Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining
Abstract
Mechanical refining is a low-capital and well-established technology used in pulp and paper industry to improve fiber bonding for product strength. Refining can also be applied in a biorefinery context to overcome the recalcitrance of pretreated biomass by opening up the biomass structure and modifying substrate properties (e.g., morphology, particle size, porosity, crystallinity), which increases enzyme accessibility to substrate and improves carbohydrate conversion. Although several characterization methods have been used to identify the changes in substrate properties, there is no systematic approach to evaluate the extent of fiber cell wall disruption and what physical properties can explain the improvement in enzymatic digestibility when pretreated lignocellulosic biomass is mechanically refined. This is because the fiber cell wall is complex across multiple scales, including the molecular scale, nano- and meso-scale (microfibril), and microscale (tissue level). A combination of advanced characterization tools is used in this study to better understand the effect of mechanical refining on the meso-scale microfibril assembly and the relationship between those meso-scale modifications and enzymatic hydrolysis. Enzymatic conversion of autohydrolysis sugarcane bagasse was improved from 69.6 to 77.2% (11% relative increase) after applying mechanical refining and an increase in enzymatic digestibility is observed with an increase in refining intensity.more »
- Authors:
-
[1]
- North Carolina State Univ., Raleigh, NC (United States)
- Pennsylvania State Univ., University Park, PA (United States)
- Brazilian Bioethanol Science and Technology Lab. (CTBE), Campinas, SP (Brazil)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)
- OSTI Identifier:
- 1482793
- Report Number(s):
- NREL/JA-2700-72807
Journal ID: ISSN 1754-6834
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; sugarcane bagasse; autohydrolysis pretreatment; mechanical refining; enzymatic hydrolysis; fiber morphology; fiber internal delamination; cellulose crystallinity; fiber porosity
Citation Formats
de Assis, Tiago, Huang, Shixin, Driemeier, Carlos Eduardo, Donohoe, Bryon S., Kim, Chaehoon, Kim, Seong H., Gonzalez, Ronalds, Jameel, Hasan, and Park, Sunkyu. Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining. United States: N. p., 2018.
Web. doi:10.1186/s13068-018-1289-3.
de Assis, Tiago, Huang, Shixin, Driemeier, Carlos Eduardo, Donohoe, Bryon S., Kim, Chaehoon, Kim, Seong H., Gonzalez, Ronalds, Jameel, Hasan, & Park, Sunkyu. Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining. United States. https://doi.org/10.1186/s13068-018-1289-3
de Assis, Tiago, Huang, Shixin, Driemeier, Carlos Eduardo, Donohoe, Bryon S., Kim, Chaehoon, Kim, Seong H., Gonzalez, Ronalds, Jameel, Hasan, and Park, Sunkyu. Thu .
"Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining". United States. https://doi.org/10.1186/s13068-018-1289-3. https://www.osti.gov/servlets/purl/1482793.
@article{osti_1482793,
title = {Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining},
author = {de Assis, Tiago and Huang, Shixin and Driemeier, Carlos Eduardo and Donohoe, Bryon S. and Kim, Chaehoon and Kim, Seong H. and Gonzalez, Ronalds and Jameel, Hasan and Park, Sunkyu},
abstractNote = {Mechanical refining is a low-capital and well-established technology used in pulp and paper industry to improve fiber bonding for product strength. Refining can also be applied in a biorefinery context to overcome the recalcitrance of pretreated biomass by opening up the biomass structure and modifying substrate properties (e.g., morphology, particle size, porosity, crystallinity), which increases enzyme accessibility to substrate and improves carbohydrate conversion. Although several characterization methods have been used to identify the changes in substrate properties, there is no systematic approach to evaluate the extent of fiber cell wall disruption and what physical properties can explain the improvement in enzymatic digestibility when pretreated lignocellulosic biomass is mechanically refined. This is because the fiber cell wall is complex across multiple scales, including the molecular scale, nano- and meso-scale (microfibril), and microscale (tissue level). A combination of advanced characterization tools is used in this study to better understand the effect of mechanical refining on the meso-scale microfibril assembly and the relationship between those meso-scale modifications and enzymatic hydrolysis. Enzymatic conversion of autohydrolysis sugarcane bagasse was improved from 69.6 to 77.2% (11% relative increase) after applying mechanical refining and an increase in enzymatic digestibility is observed with an increase in refining intensity. Based on a combination of advanced characterizations employed in this study, it was found that the refining action caused fiber size reduction, internal delamination, and increase in pores and swellability. A higher level of delamination and higher increase in porosity, analyzed by TEM and DSC, were clearly demonstrated, which explain the faster digestibility rate during the first 72 h of enzymatic hydrolysis for disc-refined samples when compared to the PFI-refined samples. Additionally, an increased inter-fibrillar distance between cellulose microfibrils at the nano-meso-scale was also revealed by SFG analysis, while no evidence was found for a change in crystalline structure by XRD and solid-state NMR analysis.},
doi = {10.1186/s13068-018-1289-3},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {United States},
year = {Thu Oct 25 00:00:00 EDT 2018},
month = {Thu Oct 25 00:00:00 EDT 2018}
}
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Comparison of different mechanical refining technologies on the enzymatic digestibility of low severity acid pretreated corn stover
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Effects of fibrillation on the wood fibers’ enzymatic hydrolysis enhanced by mechanical refining
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Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment
journal, December 2008
- Donohoe, Bryon S.; Decker, Stephen R.; Tucker, Melvin P.
