The helix-to-sheet transition of an HIV-1 fusion peptide derivative changes the mechanical properties of lipid bilayer membranes
Abstract
Here, a short sequence on the gp41 envelope protein of HIV-1 is integral to infection by the virus. Without this sequence, termed the fusion peptide (FP), the virus is far less effective at fusing with the cellular membrane. One of the interesting features of the isolated FP is that it transitions between an α-helical conformation and a β-sheet conformation in lipid bilayer membranes as a function of lipid composition and concentration, and the transition correlates with fusion. To better understand how the conformations of the FP impact lipid bilayer membranes, a variant of the FP that does not strongly promote fusion, termed gp41rk, was studied. Circular dichroism spectroscopy, dynamic light scattering, small-angle neutron scattering (SANS) and neutron spin echo spectroscopy (NSE) were used to relate the conformation of gp41rk to the structure and mechanical properties of lipid bilayer membrane vesicles composed of a 7:3 molar ratio mixture of 1,2-dimyristoyl-sn-glycero-3-phosphocholine and 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol). At a peptide-to-lipid ratio (P/L) of 1/200, it adopts an α-helical conformation, while gp41rk is a β-sheet at a P/L of 1/50 in the unilamellar vesicles. SANS reveals that the lipid bilayer membrane becomes thicker when gp41rk adopts a β-sheet conformation, which indicates that the high-concentration state of themore »
- Authors:
-
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1489597
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biochimica et Biophysica Acta. Biomembranes
- Additional Journal Information:
- Journal Volume: 1861; Journal Issue: 3; Journal ID: ISSN 0005-2736
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; HIV-1 fusion peptide; Neutron spin echo spectroscopy; Bilayer dynamics; Membrane rigidity
Citation Formats
Heller, William T., and Zolnierczuk, Piotr A. The helix-to-sheet transition of an HIV-1 fusion peptide derivative changes the mechanical properties of lipid bilayer membranes. United States: N. p., 2018.
Web. doi:10.1016/j.bbamem.2018.12.004.
Heller, William T., & Zolnierczuk, Piotr A. The helix-to-sheet transition of an HIV-1 fusion peptide derivative changes the mechanical properties of lipid bilayer membranes. United States. https://doi.org/10.1016/j.bbamem.2018.12.004
Heller, William T., and Zolnierczuk, Piotr A. Wed .
"The helix-to-sheet transition of an HIV-1 fusion peptide derivative changes the mechanical properties of lipid bilayer membranes". United States. https://doi.org/10.1016/j.bbamem.2018.12.004. https://www.osti.gov/servlets/purl/1489597.
@article{osti_1489597,
title = {The helix-to-sheet transition of an HIV-1 fusion peptide derivative changes the mechanical properties of lipid bilayer membranes},
author = {Heller, William T. and Zolnierczuk, Piotr A.},
abstractNote = {Here, a short sequence on the gp41 envelope protein of HIV-1 is integral to infection by the virus. Without this sequence, termed the fusion peptide (FP), the virus is far less effective at fusing with the cellular membrane. One of the interesting features of the isolated FP is that it transitions between an α-helical conformation and a β-sheet conformation in lipid bilayer membranes as a function of lipid composition and concentration, and the transition correlates with fusion. To better understand how the conformations of the FP impact lipid bilayer membranes, a variant of the FP that does not strongly promote fusion, termed gp41rk, was studied. Circular dichroism spectroscopy, dynamic light scattering, small-angle neutron scattering (SANS) and neutron spin echo spectroscopy (NSE) were used to relate the conformation of gp41rk to the structure and mechanical properties of lipid bilayer membrane vesicles composed of a 7:3 molar ratio mixture of 1,2-dimyristoyl-sn-glycero-3-phosphocholine and 1,2-dimyristoyl-sn-glycero-3-phospho-(1'-rac-glycerol). At a peptide-to-lipid ratio (P/L) of 1/200, it adopts an α-helical conformation, while gp41rk is a β-sheet at a P/L of 1/50 in the unilamellar vesicles. SANS reveals that the lipid bilayer membrane becomes thicker when gp41rk adopts a β-sheet conformation, which indicates that the high-concentration state of the peptide increases the order of the lipid acyl chains. At the same time, NSE demonstrates that the bilayer becomes more rigid, demonstrating that the β-sheet conformation, which correlates with fusion for the native FP sequence, stiffens the bilayer. The results have implications for the function of the FP.},
doi = {10.1016/j.bbamem.2018.12.004},
journal = {Biochimica et Biophysica Acta. Biomembranes},
number = 3,
volume = 1861,
place = {United States},
year = {Wed Dec 12 00:00:00 EST 2018},
month = {Wed Dec 12 00:00:00 EST 2018}
}
Web of Science
Works referenced in this record:
The Role of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins in Virus Infection
journal, October 1995
- Freed, Eric O.; Martin, Malcolm A.
