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Title: Nitrite Control over Dissimilatory Nitrate/Nitrite Reduction Pathways in Shewanella loihica Strain PV-4

Abstract

ABSTRACT Shewanella loihicastrain PV-4 harbors both a functional denitrification (NO3-→N2) and a respiratory ammonification (NO3-→NH4+) pathway. Batch and chemostat experiments revealed that NO2-affects pathway selection and the formation of reduced products. Strain PV-4 cells grown with NO2-as the sole electron acceptor produced exclusively NH4+. With NO3-as the electron acceptor, denitrification predominated and N2O accounted for ~90% of reduced products in the presence of acetylene. Additionally, chemostat experiments demonstrated that the NO2-:NO3-ratio affected the distribution of reduced products, and respiratory ammonification dominated at high NO2-:NO3-ratios, whereas low NO2-:NO3-ratios favored denitrification. The NO2-:NO3-ratios affectednirKtranscript abundance, a measure of denitrification activity, in the chemostat experiments, and cells grown at a NO2-:NO3-ratio of 3 had ~37-fold fewernirKtranscripts per cell than cells grown with NO3-as the sole electron acceptor. In contrast, the transcription ofnrfA, implicated in NO2--to-NH4+reduction, remained statistically unchanged under continuous cultivation conditions at NO2-:NO3-ratios below 3. At NO2-:NO3-ratios above 3, bothnirKandnrfAtranscript numbers decreased and the chemostat culture washed out, presumably due to NO2-toxicity. Finally, these findings implicate NO2-as a relevant modulator of NO3-fate inS. loihicastrain PV-4, and, by extension, suggest that NO2-is a relevant determinant for N retention (i.e., ammonification) versus N loss and greenhouse gas emission (i.e., denitrification).

Authors:
 [1];  [2]; ORCiD logo [3]
  1. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology and Department of Microbiology; Korea Advanced Institute of Science and Technology, Daejeon (South Korea). Department of Civil and Environmental Engineering
  2. Univ. of Illinois, Urbana, IL (United States). Department of Geology
  3. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Department of Microbiology and Department of Civil and Environmental Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). University of Tennessee and Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1470901
Grant/Contract Number:  
AC05-00OR22725; SC0006662
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 81; Journal Issue: 10; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Yoon, Sukhwan, Sanford, Robert A., and Löffler, Frank E. Nitrite Control over Dissimilatory Nitrate/Nitrite Reduction Pathways in Shewanella loihica Strain PV-4. United States: N. p., 2015. Web. doi:10.1128/AEM.00688-15.
Yoon, Sukhwan, Sanford, Robert A., & Löffler, Frank E. Nitrite Control over Dissimilatory Nitrate/Nitrite Reduction Pathways in Shewanella loihica Strain PV-4. United States. https://doi.org/10.1128/AEM.00688-15
Yoon, Sukhwan, Sanford, Robert A., and Löffler, Frank E. Fri . "Nitrite Control over Dissimilatory Nitrate/Nitrite Reduction Pathways in Shewanella loihica Strain PV-4". United States. https://doi.org/10.1128/AEM.00688-15. https://www.osti.gov/servlets/purl/1470901.
@article{osti_1470901,
title = {Nitrite Control over Dissimilatory Nitrate/Nitrite Reduction Pathways in Shewanella loihica Strain PV-4},
author = {Yoon, Sukhwan and Sanford, Robert A. and Löffler, Frank E.},
abstractNote = {ABSTRACT Shewanella loihicastrain PV-4 harbors both a functional denitrification (NO3-→N2) and a respiratory ammonification (NO3-→NH4+) pathway. Batch and chemostat experiments revealed that NO2-affects pathway selection and the formation of reduced products. Strain PV-4 cells grown with NO2-as the sole electron acceptor produced exclusively NH4+. With NO3-as the electron acceptor, denitrification predominated and N2O accounted for ~90% of reduced products in the presence of acetylene. Additionally, chemostat experiments demonstrated that the NO2-:NO3-ratio affected the distribution of reduced products, and respiratory ammonification dominated at high NO2-:NO3-ratios, whereas low NO2-:NO3-ratios favored denitrification. The NO2-:NO3-ratios affectednirKtranscript abundance, a measure of denitrification activity, in the chemostat experiments, and cells grown at a NO2-:NO3-ratio of 3 had ~37-fold fewernirKtranscripts per cell than cells grown with NO3-as the sole electron acceptor. In contrast, the transcription ofnrfA, implicated in NO2--to-NH4+reduction, remained statistically unchanged under continuous cultivation conditions at NO2-:NO3-ratios below 3. At NO2-:NO3-ratios above 3, bothnirKandnrfAtranscript numbers decreased and the chemostat culture washed out, presumably due to NO2-toxicity. Finally, these findings implicate NO2-as a relevant modulator of NO3-fate inS. loihicastrain PV-4, and, by extension, suggest that NO2-is a relevant determinant for N retention (i.e., ammonification) versus N loss and greenhouse gas emission (i.e., denitrification).},
doi = {10.1128/AEM.00688-15},
journal = {Applied and Environmental Microbiology},
number = 10,
volume = 81,
place = {United States},
year = {Fri Mar 13 00:00:00 EDT 2015},
month = {Fri Mar 13 00:00:00 EDT 2015}
}

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