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Title: Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass

Abstract

Neutron scattering of deuterated plants can provide fundamental insight into the structure of lignocellulosics in plant cell walls and its deconstruction by pretreatment and enzymes. Such plants need to be characterized for any alterations to lignocellulosic structure caused by growth in deuterated media. Here we show that glucose yields from enzymatic hydrolysis at lower enzyme loading were 35% and 30% for untreated deuterated and protiated switchgrass, respectively. Lignin content was 4% higher in deuterated switchgrass but there were no significant lignin structural differences. Transmission electron microscopy showed differences in lignin distribution and packing of fibers in the cell walls that apparently increased surface area of cellulose in deuterated switchgrass, increasing cellulose accessibility and lowering its recalcitrance. These differences in lignification were likely caused by abiotic stress due to growth in deuterated media.

Authors:
 [1];  [1];  [2];  [3];  [4];  [5];  [5];  [6];  [7];  [8];  [9]
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  1. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
  3. Univ. of Tennessee, Knoxville, TN (United States). Advanced Microscopy and Imaging Center
  4. Georgia Inst. of Technology, Atlanta, GA (United States). Renewable Bioproducts Inst. School of Chemistry and Biochemistry
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Carbon and Composite Group. Materials Science and Technology Division; Univ. of Tennessee, Knoxville, TN (United States). The Bredesen Center for Interdisciplinary Research and Graduate Education
  7. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
  8. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. of Biological Sciences. Biosciences Division
  9. Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering. Inst. of Agriculture. Center for Renewable Carbon. Dept. of Forestry, Wildlife, and Fisheries; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. of Biological Sciences. Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1471889
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bioalcohols; biofuels

Citation Formats

Bhagia, Samarthya, Meng, Xianzhi, Evans, Barbara R., Dunlap, John R., Bali, Garima, Chen, Jihua, Reeves, Kimberly Shawn, Ho, Hoi Chun, Davison, Brian H., Pu, Yunqiao, and Ragauskas, Arthur J. Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass. United States: N. p., 2018. Web. doi:10.1038/s41598-018-31269-w.
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Bhagia, Samarthya, Meng, Xianzhi, Evans, Barbara R., Dunlap, John R., Bali, Garima, Chen, Jihua, Reeves, Kimberly Shawn, Ho, Hoi Chun, Davison, Brian H., Pu, Yunqiao, & Ragauskas, Arthur J. Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass. United States. https://doi.org/10.1038/s41598-018-31269-w
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Bhagia, Samarthya, Meng, Xianzhi, Evans, Barbara R., Dunlap, John R., Bali, Garima, Chen, Jihua, Reeves, Kimberly Shawn, Ho, Hoi Chun, Davison, Brian H., Pu, Yunqiao, and Ragauskas, Arthur J. Wed . "Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass". United States. https://doi.org/10.1038/s41598-018-31269-w. https://www.osti.gov/servlets/purl/1471889.
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@article{osti_1471889,
title = {Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass},
author = {Bhagia, Samarthya and Meng, Xianzhi and Evans, Barbara R. and Dunlap, John R. and Bali, Garima and Chen, Jihua and Reeves, Kimberly Shawn and Ho, Hoi Chun and Davison, Brian H. and Pu, Yunqiao and Ragauskas, Arthur J.},
abstractNote = {Neutron scattering of deuterated plants can provide fundamental insight into the structure of lignocellulosics in plant cell walls and its deconstruction by pretreatment and enzymes. Such plants need to be characterized for any alterations to lignocellulosic structure caused by growth in deuterated media. Here we show that glucose yields from enzymatic hydrolysis at lower enzyme loading were 35% and 30% for untreated deuterated and protiated switchgrass, respectively. Lignin content was 4% higher in deuterated switchgrass but there were no significant lignin structural differences. Transmission electron microscopy showed differences in lignin distribution and packing of fibers in the cell walls that apparently increased surface area of cellulose in deuterated switchgrass, increasing cellulose accessibility and lowering its recalcitrance. These differences in lignification were likely caused by abiotic stress due to growth in deuterated media.},
doi = {10.1038/s41598-018-31269-w},
journal = {Scientific Reports},
number = ,
volume = 8,
place = {United States},
year = {Wed Sep 05 00:00:00 EDT 2018},
month = {Wed Sep 05 00:00:00 EDT 2018}
}
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https://doi.org/10.1038/s41598-018-31269-w
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Works referenced in this record:

