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Title: Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes

Abstract

Rhodopseudomonas palustris is a species of purple photosynthetic bacteria that has a multigene family of puc genes that encode the alpha and beta apoproteins, which form the LH2 complexes. A genetic dissection strategy has been adopted in order to try and understand which spectroscopic form of LH2 these different genes produce. This paper presents a characterisation of one of the deletion mutants generated in this program, the pucBAd only mutant. This mutant produces an unusual spectroscopic form of LH2 that only has a single large NIR absorption band at 800 nm. Spectroscopic and pigment analyses on this complex suggest that it has basically a similar overall structure as that of the wild-type HL LH2 complex. The mutant has the unique phenotype where the mutant LH2 complex is only produced when cells are grown at LL. At HL the mutant only produces the LH1-RC core complex.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1];  [1]
  1. Univ. of Glasgow, Scotland (United Kingdom)
  2. Arizona State Univ., Tempe, AZ (United States)
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1470114
Grant/Contract Number:  
SC0001035
Resource Type:
Accepted Manuscript
Journal Name:
Photosynthesis Research
Additional Journal Information:
Journal Volume: 135; Journal Issue: 1-3; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; Journal ID: ISSN 0166-8595
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Photosynthesis; Purple bacteria; Puc genes; Light-harvesting; LH2

Citation Formats

Southall, June, Henry, Sarah L., Gardiner, Alastair T., Roszak, Aleksander W., Mullen, William, Carey, Anne-Marie, Kelly, Sharon M., de Percin Northumberland, Claire Ortmann, and Cogdell, Richard J. Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes. United States: N. p., 2017. Web. doi:10.1007/s11120-017-0386-7.
Southall, June, Henry, Sarah L., Gardiner, Alastair T., Roszak, Aleksander W., Mullen, William, Carey, Anne-Marie, Kelly, Sharon M., de Percin Northumberland, Claire Ortmann, & Cogdell, Richard J. Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes. United States. https://doi.org/10.1007/s11120-017-0386-7
Southall, June, Henry, Sarah L., Gardiner, Alastair T., Roszak, Aleksander W., Mullen, William, Carey, Anne-Marie, Kelly, Sharon M., de Percin Northumberland, Claire Ortmann, and Cogdell, Richard J. Wed . "Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes". United States. https://doi.org/10.1007/s11120-017-0386-7. https://www.osti.gov/servlets/purl/1470114.
@article{osti_1470114,
title = {Characterisation of a pucBA deletion mutant from Rhodopseudomonas palustris lacking all but the pucBAd genes},
author = {Southall, June and Henry, Sarah L. and Gardiner, Alastair T. and Roszak, Aleksander W. and Mullen, William and Carey, Anne-Marie and Kelly, Sharon M. and de Percin Northumberland, Claire Ortmann and Cogdell, Richard J.},
abstractNote = {Rhodopseudomonas palustris is a species of purple photosynthetic bacteria that has a multigene family of puc genes that encode the alpha and beta apoproteins, which form the LH2 complexes. A genetic dissection strategy has been adopted in order to try and understand which spectroscopic form of LH2 these different genes produce. This paper presents a characterisation of one of the deletion mutants generated in this program, the pucBAd only mutant. This mutant produces an unusual spectroscopic form of LH2 that only has a single large NIR absorption band at 800 nm. Spectroscopic and pigment analyses on this complex suggest that it has basically a similar overall structure as that of the wild-type HL LH2 complex. The mutant has the unique phenotype where the mutant LH2 complex is only produced when cells are grown at LL. At HL the mutant only produces the LH1-RC core complex.},
doi = {10.1007/s11120-017-0386-7},
journal = {Photosynthesis Research},
number = 1-3,
volume = 135,
place = {United States},
year = {Wed May 31 00:00:00 EDT 2017},
month = {Wed May 31 00:00:00 EDT 2017}
}

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Figures / Tables:

Fig. 1 Fig. 1: PCR analysis of the five pucBA gene pairs from the Rps. palustris ΔpucBAabce quadruple mutant and the wild-type strain. The two outside lanes are M-Promega 1 kb ladder marker and lanes 1–10 contain PCR fragment sizes a follows from (1) wild-type pucBAa 1498 bp, (2) ΔpucBAa 1168 bp,more » (3) wild-type pucBAb 1047 bp, (4) ΔpucBAb 1661 bp, (5) wild-type pucBAc 1280 bp, (6) ΔpucBAc 930 bp, (7) wild-type pucBAd 1246 bp, (8) ΔpucBAd 1246, (9) wildtype pucBAe 1014 bp and (10) ΔpucBAe 1488. The different sizes of the respective wild-type and deleted pucBA PCR bands from all gene pairs except pucBAd, indicate that only this gene pair is present in genomic DNA. The primer sequences are listed in Supplementary Table 3. All bands were excised and confirmed by sequencing« less

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Works referencing / citing this record:

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