- Journal of Biological Chemistry, Vol. 270, Issue 41
A syncytia assay for human immunodeficiency virus type I(H IV-I) envelope protein and its use in studying HIV-I mutations
journal, June 1989
- Felser, James M.; Klimkait, Thomas; Silver, Jonathan
- Virology, Vol. 170, Issue 2
Characterization of the fusion domain of the human immunodeficiency virus type 1 envelope glycoprotein gp41.
journal, June 1990
- Freed, E. O.; Myers, D. J.; Risser, R.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 12
Target cell-specific determinants of membrane fusion within the human immunodeficiency virus type 1 gp120 third variable region and gp41 amino terminus.
journal, January 1992
- Bergeron, L.; Sullivan, N.; Sodroski, J.
- Journal of Virology, Vol. 66, Issue 4
A mutation in the human immunodeficiency virus type 1 transmembrane glycoprotein gp41 dominantly interferes with fusion and infectivity.
journal, January 1992
- Freed, E. O.; Delwart, E. L.; Buchschacher, G. L.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 1
Phospholipid interactions of synthetic peptides representing the N-terminus of HIV gp41
journal, August 1990
- Rafalski, M.; Lear, J. D.; DeGrado, W. F.
- Biochemistry, Vol. 29, Issue 34
Interaction of human immunodeficiency virus (HIV-1) fusion peptides with artificial lipid membranes
journal, October 1990
- Slepushkin, V. A.; Melikyan, G. B.; Sidorova, M. S.
- Biochemical and Biophysical Research Communications, Vol. 172, Issue 2
Interaction of the HIV-1 Fusion Peptide with Phospholipid Vesicles: Different Structural Requirements for Fusion and Leakage
journal, March 1994
- Nieva, Jose L.; Nir, Shlomo; Muga, Arturo
- Biochemistry, Vol. 33, Issue 11
Lipid membrane fusion induced by the human immunodeficiency virus type 1 gp41 N-terminal extremity is determined by its orientation in the lipid bilayer.
journal, January 1996
- Martin, I.; Schaal, H.; Scheid, A.
- Journal of virology, Vol. 70, Issue 1
Permeabilization and fusion of uncharged lipid vesicles induced by the HIV-1 fusion peptide adopting an extended conformation: dose and sequence effects
journal, October 1997
- Pereira, F. B.; Goñi, F. M.; Muga, A.
- Biophysical Journal, Vol. 73, Issue 4
The interactions of the N-terminal fusogenic peptide of HIV-1 gp41 with neutral phospholipids
journal, June 1999
- Curtain, C.; Separovic, Frances; Nielsen, Katherine
- European Biophysics Journal, Vol. 28, Issue 5
Membrane interactions of the synthetic N-terminal peptide of HIV-1 gp41 and its structural analogs
journal, April 1999
- Mobley, Patrick W.; Waring, Alan J.; Sherman, Mark A.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1418, Issue 1
Conformational transitions of membrane-bound HIV-1 fusion peptide
journal, August 2002
- Sáez-Cirión, Asier; Nieva, José L.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1564, Issue 1
Structure and orientation study of fusion peptide FP23 of gp41 from HIV-1 alone or inserted into various lipid membrane models (mono-, bi- and multibi-layers) by FT-IR spectroscopies and Brewster angle microscopy
journal, September 2005
- Castano, Sabine; Desbat, Bernard
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1715, Issue 2
Changes in lipid bilayer structure caused by the helix-to-sheet transition of an HIV-1 gp41 fusion peptide derivative
journal, March 2017
- Heller, William T.; Rai, Durgesh K.
- Chemistry and Physics of Lipids, Vol. 203
A strong correlation between fusogenicity and membrane insertion depth of the HIV fusion peptide
journal, August 2009
- Qiang, W.; Sun, Y.; Weliky, D. P.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 36
Structure and orientation study of Ebola fusion peptide inserted in lipid membrane models
journal, January 2014
- Agopian, Audrey; Castano, Sabine
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1838, Issue 1
Membrane-Dependent Conformation, Dynamics, and Lipid Interactions of the Fusion Peptide of the Paramyxovirus PIV5 from Solid-State NMR
journal, February 2013
- Yao, Hongwei; Hong, Mei
- Journal of Molecular Biology, Vol. 425, Issue 3
Peptide-Induced Asymmetric Distribution of Charged Lipids in a Vesicle Bilayer Revealed by Small-Angle Neutron Scattering
journal, August 2011
- Qian, Shuo; Heller, William T.