- Biotechnology and Bioengineering, Vol. 101, Issue 5
Mechanistic insights into the liquefaction stage of enzyme-mediated biomass deconstruction
journal, August 2017
- van der Zwan, Timo; Hu, Jinguang; Saddler, Jack N.
- Biotechnology and Bioengineering, Vol. 114, Issue 11
Measuring the crystallinity index of cellulose by solid state 13C nuclear magnetic resonance
journal, June 2009
- Park, Sunkyu; Johnson, David K.; Ishizawa, Claudia I.
- Cellulose, Vol. 16, Issue 4
Dynamic vapor sorption and thermoporometry to probe water in celluloses
journal, May 2012
- Driemeier, Carlos; Mendes, Fernanda M.; Oliveira, Marcelo M.
- Cellulose, Vol. 19, Issue 4
Cellulose co-crystallization and related phenomena occurring in hydrothermal treatment of sugarcane bagasse
journal, April 2015
- Driemeier, Carlos; Mendes, Fernanda M.; Santucci, Beatriz S.
- Cellulose, Vol. 22, Issue 4
Reduction of Enzyme Dosage by Oxygen Delignification and Mechanical Refining for Enzymatic Hydrolysis of Green Liquor-Pretreated Hardwood
journal, June 2011
- Koo, Bon-Wook; Treasure, Trevor H.; Jameel, Hasan
- Applied Biochemistry and Biotechnology, Vol. 165, Issue 3-4
Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: A review
journal, July 2010
- Alvira, P.; Tomás-Pejó, E.; Ballesteros, M.
- Bioresource Technology, Vol. 101, Issue 13, p. 4851-4861
Synthetic multi-component enzyme mixtures for deconstruction of lignocellulosic biomass
journal, December 2010
- Banerjee, Goutami; Car, Suzana; Scott-Craig, John S.
- Bioresource Technology, Vol. 101, Issue 23, p. 9097-9105
Comparison of different mechanical refining technologies on the enzymatic digestibility of low severity acid pretreated corn stover
journal, November 2013
- Chen, Xiaowen; Kuhn, Erik; Wang, Wei
- Bioresource Technology, Vol. 147
Enhancement in enzymatic hydrolysis by mechanical refining for pretreated hardwood lignocellulosics
journal, November 2013
- Jones, Brandon W.; Venditti, Richard; Park, Sunkyu
- Bioresource Technology, Vol. 147
Enzymatic hydrolysis of autohydrolyzed wheat straw followed by refining to produce fermentable sugars
journal, January 2014
- Ertas, Murat; Han, Qiang; Jameel, Hasan
- Bioresource Technology, Vol. 152
Production of fermentable sugars from sugarcane bagasse by enzymatic hydrolysis after autohydrolysis and mechanical refining
journal, March 2015
- Batalha, Larisse Aparecida Ribas; Han, Qiang; Jameel, Hasan
- Bioresource Technology, Vol. 180
Use of mechanical refining to improve the production of low-cost sugars from lignocellulosic biomass
journal, January 2016
- Park, Junyeong; Jones, Brandon; Koo, Bonwook
- Bioresource Technology, Vol. 199
Effects of fibrillation on the wood fibers’ enzymatic hydrolysis enhanced by mechanical refining
journal, April 2016
- Liu, Wei; Wang, Bing; Hou, Qingxi
- Bioresource Technology, Vol. 206
Changes in pore size distribution during the drying of cellulose fibers as measured by differential scanning calorimetry
journal, October 2006
- Park, S.; Venditti, R.; Jameel, H.