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3D Chemical Image using TOF-SIMS Revealing the Biopolymer Component Spatial and Lateral Distributions in Biomass
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The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis, part 1: A modified Simons' staining technique
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The mechanism of deuterium oxide-induced protein degradation in Lemna minor
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Production of deuterated switchgrass by hydroponic cultivation
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Drilling accurate nanopores for biosensors by energetic multi-wall carbon nanotubes: a molecular dynamics investigation
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Lignification and lignin topochemistry — an ultrastructural view
journal, July 2001

Effect of in Vivo Deuteration on Structure of Switchgrass Lignin
journal, August 2017

Deactivation of Cellulase at the Air-Liquid Interface Is the Main Cause of Incomplete Cellulose Conversion at Low Enzyme Loadings
journal, January 2018

Chemical, ultrastructural and supramolecular analysis of tension wood in Populus tremula x alba as a model substrate for reduced recalcitrance
journal, January 2011

  • Foston, Marcus; Hubbell, Christopher A.; Samuel, Reichel
  • Energy & Environmental Science, Vol. 4, Issue 12
  • DOI: 10.1039/c1ee02073k

Deuterium incorporation in biomass cell wall components by NMR analysis
journal, January 2012

  • Foston, Marcus B.; McGaughey, Joseph; O'Neill, Hugh
  • The Analyst, Vol. 137, Issue 5
  • DOI: 10.1039/c2an16025k

Electron‐Diffraction Study of Water and Heavy Water
journal, February 1965

  • Shibata, Shuzo; Bartell, L. S.
  • The Journal of Chemical Physics, Vol. 42, Issue 4
  • DOI: 10.1063/1.1696094

Electron‐Diffraction Study of the Structures of C 2 H 4 and C 2 D 4
journal, April 1965

  • Bartell, L. S.; Roth, E. A.; Hollowell, C. D.
  • The Journal of Chemical Physics, Vol. 42, Issue 8
  • DOI: 10.1063/1.1703223

Growth of Lemna in H 2 O-D 2 O Mixtures: Enhancement by Kinetin
journal, September 1965

  • Cope, Benjamin T.; Bose, Shakuntala; Crespi, Henry L.
  • Botanical Gazette, Vol. 126, Issue 3
  • DOI: 10.1086/336323

Neutron Technologies for Bioenergy Research
journal, August 2012

  • Langan, Paul; Evans, Barbara R.; Foston, Marcus
  • Industrial Biotechnology, Vol. 8, Issue 4
  • DOI: 10.1089/ind.2012.0012

Plant responses to abiotic stresses: heavy metal‐induced oxidative stress and protection by mycorrhization
journal, May 2002

  • Schützendübel, Andres; Polle, Andrea
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Lignin Biosynthesis Studies in Plant Tissue Cultures
journal, February 2010

The Path Forward for Biofuels and Biomaterials
journal, January 2006

  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

Abnormal Lignin Distribution in wood from Severely Drought Stressed Pinus Radiata Trees
journal, January 2002

Techno-economic evaluation of integrated first- and second-generation ethanol production from grain and straw
journal, January 2016

  • Joelsson, Elisabeth; Erdei, Borbála; Galbe, Mats
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-015-0423-8
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    Works referencing / citing this record:

    Production of Sugar Feedstocks for Fermentation Processes from Selected Fast Growing Grasses
    journal, August 2019

    • Przybysz, Kamila; Małachowska, Edyta; Martyniak, Danuta
    • Energies, Vol. 12, Issue 16
    • DOI: 10.3390/en12163129
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