- The Journal of Physical Chemistry B, Vol. 115, Issue 32
Alamethicin Disrupts the Cholesterol Distribution in Dimyristoyl Phosphatidylcholine–Cholesterol Lipid Bilayers
journal, September 2014
- Qian, Shuo; Rai, Durgesh; Heller, William T.
- The Journal of Physical Chemistry B, Vol. 118, Issue 38
Melittin-induced cholesterol reorganization in lipid bilayer membranes
journal, October 2015
- Qian, Shuo; Heller, William T.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1848, Issue 10
The Interaction of Melittin with Dimyristoyl Phosphatidylcholine-Dimyristoyl Phosphatidylserine Lipid Bilayer Membranes
journal, November 2016
- Rai, Durgesh K.; Qian, Shuo; Heller, William T.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1858, Issue 11
The extended Q -range small-angle neutron scattering diffractometer at the SNS
journal, July 2010
- Zhao, J. K.; Gao, C. Y.; Liu, D.
- Journal of Applied Crystallography, Vol. 43, Issue 5
Mantid—Data analysis and visualization package for neutron scattering and SR experiments
journal, November 2014
- Arnold, O.; Bilheux, J. C.; Borreguero, J. M.
- Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 764
Absolute calibration of small-angle neutron scattering data
journal, February 1987
- Wignall, G. D.; Bates, F. S.
- Journal of Applied Crystallography, Vol. 20, Issue 1
Understanding inelastically scattered neutrons from water on a time-of-flight small-angle neutron scattering (SANS) instrument
journal, February 2014
- Do, Changwoo; Heller, William T.; Stanley, Christopher
- Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 737
ELLSTAT : shape modeling for solution small-angle scattering of proteins and protein complexes with automated statistical characterization
journal, September 2006
- Heller, William T.
- Journal of Applied Crystallography, Vol. 39, Issue 5
ORNL_SAS : software for calculation of small-angle scattering intensities of proteins and protein complexes
journal, July 2007
- Tjioe, Elina; Heller, William T.
- Journal of Applied Crystallography, Vol. 40, Issue 4
The spin-echo spectrometer at the Spallation Neutron Source (SNS)
journal, December 2012
- Ohl, M.; Monkenbusch, M.; Arend, N.
- Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 696
DrSPINE: Data reduction for SPIN echo experiments
conference, January 2018
- Zolnierczuk, Piotr A.; Holderer, Olaf; Monkenbusch, Michael
- TO THE EAR AND BACK AGAIN - ADVANCES IN AUDITORY BIOPHYSICS: Proceedings of the 13th Mechanics of Hearing Workshop, AIP Conference Proceedings
Undulations and Dynamic Structure Factor of Membranes
journal, December 1996
- Zilman, A. G.; Granek, R.
- Physical Review Letters, Vol. 77, Issue 23
Interpreting Membrane Scattering Experiments at the Mesoscale: The Contribution of Dissipation within the Bilayer
journal, March 2010
- Watson, Max C.; Brown, Frank L. H.
- Biophysical Journal, Vol. 98, Issue 6
Thermal Fluctuation and Elasticity of Lipid Vesicles Interacting with Pore-Forming Peptides
journal, July 2010
- Lee, Ji-Hwan; Choi, Sung-Min; Doe, Changwoo
- Physical Review Letters, Vol. 105, Issue 3
Lipid Bilayers and Membrane Dynamics: Insight into Thickness Fluctuations
journal, July 2012
- Woodka, Andrea C.; Butler, Paul D.; Porcar, Lionel
- Physical Review Letters, Vol. 109, Issue 5
Viscosity of deuterium oxide and water in the range 5 to 125 C
journal, June 1949
- Hardy, Robert C.; Cottington, Robert L.
- Journal of Research of the National Bureau of Standards, Vol. 42, Issue 6
Probing Elastic and Viscous Properties of Phospholipid Bilayers Using Neutron Spin Echo Spectroscopy
journal, September 2017
- Nagao, Michihiro; Kelley, Elizabeth G.; Ashkar, Rana
- The Journal of Physical Chemistry Letters, Vol. 8, Issue 19
Physical properties of the fluid lipid-bilayer component of cell membranes: a perspective
journal, August 1991
- Bloom, Myer; Evans, Evan; Mouritsen, Ole G.
- Quarterly Reviews of Biophysics, Vol. 24, Issue 3
Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism
journal, April 1980
- Brahms, S.; Brahms, J.
- Journal of Molecular Biology, Vol. 138, Issue 2
Method of oriented circular dichroism
journal, April 1990
- Wu, Y.; Huang, H. W.; Olah, G. A.