- Carbohydrate Polymers, Vol. 66, Issue 1
Lignin contributions to the nanoscale porosity of raw and treated lignocelluloses as observed by calorimetric thermoporometry
journal, April 2016
- Driemeier, Carlos; Oliveira, Marcelo M.; Curvelo, Antônio A. S.
- Industrial Crops and Products, Vol. 82
Multimodal Broadband Vibrational Sum Frequency Generation (MM-BB-V-SFG) Spectrometer and Microscope
journal, December 2015
- Lee, Christopher M.; Kafle, Kabindra; Huang, Shixin
- The Journal of Physical Chemistry B, Vol. 120, Issue 1
Dependence of Sum Frequency Generation (SFG) Spectral Features on the Mesoscale Arrangement of SFG-Active Crystalline Domains Interspersed in SFG-Inactive Matrix: A Case Study with Cellulose in Uniaxially Aligned Control Samples and Alkali-Treated Secondary Cell Walls of Plants
journal, May 2017
- Makarem, Mohamadamin; Sawada, Daisuke; O’Neill, Hugh M.
- The Journal of Physical Chemistry C, Vol. 121, Issue 18
Improving Sugar Yields and Reducing Enzyme Loadings in the Deacetylation and Mechanical Refining (DMR) Process through Multistage Disk and Szego Refining and Corresponding Techno-Economic Analysis
journal, December 2015
- Chen, Xiaowen; Wang, Wei; Ciesielski, Peter
- ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 1
Combined Deacetylation and PFI Refining Pretreatment of Corn Cob for the Improvement of a Two-Stage Enzymatic Hydrolysis
journal, May 2014
- Zhang, Yuedong; Mu, Xindong; Wang, Haisong
- Journal of Agricultural and Food Chemistry, Vol. 62, Issue 20
Probing crystal structure and mesoscale assembly of cellulose microfibrils in plant cell walls, tunicate tests, and bacterial films using vibrational Sum Frequency Generation (SFG) spectroscopy
journal, January 2014
- Lee, Christopher M.; Kafle, Kabindra; Park, Yong Bum
- Physical Chemistry Chemical Physics, Vol. 16, Issue 22
Monitoring Meso-Scale Ordering of Cellulose in Intact Plant Cell Walls Using Sum Frequency Generation Spectroscopy
journal, August 2013
- Park, Y. B.; Lee, C. M.; Koo, B. -W.
- PLANT PHYSIOLOGY, Vol. 163, Issue 2
Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance
journal, January 2010
- Park, Sunkyu; Baker, John O.; Himmel, Michael E.
- Biotechnology for Biofuels, Vol. 3, Issue 1
Helically agitated mixing in dry dilute acid pretreatment enhances the bioconversion of corn stover into ethanol
journal, January 2014
- He, Yanqing; Zhang, Longping; Zhang, Jian
- Biotechnology for Biofuels, Vol. 7, Issue 1
A highly efficient dilute alkali deacetylation and mechanical (disc) refining process for the conversion of renewable biomass to lower cost sugars
journal, January 2014
- Chen, Xiaowen; Shekiro, Joseph; Pschorn, Thomas
- Biotechnology for Biofuels, Vol. 7, Issue 1
Optimization of Pilot Scale Mechanical Disk Refining for Improvements in Enzymatic Digestibility of Pretreated Hardwood Lignocellulosics
journal, May 2017
- Jones, Brandon W.; Venditti, Richard; Park, Sunkyu
- BioResources, Vol. 12, Issue 3
Works referencing / citing this record:
Fiber fractionation to understand the effect of mechanical refining on fiber structure and resulting enzymatic digestibility of biomass
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- Corbett, Derek B.; Knoll, Charlie; Venditti, Richard
- Biotechnology and Bioengineering, Vol. 117, Issue 4
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Nanomechanics of cellulose deformation reveal molecular defects that facilitate natural deconstruction
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- Ciesielski, Peter N.; Wagner, Ryan; Bharadwaj, Vivek S.
- Proceedings of the National Academy of Sciences