Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination

Yin, Yongchao; Yan, Jun; Chen, Gao; Murdoch, Fadime Kara; Pfisterer, Nina; Löffler, Frank E.

doi:10.1021/acs.est.8b05871

Title: Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination

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Abstract

Organohalide-respiring bacteria are key players for the turnover of organohalogens. At sites impacted with chlorinated ethenes, bioremediation promotes reductive dechlorination; however, stoichiometric conversion to environmentally benign ethene is not always achieved. We demonstrate that nitrous oxide (N₂O), a compound commonly present in groundwater, inhibits organohalide respiration. N₂O concentrations in the low micromolar range decreased dechlorination rates and resulted in incomplete dechlorination of tetrachloroethene (PCE) in Geobacter lovleyi strain SZ and of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC) in Dehalococcoides mccartyi strain BAV₁ axenic cultures. Presumably, N₂O interferes with reductive dechlorination by reacting with super-reduced Co(I)–corrinoids of reductive dehalogenases, which is supported by the finding that N₂O did not inhibit corrinoid-independent fumarate-to-succinate reduction in strain SZ. Kinetic analyses revealed a best fit to the noncompetitive Michaelis–Menten inhibition model and determined N₂O inhibitory constants, K_I, for PCE and cDCE dechlorination of 40.8 ± 3.8 and 21.2 ± 3.5 μM in strain SZ and strain BAV₁, respectively. The lowest K_I value of 9.6 ± 0.4 μM was determined for VC to ethene reductive dechlorination in strain BAV₁, suggesting that this crucial dechlorination step for achieving detoxification is most susceptible to N₂O inhibition. Groundwater N₂O concentrations exceeding 100 μM are not uncommon, especially inmore »« less

Authors:

Yin, Yongchao ^[1]; Yan, Jun ^[2]; Chen, Gao ^[3]; Murdoch, Fadime Kara ^[1]; Pfisterer, Nina ^[4];

^[5]

Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology and Center for Environmental Biotechnology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology; Chinese Academy of Sciences (CAS), Beijing (China). Key Lab. of Pollution Ecology and Environmental Engineering, Inst. of Applied Ecology
Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology and Dept. of Civil and Environmental Engineering
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology and Center for Environmental Biotechnology; Bielefeld Univ., Bielefeld (Germany). RNA Biology and Molecular Physics
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology, Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering, and Dept. of Biosystems Engineering and Soil Science; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division

Publication Date:: Tue Dec 18 00:00:00 EST 2018

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1490567

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Environmental Science and Technology

Additional Journal Information:: Journal Volume: 53; Journal Issue: 2; Journal ID: ISSN 0013-936X

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES

Citation Formats


                    Yin, Yongchao, Yan, Jun, Chen, Gao, Murdoch, Fadime Kara, Pfisterer, Nina, and Löffler, Frank E. Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination.  United States: N. p., 2018. 
Web.  doi:10.1021/acs.est.8b05871.

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                    Yin, Yongchao, Yan, Jun, Chen, Gao, Murdoch, Fadime Kara, Pfisterer, Nina, & Löffler, Frank E. Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination.  United States.  https://doi.org/10.1021/acs.est.8b05871

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                    Yin, Yongchao, Yan, Jun, Chen, Gao, Murdoch, Fadime Kara, Pfisterer, Nina, and Löffler, Frank E. Tue .  
"Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination".  United States.  https://doi.org/10.1021/acs.est.8b05871.  https://www.osti.gov/servlets/purl/1490567.

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@article{osti_1490567,

  title        = {Nitrous Oxide Is a Potent Inhibitor of Bacterial Reductive Dechlorination},

  author       = {Yin, Yongchao and Yan, Jun and Chen, Gao and Murdoch, Fadime Kara and Pfisterer, Nina and Löffler, Frank E.},

  abstractNote = {Organohalide-respiring bacteria are key players for the turnover of organohalogens. At sites impacted with chlorinated ethenes, bioremediation promotes reductive dechlorination; however, stoichiometric conversion to environmentally benign ethene is not always achieved. We demonstrate that nitrous oxide (N2O), a compound commonly present in groundwater, inhibits organohalide respiration. N2O concentrations in the low micromolar range decreased dechlorination rates and resulted in incomplete dechlorination of tetrachloroethene (PCE) in Geobacter lovleyi strain SZ and of cis-1,2-dichloroethene (cDCE) and vinyl chloride (VC) in Dehalococcoides mccartyi strain BAV1 axenic cultures. Presumably, N2O interferes with reductive dechlorination by reacting with super-reduced Co(I)–corrinoids of reductive dehalogenases, which is supported by the finding that N2O did not inhibit corrinoid-independent fumarate-to-succinate reduction in strain SZ. Kinetic analyses revealed a best fit to the noncompetitive Michaelis–Menten inhibition model and determined N2O inhibitory constants, KI, for PCE and cDCE dechlorination of 40.8 ± 3.8 and 21.2 ± 3.5 μM in strain SZ and strain BAV1, respectively. The lowest KI value of 9.6 ± 0.4 μM was determined for VC to ethene reductive dechlorination in strain BAV1, suggesting that this crucial dechlorination step for achieving detoxification is most susceptible to N2O inhibition. Groundwater N2O concentrations exceeding 100 μM are not uncommon, especially in watersheds impacted by nitrate runoff from agricultural sources. Thus, dissolved N2O measurements can inform about cDCE and VC stalls at sites impacted with chlorinated ethenes.},

  doi          = {10.1021/acs.est.8b05871},

  journal      = {Environmental Science and Technology},

  number       = 2,

  volume       = 53,

  place        = {United States},

  year         = {Tue Dec 18 00:00:00 EST 2018},

  month        = {Tue Dec 18 00:00:00 EST 2018}

}

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