- Biophysical Journal, Vol. 57, Issue 4
Fusion Activity of HIV gp41 Fusion Domain Is Related to Its Secondary Structure and Depth of Membrane Insertion in a Cholesterol-Dependent Fashion
journal, April 2012
- Lai, Alex L.; Moorthy, Anna Eswara; Li, Yinling
- Journal of Molecular Biology, Vol. 418, Issue 1-2
The third helix of the Antennapedia homeodomain translocates through biological membranes.
journal, April 1994
- Derossi, D.; Joliot, A. H.; Chassaing, G.
- Journal of Biological Chemistry, Vol. 269, Issue 14
Conformational states of the cell-penetrating peptide penetratin when interacting with phospholipid vesicles: effects of surface charge and peptide concentration
journal, June 2002
- Magzoub, Mazin; Eriksson, L. E. Göran; Gräslund, Astrid
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1563, Issue 1-2
Comparison of the interaction, positioning, structure induction and membrane perturbation of cell-penetrating peptides and non-translocating variants with phospholipid vesicles
journal, March 2003
- Magzoub, Mazin; Eriksson, L. E. Göran; Gräslund, Astrid
- Biophysical Chemistry, Vol. 103, Issue 3
Membrane-Mediated Peptide Conformation Change from α-Monomers to β-Aggregates
journal, May 2010
- Lee, Chang-Chun; Sun, Yen; Huang, Huey W.
- Biophysical Journal, Vol. 98, Issue 10
Kinetic Process of β-Amyloid Formation via Membrane Binding
journal, July 2010
- Sun, Yen; Lee, Chang-Chun; Chen, Tzu-Hsuan
- Biophysical Journal, Vol. 99, Issue 2
Effect of Chain Length and Unsaturation on Elasticity of Lipid Bilayers
journal, July 2000
- Rawicz, W.; Olbrich, K. C.; McIntosh, T.
- Biophysical Journal, Vol. 79, Issue 1
Atomic Force Microscopy Study on the Stiffness of Nanosized Liposomes Containing Charged Lipids
journal, June 2018
- Takechi-Haraya, Yuki; Goda, Yukihiro; Sakai-Kato, Kumiko
- Langmuir, Vol. 34, Issue 26
Molecular Mechanism of Peptide-Induced Pores in Membranes
journal, May 2004
- Huang, Huey W.; Chen, Fang-Yu; Lee, Ming-Tao
- Physical Review Letters, Vol. 92, Issue 19
Molecular mechanism of antimicrobial peptides: The origin of cooperativity
journal, September 2006
- Huang, Huey W.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1758, Issue 9
Entropy-Driven Softening of Fluid Lipid Bilayers by Alamethicin
journal, October 2007
- Pabst, Georg; Danner, Sabine; Podgornik, Rudi
- Langmuir, Vol. 23, Issue 23
Alamethicin influence on the membrane bending elasticity
journal, October 2005
- Vitkova, Victoria; Méléard, Philippe; Pott, Tanja
- European Biophysics Journal, Vol. 35, Issue 3
Mechanism of alamethicin insertion into lipid bilayers
journal, November 1996
- He, K.; Ludtke, S. J.; Heller, W. T.
- Biophysical Journal, Vol. 71, Issue 5
Works referencing / citing this record:
Membrane softening by nonsteroidal anti-inflammatory drugs investigated by neutron spin echo
journal, January 2019
- Sharma, V. K.; Nagao, M.; Rai, D. K.
- Physical Chemistry Chemical Physics, Vol. 21, Issue 36
High-resolution neutron spectroscopy using backscattering and neutron spin-echo spectrometers in soft and hard condensed matter
journal, January 2020
- Gardner, Jason S.; Ehlers, Georg; Faraone, Antonio
- Nature Reviews Physics, Vol. 2, Issue 2
Studies on the Interaction of Alyteserin 1c Peptide and Its Cationic Analogue with Model Membranes Imitating Mammalian and Bacterial Membranes
journal, September 2019
- Aragón-Muriel, Alberto; Ausili, Alessio; Sánchez, Kevin
- Biomolecules, Vol. 9, Issue 10
Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers
journal, January 2020
- Yu, Jing; Mao, Jun; Nagao, Michihiro
- Soft Matter, Vol. 16, Issue 4
Studies on the Interaction of Alyteserin 1c Peptide and Its Cationic Analogue with Model Membranes Imitating Mammalian and Bacterial Membranes
journal, September 2019
- Aragón-Muriel, Alberto; Ausili, Alessio; Sánchez, Kevin
- Biomolecules, Vol. 9, Issue 10