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Title: A Photoactivatable BODIPY Probe for Localization-based Super-Resolution Cellular Imaging

Abstract

Abstract The synthesis and application of a photoactivatable boron‐alkylated BODIPY probe for localization‐based super‐resolution microscopy is reported. Photoactivation and excitation of the probe is achieved by a previously unknown boron‐photodealkylation reaction with a single low‐power visible laser and without requiring the addition of reducing agents or oxygen scavengers in the imaging buffer. These features lead to a versatile probe for localization‐based microscopy of biological systems. The probe can be easily linked to nucleophile‐containing molecules to target specific cellular organelles. By attaching paclitaxel to the photoactivatable BODIPY, in vitro and in vivo super‐resolution imaging of microtubules is demonstrated. This is the first example of single‐molecule localization‐based super‐resolution microscopy using a visible‐light‐activated BODIPY compound as a fluorescent probe.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
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  1. Iowa State Univ. of Science and Technology, Ames, IA (United States)
Publication Date:
Research Org.:
Ames Laboratory (AMES), Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1464487
Alternate Identifier(s):
OSTI ID: 1468373
Report Number(s):
IS-J-9578
Journal ID: ISSN 1433-7851
Grant/Contract Number:  
AC02-07CH11358
Resource Type:
Accepted Manuscript
Journal Name:
Angewandte Chemie (International Edition)
Additional Journal Information:
Journal Name: Angewandte Chemie (International Edition); Journal Volume: 57; Journal Issue: 39; Journal ID: ISSN 1433-7851
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; bodipy; fluorescence; imaging agents; photoactivation; super-resolution microscopy

Citation Formats

Wijesooriya, Chamari S., Peterson, Julie A., Shrestha, Pradeep, Gehrmann, Elizabeth J., Winter, Arthur H., and Smith, Emily. A Photoactivatable BODIPY Probe for Localization-based Super-Resolution Cellular Imaging. United States: N. p., 2018. Web. doi:10.1002/anie.201805827.
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Wijesooriya, Chamari S., Peterson, Julie A., Shrestha, Pradeep, Gehrmann, Elizabeth J., Winter, Arthur H., & Smith, Emily. A Photoactivatable BODIPY Probe for Localization-based Super-Resolution Cellular Imaging. United States. https://doi.org/10.1002/anie.201805827
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Wijesooriya, Chamari S., Peterson, Julie A., Shrestha, Pradeep, Gehrmann, Elizabeth J., Winter, Arthur H., and Smith, Emily. Wed . "A Photoactivatable BODIPY Probe for Localization-based Super-Resolution Cellular Imaging". United States. https://doi.org/10.1002/anie.201805827. https://www.osti.gov/servlets/purl/1464487.
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@article{osti_1464487,
title = {A Photoactivatable BODIPY Probe for Localization-based Super-Resolution Cellular Imaging},
author = {Wijesooriya, Chamari S. and Peterson, Julie A. and Shrestha, Pradeep and Gehrmann, Elizabeth J. and Winter, Arthur H. and Smith, Emily},
abstractNote = {Abstract The synthesis and application of a photoactivatable boron‐alkylated BODIPY probe for localization‐based super‐resolution microscopy is reported. Photoactivation and excitation of the probe is achieved by a previously unknown boron‐photodealkylation reaction with a single low‐power visible laser and without requiring the addition of reducing agents or oxygen scavengers in the imaging buffer. These features lead to a versatile probe for localization‐based microscopy of biological systems. The probe can be easily linked to nucleophile‐containing molecules to target specific cellular organelles. By attaching paclitaxel to the photoactivatable BODIPY, in vitro and in vivo super‐resolution imaging of microtubules is demonstrated. This is the first example of single‐molecule localization‐based super‐resolution microscopy using a visible‐light‐activated BODIPY compound as a fluorescent probe.},
doi = {10.1002/anie.201805827},
journal = {Angewandte Chemie (International Edition)},
number = 39,
volume = 57,
place = {United States},
year = {Wed Jul 18 00:00:00 EDT 2018},
month = {Wed Jul 18 00:00:00 EDT 2018}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1002/anie.201805827
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Cited by: 71 works
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Table 1 Table 1: Photophysical properties of compounds 1-3
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