PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis
Abstract
Upregulation of sphingosine-1-phosphate receptor 1 (S1PR1) expression in multiple sclerosis (MS) lesions is associated with neuroinflammatory response. This study investigated the correlation between neuroinflammation and S1PR1 expression in the spinal cord of an experimental autoimmune encephalomyelitis (EAE) rat model of MS, using the S1PR1 positron emission tomography (PET) radiotracer [11C]TZ3321. MicroPET imaging studies of [11C]TZ3321 were performed to measure uptake of [11C]TZ3321 in the spinal cord of EAE rats. Immunohistochemical staining was performed to confirm the overexpression of S1PR1 and other inflammatory biomarkers. MicroPET imaging demonstrated a 20–30 % increase in [11C]TZ3321 uptake in the lumbar spinal cord of EAE rats versus sham controls at 35–60 min post injection. The increased uptake of [11C]TZ3321 was correlated with the overexpression of S1PR1 in the lumbar spinal cord of EAE rats that was confirmed by immunohistochemical staining. Upregulated S1PR1 expression was associated with glial cell activation and immune cell infiltration. MicroPET imaging modality with a specific radioligand [11C]TZ3321 is able to assess the expression of S1PR1 in EAE rat lumbar spinal cord. This may provide a new approach to the assessment of neuroinflammatory response in MS and other inflammatory diseases.
- Authors:
-
- Washington Univ. School of Medicine, St. Louis, MO (United States)
- Publication Date:
- Research Org.:
- Washington Univ., St. Louis, MO (United States). School of Medicine
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1463267
- Grant/Contract Number:
- SC0008432
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Molecular Imaging and Biology
- Additional Journal Information:
- Journal Volume: 18; Journal Issue: 5; Journal ID: ISSN 1536-1632
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Liu, Hui, Jin, Hongjun, Yue, Xuyi, Luo, Zonghua, Liu, Chunling, Rosenberg, Adam J., and Tu, Zhude. PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis. United States: N. p., 2016.
Web. doi:10.1007/s11307-016-0944-y.
Liu, Hui, Jin, Hongjun, Yue, Xuyi, Luo, Zonghua, Liu, Chunling, Rosenberg, Adam J., & Tu, Zhude. PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis. United States. https://doi.org/10.1007/s11307-016-0944-y
Liu, Hui, Jin, Hongjun, Yue, Xuyi, Luo, Zonghua, Liu, Chunling, Rosenberg, Adam J., and Tu, Zhude. Mon .
"PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis". United States. https://doi.org/10.1007/s11307-016-0944-y. https://www.osti.gov/servlets/purl/1463267.
@article{osti_1463267,
title = {PET Imaging Study of S1PR1 Expression in a Rat Model of Multiple Sclerosis},
author = {Liu, Hui and Jin, Hongjun and Yue, Xuyi and Luo, Zonghua and Liu, Chunling and Rosenberg, Adam J. and Tu, Zhude},
abstractNote = {Upregulation of sphingosine-1-phosphate receptor 1 (S1PR1) expression in multiple sclerosis (MS) lesions is associated with neuroinflammatory response. This study investigated the correlation between neuroinflammation and S1PR1 expression in the spinal cord of an experimental autoimmune encephalomyelitis (EAE) rat model of MS, using the S1PR1 positron emission tomography (PET) radiotracer [11C]TZ3321. MicroPET imaging studies of [11C]TZ3321 were performed to measure uptake of [11C]TZ3321 in the spinal cord of EAE rats. Immunohistochemical staining was performed to confirm the overexpression of S1PR1 and other inflammatory biomarkers. MicroPET imaging demonstrated a 20–30 % increase in [11C]TZ3321 uptake in the lumbar spinal cord of EAE rats versus sham controls at 35–60 min post injection. The increased uptake of [11C]TZ3321 was correlated with the overexpression of S1PR1 in the lumbar spinal cord of EAE rats that was confirmed by immunohistochemical staining. Upregulated S1PR1 expression was associated with glial cell activation and immune cell infiltration. MicroPET imaging modality with a specific radioligand [11C]TZ3321 is able to assess the expression of S1PR1 in EAE rat lumbar spinal cord. This may provide a new approach to the assessment of neuroinflammatory response in MS and other inflammatory diseases.},
doi = {10.1007/s11307-016-0944-y},
journal = {Molecular Imaging and Biology},
number = 5,
volume = 18,
place = {United States},
year = {Mon Mar 14 00:00:00 EDT 2016},
month = {Mon Mar 14 00:00:00 EDT 2016}
}
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