelf-stable, freeze-dried, cell-free (FD-CF) reactions, which are activated by simply adding water. The FD-CF reactions engage the senses of sight, smell, and touch with outputs that produce fluorescence, fragrances, and hydrogels, respectively. We introduce components that can teach tunable protein expression, enzymatic reactions, biomaterial formation, and biosensors using RNA switches, some of which represent original FD-CF outputs that expand the toolbox of cell-free synthetic biology. The BioBits™ Explorer kit enables hands-on demonstrations of cutting-edge science that are inexpensive and easy to use, circumventing many current barriers for implementing exploratory biology experiments in classrooms.
- Authors:
-
[1];
[2];
[3];
[4];
[5];
[6];
[3];
[7];
[8];
[9]
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Harvard Univ., Boston, MA (United States)
- Harvard Univ., Boston, MA (United States); Harvard Univ., Cambridge, MA (United States)
- Northwestern Univ., Evanston, IL (United States)
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- Harvard Univ., Boston, MA (United States)
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Broad Institute of MIT and Harvard, Cambridge, MA (United States)
- Univ. of Toronto, ON (Canada)
- Northwestern Univ., Evanston, IL (United States); Northwestern Univ., Chicago, IL (United States)
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Harvard Univ., Boston, MA (United States); Broad Institute of MIT and Harvard, Cambridge, MA (United States); Harvard-MIT Program in Health Sciences and Technology, Cambridge, MA (United States)
- Publication Date:
- Research Org.:
- Northwestern Univ., Evanston, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1499921
- Grant/Contract Number:
- SC0018249
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Science Advances
- Additional Journal Information:
- Journal Volume: 4; Journal Issue: 8; Journal ID: ISSN 2375-2548
- Publisher:
- AAAS
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Huang, Ally, Nguyen, Peter Q., Stark, Jessica C., Takahashi, Melissa K., Donghia, Nina, Ferrante, Tom, Dy, Aaron J., Hsu, Karen J., Dubner, Rachel S., Pardee, Keith, Jewett, Michael C., and Collins, James J. BioBits™ Explorer: A modular synthetic biology education kit. United States: N. p., 2018.
Web. doi:10.1126/sciadv.aat5105.
Huang, Ally, Nguyen, Peter Q., Stark, Jessica C., Takahashi, Melissa K., Donghia, Nina, Ferrante, Tom, Dy, Aaron J., Hsu, Karen J., Dubner, Rachel S., Pardee, Keith, Jewett, Michael C., & Collins, James J. BioBits™ Explorer: A modular synthetic biology education kit. United States. https://doi.org/10.1126/sciadv.aat5105
Huang, Ally, Nguyen, Peter Q., Stark, Jessica C., Takahashi, Melissa K., Donghia, Nina, Ferrante, Tom, Dy, Aaron J., Hsu, Karen J., Dubner, Rachel S., Pardee, Keith, Jewett, Michael C., and Collins, James J. Wed .
"BioBits™ Explorer: A modular synthetic biology education kit". United States. https://doi.org/10.1126/sciadv.aat5105. https://www.osti.gov/servlets/purl/1499921.
@article{osti_1499921,
title = {BioBits™ Explorer: A modular synthetic biology education kit},
author = {Huang, Ally and Nguyen, Peter Q. and Stark, Jessica C. and Takahashi, Melissa K. and Donghia, Nina and Ferrante, Tom and Dy, Aaron J. and Hsu, Karen J. and Dubner, Rachel S. and Pardee, Keith and Jewett, Michael C. and Collins, James J.},
abstractNote = {Hands-on demonstrations greatly enhance the teaching of science, technology, engineering, and mathematics (STEM) concepts and foster engagement and exploration in the sciences. While numerous chemistry and physics classroom demonstrations exist, few biology demonstrations are practical and accessible due to the challenges and concerns of growing living cells in classrooms. We introduce BioBits™ Explorer, a synthetic biology educational kit based on shelf-stable, freeze-dried, cell-free (FD-CF) reactions, which are activated by simply adding water. The FD-CF reactions engage the senses of sight, smell, and touch with outputs that produce fluorescence, fragrances, and hydrogels, respectively. We introduce components that can teach tunable protein expression, enzymatic reactions, biomaterial formation, and biosensors using RNA switches, some of which represent original FD-CF outputs that expand the toolbox of cell-free synthetic biology. The BioBits™ Explorer kit enables hands-on demonstrations of cutting-edge science that are inexpensive and easy to use, circumventing many current barriers for implementing exploratory biology experiments in classrooms.},
doi = {10.1126/sciadv.aat5105},
journal = {Science Advances},
number = 8,
volume = 4,
place = {United States},
year = {Wed Aug 01 00:00:00 EDT 2018},
month = {Wed Aug 01 00:00:00 EDT 2018}
}
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Figures / Tables:
Fig. 1: BioBits™ kits: Freeze-dried educational kits. (A) FD-CF demonstrations require only the addition of water to the supplied reactions and incubation for 1 to 20 hours at 25° to 37°C for observation and analysis by students. In contrast, traditional biology experiments require substantial time, resources, and specialized equipment. (B)more »
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